Determining the microstructure in human cementum might help us design new kinds of replacement materials for the treatment of teeth injury and disease. The authors characterized the nanostructures in the cementum of h...Determining the microstructure in human cementum might help us design new kinds of replacement materials for the treatment of teeth injury and disease. The authors characterized the nanostructures in the cementum of health human teeth via scanning electronic microscopy（SEM）. It was found that the acellular cementum is mainly composed of two kinds of nanostructures―inorganic nanoparticles and organic nanofibers. And the inorganic nanoparticles show a tendency to arrange along the organic nanofibers. Based on the micro-molding in capillary strategy, the distribution of organic component in acellular cementum was copied with UV curable resin. After removing the inorganic nanoparticles by acid etching, many isolated spindle shape nanopores were left in polymer, which su- ggested that the inorganic nanoparticles should have been isolated by the organic component in cementum, and should be oval or nanosheet in shape. We hope the present work could provide reference for the biomimetic preparation of tissue engineering materials, and help us design new types of tooth implant.展开更多
目的研究根管预备、充填及纤维桩预备对根管壁微裂产生的影响。方法收集150颗离体前磨牙,并随机分为四个大组[1组(n=10)、2组(n=20)、3组(n=40)、4组(n=80)]及15个小组(1组,2组25#、2组40#,3组A25#、3组B25#、3组A40#、3组B40#,4组AX25#...目的研究根管预备、充填及纤维桩预备对根管壁微裂产生的影响。方法收集150颗离体前磨牙,并随机分为四个大组[1组(n=10)、2组(n=20)、3组(n=40)、4组(n=80)]及15个小组(1组,2组25#、2组40#,3组A25#、3组B25#、3组A40#、3组B40#,4组AX25#、4组AX40#、4组AT25#、4组AT40#、4组BX25#、4组BX40#、4组BT25#、4组BT40#,n=10)。1组:即对照组,不进行预备;2组:均分为两组,一组预备至WaveOne25#,另一组预备则预备至40#;3组:将样本与2组一样进行分组预备,之后进一步分为2个小组,进行冷牙胶侧压充填(A)及热牙胶连续波充填(B);4组:将样本与3组一样进行分组预备及充填,之后将不同方法充填后的样本进一步分为2小组进行RelyXTM(X)和RTD(T)纤维桩预备。将所有的样本从距离根尖3、5、7 mm处垂直于牙体长轴截断,在显微镜下观察微裂发生情况,并进行比较分析。结果1组及2组中均并未有裂纹存在。充填后,3组A与3组B的裂纹数目比较差异无统计学意义(P>0.05)。置入纤维桩后,4组A的牙本质裂纹明显较4组B多,且差异具有统计学意义(22/120 VS 10/120,P<0.05)。在与根尖距离7 mm处,相较于RelyXTM纤维桩,RTD纤维桩预备后牙本质产生更多裂纹,差异具有统计学意义(9/120 VS 23/120,P<0.05)。4组的裂纹总发生率高于1组、2组、3组(P<0.05),说明纤维桩置入后,牙本质裂纹较仅进行牙体预备和充填明显增多。结论纤维桩的预备主要导致根管壁裂纹的产生,冷侧压充填后进行纤维桩预备比热牙胶充填后进行桩体预备更容易产生根管壁裂纹。展开更多
Purpose: The aim of this systematic review was to answer the question “Which treatment protocol, am- ong classical methods and/or various laser applica- tions is the most effective in root canal disinfection, in vitr...Purpose: The aim of this systematic review was to answer the question “Which treatment protocol, am- ong classical methods and/or various laser applica- tions is the most effective in root canal disinfection, in vitro”. Materials and Methods: A MEDLINE, a Co- chrane and an Embase search (three specified search- es) were conducted to identify randomized controlled trials (RCT) until June 2010, conducted on human teeth and published in English, German or French language, examining the root canal disinfection after the use of lasers with or without mechanical instru-mentation. Additionally, hand search was conducted and contact with authors, when needed. Results: The MEDLINE, the Cochrane and the EMBASE search identified 240, 28, and 35 published articles, respec-tively. Ten articles from the MEDLINE and 5 articles from the Cochrane search (that were also identified in the MEDLINE search) met the inclusion and va-lidity assessment criteria. In E. faecalis elimination, instrumentation of the root canal and diode laser/665 nanometer/1 Watt (diode laser/665 nm/1 W) irradia-tion with the combined effect of Methylene Blue (MB) as photosensitizing agent (logCFU/ml = 1636) seemed to be the best method. In P. aeruginosa and in A.naeslundii elimination, instrumentation of the root canal followed by irrigation with 5, 25% NaOCl (log-CFU/ml = 0) seemed to be the best method. In gen-eral, instrumentation of the root canal followed by irrigation with 5, 25% NaOCl (logCFU/ml = 0) and instrumentation of the root canal and Er: YAG laser/ 2940 nm/0.8 W irradiation (logCFU/ml = 1924) seemed to be the best (polymicrobial studies). Conclusions: There are treatment protocols with the assistance or not of laser irradiation that can eliminate E. faecalis, E. coli and S. aureus inside the root canal. However, there is a serious number of S. anginosus, F. nuclea-tum, A. naeslundii and P. aeruginosa that remain in-side the root canal even after laser irradiation. New research is needed in order to set a treatment proto-col effective in the root canal disinfection from all bac-teria that are related to endodontic origin pathology.展开更多
基金Supported by the National Natural Science Foundation of China(No.30830108)the National Basic Research Program of China(No.2007CB936402)
文摘Determining the microstructure in human cementum might help us design new kinds of replacement materials for the treatment of teeth injury and disease. The authors characterized the nanostructures in the cementum of health human teeth via scanning electronic microscopy（SEM）. It was found that the acellular cementum is mainly composed of two kinds of nanostructures―inorganic nanoparticles and organic nanofibers. And the inorganic nanoparticles show a tendency to arrange along the organic nanofibers. Based on the micro-molding in capillary strategy, the distribution of organic component in acellular cementum was copied with UV curable resin. After removing the inorganic nanoparticles by acid etching, many isolated spindle shape nanopores were left in polymer, which su- ggested that the inorganic nanoparticles should have been isolated by the organic component in cementum, and should be oval or nanosheet in shape. We hope the present work could provide reference for the biomimetic preparation of tissue engineering materials, and help us design new types of tooth implant.
