AIM:To evaluate the effect of posterior sclera collagen cross-linking induced by riboflavin-ultraviolet A(UVA)on form-deprived myopia in guinea pigs.METHODES:Twenty-five pigmented guinea pigs of 3-week-old were random...AIM:To evaluate the effect of posterior sclera collagen cross-linking induced by riboflavin-ultraviolet A(UVA)on form-deprived myopia in guinea pigs.METHODES:Twenty-five pigmented guinea pigs of 3-week-old were randomly assigned into 4 groups that included normal control(NOR,n=7),form-deprived(FDM,n=7),normal with riboflavin-UVA cross-linking(NOR+CL,n=5)and form-deprived with cross-linking(FDM+CL,n=6).The NOR+CL group and the FDM+CL group received the riboflavin-UVA induced cross-linking at day 0.FDM was induced by monocularly deprived with facemask in the right eyes.The refraction,axial length and corneal curvature were measured by retinoscopy,A-scan and keratometer respectively in scheduled time points(day 0 and 1,2,3,4 wk after form-deprivation).At the end of 4 weeks’experiment,stress-strain tests of sclera were measured and morphological changes of sclera and retina were examined.RESULTS:After 4 wk,the interocular difference of refractive error were-0.11±0.67,-2.93±0.56,1.10±0.58,and-1.63±0.41 D in the NOR,FDM,NOR+CL,and FDM+CL groups respectively.Mixed-effect linear model revealed significant effect of FDM(P<0.01)and CL(P<0.001).Also,after 4 wk,the interocular difference of axial length were 0.01±0.04,0.29±0.07,-0.13±0.06,and 0.11±0.05 mm in the NOR,FDM,NOR+CL,and FDM+CL group.Mixedeffect linear model revealed significant effect of FDM(P<0.001)and CL(P<0.01).As for corneal curvature,significant interocular difference have not found between any of the two groups.At the end of this experiment,the ultimate stress and elastic modulus were found significantly increased in both CL groups.But no difference was found in the groups without cross-linked.There was no abnormality observed in the retina and RPE cells of the treated eyes.CONCLUSION:The posterior sclera collagen crosslinking induced by riboflavin-UVA can slow down the progress of myopia and increase the sclera biomechanical strength in the guinea pig model of form-deprived myopia.展开更多
目的：评价角膜厚度低于400μm 的圆锥角膜患者行核黄素诱导的长波紫外线角膜交联术的安全性和有效性。方法选择角膜厚度低于400μm 的圆锥角膜患者14例18眼，表面麻醉下去除上皮，点低渗核黄素溶液使角膜基质水肿厚度达到400μm 以上...目的：评价角膜厚度低于400μm 的圆锥角膜患者行核黄素诱导的长波紫外线角膜交联术的安全性和有效性。方法选择角膜厚度低于400μm 的圆锥角膜患者14例18眼，表面麻醉下去除上皮，点低渗核黄素溶液使角膜基质水肿厚度达到400μm 以上，行角膜交联术，术后戴软性角膜接触镜。术前、术后记录观察最佳矫正视力、角膜顶点 K 值、角膜厚度、角膜内皮、晶体等变化。结果术后所有患者角膜上皮于1周内愈合，14眼于5 d 内愈合；角膜 Haze 于2～6周内消退；最佳矫正视力：术前为4．76±0．61，术后3个月为4．83±0．46，6个月为4．84±0．51；角膜顶点 K 值：术前为（56．29±7．33）D，3个月为（52．42±5．76）D，6个月为（48．12±6．6）D；角膜厚度：治疗前为（318±33）μm，水肿后为（447±18）μm，3个月为（381±72）μm，6个月为（354±35）μm；角膜内皮密度：术前为（3069±202）／mm2，术后3个月为（2910±213）／mm2，6个月为（2935±215）／mm2；未见晶体、视网膜并发症。结论采用低渗核黄素法行角膜交联术治疗晚期薄角膜圆锥安全、有效。展开更多
基金Supported by the Tianjin Clinical Key Discipline Project(No.TJLCZDXKQ013)the Research Project of Health Committee in Binhai District,Tianjin(No.2019BWKQ033)。
文摘AIM:To evaluate the effect of posterior sclera collagen cross-linking induced by riboflavin-ultraviolet A(UVA)on form-deprived myopia in guinea pigs.METHODES:Twenty-five pigmented guinea pigs of 3-week-old were randomly assigned into 4 groups that included normal control(NOR,n=7),form-deprived(FDM,n=7),normal with riboflavin-UVA cross-linking(NOR+CL,n=5)and form-deprived with cross-linking(FDM+CL,n=6).The NOR+CL group and the FDM+CL group received the riboflavin-UVA induced cross-linking at day 0.FDM was induced by monocularly deprived with facemask in the right eyes.The refraction,axial length and corneal curvature were measured by retinoscopy,A-scan and keratometer respectively in scheduled time points(day 0 and 1,2,3,4 wk after form-deprivation).At the end of 4 weeks’experiment,stress-strain tests of sclera were measured and morphological changes of sclera and retina were examined.RESULTS:After 4 wk,the interocular difference of refractive error were-0.11±0.67,-2.93±0.56,1.10±0.58,and-1.63±0.41 D in the NOR,FDM,NOR+CL,and FDM+CL groups respectively.Mixed-effect linear model revealed significant effect of FDM(P<0.01)and CL(P<0.001).Also,after 4 wk,the interocular difference of axial length were 0.01±0.04,0.29±0.07,-0.13±0.06,and 0.11±0.05 mm in the NOR,FDM,NOR+CL,and FDM+CL group.Mixedeffect linear model revealed significant effect of FDM(P<0.001)and CL(P<0.01).As for corneal curvature,significant interocular difference have not found between any of the two groups.At the end of this experiment,the ultimate stress and elastic modulus were found significantly increased in both CL groups.But no difference was found in the groups without cross-linked.There was no abnormality observed in the retina and RPE cells of the treated eyes.CONCLUSION:The posterior sclera collagen crosslinking induced by riboflavin-UVA can slow down the progress of myopia and increase the sclera biomechanical strength in the guinea pig model of form-deprived myopia.
文摘目的：评价角膜厚度低于400μm 的圆锥角膜患者行核黄素诱导的长波紫外线角膜交联术的安全性和有效性。方法选择角膜厚度低于400μm 的圆锥角膜患者14例18眼，表面麻醉下去除上皮，点低渗核黄素溶液使角膜基质水肿厚度达到400μm 以上，行角膜交联术，术后戴软性角膜接触镜。术前、术后记录观察最佳矫正视力、角膜顶点 K 值、角膜厚度、角膜内皮、晶体等变化。结果术后所有患者角膜上皮于1周内愈合，14眼于5 d 内愈合；角膜 Haze 于2～6周内消退；最佳矫正视力：术前为4．76±0．61，术后3个月为4．83±0．46，6个月为4．84±0．51；角膜顶点 K 值：术前为（56．29±7．33）D，3个月为（52．42±5．76）D，6个月为（48．12±6．6）D；角膜厚度：治疗前为（318±33）μm，水肿后为（447±18）μm，3个月为（381±72）μm，6个月为（354±35）μm；角膜内皮密度：术前为（3069±202）／mm2，术后3个月为（2910±213）／mm2，6个月为（2935±215）／mm2；未见晶体、视网膜并发症。结论采用低渗核黄素法行角膜交联术治疗晚期薄角膜圆锥安全、有效。
