Changes in Activities of the Key Enzymes Related to Starch Synthesis in Rice Grains During Grain Filling and Their Relationships with the Filling Rate and Cooking Quality

With 10 rice cultivars (lines) as materials, the changes in activities of adenosine diphosphoglucose pyrophosphorylase(ADPGPase), starch synthase (SSase) and starch branching enzyme (Q-enzyme) in the grains during grain filling, and theirrelationships with the filling rate, gel consistency (GC), alkali spreading value (ASV) and amylose content (AC) werestudied. The results showed that changes in activities of ADPGPase, SSase and Q-enzyme exhibited a single peak duringgrain filling, and the time of the activity peaks for the former two enzymes was earlier than that of the maximum grain-fillingrate (Tmax), and the time reaching the peak for Q-enzyme was synchronous with Tmax. The activities at early grain fillingstage, and the mean and maximum activities of each enzyme during grain filling period were positively and significantly orvery significantly correlated with the mean and maximum grain filling rate and starch content (mg grain-1) in the grains.Activities of ADPGPase at all grain filling stages and those of Q-enzyme at the early and mid filling stages were notsignificantly correlated the cooking quality (GC, ASV and AC). SSase activities at the early filling stage were significantlyand negatively correlated with GC and ASV, and positively correlated with AC. Activities of SSase at mid and late grainfilling stages and Q-enzyme at the late filling stage were significantly and positively correlated with GC and ASV, andnegatively correlated with AC. Spraying zeatin or abscisic acid at early grain filling stage could obviously regulate theactivities of ADPGPase, SSase and Q-enzyme in the grains.

Effects of Nitrogen Fertilizer Treatments on Caryopsis Filling and Respiratory Rate of Rice

An experiment was conducted to study the effects of nitrogen （N） rate and application time on grain filling and respiratory trait of caryopsis in two rice varieties, IR36 and Dali. The treatments were consisted of no N application topdressing at both tillering and booting stages （CK）, 6 g/pot of N topdressing at the tillering stage and 2 g/pot of N topdressing at the booting stage, 2 g/pot of N topdressing at the tillering stage and 6 g/pot of N topdressing at the booting stage. The results showed that the proper utilization of N fertilizer can be helpful to maintain the higher water content, higher respiratory rate and higher dehydrogenase activity of rice caryopsis in late filling phase, and prolong the course for filling and maintaining higher respiratory rate and dehydrogenase activity of rice caryopsis. More N application at booting was more effective compared to more N application at tillering.

Effects of Nitrogen Quantity on Grain-filling Characters of Two-line Hybrid Rice with Large Ear

[Objective] The aim was to research effects of N quantity on grain-filling characters of two-line hybrid rice cultivars with large ears. [Method] Peiza 67 and 88, two-line hybridized rice with large ears, were made use of to study on effects of N fertilizer in different quantities （LN： 90 kg/hm2;MN： 180 kg/hm2;HN： 270 kg/hm2） on plumpness and grain-filling characters. [Result] When N fertilizers were excessive, for inferior grains, grain-filling rate decreased and grain-filling time extended, resulting in plumpness decline after degradation of leaves＇ function. When N fertilizers were inadequate, maximal and average grain-filling rates decreased and the differences between superior and inferior grains in grain-filling rate increased, leading to decline of grain＇s weight and plumpness degree. On the other hand, quantity of N fertilizers had little effect on superior grains in plumpness. [Conclusion] The research provided references for reasonable use of N fertilizer and improvement of rice yield and N use.

Characteristic of laser-MIG hybrid welding with filling additional cold wire for aluminum alloy

The weld appearance, deposition rate, welding efficiency, stability of arc, laser keyhole characteristic, and weld property were studied by using a novel laser-MIG hybrid welding process with filling wire of aluminum alloy. The results were also compared with those by conventional laser-MIG hybrid welding process. It was found that with the suitable process parameters this novel welding process for aluminum alloy was stable and final weld bead had fine appearance. Compared to conventional laser-MIG hybrid welding process, during this novel welding process the stability of arc, the laser keyhole characteristic and the weld property were similar, while the keyhole cycle frequency and keyhole opening area had differences of 1.23% and 15.34%, respectively, and the welding efficiency increased by about 31% without increasing heat input.

