3D bioprinting of complex biological structures with tunable elastic modulus and porosity using freeform reversible embedding of suspended hydrogels

Three-dimensional(3D)bioprinting has been used widely for the construction of hard tissues such as bone and cartilage.However,constructing soft tissues with complex structures remains a challenge.In this study,complex structures characterized by both tunable elastic modulus and porosity were printed using freeform reversible embedding of suspended hydrogels(FRESHs)printing methods.A mixture of alginate and gelatin was used as the main functional component of the bioink.Rheological analysis showed that this bioink possesses shear thinning and shear recovery properties,supporting both cryogenic and FRESH printing methods.Potential printing capabilities and limitations of cryogenic and FRESH printing were then analyzed by printability tests.A series of complex structures were printed by FRESH printing methods which could not be realized using conventional approaches.Mechanical tests and scanning electron microscopy analysis showed that the printed structure is of excellent flexibility and could be applied in various conditions by adjusting its mechanical modulus and porosity.L929 fibroblast cells maintained cell viability in cell-laden-printed structures,and the addition of collagen further improved the hydrogels’biocompatibility.Overall,all results provided useful insight into the building of human soft tissue organ blocks.

Reversible Data Embedding Scheme Using Relationships between Pixel-Values and Their Neighbors

Reversible data embedding is becoming a very important issue in securing images transmitted over the Internet, especially in dealing with sensitive images such as those created for military data and medical data. Based on the relationships between pixels and their neighbors, we propose a reversible data embedding scheme to embed hidden messages into an original image. In our proposed scheme, a two-layer data embedding approach is used for our proposed data embedding phase. Layer-1 embedding is used to hide secret data. Layer-2 embedding, which is an embedding variant of the proposed layer-1 embedding, is used to hide side information, such as the parameters required to restore the marked image. In our layer-1 embedding, the value of an embedded pixel is determined according to a predetermined threshold and the relationship between the pixel and its neighbors. In our layer-2 embedding, the similar data embedding concept is expanded to the block-based. Experimental results provide supportive data to show that the proposed scheme can provide greater hiding capacity while preserving the image quality of a marked image in comparison with previous work.

Reversible Data Hiding for Medical Images in Cloud Computing Environments Based on Chaotic Hénon Map

Reversible data hiding techniques are capable of reconstructing the original cover image from stego-images. Recently, many researchers have focused on reversible data hiding to protect intellectual property rights. In this paper, we combine reversible data hiding with the chaotic Henon map as an encryption technique to achieve an acceptable level of confidentiality in cloud computing environments. And, Haar digital wavelet transformation （HDWT） is also applied to convert an image from a spatial domain into a frequency domain. And then the decimal of coefficients and integer of high frequency band are modified for hiding secret bits. Finally, the modified coefficients are inversely transformed to stego-images.