为查明天津地区养殖红尾皇冠鱼(Aequidens rivulatus)大量死亡的原因,取患病红尾皇冠鱼进行细菌分离,从病鱼肾脏中分离到优势菌株071901,人工感染试验证实其对红尾皇冠鱼有较强的致病性;采用形态学观察、生理生化特性、16S r RNA基因序...为查明天津地区养殖红尾皇冠鱼(Aequidens rivulatus)大量死亡的原因,取患病红尾皇冠鱼进行细菌分离,从病鱼肾脏中分离到优势菌株071901,人工感染试验证实其对红尾皇冠鱼有较强的致病性;采用形态学观察、生理生化特性、16S r RNA基因序列系统发育树分析及cfb(GBS-specific gene cfb,CAMP factor)基因检测对菌株071901进行鉴定。结果显示,菌株071901为革兰氏阳性球菌,生化特性与链球菌属的无乳链球菌(Streptococcus agalactiae)相近,基于16S r RNA基因序列的系统发育分析将菌株071901与无乳链球菌聚为一支,以071901为模板特异性扩增无乳链球菌的cfb基因,也得到了预期的900 bp的核酸片段,进一步证实菌株071901为无乳链球菌。对30种抗生素的药敏结果显示,菌株071901对红霉素、阿奇霉素等19种药物敏感,对多粘霉素、罗红霉素、呋喃唑酮等11种药物耐药。展开更多
以cfb基因保守序列的6个区域设计4条特异性引物,利用链置换DNA聚合酶（Bst DNA polymerase）在恒温（65℃）下保温40 min,建立了无乳链球菌Streptococcus agalactiae的LAMP技术。建立的LAMP方法能够特异性地检测无乳链球菌,检测灵敏度为...以cfb基因保守序列的6个区域设计4条特异性引物,利用链置换DNA聚合酶（Bst DNA polymerase）在恒温（65℃）下保温40 min,建立了无乳链球菌Streptococcus agalactiae的LAMP技术。建立的LAMP方法能够特异性地检测无乳链球菌,检测灵敏度为3.7×10^1～3.7×10^2cfu/mL,反应前加入显色剂钙黄绿素避免二次污染。现场应用中采用FTA卡采集红尾皇冠鱼Aequidens rivulatus病鱼肝脏组织病原菌,操作简便、快捷。研究表明,针对无乳链球菌cfb基因建立的LAMP检测方法具有较高的特异性及稳定性,尤其是具有快速、简便、成本低等优点,可用于无乳链球菌的野外现场快速检测。展开更多
Ponyfishes of the family Leiognathidae frequently show overlapping distribution pattems in the Indo-Pacific and South China Sea. The mouth morphology of three species of ponyfishes which feed at different lerels in th...Ponyfishes of the family Leiognathidae frequently show overlapping distribution pattems in the Indo-Pacific and South China Sea. The mouth morphology of three species of ponyfishes which feed at different lerels in the water column were compared. The species could be distinguished primarily by the mouth meropology. Gazza minuta (Bloch)feeds mainly on bethos, has the least protruding jaws; Secutor ruconius (Hamilton-Buchanan) feeds mainly on plankton, has the most protruding jaws, Leiognathus rivuatus (Temmink et Schlegel) has an intermediate deggree of jaw protrusion. This study showed that closely related species with overlapping distributions frequently exhibit morphologlcal, phpsiological or ecological differences related to the search for food, the intake of food or locomotion mode.展开更多
文摘Ponyfishes of the family Leiognathidae frequently show overlapping distribution pattems in the Indo-Pacific and South China Sea. The mouth morphology of three species of ponyfishes which feed at different lerels in the water column were compared. The species could be distinguished primarily by the mouth meropology. Gazza minuta (Bloch)feeds mainly on bethos, has the least protruding jaws; Secutor ruconius (Hamilton-Buchanan) feeds mainly on plankton, has the most protruding jaws, Leiognathus rivuatus (Temmink et Schlegel) has an intermediate deggree of jaw protrusion. This study showed that closely related species with overlapping distributions frequently exhibit morphologlcal, phpsiological or ecological differences related to the search for food, the intake of food or locomotion mode.