Grain-filling characteristics and yield differences of maize cultivars with contrasting nitrogen efficiencies

To investigate the effect of nitrogen management on the grain-filling characteristics and yield formation of maize cultivars with contrasting nitrogen efficiencies,and to identify differences in grain-filling characteristics and yield of maize cultivars in response to nitrogen management,a two-year field experiment was conducted in southwest China in2015–2016.The grain-filling rate and duration of the N-inefficient cultivar XY 508 were higher than those of the N-efficient cultivar ZH 311.The 100-kernel weight of XY 508 was significantly higher than that of ZH 311.The kernel number per ear of ZH 311 was significantly higher than that of XY 508,making the population filling rate of ZH 311 significantly higher than that of XY 508.The higher population filling rate of the N-efficient maize cultivar led to a significant yield advantage over the N-inefficient maize cultivar.Nitrogen management effectively improved maize grain yield,but the response of maize cultivars with contrasting nitrogen efficiencies to nitrogen management was inconsistent.A basal fertilizer ratio 60.43%with a topdressing ratio 39.57%effectively increased grainfilling rate,delayed the time to maximum filling rate,prolonged the active filling period and effective grain-filling time,increased the 100-kernel weight,and maintained higher kernels per ear,thereby improving the population filling rate and maximizing the yield advantage of the N-efficient cultivar.A 100%basal fertilizer ratio not only increased the number of kernels per ear,but also maintained high grain filling characteristics to obtain a higher 100-kernel weight and increased the population filling rate,leading to a high grain yield in the N-inefficient cultivar.Thus,the 100%basal fertilizer ratio partially compensated for the deficient grain yield of the N-inefficient cultivar.

Microscopic Pore and Filling Performance of Coal Gangue Cementitious Paste

To obtain the influence laws of the fine gangue rate on the properties of coal gangue cementitious paste, the slump, divergence, stratification, bleeding, setting time and mechanical strength with the change of fine gangue rate were studied on the basis of keeping the amount of cementing material and slurry concentration unchanged. The porosity and the distribution of pore diameter of the filling specimen for curing 28 d were tested by a mercury injection instrument under different fine gangue rate conditions. It was shown that the slump, divergence, setting time and compressive strength of the paste firstly increased and then decreased with increasing fine gangue rate. The stratification and bleeding rate decreased with increasing fine gangue rate. The smaller the critical pore size of the paste was, the smaller the porosity was, the smaller the average pore size was. When the fine gangue rate was 40%, the maximum critical pore diameter of the paste was 55.79 μm, and the corresponding porosity was 17.54%, and the properties of filling paste were the best. When the fine gangue rate further increased, the aggregate surface area increased, and the reaction product of cementitious materials could not effectively fill the pores. It weakened the agglomeration effect. The particles surface of coal gangue was fragmental and flake deposit with irregular shape and uneven fold morphology. It was easy to be bonded with the surface of other filling material. The hydration products of coal gangue cementitious material were a large number of C-S-H gel with fibrous shape and ettringite（AFt） with compact block structure. The theoretical reference was provided for the preparation of low cost gangue cemented filling materials in coal mines.

