The addition of ectomycorrhizal fungi(ECMF),beneficial rhizosphere microorganisms,to the soil can pro-mote plant growth and resistance.Here,Populus davidi-ana×Populus bolleana tissue culture seedlings were grown ...The addition of ectomycorrhizal fungi(ECMF),beneficial rhizosphere microorganisms,to the soil can pro-mote plant growth and resistance.Here,Populus davidi-ana×Populus bolleana tissue culture seedlings were grown for 3 months in soils inoculated with one of the species,then seedlings were assessed for mycorrhizal colonization rate and growth,physiological and root traits.Suillus luteus and Populus involutus each formed ectomycorrhizal associa-tions with the seedlings.Seedling height,ground diameter,biomass,and leaf area were significantly greater after treat-ment with ECMF than in the non-inoculated controls.Treat-ment improved all physiological and root variables assessed(chlorophylls and carotenoids,cellulose,and soluble sugars and proteins;root length,surface area,projected area,mean diameter,volume,number of root tips).Seedlings inocu-lated with S.luteus outperformed those inoculated with P.involutus.展开更多
Research efforts on electromagnetic interference(EMI)shielding materials have begun to converge on green and sustainable biomass materials.These materials offer numerous advantages such as being lightweight,porous,and...Research efforts on electromagnetic interference(EMI)shielding materials have begun to converge on green and sustainable biomass materials.These materials offer numerous advantages such as being lightweight,porous,and hierarchical.Due to their porous nature,interfacial compatibility,and electrical conductivity,biomass materials hold significant potential as EMI shielding materials.Despite concerted efforts on the EMI shielding of biomass materials have been reported,this research area is still relatively new compared to traditional EMI shielding materials.In particular,a more comprehensive study and summary of the factors influencing biomass EMI shielding materials including the pore structure adjustment,preparation process,and micro-control would be valuable.The preparation methods and characteristics of wood,bamboo,cellulose and lignin in EMI shielding field are critically discussed in this paper,and similar biomass EMI materials are summarized and analyzed.The composite methods and fillers of various biomass materials were reviewed.this paper also highlights the mechanism of EMI shielding as well as existing prospects and challenges for development trends in this field.展开更多
BACKGROUND Complicated crown–root fracture (CRF) involves severe injury to the crown, root,and pulp, and may be accompanied by multiple root fractures. The loss of a toothhas lifelong consequences for children and te...BACKGROUND Complicated crown–root fracture (CRF) involves severe injury to the crown, root,and pulp, and may be accompanied by multiple root fractures. The loss of a toothhas lifelong consequences for children and teenagers, but the maintenance of pulphealth and the calcific healing of multiple root fractures are rarely reported in theliterature.CASE SUMMARY This case reports healing of a permanent tooth with complicated crown–root andadditional root fractures, in which pulp health was maintained. A 10-year-old girlfell and fractured the root of her maxillary left central incisor at the cervical level.After the coronal fragment was repositioned, the tooth was splinted until thetooth was no longer mobile, 2 years later. Eight years after treatment, the toothhas remained asymptomatic with vital pulp and localized gingival overgrowth.Cone-beam computed tomography revealed not only calcified healing of the CRFbut also spontaneous healing in an additional undiagnosed root fracture. Thefracture line on the enamel could not be healed by hard tissue and formed agroove in the cervical crown. It was speculated that the groove was related to thelocalized gingival overgrowth.CONCLUSION This case provides a clinical perspective of the treatment of a tooth with acomplicated CRF and an additional root fracture.展开更多
China's forests cover 208.3 million ha and span a wide range of climates and a large variety of forest types, including tropical, temperate, and boreal forests. However, the variation patterns of fine root (< 2...China's forests cover 208.3 million ha and span a wide range of climates and a large variety of forest types, including tropical, temperate, and boreal forests. However, the variation patterns of fine root (< 2 mm in diameter) biomass, production, and turnover from the south to the north are unclear. This study summarizes fine root biomass (FRB), production (FRP) and turnover rate (FRT) in China's forests as reported by 140 case studies published from 1983 to 2014. The results showed that the mean values of FRB, FRP and FRT in China's forests were 278 g m(-2), 366 g m(-2) a(-1), and 1.19 a(-1), respectively. Compared with other studies at the regional or global scales, FRB in China's forests was lower, FRP was similar to estimates at the global scale, but FRT was much higher. FRB, FRP, and FRT in China's forests increased with increasing mean annual precipitation (MAP), indicating that fine root variables were likely related to MAP, rather than mean annual temperature or latitude. This is possibly due to the small variation in temperature but greater variation in precipitation during the growing season. These findings suggest that spatiotemporal variation in precipitation has a more profound impact on fine root dynamics in China's forests, and this will impact carbon and nutrient cycles driven by root turnover in the future.展开更多
Environmental heterogeneity is a constant presence in the natural world that significantly affects plant behavior at a variety of levels of complexity. In order to estimate the spatial pattern of fine root biomass in ...Environmental heterogeneity is a constant presence in the natural world that significantly affects plant behavior at a variety of levels of complexity. In order to estimate the spatial pattern of fine root biomass in the Three Gorges Reservoir Area, the spatial heterogeneity of fine root biomass in the upper layer of soils (0-10 cm) in three Mas- son pine (Pinus massoniana) stands in the Three Gorges Reservoir Area, China, was studied in 30 m x 30 m plots with geostatistical analysis. The results indicate that 1) both the live and dead fine root biomass of stand 2 were less than those of other stands, 2) the spatial variation of fine roots in the three stands was caused together by structural and ran- dom factors with moderate spatial dependence and 3) the magnitude of spatial heterogeneity of live fine roots ranked as: stand 3 〉 stand 1 〉 stand 2, while that of dead fine roots was similar in the three stands. These findings suggested that the range of spatial autocorrelation for fine root biomass varied considerably in the Three Gorges Reservoir Area, while soil properties, such as soil bulk density, organic matter and total nitrogen, may exhibit great effect on the spatial distribution of fine roots. Finally, we express our hope to be able to carry out further research on the quantitative relation- ship between the spatial heterogeneous patterns of plant and soil properties.展开更多
