Objective:To investigate the effects of intrauterine infection in different periods on the placenta and endometrial blood vessel formation of pregnant rats and the growth and development of fetal rats.Methods:Accordin...Objective:To investigate the effects of intrauterine infection in different periods on the placenta and endometrial blood vessel formation of pregnant rats and the growth and development of fetal rats.Methods:According to the random number table method,32 pregnant rats were divided into the early infection group,the mid-term infection group,the late infection group and the control group,with 8 rats in each group.On the 3rd,9th and 15th day of pregnancy,lipopolysaccharide was injected intraperitoneally to construct intrauterine infection models.The pregnant rats in the control group were intraperitoneally injected with the same dose of 0.9%sodium chloride solution.On the 18th day of pregnancy,the inflammatory factors[interleukin-6(IL-6),tumor necrosis factor-(TNF-)],the blood vessel density of placenta and endometrium in the placental tissues of pregnant rats,dead fetus+absorbed fetus,the inflammatory factors IL-6,TNF-and oxidation reaction indicators[malondialdehyde(MDA)and myeloperoxidase(MPO)]in the fetal rat lung and brain tissues were detected.Results:The changing trend of IL-6 and TNF-levels in the placental tissues of pregnant rats with intrauterine infection in different periods was:the control group<the late infection group<the mid-term infection group<the early infection group,the differences were statistically significant(p<.05).The changing trend of fetal rat weight,placental weight and placental coefficient in the intrauterine infection groups in different periods was:the control group>the late infection group>the mid-term infection group>the early infection group,the differences were statistically significant(p<.05).The blood vessel density of placenta and endometrium,the mean number of fetuses,brain coefficient and lung coefficient in the late infection group were significantly increased in comparison with the early infection group and the mid-term infection group.The total number and the ratio of dead fetus+absorbed fetus,the levels of IL-6,TNF-,MDA and MPO in brain and lung tissues were significantly reduced,and the differences were statistically significant(p<.05).The blood vessel density of placenta and endometrium,brain coefficient and lung coefficient of pregnant rats in the mid-term infection group were significantly increased in comparison with the early infection group,and the differences were statistically significant(p<.05).There was no statistically significant difference in the other indicators between the two groups(p>.05).Conclusions:Intrauterine infection in different periods can inhibit placental and endometrial angiogenesis,and affect the survival rate of fetal rats and the growth and development of brain and lung.The reason may be related to the aggravation of fetal inflammatory responses and oxidative stress.The earlier the intrauterine infection occurs,the severer the adverse effects on the fetal rats will be.展开更多
Nocistatin was synthesized by the solid-phase peptide synthesis method. Its effects on rat systemic arterial pressure; rat hindquarter vascular bed resistance; tension of rabbit pectoral, abdominal, femoral aorta musc...Nocistatin was synthesized by the solid-phase peptide synthesis method. Its effects on rat systemic arterial pressure; rat hindquarter vascular bed resistance; tension of rabbit pectoral, abdominal, femoral aorta muscle strips without endothelium; and nociceptin induced decreases of rat systemic arterial pressure were determined. The results showed that nocistatin can increase the systemic arterial pressure, increase the hindquarter vascular bed resistance and induce the contraction significantly of abdominal, femoral aorta muscle strips without endotheiium; it has no significant effect on tension of pectoral aorta muscle strips, it cannot antagonize significantly the decrease of rat systemic arterial pressure induced by nociceptin. These results suggest that nocistatin has some important effects on blood vessel activities.展开更多
Objective:To investigate the effects of ginger root juice on contractibility of resistance blood vessels from mice and on activation of extracellular signal-regulated kinase 1/2(ERK1/2)and endothelial nitric oxide syn...Objective:To investigate the effects of ginger root juice on contractibility of resistance blood vessels from mice and on activation of extracellular signal-regulated kinase 1/2(ERK1/2)and endothelial nitric oxide synthase(eNOS)in human endothelial cells.Methods:Juice was prepared from semi-dry ginger roots.Mesenteric artery rings were dis-sected from healthy adult C57BL/J6 mice.Primary human umbilical vein endothelial cells(HU-VECs)were isolated from umbilical cords of normal full-term babies.The contractibility of the dissected vessel rings in the presence or absence of ginger root juice at 0.1%(v/v)after potassium(100 mM KCL)stimulation was measured by wire myography.The phosphorylation levels of ERK1/2 and eNOS in the presence of ginger root juice in the culture medium at 0,0.025%,0.05%,0.1%,and 0.2%(v/v)in HUVECs were assessed by western blotting analysis.Results:An immediate sharp increase in the contractile activity was observed in mesenteric artery rings in response to KCL stimulation.Ginger root juice effectively attenuated the KCL-mediated vessel contraction.Moreover,ginger root juice significantly increased phosphorylation of ERK1/2 and eNOS in a dose-dependent manner.Conclusions:Ginger root juice is capable of relaxing resistance blood vessels.Activation of ERK1/2 and eNOS through phosphorylation in endothelial cells may be a mechanism underlying the vasodilator activity of ginger root.展开更多
