目的比较生物陶瓷材料iRoot BP Plus和无机三氧化物聚合物(MTA)形成根尖屏障修复根尖未发育完全恒牙的效果。方法回顾2018年1月至2022年8月医院牙体牙髓专家门诊收治的80例(80颗患牙)根尖未发育完全恒牙患者,按材料差异应用差异分为两...目的比较生物陶瓷材料iRoot BP Plus和无机三氧化物聚合物(MTA)形成根尖屏障修复根尖未发育完全恒牙的效果。方法回顾2018年1月至2022年8月医院牙体牙髓专家门诊收治的80例(80颗患牙)根尖未发育完全恒牙患者,按材料差异应用差异分为两组,A组40例:根尖屏障修复术中应用iRoot BP Plus材料;B组40例:术中应用MTA材料;对比两组修复效果。结果A组、B组效果评价比较(P>0.05)。A组充填操作时间(8.51±1.38)min,短于B组的(15.48±2.09)min,差异有统计学意义(P<0.05)。A组治疗6个月O-PAI评分低于B组,并发症发生率2.50%低于B组的20.00%(P<0.05)。结论iRoot BP Plus和MTA形成根尖屏障修复根尖未发育完全恒牙均有相似效果,但iRoot BP Plus充填时间短,并发症少,值得应用。展开更多
The reorientation of microtubules (MTS) in roots of Oryza sativa L. treated with 2,4_D was studied using confocal laser scanning microscopy. In the control (the roots were not treated with 2,4_D), different distributi...The reorientation of microtubules (MTS) in roots of Oryza sativa L. treated with 2,4_D was studied using confocal laser scanning microscopy. In the control (the roots were not treated with 2,4_D), different distribution patterns of MTS were observed in the different growth zones of root tips. MTS of the cortical cells were randomly aligned in the zone of cell division. They were transversely arranged in the cortical cells of the zone of cell elongation; and obliquely oriented in the root hair zone. After treatment with 2,4_D, MTS displayed distinct changes with reorientation in the cortex of the root tip coupling with the inhibition of root growth. MTS changed their orientation in the cortical cells of the zone of cell division from being randomly oriented to transversely oriented when incubated in 1 mg/L 2,4_D for 1 h. However, they were recovered and became randomly oriented when the roots were treated with 1 mg/L 2,4_D for 24 h. An array of MTS, which was different from that in the control, was observed in the cortical cells of the zone of cell elongation in the roots treated with 1 mg/L 2,4_D for 1 h. After treatment with 10 mg/L 2,4_D for 1 h, the MTS in the cortical cells of the zone of cell division became transversely reorientated, but the pattern of MTS distribution was different from that in the 1_hour treatment of 1 mg/L 2,4_D. MTS were hardly detected in the same type of cells when roots were submerged in 10 mg/L 2,4_D for 24 h. When roots were incubated in 10 mg/L 2,4_D for 1 h, the MTS of the cortical cells in the zone of cell elongation became randomly oriented, and much more randomly when the roots were treated for 24 h.展开更多
ObjectiveThe aim was to assess genetic and physiological toxicity of wastewater from a pharmaceutical factory using root tip micronucleus technology of Vicia faba. MethodThe pollution of wastewater from a pharmaceutic...ObjectiveThe aim was to assess genetic and physiological toxicity of wastewater from a pharmaceutical factory using root tip micronucleus technology of Vicia faba. MethodThe pollution of wastewater from a pharmaceutical factory was detected by using root tip micronucleus technology of Vicia faba, and the genetic and physiological toxicity of the wastewater to Vicia faba was assessed. ResultNon-processed wastewater had an extremely high level of biological toxicity; the cells were unable to live with the wastewater at a high concentration; the cells were able to grow with the wastewater at a low concentration, though the micronucleus ratio was extremely high. The processed wastewater had no significant impact on cell growth, but the micronucleus ratio was extremely high, showing that the processed water also had a high pollution index. ConclusionThe research could provide scientific references for the national treatment of wastewater from a pharmaceutical factory.展开更多
