A laser scanning confocal imaging-surface plasmon resonance （LSCI-SPR） instrument integrated with a wavelength-dependent surface plasmon resonance （SPR） sensor and a laser scanning confocal microscopy （LSCM） is ...A laser scanning confocal imaging-surface plasmon resonance （LSCI-SPR） instrument integrated with a wavelength-dependent surface plasmon resonance （SPR） sensor and a laser scanning confocal microscopy （LSCM） is built to detect the bonding process of human IgG and fluorescent-labeled affinity purified antibodies in real time. The shifts of resonant wavelength at different reaction time stages are obtained by SPR, corresponding well with the changes of the fluorescence intensity collected by using LSCM. The instrument shows the merits of the combination and complementation of the SPR and LSCM, with such advantages as quantificational analysis, high spatial resolution and real time monitor, which are of great importance for practical applications in biosensor and life science.展开更多
Five phospholipids in human placenta were determined by phosphorus 31 nuclear magnetic resonance(^(31)P NMR)spectroscopy and thin-layer chromatography(TLC) scanning combined with the corrective method of absorbance pr...Five phospholipids in human placenta were determined by phosphorus 31 nuclear magnetic resonance(^(31)P NMR)spectroscopy and thin-layer chromatography(TLC) scanning combined with the corrective method of absorbance proportional coefficient. The NMR spectrometer used this investigation was a Bruker AM-500 spectrometer operating at 202.4 MHz for ^(31)P chemical shifts are relative to 85% phosphoric acid. TIC was carried out by silica gel H plate developed in chloroform-methanol-glacial acetic acid-ethanol-water(25:4:6:2:0.5),with Vaskovsky reagent as colour -developing agent of phospholipids.展开更多
A scanning near field acoustic microscopy(SNAM) used in imaging the topography of precise surfaces is presented. As a micro force sensor, a non encapsulated 1 MHz quartz resonator is damped by hydrodynamic forces w...A scanning near field acoustic microscopy(SNAM) used in imaging the topography of precise surfaces is presented. As a micro force sensor, a non encapsulated 1 MHz quartz resonator is damped by hydrodynamic forces when approaching an object. Thus the oscillating characteristics of the leg of the quartz resonator change. While measuring the decrease of the amplitude of vibrating, the shape of the object can be obtained. Based on the analyses of the principle and key parameters of SNAM, a SNAM is exploited. A lateral resolution of 0.5 μm and vertical resolution of 2 nm has been achieved in the experiments.展开更多
This Letter proposes a scanned holographic display system that takes the advantage of a high-speed resonant scanner to augment a galvanometer and hence improves the opto-mechanical information distribution capabilitie...This Letter proposes a scanned holographic display system that takes the advantage of a high-speed resonant scanner to augment a galvanometer and hence improves the opto-mechanical information distribution capabilities,thereby potentially achieving an increased image size and enlarged viewing angles.展开更多
基金supported by the Instrument Developing Project of the Chinese Academy of Sciences (Grant No.YZ200740)the National Natural Science Foundation of China (Grant Nos.60978034 and 10974019)the National High Technology Research and Development Program of China (Grant No.2009AA03Z318)
文摘A laser scanning confocal imaging-surface plasmon resonance （LSCI-SPR） instrument integrated with a wavelength-dependent surface plasmon resonance （SPR） sensor and a laser scanning confocal microscopy （LSCM） is built to detect the bonding process of human IgG and fluorescent-labeled affinity purified antibodies in real time. The shifts of resonant wavelength at different reaction time stages are obtained by SPR, corresponding well with the changes of the fluorescence intensity collected by using LSCM. The instrument shows the merits of the combination and complementation of the SPR and LSCM, with such advantages as quantificational analysis, high spatial resolution and real time monitor, which are of great importance for practical applications in biosensor and life science.
文摘Five phospholipids in human placenta were determined by phosphorus 31 nuclear magnetic resonance(^(31)P NMR)spectroscopy and thin-layer chromatography(TLC) scanning combined with the corrective method of absorbance proportional coefficient. The NMR spectrometer used this investigation was a Bruker AM-500 spectrometer operating at 202.4 MHz for ^(31)P chemical shifts are relative to 85% phosphoric acid. TIC was carried out by silica gel H plate developed in chloroform-methanol-glacial acetic acid-ethanol-water(25:4:6:2:0.5),with Vaskovsky reagent as colour -developing agent of phospholipids.
基金This project is supported by Doctoral Grant of National Education Ministry (No.97048707).
文摘A scanning near field acoustic microscopy(SNAM) used in imaging the topography of precise surfaces is presented. As a micro force sensor, a non encapsulated 1 MHz quartz resonator is damped by hydrodynamic forces when approaching an object. Thus the oscillating characteristics of the leg of the quartz resonator change. While measuring the decrease of the amplitude of vibrating, the shape of the object can be obtained. Based on the analyses of the principle and key parameters of SNAM, a SNAM is exploited. A lateral resolution of 0.5 μm and vertical resolution of 2 nm has been achieved in the experiments.
基金part of the COIN-3DⅡproject as a collaboration between the University of Cambridge and Disney Research under the CAPE consortium
文摘This Letter proposes a scanned holographic display system that takes the advantage of a high-speed resonant scanner to augment a galvanometer and hence improves the opto-mechanical information distribution capabilities,thereby potentially achieving an increased image size and enlarged viewing angles.
