Background: Data on the serum vitamin D3 levels of vernal keratoconjunctivitis (VKC) children are not known well in Central Africa. Children with vernal keratoconjunctivitis are less exposed to sunlight. There is no s...Background: Data on the serum vitamin D3 levels of vernal keratoconjunctivitis (VKC) children are not known well in Central Africa. Children with vernal keratoconjunctivitis are less exposed to sunlight. There is no study that shows the breadth of the serum vitamin D3 levels of vernal keratoconjunctivitis children and normal in our setting. Purpose: This study aimed to compare the serum 25-hydroxy vitamin D [25(OH)D3] levels of children with and without VKC. Methods: An observational, case-control study was performed from September 2019 to February 2020 in the Ophthalmology Department and the Clinical Biology Department of the University Hospital of Kinshasa. The case group was recruited consecutively in the consultation while the control group was enrolled in the nearest Rehoboth school complex using a systematic sample method (k = 4) on the presence register. Both cases and controls underwent the routine ophthalmological examination of the anterior segment and serum vitamin D3 levels were measured in both cases and controls using Mindray Chimic-Luminescence (CL)-1200i. Data were entered in Microsoft Sheets and analyzed using SPSS version 20.0. The Mann-Whitney-U test (M-U), and the t-Student test were used respectively to compare the mean serum vitamin D3 levels in both cases and controls. The correlation of Spearman (rho) was used to determine the association between Body Mass Index and serum vitamin D3 levels in the case group. Results: A total of 75 children with VKC (mean age 7.8 ± 4.4 years) and 75 children without VKC non-atopic control group (mean age 7.9 ± 4.3 years) were enrolled in this study. The vitamin D3 deficiency was more frequent among the case group than in the control group (40% vs 8%, p = 0.0001). Vitamin D3 insufficiency was more frequent in the case group than in the control group (33.3% vs 18.6%, p = 0.04). The mean serum vitamin 25(OH)D3 levels were statistically lower in children with VKC compared to those without (25.5 ± 8.7 ng/ml and 44.3 ± 18.5 ng/ml, p = 0.0001). Time spent outdoors during daylight by children with VKC was statistically lower than children without VKC (1.59 ± 0.71 hours and 2.28 ± 1.08 hours, respectively) (p = 0.0001). Body Mass Index and serum vitamin D3 levels in VKC children showed a negative correlation statistically significant (Spearman, rho = -0.452, p = 0.0001). Conclusion: This study showed statistically values of serum vitamin D3 levels in VKC children lower than in children without VKC.展开更多
Background: Hypovitaminosis D is reported through the literature to be involved in autoimmune diseases such as multiple sclerosis (MS). In the last decade, numerous studies have investigated the association of single ...Background: Hypovitaminosis D is reported through the literature to be involved in autoimmune diseases such as multiple sclerosis (MS). In the last decade, numerous studies have investigated the association of single nucleotide polymorphisms (SNPs) with MS, including rs2248359 (CYP24A1) and rs703842 (CYP27B1) that are involved in vitamin D metabolic pathway. However, results were conflicting, probably due to ethnic differences between the studied populations. In this context, the present study aimed to analyze the association between these two SNPs and MS within the Moroccan population. Methods: rs2248359 and rs703842 were genotyped in 113 patients and 146 healthy controls. To assess their association with the disease risk, we compared the genotypic and allelic frequencies between the study groups. We also explored their possible influence on certain clinical features (age at onset, type, disability status and severity score) and with vitamin D3 serum level (DSL) by comparing mean values of these variables between the different genotypes. Results: No statistically significant differences in the distribution of both SNPs were found between patients and controls. A trend has emerged concerning the minor G allele of rs703842 which appears to have a protective effect on developing MS, but this result remained slightly below significance. Also, the two polymorphisms had no impact on the clinical features tested and the DSL. Conclusion: There is no convincing evidence that rs2248359 and rs703842 are associated with MS risk, its clinical features or vitamin D level in Moroccans. Further larger investigations are needed to confirm these findings.展开更多
文摘Background: Data on the serum vitamin D3 levels of vernal keratoconjunctivitis (VKC) children are not known well in Central Africa. Children with vernal keratoconjunctivitis are less exposed to sunlight. There is no study that shows the breadth of the serum vitamin D3 levels of vernal keratoconjunctivitis children and normal in our setting. Purpose: This study aimed to compare the serum 25-hydroxy vitamin D [25(OH)D3] levels of children with and without VKC. Methods: An observational, case-control study was performed from September 2019 to February 2020 in the Ophthalmology Department and the Clinical Biology Department of the University Hospital of Kinshasa. The case group was recruited consecutively in the consultation while the control group was enrolled in the nearest Rehoboth school complex using a systematic sample method (k = 4) on the presence register. Both cases and controls underwent the routine ophthalmological examination of the anterior segment and serum vitamin D3 levels were measured in both cases and controls using Mindray Chimic-Luminescence (CL)-1200i. Data were entered in Microsoft Sheets and analyzed using SPSS version 20.0. The Mann-Whitney-U test (M-U), and the t-Student test were used respectively to compare the mean serum vitamin D3 levels in both cases and controls. The correlation of Spearman (rho) was used to determine the association between Body Mass Index and serum vitamin D3 levels in the case group. Results: A total of 75 children with VKC (mean age 7.8 ± 4.4 years) and 75 children without VKC non-atopic control group (mean age 7.9 ± 4.3 years) were enrolled in this study. The vitamin D3 deficiency was more frequent among the case group than in the control group (40% vs 8%, p = 0.0001). Vitamin D3 insufficiency was more frequent in the case group than in the control group (33.3% vs 18.6%, p = 0.04). The mean serum vitamin 25(OH)D3 levels were statistically lower in children with VKC compared to those without (25.5 ± 8.7 ng/ml and 44.3 ± 18.5 ng/ml, p = 0.0001). Time spent outdoors during daylight by children with VKC was statistically lower than children without VKC (1.59 ± 0.71 hours and 2.28 ± 1.08 hours, respectively) (p = 0.0001). Body Mass Index and serum vitamin D3 levels in VKC children showed a negative correlation statistically significant (Spearman, rho = -0.452, p = 0.0001). Conclusion: This study showed statistically values of serum vitamin D3 levels in VKC children lower than in children without VKC.
文摘Background: Hypovitaminosis D is reported through the literature to be involved in autoimmune diseases such as multiple sclerosis (MS). In the last decade, numerous studies have investigated the association of single nucleotide polymorphisms (SNPs) with MS, including rs2248359 (CYP24A1) and rs703842 (CYP27B1) that are involved in vitamin D metabolic pathway. However, results were conflicting, probably due to ethnic differences between the studied populations. In this context, the present study aimed to analyze the association between these two SNPs and MS within the Moroccan population. Methods: rs2248359 and rs703842 were genotyped in 113 patients and 146 healthy controls. To assess their association with the disease risk, we compared the genotypic and allelic frequencies between the study groups. We also explored their possible influence on certain clinical features (age at onset, type, disability status and severity score) and with vitamin D3 serum level (DSL) by comparing mean values of these variables between the different genotypes. Results: No statistically significant differences in the distribution of both SNPs were found between patients and controls. A trend has emerged concerning the minor G allele of rs703842 which appears to have a protective effect on developing MS, but this result remained slightly below significance. Also, the two polymorphisms had no impact on the clinical features tested and the DSL. Conclusion: There is no convincing evidence that rs2248359 and rs703842 are associated with MS risk, its clinical features or vitamin D level in Moroccans. Further larger investigations are needed to confirm these findings.