Biomass is an important component of global carbon cycling and is vulnerable to climate change. Previous studies have mainly focused on the responses of aboveground biomass and phenology to warming, while studies of r...Biomass is an important component of global carbon cycling and is vulnerable to climate change. Previous studies have mainly focused on the responses of aboveground biomass and phenology to warming, while studies of root architecture and of root biomass allocation between coarse and fine roots have been scarcely reported in grassland ecosystems. We conducted an open-top-chamber warming experiment to investigate the effect of potential warming on root biomass and root allocation in alpine steppe on the north Tibetan Plateau. The results showed that Stipa purpurea had significantly higher total root length, root surface area and tips than Carex moocroftii. However,there were no differences in total root volume, mean diameter and forks for the two species. Warming significantly increased total root biomass(27.60%), root biomass at 0–10 cm depth(27.84%) and coarse root biomass(diameter > 0.20 mm, 57.68%) in the growing season(August). However, warming had no significant influence on root biomass in the non-growing season(April). Root biomass showed clear seasonalvariations: total root biomass, root biomass at 0–10 cm depth and coarse root biomass significantly increased in the growing season. The increase in total root biomass was due to the enhancement of root biomass at 0–10 cm depth, to which the increase of coarse root biomass made a great contribution. This research is of significance for understanding biomass allocation, carbon cycling and biological adaptability in alpine grassland ecosystems under future climate change.展开更多
【Title】【Author】【Addresses】1The tree root distribution pattern and biomass of seventeen year old trees of Grewia optiva, Morus alba, Celtis australis, Bauhinia variegata and Robinia pseudoacacia were studied by e...【Title】【Author】【Addresses】1The tree root distribution pattern and biomass of seventeen year old trees of Grewia optiva, Morus alba, Celtis australis, Bauhinia variegata and Robinia pseudoacacia were studied by excavation method. B. variegata roots penetrated to a maximum depth of 4.78 m, whereas, M. alba roots were found down to 1.48 m depth. Lateral spread was minimum in B. variegata (1.10 m)and maximum inR. pseudoacacia (7.33 m). Maximum root biomass of 6.30 kg was found in R. pseudoacacia and minimum (2.43 kg) was found in M. alba. For four species viz.,G. optiva, M. alba, C. australis andR. pseudoacacia, 68%-87% root biomass occurred within top 0-30 cm soil depth, but forB. variegata this was only45%. The soil binding factor was maximum in G. optiva and minimum in B. variegata. Soil physico-chemical properties also showed wide variation. The study suggests thatB. variegata with a deep root system is the most suitable species for plantation under agroforestry systems. R. pseudoacacia and G. optiva with deep root systems, more lateral spread and high soil binding factor are suitable for plantation on degraded lands for soil conservation.展开更多
[Objective] The aim was to explore biomass and growth of Cupressus lusitanica seedlings in order to provide technological supports for afforestation. [Method] The introduced Cupressus lusitanica seedlings at different...[Objective] The aim was to explore biomass and growth of Cupressus lusitanica seedlings in order to provide technological supports for afforestation. [Method] The introduced Cupressus lusitanica seedlings at different ages were re- searched to explore biomass (ground or underground) morphological shape, surface area and volume of a root system. [Result] As age grew, plant height and ground diameter increased significantly, varying dramatically upon seed provenance, and root-canopy ratio, root length, surface area and volume of seedlings were increas- ing. Specifically, seedlings at two-year-old and three-year-old kept a stable growth; moderate and big roots took higher proportions in a root system; underground parts for a plant required more photosynthetic products; root systems of young seedlings grew to moderate and big roots. [Conclusion] Ground biomass is of correlation with root system indices and the morphological characteristics of root systems are close to biomass. The accumulation capacity of biomass differed for seedlings from differ- ent provenances, even in the same environment and at the same time.展开更多
Serendipita indica(formerly known as Piriformospora indica),a root endophytic fungus,exhibits multiple functions in some agricultural,horticultural,and medicinal plant species.We studied colonization of the roots of P...Serendipita indica(formerly known as Piriformospora indica),a root endophytic fungus,exhibits multiple functions in some agricultural,horticultural,and medicinal plant species.We studied colonization of the roots of Platycladus orientalis,a forest tree species,by S.indica to improve the quality of the seedlings in seedbeds and survival rates in sylviculture.At 20 days after inoculation,S.indica colonized the root cortex of P.orientalis seedlings.Root colonization by S.indica significantly increased net CO2 assimilation,light use efficiency,and biomass accumulation by both roots and shoots,whereas it did not affect the biomass allocation between roots and shoots.In addition,the symbiosis significantly increased root total length,surface area,and volume.In view of the two specific traits of S.indica,i.e.,axenic culture and wide colonization in plants,the fungus might be used for improving quality of P.orientalis seedlings and increasing their survival after transplanting.展开更多
Objective:To increase biomass and saponin production in hairy root culture of Talinum paniculatum Gaertn.(T.paniculatum)in balloon-type bubble bioreactor(BTBB).Methods:Hairy roots which were collected from leaf explan...Objective:To increase biomass and saponin production in hairy root culture of Talinum paniculatum Gaertn.(T.paniculatum)in balloon-type bubble bioreactor(BTBB).Methods:Hairy roots which were collected from leaf explants of T.paniculatum were infected by Agrobacterium rhizogenes strain LB510.The hairy roots were cultivated at400 m L Murashige and Skoog liquid medium without growth regulator(MS0)in1 000 m L BTBB.Each BTBB had 2 g hairy roots as initial inoculum and these cultures were treated with various concentrations of sucrose(3%,4%,5%,6%w/v)and potassium nitrate(0.5,1.0,1.5 and 2.0 strength of MS medium).Cultures were maintained for 14days.Fresh and dry weights of hairy roots at the end of culture were investigated.Results:Various concentrations of sucrose influenced the biomass accumulation of hairy roots.Maximum biomass was reached by MS medium supplemented with 6%sucrose and it was approximately threefold higher than control.Culture supplemented with potassium nitrate at 2.0 strength of MS0 could increase biomass accumulation of hairy roots until 0.14 g dry weight and it was almost threefold higher than control.However,the maximum saponin content was obtained by MS medium supplemented with 5%sucrose and 2.0 strength potassium nitrate of MS.Conclusions:Based on this research,those conditions can be used to produce biomass and saponin of hairy root of T.paniculatum in the large scale.展开更多