The Endosperm-Specific Expression of YUCCA Genes Enhances Rice Grain Filling

Grain filling is a crucial process that affects yield in rice(Oryza sativa L.).Auxin biosynthesis and signaling are closely related to rice yield;therefore,it is important to understand the effects of auxin biosynthesis on rice grain filling to improve crop yield.In this study,we used physiological and molecular strategies to identify the roles of auxin in rice grain filling.Exogenous application of auxin(IAA)or auxin analogues(2,4-D)to young spikelets and flag leaves improved the seed-setting rate and yield per spike.Furthermore,real-time quantitative PCR assays confirmed that nine members of the OsYUCCA family of auxin biosynthetic genes were upregulated during grain filling,implication that auxin biosynthesis plays a major role in grain development.The specific expression of either Arabidopsis AtYUCCA1 or OsYUCCA2 in the endosperm or leaves resulted in increased expression of OsIAA genes and auxin content of seeds,as well as increased grain filling and seed-setting rate.This result establishes that the auxin content in grains and leaves is important for grain development.Our findings further highlight the potential applications for improving rice yield by elevating targeted gene expression in specific tissues.

WORST SUBCARRIER AVOIDING WATER-FILLING SUBCARRIER ALLOCATION SCHEME FOR OFDM-BASED CRN

Efficient and reliable subcarrier power joint allocation is served as a promising problem in cognitive OFDM-based Cognitive Radio Networks (CRN). This paper focuses on optimal subcarrier allocation for OFDM-based CRN. We mainly propose subcarrier allocation scheme denoted as Worst Subcarrier Avoiding Water-filling (WSAW), which is based on Rate Adaptive (RA) criterion and three constraints are considered in CRN. The algorithm divides the assignment procedure into two phases. The first phase is an initial subcarrier allocation based on the idea of avoiding selecting the worst subcarrier in order to maximize the transmission rate; while the second phase is an iterative adjustment process which is realized by swapping pairs of subcarriers between arbitrary users. The proposed scheme could assign subcarriers in accordance with channel coherence time. Hence, real time subcarrier allocation could be implemented. Simulation results show that, comparing with the similar existing algorithms, the proposed scheme could achieve larger capacity and a near-optimal BER performance.

Investigation of Upstream Near-Far Problem in VDSL Systems via Complete Adaptive Iterative Water Filling Algorithm

Crosstalk is the main degrading factor in Digital Subscriber Line (DSL) systems which are the result of electromagnetic coupling between two adjacent twisted pairs in a cable. Very-high bit-rate Digital Subscriber Line (VDSL) systems which use higher frequencies for data transmission than the other DSL systems, this effect is more considerable in Bit Error Rate (BER) degradation. This paper considers a complete adaptive iterative water-filling (IWF) algorithm for Resolving Upstream Near-Far Problem in VDSL Systems. The new distributed dynamic spectrum management algorithm is proposed, which improve achievable bit rate of iterative water-filling algorithm. The paper proffers a new power back-off strategy of the spectral mask at the near-end users, in order to protect the far-end users. Simulation of the proposed algorithm indicates that the bit rate is increased considerably rather the IWF and adaptive water-filling (AIWF) algorithms by keeping their low complexity. Furthermore, by adding the number of users in network, the new algorithm achieves performance gains over the AIWF, completely adaptive.

灌浆期高温对早稻光合性能和产量品质的影响

为研究不同高温条件对灌浆期早稻生长的影响及高温热害发生机理和灾害指标,以主推品种‘湘早籼45号’为材料,利用人工气候室开展早稻不同高温强度(35、37、38、39、40和42℃)和持续天数(3、5、7和10d)处理的试验,以同期田间自然条件为对照,分析不同高温条件对早稻光合性能和产量品质的影响,研究早稻高温热害致灾机理和等级指标。结果表明:与对照相比,高温处理降低了剑叶叶绿素含量、净光合速率、蒸腾速率和气孔导度,增加了秕谷率,降低了千粒重和产量,变幅与温度强度和持续天数成正比。高温降低了精米率、整精米率和直链淀粉含量,增加了垩白粒率、垩白度和蛋白质含量,改变了淀粉RVA谱特征。早稻高温热害等级指标为:35≤日最高气温(T_(max))<37℃且持续天数(D_(n))≥3 d或37≤T_(max)<38℃且3≤D_(n)<6 d属轻度,对应0<有害积热(H_(a))≤11℃·d,0<产量降幅(Y_(d))≤10%;37≤T_(max)<38℃且D_(n)≥6 d或T_(max)≥38℃且3≤D_(n)<6 d属中度,11<H_(a)≤22℃·d,10%<Y_(d)≤20%;T_(max)≥38℃且D_(n)≥6d为重度,H_(a)>22℃·d,Y_(d)>20%。因此,日最高气温≥35℃持续3d会降低早稻光合性能,影响产量品质形成,引起高温热害;根据灾害指标可预估高温热害程度。研究结果可为早稻高温热害监测评估和农业防灾减灾提供科学参考。

