The aim of the experiment was to explore the feasibility of discarded nutrient medium of Cordyceps militaris as feed in the production of laying hens. 100 g/kg, 200 g/kg, 300 g/kg of discarded nutrient medium of Cordy...The aim of the experiment was to explore the feasibility of discarded nutrient medium of Cordyceps militaris as feed in the production of laying hens. 100 g/kg, 200 g/kg, 300 g/kg of discarded nutrient medium of Cordyceps militaris were added to the basal diet of laying hens. The results showed that the optimal addition of discarded nutrient medium of Cordyceps militaris in the diet of laying hens was 10%. According to the results of measuring the conventional indicators of eggs, the weight of eggs produced by laying hens fed with discarded nutrient medium of Cordyceps militaris was higher than that of laying hens fed with ordinary laying hens. The content of interleukin-1(IL-1) in the experimental group was significantly higher than that in the control group, and the concentration of IL-1 increased by 141.5 pg/mL, which indicated that the application of discarded nutrient medium of Cordyceps militaris effectively improved the immunity of laying hens. The high-throughput analysis of the intestinal contents of the two groups of laying hens showed that the microbial population abundance of the intestinal tract of the experimental group was greater than that of control group, and the application of discarded nutrient medium of Cordyceps militaris increased the diversity of bacteria in the intestinal tract of laying hens. In addition, the sensitivity of some pathogenic bacteria in the intestinal tract of chickens to drugs was also increased, thereby reducing the use of antibiotics. The secondary utilization of discarded nutrient medium of Cordyceps militaris has great development and utilization prospects, which provided a scientific reference and basic theoretical basis for the development of discarded nutrient medium of Cordyceps militaris as feed in the production of laying hens.展开更多
Aim: To compare the contents of nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia, and to study the effect of humidity and heat on the content of nucleosides. Methods: The contents of nucleos...Aim: To compare the contents of nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia, and to study the effect of humidity and heat on the content of nucleosides. Methods: The contents of nucleosides were determined by using high performance capillary electrophoresis (HPCE). Beckman P/ACE System 5010 apparatus equipped with a UV detector and a Beckman untreated fused-silica capillary (57 cm 75 mm, 50 cm effective length) was used. Before sample injection, the capillary was rinsed with 1 molL-1 sodium hydroxide solution and running buffer for 5 min, respectively. A voltage of 20 kV was applied for the separation. Pressure injection was 586 kPa for 6 seconds, and the wavelength of detector was 254 nm. The running time was 20 min at 20 oC. The effect of humidity and heat on the contents of nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia was observed for 1, 3, 5 and 10 days at temperature 40 oC, and relative humidity 75%. Results: The content of nucleosides from natural Cordyceps sinensis was higher than that from cultured Cordyceps mycelia. But the contents of nucleosides from freshly collected natural Cordyceps sinensis were very low, even below the limit of quantitation. The contents of nucleosides from natural Cordyceps sinensis were significantly increased by humidity and heat, but this phenomenon was not observed in cultured Cordyceps mycelia. Conclusion: There are differences between the nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia. The nucleosides in natural Cordyceps sinensis may be derived from the degradation of nucleic acids. This implies that adenosine being used for the quality control of natural Cordyceps sinensis may have to be reconsidered.展开更多
利用离子束、亚硝基胍及离子束-亚硝基胍诱变法处理蛹虫草Cordyceps militaris JN168,初步获得了几株较高产虫草素的菌株。通过进一步筛选得到复合诱变菌2,并对该菌的液态发酵培养基组分进行了优化。实验结果表明:蛹虫草液态发酵产虫草...利用离子束、亚硝基胍及离子束-亚硝基胍诱变法处理蛹虫草Cordyceps militaris JN168,初步获得了几株较高产虫草素的菌株。通过进一步筛选得到复合诱变菌2,并对该菌的液态发酵培养基组分进行了优化。实验结果表明:蛹虫草液态发酵产虫草素的最佳培养基组分为(g/L),葡萄糖40,酵母浸粉25,Mg SO4·7H2O 0.6,K2HPO4·3H2O 0.6,KH2PO40.6。优化后虫草素产量提高了5倍,最高达1 045.65 mg/L。展开更多
以一株生长性状优良的Cordyceps militaris FFCC5111为出发菌株,通过复合诱变,筛选高产Cordycepin双重营养缺陷型(Xan-+Gua-)菌株。采用200 W、22kHz超声强度联合1%DES复合诱变,获得双重营养缺陷型突变株7株,经抗菌试验、摇瓶培养/发酵...以一株生长性状优良的Cordyceps militaris FFCC5111为出发菌株,通过复合诱变,筛选高产Cordycepin双重营养缺陷型(Xan-+Gua-)菌株。采用200 W、22kHz超声强度联合1%DES复合诱变,获得双重营养缺陷型突变株7株,经抗菌试验、摇瓶培养/发酵复筛至第16天,突变株C.militaris FFCC5111-c产生的Cordycepin总量为4.142g/L,比亲株提高44.5%。试验证明,筛选Xanthine和Guanine双重营养缺陷型突变株是获得Cordycepin高产菌种的有效方法,Cordycepin的生物合成与Guanine代谢相关。展开更多
基金Project supported by Key Project of Education Department of Liaoning Province(LJKZZ20220116)Surface Project of Science and Technology Department of Liaoning Province(2023-MS-251).