Background:Thinning is a commonly used treatment in forest management which affects the tree root systems.The effects of thinning on element concentrations and seasonal change of roots were evaluated in adjacent oak(Q...Background:Thinning is a commonly used treatment in forest management which affects the tree root systems.The effects of thinning on element concentrations and seasonal change of roots were evaluated in adjacent oak(Quercus frainetto Ten.)and hornbeam(Carpinus betulus L.)stands according to the different root diameter classes.Method:Two replicated control and thinning plots(50m×50 m)were set for each species(hornbeam and oak).Thinning treatments(November 2010)reduced 50%of the basal area in both oak and hornbeam stands.Roots were assessed by seasonal collection over 2 years(from October 2010 to October 2012).The roots were then sorted into diameter classes of 0–2mm(fine roots),2–5mm(small roots)and>5mm(coarse roots).C,N,P,K,Ca,Na,Mg,S,Mn,Fe,Al,Zn,Pb,Ni,Cu and Cd were analyzed.Results:Except coarse roots,the highest root biomasses were determined in April-2011 in all plots.Fine-root biomass in oak was found significantly higher in control plots.In contrast to the oak,the fine-root biomass in the thinned hornbeam plots was higher than in the controls.The small-root biomass did not significantly differ between the thinned and the control plots in both oak and hornbeam stands.However,the coarse-root biomass showed significant differences between the control(1989 g∙m^(−2))and thinned plots(1060 g∙m^(−2))in oak,while no difference was detected in hornbeam.The concentrations of C,Al,Pb,Cd,Ni,Zn,Mn,Na,K,Mg and P in the fine roots of oak were significantly higher in the thinned plots.However,the concentration of Pb,Cd and Fe in the fine roots was significantly higher in the thinned plots of hornbeam.Significant differences were observed between the species for all elements in the fine roots except for C,N and P.In particular,elements in the fine roots tended to increase in July in the oak.In the hornbeam,all element concentrations in the fine roots(except C,N,and S)in the thinned plots showed a tendency to increase in April.The concentrations of Pb,Ni,Al,Fe,Cu,Ca,Na,K,Mg and P in the hornbeam control plots increased during the April 2011 period.Conclusion:The results indicated that thinning effects on temporal changes and concentrations of elements in the roots could be attributed to species-specific characteristics.展开更多
Sugarcane is used worldwide for sugar, ethanol and energy production. In Brazil, the shift from burned to unburned harvest systems resulted in increases in nitrogen fertilization rates, which can impact root architect...Sugarcane is used worldwide for sugar, ethanol and energy production. In Brazil, the shift from burned to unburned harvest systems resulted in increases in nitrogen fertilization rates, which can impact root architecture and biomass. The expectation is also an increase in sugarcane biomass. The study hypothesized that high N rates applied to sugarcane fields increases root growth and N stored in roots, promoting higher biomass and N accumulated in shoots. Two experiments were set up in Southeastern Brazil, on a Typic Kandiudox (TK) and Rhodic Eutrudox (RE). Four treatments were studied 1) N application in the plant-cane (0 and 120 kg·ha-1 N) and 2) N application in the ratoon (0 and 150 kg·ha-1 N). The shoot biomass and the root density (by the core method up to 0.6 m) were evaluated over the first ratoon crop cycle, and the N content in those compartments was also examined. There was no carry over effect on N applied at planting in root and shoot biomass in the ratoon crop cycle. At the RE site, the ratoon N fertilization increased root density in the superficial soil layer (0 - 0.2 m) and close to the plants (<0.3 m). The effect of N addition on root biomass, and biomass and N accumulated in shoot was limited in both sites. Increasing N rates in unburned sugarcane fields do not consistently increases root and shoot biomass under Brazilian field conditions.展开更多
In order to understand its response towards nickel stress, watercress (Nasturtium officinale R. Br.) was exposed to nickel (1-25 mg/L) for 1, 3, 5 and 7 days. The accumulation and translocation of nickel were dete...In order to understand its response towards nickel stress, watercress (Nasturtium officinale R. Br.) was exposed to nickel (1-25 mg/L) for 1, 3, 5 and 7 days. The accumulation and translocation of nickel were determined and the influence of nickel on biomass, protein content and enzymatic antioxidants was examined for both roots and leaves. It was determined that N. o fficinale could accumulate appreciable amounts of Ni in both roots and leaves. Nickel accumulated particularly in the roots of plants. Biomass increased at low nickel concentrations but certain measurable change was not found at high concentrations. Under stress conditions the antioxidant enzymes were up-regulated compared to control. An increase in protein content and enzyme activities was observed at moderate exposure conditions followed by a decline at both roots and leaves. The maximum enzyme activities were observed at different exposure conditions. Our results showed that N. officinale had the capacity to overcome nickel-induced stress especially at moderate nickel exposure. Therefore, N. officinale may be used as a phytoremediator in moderately polluted aquatic ecosystems.展开更多
Background:Inputs of above-and belowground litter into forest soils are changing at an unprecedented rate due to continuing human disturbances and climate change.Microorganisms drive the soil carbon(C)cycle,but the ro...Background:Inputs of above-and belowground litter into forest soils are changing at an unprecedented rate due to continuing human disturbances and climate change.Microorganisms drive the soil carbon(C)cycle,but the roles of above-and belowground litter in regulating the soil microbial community have not been evaluated at a global scale.Methods:Here,we conducted a meta-analysis based on 68 aboveground litter removal and root exclusion studies across forest ecosystems to quantify the roles of above-and belowground litter on soil microbial community and compare their relative importance.Results:Aboveground litter removal significantly declined soil microbial biomass by 4.9%but root exclusion inhibited it stronger,up to 11.7%.Moreover,the aboveground litter removal significantly raised fungi by 10.1%without altering bacteria,leading to a 46.7%increase in the fungi-to-bacteria(F/B)ratio.Differently,root exclusion significantly decreased the fungi by 26.2%but increased the bacteria by 5.7%,causing a 13.3%decrease in the F/B ratio.Specifically,root exclusion significantly inhibited arbuscular mycorrhizal fungi,ectomycorrhizal fungi,and actinomycetes by 22.9%,43.8%,and 7.9%,respectively.The negative effects of aboveground litter removal on microbial biomass increased with mean annual temperature and precipitation,whereas that of root exclusion on microbial biomass did not change with climatic factors but amplified with treatment duration.More importantly,greater effects of root exclusion on microbial biomass than aboveground litter removal were consistent across diverse forest biomes(expect boreal forests)and durations.Conclusions:These data provide a global evidence that root litter inputs exert a larger control on microbial biomass than aboveground litter inputs in forest ecosystems.Our study also highlights that changes in above-and belowground litter inputs could alter soil C stability differently by shifting the microbial community structure in the opposite direction.These findings are useful for predicting microbe-mediated C processes in response to changes in forest management or climate.展开更多