文摘目的研究根管预备、充填及纤维桩预备对根管壁微裂产生的影响。方法收集150颗离体前磨牙,并随机分为四个大组[1组(n=10)、2组(n=20)、3组(n=40)、4组(n=80)]及15个小组(1组,2组25#、2组40#,3组A25#、3组B25#、3组A40#、3组B40#,4组AX25#、4组AX40#、4组AT25#、4组AT40#、4组BX25#、4组BX40#、4组BT25#、4组BT40#,n=10)。1组:即对照组,不进行预备;2组:均分为两组,一组预备至WaveOne25#,另一组预备则预备至40#;3组:将样本与2组一样进行分组预备,之后进一步分为2个小组,进行冷牙胶侧压充填(A)及热牙胶连续波充填(B);4组:将样本与3组一样进行分组预备及充填,之后将不同方法充填后的样本进一步分为2小组进行RelyXTM(X)和RTD(T)纤维桩预备。将所有的样本从距离根尖3、5、7 mm处垂直于牙体长轴截断,在显微镜下观察微裂发生情况,并进行比较分析。结果1组及2组中均并未有裂纹存在。充填后,3组A与3组B的裂纹数目比较差异无统计学意义(P>0.05)。置入纤维桩后,4组A的牙本质裂纹明显较4组B多,且差异具有统计学意义(22/120 VS 10/120,P<0.05)。在与根尖距离7 mm处,相较于RelyXTM纤维桩,RTD纤维桩预备后牙本质产生更多裂纹,差异具有统计学意义(9/120 VS 23/120,P<0.05)。4组的裂纹总发生率高于1组、2组、3组(P<0.05),说明纤维桩置入后,牙本质裂纹较仅进行牙体预备和充填明显增多。结论纤维桩的预备主要导致根管壁裂纹的产生,冷侧压充填后进行纤维桩预备比热牙胶充填后进行桩体预备更容易产生根管壁裂纹。
文摘Purpose: The aim of this systematic review was to answer the question “Which treatment protocol, am- ong classical methods and/or various laser applica- tions is the most effective in root canal disinfection, in vitro”. Materials and Methods: A MEDLINE, a Co- chrane and an Embase search (three specified search- es) were conducted to identify randomized controlled trials (RCT) until June 2010, conducted on human teeth and published in English, German or French language, examining the root canal disinfection after the use of lasers with or without mechanical instru-mentation. Additionally, hand search was conducted and contact with authors, when needed. Results: The MEDLINE, the Cochrane and the EMBASE search identified 240, 28, and 35 published articles, respec-tively. Ten articles from the MEDLINE and 5 articles from the Cochrane search (that were also identified in the MEDLINE search) met the inclusion and va-lidity assessment criteria. In E. faecalis elimination, instrumentation of the root canal and diode laser/665 nanometer/1 Watt (diode laser/665 nm/1 W) irradia-tion with the combined effect of Methylene Blue (MB) as photosensitizing agent (logCFU/ml = 1636) seemed to be the best method. In P. aeruginosa and in A.naeslundii elimination, instrumentation of the root canal followed by irrigation with 5, 25% NaOCl (log-CFU/ml = 0) seemed to be the best method. In gen-eral, instrumentation of the root canal followed by irrigation with 5, 25% NaOCl (logCFU/ml = 0) and instrumentation of the root canal and Er: YAG laser/ 2940 nm/0.8 W irradiation (logCFU/ml = 1924) seemed to be the best (polymicrobial studies). Conclusions: There are treatment protocols with the assistance or not of laser irradiation that can eliminate E. faecalis, E. coli and S. aureus inside the root canal. However, there is a serious number of S. anginosus, F. nuclea-tum, A. naeslundii and P. aeruginosa that remain in-side the root canal even after laser irradiation. New research is needed in order to set a treatment proto-col effective in the root canal disinfection from all bac-teria that are related to endodontic origin pathology.
