Summary： This study was aimed to evaluate the effectiveness of solution form of 17% ethylenediaminetetraacetic acid （EDTA） on removing smear layer of root canals at different exposure time periods and to provide sc...Summary： This study was aimed to evaluate the effectiveness of solution form of 17% ethylenediaminetetraacetic acid （EDTA） on removing smear layer of root canals at different exposure time periods and to provide scientific basis for EDTA as a choice of root canal irrigation in clinical practice. Twenty-five single-rooted teeth were randomly divided into 5 groups： control group （group A） was given 2.5% NaOC1, and 4 experimental groups were given 2.5% NaOC1 and 17% EDTA, including groups B, C, D and E with exposure time of 1, 3, 5 and 7 min, respectively. After preparation of the root canals, the teeth were split along their longitudinal axis, and the root sections were examined under scanning elec- tron microscope for evaluation of smear layer removal and erosion on the surface of the root canal walls. The specimens in group B showed presence of smear layer on the walls of the root canal with no statistical difference from that in group A （P〉0.05）. In groups C and D, partial removal of smear layer was obtained, and there was no significant difference between the two groups （P〉0.05）, but there was significant difference in removal of smear layer between group C and group B （P〈0.05）. Root canal walls in group E specimens showed almost complete removal of smear layer, and the removal of smear layer was significantly different from that in group D （P〈0.01）. There was no significant change in the structure of the surface of root canal for each sample. It was concluded that combined irrigation with 17% EDTA and 2.5% NaOC1 could remove the smear layer with no significant alteration in dentinal structure when the chelating agent was applied for 7 min. At 3 and 5 min of application, partial removal of smear layer was observed and at 1 min negligible removal of smear layer was achieved.展开更多
Deep phosphorus application can be a usefull measure to improve crops＇ performance in semi-arid regions, but more knowledge of both its general effects and effects on specific crops is required to optimize treatments...Deep phosphorus application can be a usefull measure to improve crops＇ performance in semi-arid regions, but more knowledge of both its general effects and effects on specific crops is required to optimize treatments. Thus, the aims of this study were to evaluate the effects of phosphorus（P） application at different soil layers on root growth, grain yield, and water-use efficiency（WUE） of winter wheat grown on the semi-arid Loess Plateau of China and to explore the relationship between root distribution and grain yield. The experiment consisted of four P treatments in a randomized complete block design with three replicates and two cultivars： one drought-sensitive（Xiaoyan 22, XY22） and one drought-tolerant（Changhan 58, CH58）. The four P treatments were no P（control, CK）, surface P（SP）, deep P（DP）, and deep-band P application（DBP）. CH58 produced larger and deeper root systems, and had higher grain yields and WUE, under the deep P treatments（DP and DBP） than under SP, clearly showing that deep P placement had beneficial effects on the drought-tolerant cultivar. In contrast, the grain yield and root growth of XY22 did not differ between DP or DBP and SP treatments. Further, root dry weight（RW） and root length（RL） in deep soil layer（30-100 cm） were closely positively correlated with grain yield and WUE of CH58（but not XY22）, highlighting the connections between a well-developed subsoil root system and both high grain yield and WUE for the drought-tolerant cultivar. WUE correlated strongly with grain yield for both cultivars（r=0.94, P〈0.001）. In conclusion, deep application of P fertilizer is a practical and feasible means of increasing grain yield and WUE of rainfed winter wheat in semi-arid regions, by promoting deep root development of drought-tolerant cultivars.展开更多