Plant nitrogen assimilation and use efficiency in the seedling's root system are beneficial for adult plants in field condition for yield enhancement.Identification of the genetic basis between root traits and N u...Plant nitrogen assimilation and use efficiency in the seedling's root system are beneficial for adult plants in field condition for yield enhancement.Identification of the genetic basis between root traits and N uptake plays a crucial role in wheat breeding.In the present study,198 doubled haploid lines from the cross of Yangmai 16/Zhongmai 895 were used to identify quantitative trait loci(QTLs)underpinning four seedling biomass traits and five root system architecture(RSA)related traits.The plants were grown under hydroponic conditions with control,low and high N treatments(Ca(NO_(3))_(2)·4H_(2)O at 0,0.05 and 2.0 mmol L^(-1),respectively).Significant variations among the treatments and genotypes,and positive correlations between seedling biomass and RSA traits(r=0.20 to 0.98)were observed.Inclusive composite interval mapping based on a high-density map from the Wheat 660 K single nucleotide polymorphisms(SNP)array identified 51 QTLs from the three N treatments.Twelve new QTLs detected on chromosomes 1 AL(1)in the control,1 DS(2)in high N treatment,4 BL(5)in low and high N treatments,and 7 DS(3)and 7 DL(1)in low N treatments,are first reported in influencing the root and biomass related traits for N uptake.The most stable QTLs(RRS.caas-4 DS)on chromosome 4 DS,which were related to ratio of root to shoot dry weight trait,was in close proximity of the Rht-D1 gene,and it showed high phenotypic effects,explaining 13.1%of the phenotypic variance.Twenty-eight QTLs were clustered in 12 genetic regions.SNP markers tightly linked to two important QTLs clusters C10 and C11 on chromosomes 6 BL and 7 BL were converted to kompetitive allele-specific PCR(KASP)assays that underpin important traits in root development,including root dry weight,root surface area and shoot dry weight.These QTLs,clusters and KASP assays can greatly improve the efficiency of selection for root traits in wheat breeding programmes.展开更多
Understanding the effects of degradation on belowground biomass(BGB)is essential for assessment of carbon budget of the alpine meadow ecosystem on the Tibetan Plateau,China.This ecosystem has been undergoing serious d...Understanding the effects of degradation on belowground biomass(BGB)is essential for assessment of carbon budget of the alpine meadow ecosystem on the Tibetan Plateau,China.This ecosystem has been undergoing serious degradation owing to climate change and anthropogenic activities.This study examined the response of the vertical distribution of plant BGB to degradation and explored the underlying mechanisms in an alpine meadow on the Tibetan Plateau.A field survey was conducted in an alpine meadow with seven sequential degrees of degradation in the Zoige Plateau on the Tibetan Plateau during the peak growing season of 2018.We measured aboveground biomass(AGB),BGB,soil water content(SWC),soil bulk density(SBD),soil compaction(SCOM),soil organic carbon(SOC),soil total nitrogen(STN),soil total phosphorus(STP),soil available nitrogen(SAN),and soil available phosphorus(STP)in the 0-30 cm soil layers.Our results show that degradation dramatically decreased the BGB in the 0-10 cm soil layer(BGB0-10)but slightly increased the subsoil BGB.The main reason may be that the physical-chemical properties of surface soil were more sensitive to degradation than those of subsoil,as indicated by the remarked positive associations of the trade-off value of BGB0-10 with SWC,SCOM,SOC,STN,SAN,and STP,as well as the negative correlation between the trade-off value of BGB0-10 and SBD in the soil layer of 0-10 cm.In addition,an increase in the proportion of forbs with increasing degradation degree directly affected the BGB vertical distribution.The findings suggest that the decrease in the trade-off value of BGB0-10 in response to degradation might be an adaptive strategy for the degradation-induced drought and infertile soil conditions.This study can provide theoretical support for assessing the effects of degradation on the carbon budget and sustainable development in the alpine meadow ecosystem on the Tibetan Plateau as well as other similar ecosystems in the world.展开更多
Descriptions of tree root morphology inform design of belowground biomass and carbon inventories and sampling for research. We studied root morphology of tanoak (Notholithocarpus densiflorus), an important component i...Descriptions of tree root morphology inform design of belowground biomass and carbon inventories and sampling for research. We studied root morphology of tanoak (Notholithocarpus densiflorus), an important component in mixed evergreen forests of California and Oregon, USA. Tanoak re-sprouts from belowground lignotubers after disturbances, and stores an unknown amount of carbon in coarse roots underground. We sought to ascribe explanatory nomenclature to roots’ morphological features and to identify models describing tanoak root morphology. Twelve tanoak root systems were excavated, dissected, and measured. Roots tapered according to their circumference and location. Larger roots closer to the lignotuber (located at the base of the tree stem) tapered more rapidly per unit of length. Tanoak roots forked frequently. Root cross-sectional area was preserved after forking events (i.e., the sum of cross-sectional areas for smaller roots on one side of the fork correlated with the adjoining large root). Occurrence and quantity of root branches (small roots branching laterally from larger roots) was dependent upon length of the source root segment. Our models of tanoak root morphology are designed to be organized together to estimate biomass of any segment or collection of lateral roots (e.g., roots lost/missed during excavation, or in lieu of destructive sampling), given root diameter at a known distance from the lignotuber. The taper model gives distal- and proximal-end diameters for calculation of volume for segments of root tapering between forks. Frequency of forking and branching can also be predicted. Summing the predicted mass of each lateral root segment, branch, and forked segment would produce an estimate of mass for a contiguous network of lateral roots.展开更多
基金part of the Liaoning Provincial Department of Education project LJKZ0684supported by the National Natural Science Foundation of China (31800542)
文摘The addition of ectomycorrhizal fungi(ECMF),beneficial rhizosphere microorganisms,to the soil can pro-mote plant growth and resistance.Here,Populus davidi-ana×Populus bolleana tissue culture seedlings were grown for 3 months in soils inoculated with one of the species,then seedlings were assessed for mycorrhizal colonization rate and growth,physiological and root traits.Suillus luteus and Populus involutus each formed ectomycorrhizal associa-tions with the seedlings.Seedling height,ground diameter,biomass,and leaf area were significantly greater after treat-ment with ECMF than in the non-inoculated controls.Treat-ment improved all physiological and root variables assessed(chlorophylls and carotenoids,cellulose,and soluble sugars and proteins;root length,surface area,projected area,mean diameter,volume,number of root tips).Seedlings inocu-lated with S.luteus outperformed those inoculated with P.involutus.