BACKGROUND Several previous studies have reported an unusual root formation in which a fractured apical fragment of an immature root continued to develop independent of the main root after trauma to an immature tooth....BACKGROUND Several previous studies have reported an unusual root formation in which a fractured apical fragment of an immature root continued to develop independent of the main root after trauma to an immature tooth.To date,there have been only rare reports of the continuing apical formation of the fractured root associated with dens evaginatus(DE).This paper presents a case of a separated root tip formation associated with a fractured tubercle of DE.CASE SUMMARY An 11-year-old boy was referred for gingival sinus on the buccal side of the right mandibular second premolar(tooth#45).Clinically,tooth#45 was free of caries,but there was a sign of a fractured tubercle of DE on the occlusal surface.Radiography showed that the root canal of tooth#45 was widely radiolucent.A separated root apex was found apically under the main root and was nearly completely formed with an apical orifice at the apical tip.Tooth#45 was diagnosed as tubular fracture of DE with chronic apical periodontitis.A revascularization technique was recommended to treat the tooth.At 3-mo and 1-yr follow-up,the patient remained asymptomatic.Periapical radiography revealed that the separated root tip distally drifted with closure of the apex.However,the root length and thickness of the main root did not increased.CONCLUSION Clinicians should be aware that even if tubercle of DE is fractured in an immature tooth,the root tip may be separated from the main root and completely formed.展开更多
[Objective] This study aimed to establish an effective detection technology for rapidly identifying Bt transgenic sugarcane, promptly removing non-Bt transgenic plants and increasing breeding efficiency of Bt transgen...[Objective] This study aimed to establish an effective detection technology for rapidly identifying Bt transgenic sugarcane, promptly removing non-Bt transgenic plants and increasing breeding efficiency of Bt transgenic plants, thereby improving breeding efficiency of insect-resistant sugarcane cultivars in China. [ Method] Approximately 1 mg of root tips and other materials were crushed, placed in the bottom of PCR tubes, added successively with solution I and mineral oil, headed at 95 ℃ for 15 rain, added with solution Ⅱ, and finally added with solution III containing GBt primer for PCR. After separated by using electrophoresis on agarese gel, amplification products were observed and photographed under a gel imaging system. [ Result] Various materials treated with different concentrations of solution led to different amplification results. Specifically, the appropriate concentration of NaOH in solution I was 0.1 -0.2 mol/L; the appropriate pH value of solution II was 2. No bands were amplified from mature leaves, tender leaves and old roots of Bt transgenic sugarcane, while root tips successfully amplified 545 bp target bands. The established method was used to identify and analyze 63 hybrid seedlings of YT 91 -976 × SBR216, results showed that a total of 17 samples exhibited 545 bp bands, accounting for approximately 27% of the total number of hybrid seedlings, indicating that these 17 seedlings were Bt transgenic sugarcane plants. [ Conclusion] The rapid detection method established in this study is conducive to identifying Bt transgenic sugarcane and improving breeding efficiency of insect-resistant sugarcane lines, which provides basis for screening of other transgenic materials.展开更多
Seeds of japonica rice cv.Zhenuo 2 with twodifferent physiological states(dry seeds withwater content 13% and wet seeds soaked in thewater for 36 h)were irradiated by COlaser infour different power-densities and durat...Seeds of japonica rice cv.Zhenuo 2 with twodifferent physiological states(dry seeds withwater content 13% and wet seeds soaked in thewater for 36 h)were irradiated by COlaser infour different power-densities and durations re-spectively.The treatment irradiated with 200GY ofCo γ-rays was considered as control.The flesh root tips were cut and fixed inCarnoy’s fluid for cytological examination.展开更多