文摘Objective:To investigate the effects of intrauterine infection in different periods on the placenta and endometrial blood vessel formation of pregnant rats and the growth and development of fetal rats.Methods:According to the random number table method,32 pregnant rats were divided into the early infection group,the mid-term infection group,the late infection group and the control group,with 8 rats in each group.On the 3rd,9th and 15th day of pregnancy,lipopolysaccharide was injected intraperitoneally to construct intrauterine infection models.The pregnant rats in the control group were intraperitoneally injected with the same dose of 0.9%sodium chloride solution.On the 18th day of pregnancy,the inflammatory factors[interleukin-6(IL-6),tumor necrosis factor-(TNF-)],the blood vessel density of placenta and endometrium in the placental tissues of pregnant rats,dead fetus+absorbed fetus,the inflammatory factors IL-6,TNF-and oxidation reaction indicators[malondialdehyde(MDA)and myeloperoxidase(MPO)]in the fetal rat lung and brain tissues were detected.Results:The changing trend of IL-6 and TNF-levels in the placental tissues of pregnant rats with intrauterine infection in different periods was:the control group<the late infection group<the mid-term infection group<the early infection group,the differences were statistically significant(p<.05).The changing trend of fetal rat weight,placental weight and placental coefficient in the intrauterine infection groups in different periods was:the control group>the late infection group>the mid-term infection group>the early infection group,the differences were statistically significant(p<.05).The blood vessel density of placenta and endometrium,the mean number of fetuses,brain coefficient and lung coefficient in the late infection group were significantly increased in comparison with the early infection group and the mid-term infection group.The total number and the ratio of dead fetus+absorbed fetus,the levels of IL-6,TNF-,MDA and MPO in brain and lung tissues were significantly reduced,and the differences were statistically significant(p<.05).The blood vessel density of placenta and endometrium,brain coefficient and lung coefficient of pregnant rats in the mid-term infection group were significantly increased in comparison with the early infection group,and the differences were statistically significant(p<.05).There was no statistically significant difference in the other indicators between the two groups(p>.05).Conclusions:Intrauterine infection in different periods can inhibit placental and endometrial angiogenesis,and affect the survival rate of fetal rats and the growth and development of brain and lung.The reason may be related to the aggravation of fetal inflammatory responses and oxidative stress.The earlier the intrauterine infection occurs,the severer the adverse effects on the fetal rats will be.
基金the National NaturalScience Foundation of China (Grant No. 29972016) and the Natural Science Foundation of Gansu Province (Grant No. ZS 991-A23-056).
文摘Nocistatin was synthesized by the solid-phase peptide synthesis method. Its effects on rat systemic arterial pressure; rat hindquarter vascular bed resistance; tension of rabbit pectoral, abdominal, femoral aorta muscle strips without endothelium; and nociceptin induced decreases of rat systemic arterial pressure were determined. The results showed that nocistatin can increase the systemic arterial pressure, increase the hindquarter vascular bed resistance and induce the contraction significantly of abdominal, femoral aorta muscle strips without endotheiium; it has no significant effect on tension of pectoral aorta muscle strips, it cannot antagonize significantly the decrease of rat systemic arterial pressure induced by nociceptin. These results suggest that nocistatin has some important effects on blood vessel activities.
基金This research received no specific grant from any funding agency in the public,commercial,or not-for-profit sectors.
文摘Objective:To investigate the effects of ginger root juice on contractibility of resistance blood vessels from mice and on activation of extracellular signal-regulated kinase 1/2(ERK1/2)and endothelial nitric oxide synthase(eNOS)in human endothelial cells.Methods:Juice was prepared from semi-dry ginger roots.Mesenteric artery rings were dis-sected from healthy adult C57BL/J6 mice.Primary human umbilical vein endothelial cells(HU-VECs)were isolated from umbilical cords of normal full-term babies.The contractibility of the dissected vessel rings in the presence or absence of ginger root juice at 0.1%(v/v)after potassium(100 mM KCL)stimulation was measured by wire myography.The phosphorylation levels of ERK1/2 and eNOS in the presence of ginger root juice in the culture medium at 0,0.025%,0.05%,0.1%,and 0.2%(v/v)in HUVECs were assessed by western blotting analysis.Results:An immediate sharp increase in the contractile activity was observed in mesenteric artery rings in response to KCL stimulation.Ginger root juice effectively attenuated the KCL-mediated vessel contraction.Moreover,ginger root juice significantly increased phosphorylation of ERK1/2 and eNOS in a dose-dependent manner.Conclusions:Ginger root juice is capable of relaxing resistance blood vessels.Activation of ERK1/2 and eNOS through phosphorylation in endothelial cells may be a mechanism underlying the vasodilator activity of ginger root.