基金National Natural Science Foundation of China(32201491)Young Elite Scientists Sponsorship Program by CAST(2023QNRC001)The authors extend their appreciation to the Deanship of Scientific Research at Northern Border University,Arar,KSA for funding this research work through the project number“NBU-FPEJ-2024-1101-02”.
文摘Research efforts on electromagnetic interference(EMI)shielding materials have begun to converge on green and sustainable biomass materials.These materials offer numerous advantages such as being lightweight,porous,and hierarchical.Due to their porous nature,interfacial compatibility,and electrical conductivity,biomass materials hold significant potential as EMI shielding materials.Despite concerted efforts on the EMI shielding of biomass materials have been reported,this research area is still relatively new compared to traditional EMI shielding materials.In particular,a more comprehensive study and summary of the factors influencing biomass EMI shielding materials including the pore structure adjustment,preparation process,and micro-control would be valuable.The preparation methods and characteristics of wood,bamboo,cellulose and lignin in EMI shielding field are critically discussed in this paper,and similar biomass EMI materials are summarized and analyzed.The composite methods and fillers of various biomass materials were reviewed.this paper also highlights the mechanism of EMI shielding as well as existing prospects and challenges for development trends in this field.
基金Supported by 2021 Disciplinary Construction Project in School of Dentistry,Anhui Medical University,No.2021kqxkFY05.
文摘BACKGROUND Complicated crown–root fracture (CRF) involves severe injury to the crown, root,and pulp, and may be accompanied by multiple root fractures. The loss of a toothhas lifelong consequences for children and teenagers, but the maintenance of pulphealth and the calcific healing of multiple root fractures are rarely reported in theliterature.CASE SUMMARY This case reports healing of a permanent tooth with complicated crown–root andadditional root fractures, in which pulp health was maintained. A 10-year-old girlfell and fractured the root of her maxillary left central incisor at the cervical level.After the coronal fragment was repositioned, the tooth was splinted until thetooth was no longer mobile, 2 years later. Eight years after treatment, the toothhas remained asymptomatic with vital pulp and localized gingival overgrowth.Cone-beam computed tomography revealed not only calcified healing of the CRFbut also spontaneous healing in an additional undiagnosed root fracture. Thefracture line on the enamel could not be healed by hard tissue and formed agroove in the cervical crown. It was speculated that the groove was related to thelocalized gingival overgrowth.CONCLUSION This case provides a clinical perspective of the treatment of a tooth with acomplicated CRF and an additional root fracture.
基金supported by Grants from the National Key Research and Development Program of China(2016YFD06004040604)the Natural Science Foundation of Heilongjiang Province(No.C2016004)
文摘China's forests cover 208.3 million ha and span a wide range of climates and a large variety of forest types, including tropical, temperate, and boreal forests. However, the variation patterns of fine root (< 2 mm in diameter) biomass, production, and turnover from the south to the north are unclear. This study summarizes fine root biomass (FRB), production (FRP) and turnover rate (FRT) in China's forests as reported by 140 case studies published from 1983 to 2014. The results showed that the mean values of FRB, FRP and FRT in China's forests were 278 g m(-2), 366 g m(-2) a(-1), and 1.19 a(-1), respectively. Compared with other studies at the regional or global scales, FRB in China's forests was lower, FRP was similar to estimates at the global scale, but FRT was much higher. FRB, FRP, and FRT in China's forests increased with increasing mean annual precipitation (MAP), indicating that fine root variables were likely related to MAP, rather than mean annual temperature or latitude. This is possibly due to the small variation in temperature but greater variation in precipitation during the growing season. These findings suggest that spatiotemporal variation in precipitation has a more profound impact on fine root dynamics in China's forests, and this will impact carbon and nutrient cycles driven by root turnover in the future.
基金supported by the Special Fund of National Forestry Public Welfare of the State Forestry Administration (No.201104008)a Special Fund of the Research Institute of Forest Ecology, Environment and Protection of the Chinese Academy of Forestry, China (No. CAFRIFEEP201006)
文摘Environmental heterogeneity is a constant presence in the natural world that significantly affects plant behavior at a variety of levels of complexity. In order to estimate the spatial pattern of fine root biomass in the Three Gorges Reservoir Area, the spatial heterogeneity of fine root biomass in the upper layer of soils (0-10 cm) in three Mas- son pine (Pinus massoniana) stands in the Three Gorges Reservoir Area, China, was studied in 30 m x 30 m plots with geostatistical analysis. The results indicate that 1) both the live and dead fine root biomass of stand 2 were less than those of other stands, 2) the spatial variation of fine roots in the three stands was caused together by structural and ran- dom factors with moderate spatial dependence and 3) the magnitude of spatial heterogeneity of live fine roots ranked as: stand 3 〉 stand 1 〉 stand 2, while that of dead fine roots was similar in the three stands. These findings suggested that the range of spatial autocorrelation for fine root biomass varied considerably in the Three Gorges Reservoir Area, while soil properties, such as soil bulk density, organic matter and total nitrogen, may exhibit great effect on the spatial distribution of fine roots. Finally, we express our hope to be able to carry out further research on the quantitative relation- ship between the spatial heterogeneous patterns of plant and soil properties.