热牙胶垂直加压根管充填与冷牙胶侧方加压根管充填治疗慢性牙髓炎患者的效果比较

目的:比较热牙胶垂直加压根管充填与冷牙胶侧方加压根管充填治疗慢性牙髓炎患者的效果。方法:选取2021年3月至2023年3月该院收治的100例慢性牙髓炎患者进行前瞻性研究,按照随机数字表法将其分为研究组与对照组各50例。对照组采用冷牙胶侧方加压根管充填治疗,研究组采用热牙胶垂直加压根管充填治疗,比较两组恰填率、血清超敏C反应蛋白(hs-CRP)水平、上颌第一磨牙咬合力和并发症发生率。结果:研究组恰填率为90.00%,高于对照组的74.00%,差异有统计学意义(P<0.05);治疗后2周,两组上颌第一磨牙咬合力高于治疗前,且研究组高于对照组,两组血清hs-CRP水平低于治疗前,且研究组低于对照组,差异均有统计学意义(P<0.05);两组并发症发生率比较,差异无统计学意义(P>0.05)。结论:热牙胶垂直加压根管充填治疗慢性牙髓炎患者可提高恰填率和上颌第一磨牙咬合力,以及降低血清hs-CRP水平,效果优于冷牙胶侧方加压根管充填治疗。

基于进排气装置填充泡沫应用性能研究

分析了进排气装置的结构组成和填充泡沫成型工艺,运用ANSYS Workbench中的热力学模块对进排气装置填充泡沫隔热性能进行了仿真计算,并开展了填充泡沫样件隔热和吸水率试验。仿真和实测结果表明:填充泡沫隔热性能显著,能够减少排气管内180℃高温废气所含热量传导至进气管表面,使进气管表面的温度降至61℃,且在0.15 MPa压力保压24 h后最大吸水率为0.72%,满足隔热和吸水率使用要求,释放了风险,为其应用提供了坚实的理论支持。

土壤渍水胁迫对华北冬小麦籽粒灌浆及干物质分配的影响

利用少雨旱区华北冬麦主产区河北固城站的电动防雨棚,遮去自然降水,通过人工控制灌水形成土壤渍水、高湿、干旱和对照,冬小麦花后通过测定籽粒灌浆进程和地上生物量以及产量构成要素,解析不受阴雨和低温胁迫影响下土壤渍水对冬小麦籽粒灌浆速率及产量形成的胁迫效应。结果表明,随土壤水分的减少,冬小麦灌浆速率降低,灌浆持续日数缩短;灌浆期土壤高湿有增产效应,理论产量增产5.87%,土壤渍水出现减产,理论产量减产1.50%;高湿和渍水的收获指数比对照略有提高,并均高于0.5000;干旱胁迫下,收获指数比对照低0.1130~0.1633。北方旱区灌溉解除了气候干旱对作物需水胁迫,晴好天气光照充足,日较差大,作物产量提高,土壤水分是影响北方冬小麦挖掘光温生产潜力和提高单产的关键限制因素。研究结果为应对气候变化引起极端降水事件对农业生产的影响,尤其是科学评估旱、涝灾害对北方旱区农作物的影响有一定参考意义。