Shea tree (Vitellaria paradoxa Gaertn. Family;Sapotaceae) indigenous to the Sudano-sahelian zone of Africa has great economic and ecological potential and attributes. Commercial cultivation of the tree is however, ham...Shea tree (Vitellaria paradoxa Gaertn. Family;Sapotaceae) indigenous to the Sudano-sahelian zone of Africa has great economic and ecological potential and attributes. Commercial cultivation of the tree is however, hampered by the poor rooting success of its planting material with adverse consequences on field establishment and total fruit yield. A 3 × 2 factorial experiment arranged in a randomized complete block design was carried out in 2012 at Bole in the Interior Savanna agro-ecological zone. The objective was to assess the rooting success of Shea shoots by the air-layering technique using two media types (palm fibre and Sphagnum moss) and three IBA hormone concentrations (0, 5000 and 10,000 ppm) under contrasting climatic (wet and dry) conditions. Layered shoots which were sprayed with 10,000 ppm IBA and wrapped with Sphagnum moss gave significantly (P < 0.05) higher rooting success in terms of more roots per cutting (73.3%) and longer roots per cutting (9.0 cm) than palm fibre at both 5000 ppm (30.0%;3.7 cm) and 10,000 ppm (46.7%;7.9 cm) concentrations. Higher rooting success was significantly achieved in the wet season than in the dry season. Sphagnum moss treated with 10,000 ppm IBA facilitated the translocation of higher levels of sugar and total free phenol (TFP) to the base of the layered shoots which resulted in significantly (P < 0.05) higher number of roots and better roots protection from fungal infection than the other treatments. Rooting of layered shoots was affected to a greater extent by low temperatures (Rooting = 836 – 34.2 Temp (low);R2 = 82%, p < 0.001) than by high temperatures (Rooting = 5250 – 175.0 Temp (high);R2 = 64.5%, p < 0.009). Rooting of layered shoots was also significantly and negatively affected by the spread of the canopy of the selected tree such that closed canopy trees resulted in higher and better rooting that open canopy trees (Rooting = 113.87-23.697(Canopy spread);R2 = 89%, p = 0.002;n = 9). Furthermore, significant and positive correlations were found between IBA concentration level and simple sugars (r = 0.99;P < 0.0001;n = 9) and also between IBA concentration level and total free phenols (r = 0.98;P < 0.0002;n = 9). The study concluded that to achieve high rooting success in the propagation of Shea nut tree by the air-layering technique, a combination of Sphagnum moss treated with 10,000 ppm of IBA hormone should be used and the whole set up carried out in the wet season.展开更多
目的:建立苗药紫藤根药材超高效液相色谱法(UPLC)指纹图谱及4个黄酮类成分含量测定方法,为全面评价其质量提供依据。方法:采用UPLC建立紫藤根药材指纹图谱,对不同产地药材进行相似度评价、聚类分析和主成分分析;同时对4个黄酮类成分含...目的:建立苗药紫藤根药材超高效液相色谱法(UPLC)指纹图谱及4个黄酮类成分含量测定方法,为全面评价其质量提供依据。方法:采用UPLC建立紫藤根药材指纹图谱,对不同产地药材进行相似度评价、聚类分析和主成分分析;同时对4个黄酮类成分含量进行测定。结果:建立了紫藤根UPLC指纹图谱,确定19个共有峰,指认了其中4个成分,分别为鸢尾苷、芒柄花苷、紫藤苷、biochanin A 7-O-glucoside。12批紫藤根药材相似度介于0.839至0.975之间;聚类分析可将不同产地的药材分为3类,表明不同产地药材存在一定差异;含量测定结果显示,安徽亳州产地药材鸢尾苷含量最高、湖南湘阴产地药材芒柄花苷含量最高,两地4个指标性成分总含量较高且稳定。结论:该方法可以有效评价不同产区紫藤根药材的质量差异性,为其质量控制和药材选购提供参考。展开更多
基金supported by the Fundamental Research Funds for the Central Universities,China(No.2010JC030)
文摘Summary： This study was aimed to evaluate the effectiveness of solution form of 17% ethylenediaminetetraacetic acid （EDTA） on removing smear layer of root canals at different exposure time periods and to provide scientific basis for EDTA as a choice of root canal irrigation in clinical practice. Twenty-five single-rooted teeth were randomly divided into 5 groups： control group （group A） was given 2.5% NaOC1, and 4 experimental groups were given 2.5% NaOC1 and 17% EDTA, including groups B, C, D and E with exposure time of 1, 3, 5 and 7 min, respectively. After preparation of the root canals, the teeth were split along their longitudinal axis, and the root sections were examined under scanning elec- tron microscope for evaluation of smear layer removal and erosion on the surface of the root canal walls. The specimens in group B showed presence of smear layer on the walls of the root canal with no statistical difference from that in group A （P〉0.05）. In groups C and D, partial removal of smear layer was obtained, and there was no significant difference between the two groups （P〉0.05）, but there was significant difference in removal of smear layer between group C and group B （P〈0.05）. Root canal walls in group E specimens showed almost complete removal of smear layer, and the removal of smear layer was significantly different from that in group D （P〈0.01）. There was no significant change in the structure of the surface of root canal for each sample. It was concluded that combined irrigation with 17% EDTA and 2.5% NaOC1 could remove the smear layer with no significant alteration in dentinal structure when the chelating agent was applied for 7 min. At 3 and 5 min of application, partial removal of smear layer was observed and at 1 min negligible removal of smear layer was achieved.