基金supported by the National Natural Science Foundation of China (41571205)the Strategic Pilot Science and Technology Projects of Chinese Academy of Sciences (Grant No. XDB03030505, XDA05050506)
文摘Biomass is an important component of global carbon cycling and is vulnerable to climate change. Previous studies have mainly focused on the responses of aboveground biomass and phenology to warming, while studies of root architecture and of root biomass allocation between coarse and fine roots have been scarcely reported in grassland ecosystems. We conducted an open-top-chamber warming experiment to investigate the effect of potential warming on root biomass and root allocation in alpine steppe on the north Tibetan Plateau. The results showed that Stipa purpurea had significantly higher total root length, root surface area and tips than Carex moocroftii. However,there were no differences in total root volume, mean diameter and forks for the two species. Warming significantly increased total root biomass(27.60%), root biomass at 0–10 cm depth(27.84%) and coarse root biomass(diameter > 0.20 mm, 57.68%) in the growing season(August). However, warming had no significant influence on root biomass in the non-growing season(April). Root biomass showed clear seasonalvariations: total root biomass, root biomass at 0–10 cm depth and coarse root biomass significantly increased in the growing season. The increase in total root biomass was due to the enhancement of root biomass at 0–10 cm depth, to which the increase of coarse root biomass made a great contribution. This research is of significance for understanding biomass allocation, carbon cycling and biological adaptability in alpine grassland ecosystems under future climate change.
基金Indian Council of Agriculture Research, New Delhi for supporting the study through All India Coordinated Research Project on Agroforestry (AICRP)
文摘【Title】【Author】【Addresses】1The tree root distribution pattern and biomass of seventeen year old trees of Grewia optiva, Morus alba, Celtis australis, Bauhinia variegata and Robinia pseudoacacia were studied by excavation method. B. variegata roots penetrated to a maximum depth of 4.78 m, whereas, M. alba roots were found down to 1.48 m depth. Lateral spread was minimum in B. variegata (1.10 m)and maximum inR. pseudoacacia (7.33 m). Maximum root biomass of 6.30 kg was found in R. pseudoacacia and minimum (2.43 kg) was found in M. alba. For four species viz.,G. optiva, M. alba, C. australis andR. pseudoacacia, 68%-87% root biomass occurred within top 0-30 cm soil depth, but forB. variegata this was only45%. The soil binding factor was maximum in G. optiva and minimum in B. variegata. Soil physico-chemical properties also showed wide variation. The study suggests thatB. variegata with a deep root system is the most suitable species for plantation under agroforestry systems. R. pseudoacacia and G. optiva with deep root systems, more lateral spread and high soil binding factor are suitable for plantation on degraded lands for soil conservation.
基金Supported by National Program "948"(2009-4-17)~~
文摘[Objective] The aim was to explore biomass and growth of Cupressus lusitanica seedlings in order to provide technological supports for afforestation. [Method] The introduced Cupressus lusitanica seedlings at different ages were re- searched to explore biomass (ground or underground) morphological shape, surface area and volume of a root system. [Result] As age grew, plant height and ground diameter increased significantly, varying dramatically upon seed provenance, and root-canopy ratio, root length, surface area and volume of seedlings were increas- ing. Specifically, seedlings at two-year-old and three-year-old kept a stable growth; moderate and big roots took higher proportions in a root system; underground parts for a plant required more photosynthetic products; root systems of young seedlings grew to moderate and big roots. [Conclusion] Ground biomass is of correlation with root system indices and the morphological characteristics of root systems are close to biomass. The accumulation capacity of biomass differed for seedlings from differ- ent provenances, even in the same environment and at the same time.
基金funded by National Nature Science Foundation of China(31471496)
文摘Serendipita indica(formerly known as Piriformospora indica),a root endophytic fungus,exhibits multiple functions in some agricultural,horticultural,and medicinal plant species.We studied colonization of the roots of Platycladus orientalis,a forest tree species,by S.indica to improve the quality of the seedlings in seedbeds and survival rates in sylviculture.At 20 days after inoculation,S.indica colonized the root cortex of P.orientalis seedlings.Root colonization by S.indica significantly increased net CO2 assimilation,light use efficiency,and biomass accumulation by both roots and shoots,whereas it did not affect the biomass allocation between roots and shoots.In addition,the symbiosis significantly increased root total length,surface area,and volume.In view of the two specific traits of S.indica,i.e.,axenic culture and wide colonization in plants,the fungus might be used for improving quality of P.orientalis seedlings and increasing their survival after transplanting.
基金Supported by Grant from Universitas Airlangga,Surabaya,Indonesia with Grant No.8714/UN3/KR/2013
文摘Objective:To increase biomass and saponin production in hairy root culture of Talinum paniculatum Gaertn.(T.paniculatum)in balloon-type bubble bioreactor(BTBB).Methods:Hairy roots which were collected from leaf explants of T.paniculatum were infected by Agrobacterium rhizogenes strain LB510.The hairy roots were cultivated at400 m L Murashige and Skoog liquid medium without growth regulator(MS0)in1 000 m L BTBB.Each BTBB had 2 g hairy roots as initial inoculum and these cultures were treated with various concentrations of sucrose(3%,4%,5%,6%w/v)and potassium nitrate(0.5,1.0,1.5 and 2.0 strength of MS medium).Cultures were maintained for 14days.Fresh and dry weights of hairy roots at the end of culture were investigated.Results:Various concentrations of sucrose influenced the biomass accumulation of hairy roots.Maximum biomass was reached by MS medium supplemented with 6%sucrose and it was approximately threefold higher than control.Culture supplemented with potassium nitrate at 2.0 strength of MS0 could increase biomass accumulation of hairy roots until 0.14 g dry weight and it was almost threefold higher than control.However,the maximum saponin content was obtained by MS medium supplemented with 5%sucrose and 2.0 strength potassium nitrate of MS.Conclusions:Based on this research,those conditions can be used to produce biomass and saponin of hairy root of T.paniculatum in the large scale.