车用液氢气瓶快充过程数值研究

车用液氢气瓶加注过程中,因加注速度快且气瓶容积小,极易造成过充现象,导致安全事故发生。针对设有气包防过充装置的车载液氢气瓶建立三维对称模型,采用CFD方法对气瓶快速加注过程开展数值仿真。分析了开孔孔径、加注速率、初始充满率和防过充装置容积对防过充装置性能的影响规律。结果表明:开孔孔径是影响防过充装置性能的主要因素,其限制了液体的流通能力;采用6 mm孔径和初始充装量为50%的状况下,充装结束后气瓶都将过充,最大充装率可达98.4%;采用大容积的防过充装置可以为气瓶预留更多的气相安全空间,但同时也会导致充装量不足等问题,当防过充装置容积为100 L时,最终充装量仅有77.2%。

自体脂肪颗粒联合PRF移植填充矫治面部凹陷的效果及PRF对移植脂肪成活率的影响

目的:研究自体脂肪颗粒联合富血小板纤维蛋白(Platelet-rich fibrin,PRF)移植填充矫治面部凹陷的效果及PRF对移植脂肪成活率的影响。方法:选取2019年3月-2021年12月在笔者医院择期行脂肪颗粒移植填充手术的120例面部凹陷女性就医者,按照随机分组原则分为观察组和对照组,每组60例。两组均采取脂肪颗粒移植手术,观察组在移植自体脂肪颗手术基础上联合富血小板纤维蛋白治疗。术后6个月,比较两组移植脂肪的成活率及治疗效果。结果:观察组移植成活率显著高于对照组(P<0.05),且观察组治疗效果显著优于对照组(P<0.05);两组血肿情况、局部感染情况、坏死、脂肪液化、破溃、纤维囊肿发生情况比较,差异无统计学意义(P>0.05)。结论:自体脂肪颗粒移植联合PRF在面部凹陷填充中疗效确切,不仅提升了脂肪移植成活率,而且改善了面部凹陷情况,值得临床推广应用。

粉煤灰浆液氨吹脱及其膏体制备特性

为改善含氨粉煤灰对充填膏体性能带来的不利影响,提高粉煤灰的利用率,实验采用空气吹脱粉煤灰浆液的方式进行脱氨,探究吹脱时间、气浆比、固液比、温度和加碱量等影响因素对粉煤灰脱氨率的影响.结果表明,通过响应面优化吹脱条件,控制吹脱时间1h、气浆比900、固液比0.219、温度26.785℃和加碱量1.802%,预测总脱氨率最高达97.178%,与实际误差率在2%以内.经优化吹脱后所制备的粉煤灰充填膏体,仅在养护前期有少量的氨气释放,其泵送性能和微观结构相较于原含氨粉煤灰膏体都得到改善,且在养护28d后,抗压强度提升高达57%.

旱、渍胁迫对夏玉米根冠比及籽粒灌浆源库关系的影响

利用河北固城站电动防雨棚,遮去自然降水,人工控制灌水形成土壤渍水、高湿、干旱和对照,夏玉米开花后通过测定籽粒灌浆进程和地上生物量以及产量构成要素、根冠结构和根冠比等,探究不受阴雨和低温胁迫影响下的土壤旱、渍胁迫对夏玉米籽粒灌浆及产量形成的影响。分析夏玉米籽粒灌浆干物质的源库关系,结果表明:夏玉米籽粒灌浆对旱、渍胁迫的响应不同,渍水时灌浆持续日数延长,较对照延长7~8 d,重旱时缩短,较对照缩短5~6 d;对于平均灌浆速率,高湿最大,较对照偏大9.19%~9.85%,重旱最小,较对照偏小24.80%~25.26%,中度旱次小,较对照偏小6.89%~7.45%。旱、渍胁迫对夏玉米产量形成都有负效应:渍水、高湿减产率为1.83%~8.43%,干旱处理减产率为32.73%~81.96%,重旱近乎绝收,干旱危害程度比渍水、高湿影响大得多。旱、渍胁迫对夏玉米灌浆期穗位叶光合生理特性的影响不同,净光合速率(Pn)渍水比对照偏高0.91%~9.10%,高湿比对照偏低1.75%~9.13%;干旱比对照显著偏低,中度旱偏低55.76%~59.08%,重旱偏低92.16%~92.75%。夏玉米籽粒灌浆的干物质主要来源于灌浆期绿叶等光合作用形成的光合产物,成熟期地上干物质籽粒分配率减少,收获期秸秆存量大,导致收获指数降低。干旱胁迫刺激、诱导玉米根系增生须根,须根重增加显著,导致其根冠比提高,尤其重旱较渍水、高湿和对照提高近10倍以上,揭示出玉米根系尤其须根系对土壤水分逆境胁迫的应急响应生理调节机制和适应逆境条件的生存机理。实际生产管理中可利用土壤干湿交替变化刺激、诱发根系生长发育,提升优化根冠结构。研究结果为评估旱、涝灾害对北方旱区农作物生长发育和产量形成的影响提供数据支撑和参考。