基金supported by the National Natural Science Foundation of China(31270553)the National 973 Program of China(2009CB118604)the Special Fund for Agro-Scientific Research in the Public Interest of China(201103003)
文摘Deep phosphorus application can be a usefull measure to improve crops＇ performance in semi-arid regions, but more knowledge of both its general effects and effects on specific crops is required to optimize treatments. Thus, the aims of this study were to evaluate the effects of phosphorus（P） application at different soil layers on root growth, grain yield, and water-use efficiency（WUE） of winter wheat grown on the semi-arid Loess Plateau of China and to explore the relationship between root distribution and grain yield. The experiment consisted of four P treatments in a randomized complete block design with three replicates and two cultivars： one drought-sensitive（Xiaoyan 22, XY22） and one drought-tolerant（Changhan 58, CH58）. The four P treatments were no P（control, CK）, surface P（SP）, deep P（DP）, and deep-band P application（DBP）. CH58 produced larger and deeper root systems, and had higher grain yields and WUE, under the deep P treatments（DP and DBP） than under SP, clearly showing that deep P placement had beneficial effects on the drought-tolerant cultivar. In contrast, the grain yield and root growth of XY22 did not differ between DP or DBP and SP treatments. Further, root dry weight（RW） and root length（RL） in deep soil layer（30-100 cm） were closely positively correlated with grain yield and WUE of CH58（but not XY22）, highlighting the connections between a well-developed subsoil root system and both high grain yield and WUE for the drought-tolerant cultivar. WUE correlated strongly with grain yield for both cultivars（r=0.94, P〈0.001）. In conclusion, deep application of P fertilizer is a practical and feasible means of increasing grain yield and WUE of rainfed winter wheat in semi-arid regions, by promoting deep root development of drought-tolerant cultivars.
文摘Shea tree (Vitellaria paradoxa Gaertn. Family;Sapotaceae) indigenous to the Sudano-sahelian zone of Africa has great economic and ecological potential and attributes. Commercial cultivation of the tree is however, hampered by the poor rooting success of its planting material with adverse consequences on field establishment and total fruit yield. A 3 × 2 factorial experiment arranged in a randomized complete block design was carried out in 2012 at Bole in the Interior Savanna agro-ecological zone. The objective was to assess the rooting success of Shea shoots by the air-layering technique using two media types (palm fibre and Sphagnum moss) and three IBA hormone concentrations (0, 5000 and 10,000 ppm) under contrasting climatic (wet and dry) conditions. Layered shoots which were sprayed with 10,000 ppm IBA and wrapped with Sphagnum moss gave significantly (P < 0.05) higher rooting success in terms of more roots per cutting (73.3%) and longer roots per cutting (9.0 cm) than palm fibre at both 5000 ppm (30.0%;3.7 cm) and 10,000 ppm (46.7%;7.9 cm) concentrations. Higher rooting success was significantly achieved in the wet season than in the dry season. Sphagnum moss treated with 10,000 ppm IBA facilitated the translocation of higher levels of sugar and total free phenol (TFP) to the base of the layered shoots which resulted in significantly (P < 0.05) higher number of roots and better roots protection from fungal infection than the other treatments. Rooting of layered shoots was affected to a greater extent by low temperatures (Rooting = 836 – 34.2 Temp (low);R2 = 82%, p < 0.001) than by high temperatures (Rooting = 5250 – 175.0 Temp (high);R2 = 64.5%, p < 0.009). Rooting of layered shoots was also significantly and negatively affected by the spread of the canopy of the selected tree such that closed canopy trees resulted in higher and better rooting that open canopy trees (Rooting = 113.87-23.697(Canopy spread);R2 = 89%, p = 0.002;n = 9). Furthermore, significant and positive correlations were found between IBA concentration level and simple sugars (r = 0.99;P < 0.0001;n = 9) and also between IBA concentration level and total free phenols (r = 0.98;P < 0.0002;n = 9). The study concluded that to achieve high rooting success in the propagation of Shea nut tree by the air-layering technique, a combination of Sphagnum moss treated with 10,000 ppm of IBA hormone should be used and the whole set up carried out in the wet season.
文摘目的:建立苗药紫藤根药材超高效液相色谱法(UPLC)指纹图谱及4个黄酮类成分含量测定方法,为全面评价其质量提供依据。方法:采用UPLC建立紫藤根药材指纹图谱,对不同产地药材进行相似度评价、聚类分析和主成分分析;同时对4个黄酮类成分含量进行测定。结果:建立了紫藤根UPLC指纹图谱,确定19个共有峰,指认了其中4个成分,分别为鸢尾苷、芒柄花苷、紫藤苷、biochanin A 7-O-glucoside。12批紫藤根药材相似度介于0.839至0.975之间;聚类分析可将不同产地的药材分为3类,表明不同产地药材存在一定差异;含量测定结果显示,安徽亳州产地药材鸢尾苷含量最高、湖南湘阴产地药材芒柄花苷含量最高,两地4个指标性成分总含量较高且稳定。结论:该方法可以有效评价不同产区紫藤根药材的质量差异性,为其质量控制和药材选购提供参考。