基金This work was supported by Scientific Research Projects Coordination Unit of Istanbul University-Cerrahpasa(Project No.FYD-2016-20102).
文摘Background:Thinning is a commonly used treatment in forest management which affects the tree root systems.The effects of thinning on element concentrations and seasonal change of roots were evaluated in adjacent oak(Quercus frainetto Ten.)and hornbeam(Carpinus betulus L.)stands according to the different root diameter classes.Method:Two replicated control and thinning plots(50m×50 m)were set for each species(hornbeam and oak).Thinning treatments(November 2010)reduced 50%of the basal area in both oak and hornbeam stands.Roots were assessed by seasonal collection over 2 years(from October 2010 to October 2012).The roots were then sorted into diameter classes of 0–2mm(fine roots),2–5mm(small roots)and>5mm(coarse roots).C,N,P,K,Ca,Na,Mg,S,Mn,Fe,Al,Zn,Pb,Ni,Cu and Cd were analyzed.Results:Except coarse roots,the highest root biomasses were determined in April-2011 in all plots.Fine-root biomass in oak was found significantly higher in control plots.In contrast to the oak,the fine-root biomass in the thinned hornbeam plots was higher than in the controls.The small-root biomass did not significantly differ between the thinned and the control plots in both oak and hornbeam stands.However,the coarse-root biomass showed significant differences between the control(1989 g∙m^(−2))and thinned plots(1060 g∙m^(−2))in oak,while no difference was detected in hornbeam.The concentrations of C,Al,Pb,Cd,Ni,Zn,Mn,Na,K,Mg and P in the fine roots of oak were significantly higher in the thinned plots.However,the concentration of Pb,Cd and Fe in the fine roots was significantly higher in the thinned plots of hornbeam.Significant differences were observed between the species for all elements in the fine roots except for C,N and P.In particular,elements in the fine roots tended to increase in July in the oak.In the hornbeam,all element concentrations in the fine roots(except C,N,and S)in the thinned plots showed a tendency to increase in April.The concentrations of Pb,Ni,Al,Fe,Cu,Ca,Na,K,Mg and P in the hornbeam control plots increased during the April 2011 period.Conclusion:The results indicated that thinning effects on temporal changes and concentrations of elements in the roots could be attributed to species-specific characteristics.
文摘Sugarcane is used worldwide for sugar, ethanol and energy production. In Brazil, the shift from burned to unburned harvest systems resulted in increases in nitrogen fertilization rates, which can impact root architecture and biomass. The expectation is also an increase in sugarcane biomass. The study hypothesized that high N rates applied to sugarcane fields increases root growth and N stored in roots, promoting higher biomass and N accumulated in shoots. Two experiments were set up in Southeastern Brazil, on a Typic Kandiudox (TK) and Rhodic Eutrudox (RE). Four treatments were studied 1) N application in the plant-cane (0 and 120 kg·ha-1 N) and 2) N application in the ratoon (0 and 150 kg·ha-1 N). The shoot biomass and the root density (by the core method up to 0.6 m) were evaluated over the first ratoon crop cycle, and the N content in those compartments was also examined. There was no carry over effect on N applied at planting in root and shoot biomass in the ratoon crop cycle. At the RE site, the ratoon N fertilization increased root density in the superficial soil layer (0 - 0.2 m) and close to the plants (<0.3 m). The effect of N addition on root biomass, and biomass and N accumulated in shoot was limited in both sites. Increasing N rates in unburned sugarcane fields do not consistently increases root and shoot biomass under Brazilian field conditions.
基金supported by Erciyes University Scientific Research Project Fund (No.FBA 07-32)
文摘In order to understand its response towards nickel stress, watercress (Nasturtium officinale R. Br.) was exposed to nickel (1-25 mg/L) for 1, 3, 5 and 7 days. The accumulation and translocation of nickel were determined and the influence of nickel on biomass, protein content and enzymatic antioxidants was examined for both roots and leaves. It was determined that N. o fficinale could accumulate appreciable amounts of Ni in both roots and leaves. Nickel accumulated particularly in the roots of plants. Biomass increased at low nickel concentrations but certain measurable change was not found at high concentrations. Under stress conditions the antioxidant enzymes were up-regulated compared to control. An increase in protein content and enzyme activities was observed at moderate exposure conditions followed by a decline at both roots and leaves. The maximum enzyme activities were observed at different exposure conditions. Our results showed that N. officinale had the capacity to overcome nickel-induced stress especially at moderate nickel exposure. Therefore, N. officinale may be used as a phytoremediator in moderately polluted aquatic ecosystems.
基金supported by the National Natural Science Foundation of China(31830015,31901302).
文摘Background:Inputs of above-and belowground litter into forest soils are changing at an unprecedented rate due to continuing human disturbances and climate change.Microorganisms drive the soil carbon(C)cycle,but the roles of above-and belowground litter in regulating the soil microbial community have not been evaluated at a global scale.Methods:Here,we conducted a meta-analysis based on 68 aboveground litter removal and root exclusion studies across forest ecosystems to quantify the roles of above-and belowground litter on soil microbial community and compare their relative importance.Results:Aboveground litter removal significantly declined soil microbial biomass by 4.9%but root exclusion inhibited it stronger,up to 11.7%.Moreover,the aboveground litter removal significantly raised fungi by 10.1%without altering bacteria,leading to a 46.7%increase in the fungi-to-bacteria(F/B)ratio.Differently,root exclusion significantly decreased the fungi by 26.2%but increased the bacteria by 5.7%,causing a 13.3%decrease in the F/B ratio.Specifically,root exclusion significantly inhibited arbuscular mycorrhizal fungi,ectomycorrhizal fungi,and actinomycetes by 22.9%,43.8%,and 7.9%,respectively.The negative effects of aboveground litter removal on microbial biomass increased with mean annual temperature and precipitation,whereas that of root exclusion on microbial biomass did not change with climatic factors but amplified with treatment duration.More importantly,greater effects of root exclusion on microbial biomass than aboveground litter removal were consistent across diverse forest biomes(expect boreal forests)and durations.Conclusions:These data provide a global evidence that root litter inputs exert a larger control on microbial biomass than aboveground litter inputs in forest ecosystems.Our study also highlights that changes in above-and belowground litter inputs could alter soil C stability differently by shifting the microbial community structure in the opposite direction.These findings are useful for predicting microbe-mediated C processes in response to changes in forest management or climate.