西南冬麦区气候冬干春旱频发、土壤速效磷缺乏,限制冬小麦氮素吸收。本研究探究秋闲期秸秆覆盖与施磷对小麦根系NO3-吸收动力势、氮素吸收利用、叶绿素含量和籽粒产量的影响,以期为小麦高产稳产及养分的高效利用提供理论依据。试验于202...西南冬麦区气候冬干春旱频发、土壤速效磷缺乏,限制冬小麦氮素吸收。本研究探究秋闲期秸秆覆盖与施磷对小麦根系NO3-吸收动力势、氮素吸收利用、叶绿素含量和籽粒产量的影响,以期为小麦高产稳产及养分的高效利用提供理论依据。试验于2020—2022年在四川仁寿进行,采用二因素裂区设计,以秸秆覆盖(SM)和不覆盖(NSM)为主区;3个磷水平0(P0)、75(P75)和120(P120) kg hm-2为裂区。结果表明:秸秆覆盖与施磷显著提高地上部磷素积累量, SM较NSM的小麦根尖NO3-净吸收速率、籽粒氮积累量、氮素转运量、氮素同化量、氮肥偏生产力和籽粒产量分别增加28.2%、8.4%、9.0%、41.9%、23.3%和21.9%。与P0相比, P75和P120增加幅度分别达到35.1%~37.6%、12.6%~19.0%、7.1%~9.3%、35.7%~60.5%、17.6%~23.8%、17.2%~23.6%。与NSM相比, SM的小麦旗叶灌浆期叶绿素含量上升,进而提高籽粒产量。综上所述,秸秆覆盖与施磷可促进小麦根尖NO3-吸收,提高叶绿素含量,从而显著增加花后氮素的吸收及营养器官临时贮存氮素向籽粒的再分配,最终提高籽粒产量。考虑经济效益和产量回报,西南地区小麦高产高效栽培时,推荐采用秋闲期秸秆覆盖配施磷肥75 kg hm-2。展开更多
文摘目的比较生物陶瓷材料iRoot BP Plus和无机三氧化物聚合物(MTA)形成根尖屏障修复根尖未发育完全恒牙的效果。方法回顾2018年1月至2022年8月医院牙体牙髓专家门诊收治的80例(80颗患牙)根尖未发育完全恒牙患者,按材料差异应用差异分为两组,A组40例:根尖屏障修复术中应用iRoot BP Plus材料;B组40例:术中应用MTA材料;对比两组修复效果。结果A组、B组效果评价比较(P>0.05)。A组充填操作时间(8.51±1.38)min,短于B组的(15.48±2.09)min,差异有统计学意义(P<0.05)。A组治疗6个月O-PAI评分低于B组,并发症发生率2.50%低于B组的20.00%(P<0.05)。结论iRoot BP Plus和MTA形成根尖屏障修复根尖未发育完全恒牙均有相似效果,但iRoot BP Plus充填时间短,并发症少,值得应用。
文摘The reorientation of microtubules (MTS) in roots of Oryza sativa L. treated with 2,4_D was studied using confocal laser scanning microscopy. In the control (the roots were not treated with 2,4_D), different distribution patterns of MTS were observed in the different growth zones of root tips. MTS of the cortical cells were randomly aligned in the zone of cell division. They were transversely arranged in the cortical cells of the zone of cell elongation; and obliquely oriented in the root hair zone. After treatment with 2,4_D, MTS displayed distinct changes with reorientation in the cortex of the root tip coupling with the inhibition of root growth. MTS changed their orientation in the cortical cells of the zone of cell division from being randomly oriented to transversely oriented when incubated in 1 mg/L 2,4_D for 1 h. However, they were recovered and became randomly oriented when the roots were treated with 1 mg/L 2,4_D for 24 h. An array of MTS, which was different from that in the control, was observed in the cortical cells of the zone of cell elongation in the roots treated with 1 mg/L 2,4_D for 1 h. After treatment with 10 mg/L 2,4_D for 1 h, the MTS in the cortical cells of the zone of cell division became transversely reorientated, but the pattern of MTS distribution was different from that in the 1_hour treatment of 1 mg/L 2,4_D. MTS were hardly detected in the same type of cells when roots were submerged in 10 mg/L 2,4_D for 24 h. When roots were incubated in 10 mg/L 2,4_D for 1 h, the MTS of the cortical cells in the zone of cell elongation became randomly oriented, and much more randomly when the roots were treated for 24 h.
文摘ObjectiveThe aim was to assess genetic and physiological toxicity of wastewater from a pharmaceutical factory using root tip micronucleus technology of Vicia faba. MethodThe pollution of wastewater from a pharmaceutical factory was detected by using root tip micronucleus technology of Vicia faba, and the genetic and physiological toxicity of the wastewater to Vicia faba was assessed. ResultNon-processed wastewater had an extremely high level of biological toxicity; the cells were unable to live with the wastewater at a high concentration; the cells were able to grow with the wastewater at a low concentration, though the micronucleus ratio was extremely high. The processed wastewater had no significant impact on cell growth, but the micronucleus ratio was extremely high, showing that the processed water also had a high pollution index. ConclusionThe research could provide scientific references for the national treatment of wastewater from a pharmaceutical factory.
基金National Natural Science Foundation of China,No.81801028Natural Science Foundation of Zhejiang Province,No.LQ19H140001.