文摘The aim of the experiment was to explore the feasibility of discarded nutrient medium of Cordyceps militaris as feed in the production of laying hens. 100 g/kg, 200 g/kg, 300 g/kg of discarded nutrient medium of Cordyceps militaris were added to the basal diet of laying hens. The results showed that the optimal addition of discarded nutrient medium of Cordyceps militaris in the diet of laying hens was 10%. According to the results of measuring the conventional indicators of eggs, the weight of eggs produced by laying hens fed with discarded nutrient medium of Cordyceps militaris was higher than that of laying hens fed with ordinary laying hens. The content of interleukin-1(IL-1) in the experimental group was significantly higher than that in the control group, and the concentration of IL-1 increased by 141.5 pg/mL, which indicated that the application of discarded nutrient medium of Cordyceps militaris effectively improved the immunity of laying hens. The high-throughput analysis of the intestinal contents of the two groups of laying hens showed that the microbial population abundance of the intestinal tract of the experimental group was greater than that of control group, and the application of discarded nutrient medium of Cordyceps militaris increased the diversity of bacteria in the intestinal tract of laying hens. In addition, the sensitivity of some pathogenic bacteria in the intestinal tract of chickens to drugs was also increased, thereby reducing the use of antibiotics. The secondary utilization of discarded nutrient medium of Cordyceps militaris has great development and utilization prospects, which provided a scientific reference and basic theoretical basis for the development of discarded nutrient medium of Cordyceps militaris as feed in the production of laying hens.
文摘Aim: To compare the contents of nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia, and to study the effect of humidity and heat on the content of nucleosides. Methods: The contents of nucleosides were determined by using high performance capillary electrophoresis (HPCE). Beckman P/ACE System 5010 apparatus equipped with a UV detector and a Beckman untreated fused-silica capillary (57 cm 75 mm, 50 cm effective length) was used. Before sample injection, the capillary was rinsed with 1 molL-1 sodium hydroxide solution and running buffer for 5 min, respectively. A voltage of 20 kV was applied for the separation. Pressure injection was 586 kPa for 6 seconds, and the wavelength of detector was 254 nm. The running time was 20 min at 20 oC. The effect of humidity and heat on the contents of nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia was observed for 1, 3, 5 and 10 days at temperature 40 oC, and relative humidity 75%. Results: The content of nucleosides from natural Cordyceps sinensis was higher than that from cultured Cordyceps mycelia. But the contents of nucleosides from freshly collected natural Cordyceps sinensis were very low, even below the limit of quantitation. The contents of nucleosides from natural Cordyceps sinensis were significantly increased by humidity and heat, but this phenomenon was not observed in cultured Cordyceps mycelia. Conclusion: There are differences between the nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia. The nucleosides in natural Cordyceps sinensis may be derived from the degradation of nucleic acids. This implies that adenosine being used for the quality control of natural Cordyceps sinensis may have to be reconsidered.
文摘以一株生长性状优良的Cordyceps militaris FFCC5111为出发菌株,通过复合诱变,筛选高产Cordycepin双重营养缺陷型(Xan-+Gua-)菌株。采用200 W、22kHz超声强度联合1%DES复合诱变,获得双重营养缺陷型突变株7株,经抗菌试验、摇瓶培养/发酵复筛至第16天,突变株C.militaris FFCC5111-c产生的Cordycepin总量为4.142g/L,比亲株提高44.5%。试验证明,筛选Xanthine和Guanine双重营养缺陷型突变株是获得Cordycepin高产菌种的有效方法,Cordycepin的生物合成与Guanine代谢相关。