基金the National Key R&D Program of China(2016YFD0101804-6)the National Natural Science Foundation of China(31671691)the International Science&Technology Cooperation Program of China(2016YFE0108600)。
文摘Plant nitrogen assimilation and use efficiency in the seedling's root system are beneficial for adult plants in field condition for yield enhancement.Identification of the genetic basis between root traits and N uptake plays a crucial role in wheat breeding.In the present study,198 doubled haploid lines from the cross of Yangmai 16/Zhongmai 895 were used to identify quantitative trait loci(QTLs)underpinning four seedling biomass traits and five root system architecture(RSA)related traits.The plants were grown under hydroponic conditions with control,low and high N treatments(Ca(NO_(3))_(2)·4H_(2)O at 0,0.05 and 2.0 mmol L^(-1),respectively).Significant variations among the treatments and genotypes,and positive correlations between seedling biomass and RSA traits(r=0.20 to 0.98)were observed.Inclusive composite interval mapping based on a high-density map from the Wheat 660 K single nucleotide polymorphisms(SNP)array identified 51 QTLs from the three N treatments.Twelve new QTLs detected on chromosomes 1 AL(1)in the control,1 DS(2)in high N treatment,4 BL(5)in low and high N treatments,and 7 DS(3)and 7 DL(1)in low N treatments,are first reported in influencing the root and biomass related traits for N uptake.The most stable QTLs(RRS.caas-4 DS)on chromosome 4 DS,which were related to ratio of root to shoot dry weight trait,was in close proximity of the Rht-D1 gene,and it showed high phenotypic effects,explaining 13.1%of the phenotypic variance.Twenty-eight QTLs were clustered in 12 genetic regions.SNP markers tightly linked to two important QTLs clusters C10 and C11 on chromosomes 6 BL and 7 BL were converted to kompetitive allele-specific PCR(KASP)assays that underpin important traits in root development,including root dry weight,root surface area and shoot dry weight.These QTLs,clusters and KASP assays can greatly improve the efficiency of selection for root traits in wheat breeding programmes.
基金the State Key Research Development Program of China(2016YFC0501803,2016YFC0501802)the China Postdoctoral Science Foundation(2017M620889)the Second Tibetan Plateau Scientific Expedition and Research(2019QZKK0405).
文摘Understanding the effects of degradation on belowground biomass(BGB)is essential for assessment of carbon budget of the alpine meadow ecosystem on the Tibetan Plateau,China.This ecosystem has been undergoing serious degradation owing to climate change and anthropogenic activities.This study examined the response of the vertical distribution of plant BGB to degradation and explored the underlying mechanisms in an alpine meadow on the Tibetan Plateau.A field survey was conducted in an alpine meadow with seven sequential degrees of degradation in the Zoige Plateau on the Tibetan Plateau during the peak growing season of 2018.We measured aboveground biomass(AGB),BGB,soil water content(SWC),soil bulk density(SBD),soil compaction(SCOM),soil organic carbon(SOC),soil total nitrogen(STN),soil total phosphorus(STP),soil available nitrogen(SAN),and soil available phosphorus(STP)in the 0-30 cm soil layers.Our results show that degradation dramatically decreased the BGB in the 0-10 cm soil layer(BGB0-10)but slightly increased the subsoil BGB.The main reason may be that the physical-chemical properties of surface soil were more sensitive to degradation than those of subsoil,as indicated by the remarked positive associations of the trade-off value of BGB0-10 with SWC,SCOM,SOC,STN,SAN,and STP,as well as the negative correlation between the trade-off value of BGB0-10 and SBD in the soil layer of 0-10 cm.In addition,an increase in the proportion of forbs with increasing degradation degree directly affected the BGB vertical distribution.The findings suggest that the decrease in the trade-off value of BGB0-10 in response to degradation might be an adaptive strategy for the degradation-induced drought and infertile soil conditions.This study can provide theoretical support for assessing the effects of degradation on the carbon budget and sustainable development in the alpine meadow ecosystem on the Tibetan Plateau as well as other similar ecosystems in the world.
文摘Descriptions of tree root morphology inform design of belowground biomass and carbon inventories and sampling for research. We studied root morphology of tanoak (Notholithocarpus densiflorus), an important component in mixed evergreen forests of California and Oregon, USA. Tanoak re-sprouts from belowground lignotubers after disturbances, and stores an unknown amount of carbon in coarse roots underground. We sought to ascribe explanatory nomenclature to roots’ morphological features and to identify models describing tanoak root morphology. Twelve tanoak root systems were excavated, dissected, and measured. Roots tapered according to their circumference and location. Larger roots closer to the lignotuber (located at the base of the tree stem) tapered more rapidly per unit of length. Tanoak roots forked frequently. Root cross-sectional area was preserved after forking events (i.e., the sum of cross-sectional areas for smaller roots on one side of the fork correlated with the adjoining large root). Occurrence and quantity of root branches (small roots branching laterally from larger roots) was dependent upon length of the source root segment. Our models of tanoak root morphology are designed to be organized together to estimate biomass of any segment or collection of lateral roots (e.g., roots lost/missed during excavation, or in lieu of destructive sampling), given root diameter at a known distance from the lignotuber. The taper model gives distal- and proximal-end diameters for calculation of volume for segments of root tapering between forks. Frequency of forking and branching can also be predicted. Summing the predicted mass of each lateral root segment, branch, and forked segment would produce an estimate of mass for a contiguous network of lateral roots.