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Analysis of Short-Term Metabolic Alterations in Arabidopsis Following Changes in the Prevailing Environmental Conditions 被引量:2
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作者 Alexandra Florian Zoran Nikoloski +5 位作者 Ronan Sulpice Stefan Timm Wagner L. Araujo Takayuki Tohge Hermann Bauwe Alisdair R. Fernie 《Molecular Plant》 SCIE CAS CSCD 2014年第5期893-911,共19页
Although a considerable increase in our knowledge concerning the importance of metabolic adjustments to unfavorable growth conditions has been recently provided, relatively little is known about the adjustments which ... Although a considerable increase in our knowledge concerning the importance of metabolic adjustments to unfavorable growth conditions has been recently provided, relatively little is known about the adjustments which occur in response to fluctuation in environmental factors. Evaluating the metabolic adjustments occurring under changing environmental conditions thus offers a good opportunity to increase our current understanding of the crosstalk between the major pathways which are affected by such conditions. To this end, plants growing under normal conditions were transferred to different light and temperature conditions which were anticipated to affect (amongst other processes) the rates of photosynthesis and photorespiration and characterized at the physiological, molecular, and metabolic levels following this transition. Our results revealed similar behavior in response to both treatments and imply a tight connec- tivity of photorespiration with the major pathways of plant metabolism. They further highlight that the majority of the regulation of these pathways is not mediated at the level of transcription but that leaf metabolism is rather pre-poised to adapt to changes in these input parameters. 展开更多
关键词 central metabolism environmental perturbation gene expression metabolic regulation PHOTORESPIRATION PHOTOSYNTHESIS ribulose-1 5-bisphosphate carboxylase/oxygenase rubisco).
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复苏植物垫状卷柏离体叶片光合作用对脱水和复水的响应 被引量:2
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作者 余蓉培 汪国鲜 +7 位作者 李帆 单芹丽 吴丽芳 阮继伟 王继华 吴旻 杨春梅 屈云慧 《植物生理学报》 CAS CSCD 北大核心 2019年第12期1806-1816,共11页
本研究对复苏植物垫状卷柏离体叶片进行脱水和复水处理,研究叶绿素含量、叶绿素荧光以及核酮糖-1,5-二磷酸羧化酶/加氧酶(Rubisco)总活性变化,旨在探究垫状卷柏光合作用对脱水和复水的响应。研究结果显示,垫状卷柏离体叶片叶绿素总含量... 本研究对复苏植物垫状卷柏离体叶片进行脱水和复水处理,研究叶绿素含量、叶绿素荧光以及核酮糖-1,5-二磷酸羧化酶/加氧酶(Rubisco)总活性变化,旨在探究垫状卷柏光合作用对脱水和复水的响应。研究结果显示,垫状卷柏离体叶片叶绿素总含量在脱水和复水过程中没有发生显著变化,符合叶绿素保持型复苏植物的特征。PSII最大光化学效率(Fv/Fm)、非光化学淬灭(NPQ)、PSII有效光化学量子产量(Fv’/Fm’)以及PSII实际光化学量子效率(ΦPSII)随脱水时间的延长而降低,随复水时间的延长而上升;复水24 h时,各参数值趋近正常叶片数值。脱水和复水过程中的光合电子传递速率(ETR)快速光响应曲线与光强的变化趋势相似,但ETR数值在脱水时下降,复水时恢复。脱水过程中Rubisco总活性降低,复水时活性逐渐增加。上述结果表明垫状卷柏离体叶片光合作用在脱水过程中受到抑制,复水时逐步恢复。垫状卷柏叶片对脱水和复水的快速响应是应对脱水胁迫的重要策略。 展开更多
关键词 垫状卷柏 复苏植物 耐脱水性 叶绿素荧光 核酮糖-1 5-二磷酸羧化酶/加氧酶(rubisco)
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