文摘BACKGROUND Several previous studies have reported an unusual root formation in which a fractured apical fragment of an immature root continued to develop independent of the main root after trauma to an immature tooth.To date,there have been only rare reports of the continuing apical formation of the fractured root associated with dens evaginatus(DE).This paper presents a case of a separated root tip formation associated with a fractured tubercle of DE.CASE SUMMARY An 11-year-old boy was referred for gingival sinus on the buccal side of the right mandibular second premolar(tooth#45).Clinically,tooth#45 was free of caries,but there was a sign of a fractured tubercle of DE on the occlusal surface.Radiography showed that the root canal of tooth#45 was widely radiolucent.A separated root apex was found apically under the main root and was nearly completely formed with an apical orifice at the apical tip.Tooth#45 was diagnosed as tubular fracture of DE with chronic apical periodontitis.A revascularization technique was recommended to treat the tooth.At 3-mo and 1-yr follow-up,the patient remained asymptomatic.Periapical radiography revealed that the separated root tip distally drifted with closure of the apex.However,the root length and thickness of the main root did not increased.CONCLUSION Clinicians should be aware that even if tubercle of DE is fractured in an immature tooth,the root tip may be separated from the main root and completely formed.
基金Supported by Modern Agricultural Industry Technology System (CARS-2004B)
文摘[Objective] This study aimed to establish an effective detection technology for rapidly identifying Bt transgenic sugarcane, promptly removing non-Bt transgenic plants and increasing breeding efficiency of Bt transgenic plants, thereby improving breeding efficiency of insect-resistant sugarcane cultivars in China. [ Method] Approximately 1 mg of root tips and other materials were crushed, placed in the bottom of PCR tubes, added successively with solution I and mineral oil, headed at 95 ℃ for 15 rain, added with solution Ⅱ, and finally added with solution III containing GBt primer for PCR. After separated by using electrophoresis on agarese gel, amplification products were observed and photographed under a gel imaging system. [ Result] Various materials treated with different concentrations of solution led to different amplification results. Specifically, the appropriate concentration of NaOH in solution I was 0.1 -0.2 mol/L; the appropriate pH value of solution II was 2. No bands were amplified from mature leaves, tender leaves and old roots of Bt transgenic sugarcane, while root tips successfully amplified 545 bp target bands. The established method was used to identify and analyze 63 hybrid seedlings of YT 91 -976 × SBR216, results showed that a total of 17 samples exhibited 545 bp bands, accounting for approximately 27% of the total number of hybrid seedlings, indicating that these 17 seedlings were Bt transgenic sugarcane plants. [ Conclusion] The rapid detection method established in this study is conducive to identifying Bt transgenic sugarcane and improving breeding efficiency of insect-resistant sugarcane lines, which provides basis for screening of other transgenic materials.
文摘Seeds of japonica rice cv.Zhenuo 2 with twodifferent physiological states(dry seeds withwater content 13% and wet seeds soaked in thewater for 36 h)were irradiated by COlaser infour different power-densities and durations re-spectively.The treatment irradiated with 200GY ofCo γ-rays was considered as control.The flesh root tips were cut and fixed inCarnoy’s fluid for cytological examination.
文摘西南冬麦区气候冬干春旱频发、土壤速效磷缺乏,限制冬小麦氮素吸收。本研究探究秋闲期秸秆覆盖与施磷对小麦根系NO3-吸收动力势、氮素吸收利用、叶绿素含量和籽粒产量的影响,以期为小麦高产稳产及养分的高效利用提供理论依据。试验于2020—2022年在四川仁寿进行,采用二因素裂区设计,以秸秆覆盖(SM)和不覆盖(NSM)为主区;3个磷水平0(P0)、75(P75)和120(P120) kg hm-2为裂区。结果表明:秸秆覆盖与施磷显著提高地上部磷素积累量, SM较NSM的小麦根尖NO3-净吸收速率、籽粒氮积累量、氮素转运量、氮素同化量、氮肥偏生产力和籽粒产量分别增加28.2%、8.4%、9.0%、41.9%、23.3%和21.9%。与P0相比, P75和P120增加幅度分别达到35.1%~37.6%、12.6%~19.0%、7.1%~9.3%、35.7%~60.5%、17.6%~23.8%、17.2%~23.6%。与NSM相比, SM的小麦旗叶灌浆期叶绿素含量上升,进而提高籽粒产量。综上所述,秸秆覆盖与施磷可促进小麦根尖NO3-吸收,提高叶绿素含量,从而显著增加花后氮素的吸收及营养器官临时贮存氮素向籽粒的再分配,最终提高籽粒产量。考虑经济效益和产量回报,西南地区小麦高产高效栽培时,推荐采用秋闲期秸秆覆盖配施磷肥75 kg hm-2。
