[Objective] This study aimed to establish a tissue culture technology for Leucophyta brownii ‘Canal Rocks Form’. [Method] Leucophyta brownii ‘Canal Rocks Form’ stems with axillary buds were selected as explants in...[Objective] This study aimed to establish a tissue culture technology for Leucophyta brownii ‘Canal Rocks Form’. [Method] Leucophyta brownii ‘Canal Rocks Form’ stems with axillary buds were selected as explants in vitro to investigate the effects of different concentrations of 6-BA and NAA on proliferation culture and the effects of IBA, NAA and subculture cycle on rooting culture. [Result] The results showed that Leucophyta brownii ‘Canal Rocks Form’ plantlets need low concentrations of phytohormones and the rooting culture was significantly affected by the subculture cycle; the optimal medium for proliferation culture was MS＋0.5 mg/L 6-BA＋0.05 mg/L NAA＋30 g/L sucrose＋5.5 g/L agar, with a proliferation coefficient of 4.25; the plantlets with a subculture cycle of 28 d were the most suitable for rooting culture, with a rooting rate of 95.9% in 1/2 MS＋0.1 mg/L IBA＋0.05 mg/L NAA＋20 g/L sucrose＋5.5 g/L agar, and the average number and length of roots were 4.69 and 1.68 cm, respectively. [Conclusion] This study laid the foundation for establishing sterile culture system of Leucophyta brownii ‘Canal Rocks Form’.展开更多
The characteristics of the flow field associated with a multi-hole combined external rotary bit have been studied by means of numerical simulation in the framework of an RNG k-εturbulence model,and compared with the ...The characteristics of the flow field associated with a multi-hole combined external rotary bit have been studied by means of numerical simulation in the framework of an RNG k-εturbulence model,and compared with the results of dedicated rock breaking drilling experiments.The numerical results show that the nozzle velocity and dynamic pressure of the nozzle decrease with an increase in the jet distance,and the axial velocity of the nozzle decays regularly with an increase in the dimensionless jet distance.Moreover,the axial velocity related to the nozzle with inclination angle 20°and 30°can produce a higher hole depth,while the radial velocity of the nozzle with 60°inclination can enlarge the hole diameter.The outcomes of the CFD simulations are consistent with the actual dynamic rock breaking and pore forming process,which lends credence to the present results and indicates that they could be used as a reference for the future optimization of systems based on the multi-hole combined external rotary bit technology.展开更多
基金Supported by Project of Development and Reform Commission of He'nan Province(2060403)~~
文摘[Objective] This study aimed to establish a tissue culture technology for Leucophyta brownii ‘Canal Rocks Form’. [Method] Leucophyta brownii ‘Canal Rocks Form’ stems with axillary buds were selected as explants in vitro to investigate the effects of different concentrations of 6-BA and NAA on proliferation culture and the effects of IBA, NAA and subculture cycle on rooting culture. [Result] The results showed that Leucophyta brownii ‘Canal Rocks Form’ plantlets need low concentrations of phytohormones and the rooting culture was significantly affected by the subculture cycle; the optimal medium for proliferation culture was MS＋0.5 mg/L 6-BA＋0.05 mg/L NAA＋30 g/L sucrose＋5.5 g/L agar, with a proliferation coefficient of 4.25; the plantlets with a subculture cycle of 28 d were the most suitable for rooting culture, with a rooting rate of 95.9% in 1/2 MS＋0.1 mg/L IBA＋0.05 mg/L NAA＋20 g/L sucrose＋5.5 g/L agar, and the average number and length of roots were 4.69 and 1.68 cm, respectively. [Conclusion] This study laid the foundation for establishing sterile culture system of Leucophyta brownii ‘Canal Rocks Form’.
基金the Science and Technology Innovation and Entrepreneurship Fund of China Coal Technology Engineering Group(2019-TD-QN038,2019-TDQN017)Enterprise Independent Innovation Guidance Project(2018ZDXM05,2019YBXM30).
文摘The characteristics of the flow field associated with a multi-hole combined external rotary bit have been studied by means of numerical simulation in the framework of an RNG k-εturbulence model,and compared with the results of dedicated rock breaking drilling experiments.The numerical results show that the nozzle velocity and dynamic pressure of the nozzle decrease with an increase in the jet distance,and the axial velocity of the nozzle decays regularly with an increase in the dimensionless jet distance.Moreover,the axial velocity related to the nozzle with inclination angle 20°and 30°can produce a higher hole depth,while the radial velocity of the nozzle with 60°inclination can enlarge the hole diameter.The outcomes of the CFD simulations are consistent with the actual dynamic rock breaking and pore forming process,which lends credence to the present results and indicates that they could be used as a reference for the future optimization of systems based on the multi-hole combined external rotary bit technology.
