Introduction The cyano branched carbohydrates are useful, versatil intermediaries for the synthesis of branched sugars having biological activity in nature. Owing to the good yield, mild reaction conditions and high s...Introduction The cyano branched carbohydrates are useful, versatil intermediaries for the synthesis of branched sugars having biological activity in nature. Owing to the good yield, mild reaction conditions and high stereospecificity, the phase transition catalysis(PTC)method has been widely used to the syntheses of C-展开更多
Data security is a major cloud computing issue due to different usertransactions in the system. The evolution of cryptography and cryptographic analysis are regarded domains of current research. deoxyribo nucleic acid...Data security is a major cloud computing issue due to different usertransactions in the system. The evolution of cryptography and cryptographic analysis are regarded domains of current research. deoxyribo nucleic acid (DNA) cryptography makes use of DNA as a sensing platform, which is then manipulated usinga variety of molecular methods. Many security mechanisms including knowledgebased authentication, two-factor authentication, adaptive authentication, multifactorauthentication and single password authentication have been deployed. These cryptographic techniques have been developed to ensure confidentiality, but most ofthem are based on complex mathematical calculations and equations. In the proposed approach, a novel and unique Hybrid helix scuttle-deoxy ribo nucleic acids(HHS-DNA) encryption algorithm has been proposed which is inspired by DNAcryptography and Helix scuttle. The proposed HHS-DNA is a type of multifold binary version of DNA (MF-BDNA). The major role of this paper is to present a multifold HHS-DNA algorithm to encrypt the cloud data assuring more security with lesscomplexity. The experimentation is carried out and it reduces the encryption time,cipher text size, and improves throughput. When compared with previous techniques, there is a 45% improvement in throughput, 37% fast in encryption time,54.67% cipher text size. The relevant experimental results and foregoing analysisshow that this method is of good robustness, stability, and security.展开更多
Sequences of circular RNAs(circ RNAs)produced from back-splicing of exon(s)completely overlap with those from cognate linear RNAs transcribed from the same gene loci with the exception of their back-splicing junction(...Sequences of circular RNAs(circ RNAs)produced from back-splicing of exon(s)completely overlap with those from cognate linear RNAs transcribed from the same gene loci with the exception of their back-splicing junction(BSJ)sites.Therefore,examination of global circ RNA expression from RNA-seq datasets generally relies on the detection of RNA-seq fragments spanning BSJ sites,which is different from the quantification of linear RNA expression by normalized RNA-seq fragments mapped to whole gene bodies.Thus,direct comparison of circular and linear RNA expression from the same gene loci in a genome-wide manner has remained challenging.Here,we update the previously-reported CIRCexplorer pipeline to version 3 for circular and linear RNA expression analysis from ribosomal-RNA depleted RNA-seq(CIRCexplorer3-CLEAR).A new quantitation parameter,fragments per billion mapped bases(FPB),is applied to evaluate circular and linear RNA expression individually by fragments mapped to circ RNA-specific BSJ sites or to linear RNA-specific splicing junction(SJ)sites.Comparison of circular and linear RNA expression levels is directly achieved by dividing FPBcircby FPBlinearto generate a CIRCscore,which indicates the relative circ RNA expression level using linear RNA expression level as the background.Highlyexpressed circ RNAs with low cognate linear RNA expression background can be readily identified by CIRCexplorer3-CLEAR for further investigation.CIRCexplorer3-CLEAR is publically available at https://github.com/Yang Lab/CLEAR.展开更多
基金Supported by the National Natural Science Foundation of China
文摘Introduction The cyano branched carbohydrates are useful, versatil intermediaries for the synthesis of branched sugars having biological activity in nature. Owing to the good yield, mild reaction conditions and high stereospecificity, the phase transition catalysis(PTC)method has been widely used to the syntheses of C-
文摘Data security is a major cloud computing issue due to different usertransactions in the system. The evolution of cryptography and cryptographic analysis are regarded domains of current research. deoxyribo nucleic acid (DNA) cryptography makes use of DNA as a sensing platform, which is then manipulated usinga variety of molecular methods. Many security mechanisms including knowledgebased authentication, two-factor authentication, adaptive authentication, multifactorauthentication and single password authentication have been deployed. These cryptographic techniques have been developed to ensure confidentiality, but most ofthem are based on complex mathematical calculations and equations. In the proposed approach, a novel and unique Hybrid helix scuttle-deoxy ribo nucleic acids(HHS-DNA) encryption algorithm has been proposed which is inspired by DNAcryptography and Helix scuttle. The proposed HHS-DNA is a type of multifold binary version of DNA (MF-BDNA). The major role of this paper is to present a multifold HHS-DNA algorithm to encrypt the cloud data assuring more security with lesscomplexity. The experimentation is carried out and it reduces the encryption time,cipher text size, and improves throughput. When compared with previous techniques, there is a 45% improvement in throughput, 37% fast in encryption time,54.67% cipher text size. The relevant experimental results and foregoing analysisshow that this method is of good robustness, stability, and security.
基金the Strategic Priority Research Program of Chinese Academy of Sciences,China(Grant No.XDB19020104)the National Natural Science Foundation of China(Grant Nos.31730111,31925011,and 91940306)the Howard Hughes Medical Institute International Program,the United States(Grant No.55008728).
文摘Sequences of circular RNAs(circ RNAs)produced from back-splicing of exon(s)completely overlap with those from cognate linear RNAs transcribed from the same gene loci with the exception of their back-splicing junction(BSJ)sites.Therefore,examination of global circ RNA expression from RNA-seq datasets generally relies on the detection of RNA-seq fragments spanning BSJ sites,which is different from the quantification of linear RNA expression by normalized RNA-seq fragments mapped to whole gene bodies.Thus,direct comparison of circular and linear RNA expression from the same gene loci in a genome-wide manner has remained challenging.Here,we update the previously-reported CIRCexplorer pipeline to version 3 for circular and linear RNA expression analysis from ribosomal-RNA depleted RNA-seq(CIRCexplorer3-CLEAR).A new quantitation parameter,fragments per billion mapped bases(FPB),is applied to evaluate circular and linear RNA expression individually by fragments mapped to circ RNA-specific BSJ sites or to linear RNA-specific splicing junction(SJ)sites.Comparison of circular and linear RNA expression levels is directly achieved by dividing FPBcircby FPBlinearto generate a CIRCscore,which indicates the relative circ RNA expression level using linear RNA expression level as the background.Highlyexpressed circ RNAs with low cognate linear RNA expression background can be readily identified by CIRCexplorer3-CLEAR for further investigation.CIRCexplorer3-CLEAR is publically available at https://github.com/Yang Lab/CLEAR.
