Backgrounds: Pseudomonas aeruginosa is a classic opportunistic pathogen with innate resistance to many antibiotics and disinfectants. The lung is a main target for colonization and infection by the bacteria either in ...Backgrounds: Pseudomonas aeruginosa is a classic opportunistic pathogen with innate resistance to many antibiotics and disinfectants. The lung is a main target for colonization and infection by the bacteria either in the context of a chronic, progressively deteriorating infectious and inflammatory pulmonary disease such as cystic fibrosis (CF) or in a more acute setting such as severe pneumonia in immunocompromised patients [1]. Aim and Objectives: To study the prevalence, virulence and the resistance pattern, phenotypic and genotypic characterization of P. aeruginosa from sputum samples. Materials and Methods: The present study was carried out with a total of 500 clinical sputum samples, which were received from patients, admitted to the various departments of Rajah Muthiah Medical College & Hospital, Annamalai University, Chidambaram. Result: Of the 500 samples subjected for isolation and identification of P. aeruginosa, 116 (23.20%) were positive. The isolated strains were tested for antibiotic sensitivity patterns. 93.10% of P. aeruginosa showed a maximum sensitivity to Ofloxacin, Norfloxacin and 86.20% of strains were highly resistant to Cefotaxime. The same isolates were also tested for phenotypic characterization of Extended Spectrum of Beta Lactamases by double disc synergy method against Cefotaxime and Clavulanic acid, according to the criteria of Hi-Media [2]. Of the resistant strains of P. aeruginosa isolated from sputum, 59% were positive for ESBL. The genotype characterization of ESBL P. aeruginosa showed 40% of CTX-M and 46.66% SHV gene. Conclusion: The present study strongly recommends for further checking of the antibiotic resistant strains of P. aeruginosa for phenotypic characterization of ESBL for effective treatment.展开更多
目的了解老年人医院下呼吸道感染(nosocom ia l low er resp iratory tract in fection NLRT I)的危险因素及特点。方法对2004年1月-12月在我科住院的25例(37例次)NLRT I进行回顾性分析。结果感染发生率3.94%,高龄、免疫力低下、多种基...目的了解老年人医院下呼吸道感染(nosocom ia l low er resp iratory tract in fection NLRT I)的危险因素及特点。方法对2004年1月-12月在我科住院的25例(37例次)NLRT I进行回顾性分析。结果感染发生率3.94%,高龄、免疫力低下、多种基础疾病、营养不良、长期住院、侵入性操作、反复使用广谱抗生素等是易感危险因素,感染细菌以革兰氏阴性菌为主,耐药率高是老年人NLRT I的特点。结论针对危险因素及相关环节制定有力措施,可降低老年人NLRT I。展开更多
文摘Backgrounds: Pseudomonas aeruginosa is a classic opportunistic pathogen with innate resistance to many antibiotics and disinfectants. The lung is a main target for colonization and infection by the bacteria either in the context of a chronic, progressively deteriorating infectious and inflammatory pulmonary disease such as cystic fibrosis (CF) or in a more acute setting such as severe pneumonia in immunocompromised patients [1]. Aim and Objectives: To study the prevalence, virulence and the resistance pattern, phenotypic and genotypic characterization of P. aeruginosa from sputum samples. Materials and Methods: The present study was carried out with a total of 500 clinical sputum samples, which were received from patients, admitted to the various departments of Rajah Muthiah Medical College & Hospital, Annamalai University, Chidambaram. Result: Of the 500 samples subjected for isolation and identification of P. aeruginosa, 116 (23.20%) were positive. The isolated strains were tested for antibiotic sensitivity patterns. 93.10% of P. aeruginosa showed a maximum sensitivity to Ofloxacin, Norfloxacin and 86.20% of strains were highly resistant to Cefotaxime. The same isolates were also tested for phenotypic characterization of Extended Spectrum of Beta Lactamases by double disc synergy method against Cefotaxime and Clavulanic acid, according to the criteria of Hi-Media [2]. Of the resistant strains of P. aeruginosa isolated from sputum, 59% were positive for ESBL. The genotype characterization of ESBL P. aeruginosa showed 40% of CTX-M and 46.66% SHV gene. Conclusion: The present study strongly recommends for further checking of the antibiotic resistant strains of P. aeruginosa for phenotypic characterization of ESBL for effective treatment.
文摘目的了解老年人医院下呼吸道感染(nosocom ia l low er resp iratory tract in fection NLRT I)的危险因素及特点。方法对2004年1月-12月在我科住院的25例(37例次)NLRT I进行回顾性分析。结果感染发生率3.94%,高龄、免疫力低下、多种基础疾病、营养不良、长期住院、侵入性操作、反复使用广谱抗生素等是易感危险因素,感染细菌以革兰氏阴性菌为主,耐药率高是老年人NLRT I的特点。结论针对危险因素及相关环节制定有力措施,可降低老年人NLRT I。