施氮量对不同夏玉米品种籽粒灌浆特性、产量和品质的影响

以脱水速率不同的2个夏玉米品种迪卡517(DK 517,脱水速率较快)和郑单1002(ZD 1002,脱水速率较慢)为材料,研究施氮量[0 kg/hm^(2)(N0)、180 kg/hm^(2)(N1)、300 kg/hm^(2)(N2)]对夏玉米籽粒灌浆特性、脱水速率、产量和品质的影响,以期为黄淮海夏玉米优质高效生产提供理论依据。结果表明,随着施氮量的增加,DK 517和ZD 1002籽粒干质量总体上均逐渐增加,行粒数和产量先增加后降低,籽粒粗蛋白含量增加,粗淀粉含量降低。N1处理总体上籽粒灌浆速率最大时的生长量和最大灌浆速率最高,活跃灌浆期最长,行粒数最多,进而产量最高,分别为9694.60 kg/hm^(2)和11204.56 kg/hm^(2),且籽粒粗蛋白含量较高。不同施氮量处理对不同脱水类型夏玉米品种籽粒灌浆特性影响不同。授粉后15~24 d,不同处理夏玉米籽粒含水量之间差异显著,授粉后58 d,DK 517籽粒含水量表现为N2>N1>N0,ZD 1002籽粒含水量表现为N1>N0>N2。授粉后49~58 d,N0处理籽粒脱水速率最高。与ZD 1002相比,DK 517籽粒灌浆速率最大时的生长量和最大灌浆速率较高,到达最大灌浆速率的时间较早,活跃灌浆期较短,籽粒含水量较低,灌浆前期籽粒脱水速率较低,中后期较高,行粒数、百粒质量和产量较低,籽粒粗脂肪含量较低,粗蛋白和粗淀粉含量较高。籽粒脱水速率与穗粗、秃尖长呈显著或极显著负相关,与穗长和粗淀粉含量呈显著或极显著正相关。综上,施氮180 kg/hm^(2)可促进夏玉米籽粒灌浆,提高产量和籽粒粗蛋白含量。

北京市2011-2022年5岁儿童乳牙龋纵向变化分析

目的了解北京市5岁儿童口腔健康状况及趋势,对自2011年开始每年开展的3~5岁儿童氟化泡沫项目进行效果评价。方法对照三次流调抽样原则,进行分层、整群随机抽样,每个区县根据经济水平高低随机各抽取3所幼儿园5岁儿童进行调查,每所幼儿园抽取60人。分析其患龋率、龋均及充填率等变化情况。结果2011年患龋率和龋均为最高值,2012-2022年患龋率及龋均均低于2011年,并自2018年后逐渐趋于稳定。充填率呈上升趋势;SiC指数位于7~8之间。患龋率、龋均及充填率男女对比无差异(P>0.05),城乡有差异(P<0.05)。结论从2011年起在全北京市范围内开展氟化泡沫等综合龋病预防项目以来,北京市患龋情况得以初步控制,但仍需进一步加强防治工作。