Objective:To determine distribution of arthropods in processed rice products such as rice flour and rice cereal-based infant food.Methods:Random samples of rice flour and rice cereal-based infant food purchased from c...Objective:To determine distribution of arthropods in processed rice products such as rice flour and rice cereal-based infant food.Methods:Random samples of rice flour and rice cereal-based infant food purchased from commercial outlets were examined for the presence of arthropods using a modified Berlese Tullgren Funnel Method.Mites were mounted prior to identification and weevils were directly identified.Results:For non-expired products, infestation was found in 6.7%of rice flour and none was found in rice cereal-based infant food samples.The arthropods found in the flour samples were Cheyletus spp.,Suidasia pontifica(S. pontifica),Tarsonemus spp.,Tyrophagus putrescentiae(T.putrescentiae),Sitophilus granarius(S. granarius) and Sitophilus oryzae(S.oryzae).Others which cannot be identified were Oribatid and Prostigmatid mites.The most common mites in rice flour were Tarsonemus spp.(69.1%),followed by S.pontifica(18.2%).For expired products,only one sample of rice cereal-based infant food was infested and the infestation was by mites of the family Tydeidae.Conclusions:This study demonstrates the presence of 4 allergenic species of S.pontifica,T.putrescentiae,S.granarius and S.oryzae in rice flour.These arthropods can contribute to the incidence of anaphylaxis upon consumption by atopic individuals.There was no infestation of arthropods in rice cereal-based infant food surveyed except for an expired product in a moderate rusty tin container.展开更多
In the present study, we report a survey on a Miniature Inverted Transposable Element （MITE） system known as mPing in 102 varieties of Asian cultivated rice （Oryza sativa L.）. We found that mPing populations could...In the present study, we report a survey on a Miniature Inverted Transposable Element （MITE） system known as mPing in 102 varieties of Asian cultivated rice （Oryza sativa L.）. We found that mPing populations could be generalized Into two families, mPing-1 and mPing-2, according to their sequence structures. Further analysis showed that these two families of mPing had significant bias in their distribution pattern in two subspecies of rice, namely O. sativa ssp. japonica and indica. 0. sativa japonica has a higher proportion of mPing-1 as a general trait, whereas 0. sativa indica has a higher proportion of roPing-2. We also examined the mPing system In a doubled haploid （DH） cross-breeding population of jingxi 17 （japonica） and zhaiyeqing 8 （indica） varieties and observed that the mPing system was not tightly linked to major subspecies-determining genes. Furthermore, we checked the mPing system in 28 accessions of Asian common wild rice O. rufipogon and found the roPing system in 0. rufipogon. The distribution pattern of the roPing system in O. rufipogon indicated a diphyletlc origin of the Asian cultivated rice O. sativa species. We did not find the mPing system in another 20 Oryza species. These results substantiated a previous hypothesis that O. ruflpogon and O. nivara species were the closest relatives of O. sativa and that the two extant subspecies of O. sativa were evolved independently from corresponding ecotypes of O. ruflpogon.展开更多
文摘Objective:To determine distribution of arthropods in processed rice products such as rice flour and rice cereal-based infant food.Methods:Random samples of rice flour and rice cereal-based infant food purchased from commercial outlets were examined for the presence of arthropods using a modified Berlese Tullgren Funnel Method.Mites were mounted prior to identification and weevils were directly identified.Results:For non-expired products, infestation was found in 6.7%of rice flour and none was found in rice cereal-based infant food samples.The arthropods found in the flour samples were Cheyletus spp.,Suidasia pontifica(S. pontifica),Tarsonemus spp.,Tyrophagus putrescentiae(T.putrescentiae),Sitophilus granarius(S. granarius) and Sitophilus oryzae(S.oryzae).Others which cannot be identified were Oribatid and Prostigmatid mites.The most common mites in rice flour were Tarsonemus spp.(69.1%),followed by S.pontifica(18.2%).For expired products,only one sample of rice cereal-based infant food was infested and the infestation was by mites of the family Tydeidae.Conclusions:This study demonstrates the presence of 4 allergenic species of S.pontifica,T.putrescentiae,S.granarius and S.oryzae in rice flour.These arthropods can contribute to the incidence of anaphylaxis upon consumption by atopic individuals.There was no infestation of arthropods in rice cereal-based infant food surveyed except for an expired product in a moderate rusty tin container.
基金Supported by Grants from the Ministry of Science and Technology of China （2002AA2Z1003 and 2003AA222091 ）, Shanghai Municipal Commission of Science and Technology （038019315）, and the National Natural Science Foundation of China （30325014）.
文摘In the present study, we report a survey on a Miniature Inverted Transposable Element （MITE） system known as mPing in 102 varieties of Asian cultivated rice （Oryza sativa L.）. We found that mPing populations could be generalized Into two families, mPing-1 and mPing-2, according to their sequence structures. Further analysis showed that these two families of mPing had significant bias in their distribution pattern in two subspecies of rice, namely O. sativa ssp. japonica and indica. 0. sativa japonica has a higher proportion of mPing-1 as a general trait, whereas 0. sativa indica has a higher proportion of roPing-2. We also examined the mPing system In a doubled haploid （DH） cross-breeding population of jingxi 17 （japonica） and zhaiyeqing 8 （indica） varieties and observed that the mPing system was not tightly linked to major subspecies-determining genes. Furthermore, we checked the mPing system in 28 accessions of Asian common wild rice O. rufipogon and found the roPing system in 0. rufipogon. The distribution pattern of the roPing system in O. rufipogon indicated a diphyletlc origin of the Asian cultivated rice O. sativa species. We did not find the mPing system in another 20 Oryza species. These results substantiated a previous hypothesis that O. ruflpogon and O. nivara species were the closest relatives of O. sativa and that the two extant subspecies of O. sativa were evolved independently from corresponding ecotypes of O. ruflpogon.
基金This research was supported by the Specialized Research Fund for the Doctoral Program of Higher Education (SRFDP) from the Ministry of Education of China (No. B200211)
