In 2010, a foliar and stem-lesion disease that produced moderate to severe defoliation of watermelon was observed in the southern Great Plains. The disease was ultimately determined to be caused by Myrothecium roridum...In 2010, a foliar and stem-lesion disease that produced moderate to severe defoliation of watermelon was observed in the southern Great Plains. The disease was ultimately determined to be caused by Myrothecium roridum. The objective of this study was to compare the susceptibility of the vegetation and fruit of a broad range of commercially important cucurbits to three isolates obtained from these foliar lesions on watermelon. In greenhouse foliar inoculation experiments, cantaloupe, honeydew, cucumber, squash, and watermelon were susceptible to the fungus with cantaloupe and honeydew being the most susceptible and watermelon the most resistant. Furthermore, greenhouse inoculations supported earlier field observations as differential resistance was exhibited among the watermelon cultivars as well as the cucurbit types. All tested cucurbit fruit exhibited interior lesions when inoculated sub-epidermally with M. roridum isolates. However, natural infection of watermelon and pumpkin fruit has never been reported.展开更多
露湿漆斑菌产生的胆红素氧化酶粗酶液通过硫酸铵沉淀、DEAE-Sepharose Fast Flow和Sephadex G-100纯化,得到纯化152.54倍胆红素氧化酶,得率19.14%;用PAGE检测为单一条带,活性电泳证实该单一蛋白质组分为胆红素氧化酶,SDS-PAGE证明该酶...露湿漆斑菌产生的胆红素氧化酶粗酶液通过硫酸铵沉淀、DEAE-Sepharose Fast Flow和Sephadex G-100纯化,得到纯化152.54倍胆红素氧化酶,得率19.14%;用PAGE检测为单一条带,活性电泳证实该单一蛋白质组分为胆红素氧化酶,SDS-PAGE证明该酶的相对分子质量为64000。该酶最适反应温度为55℃,以胆红素为底物时最适pH值为7.5,而以ABTS为底物时最适pH值为4,该酶在pH 3-8之间都能够检测到酶活。以胆红素为底物,胆红素氧化酶的Km值和Vmax分别为552.06μmol/L和38.91μmol/(L.min);而以ABTSA为底物,胆红素氧化酶的Km值和Vmax分别为631.84μmol/L和12.79μmol/(L.min)。展开更多
A new leave disease of tomato(Lycopersicon esculentum Miller) was observed in polyethylene film-covered greenhouse in Beijing,China.This disease spreaded rapidly in the greenhouse and caused serious loss of the produc...A new leave disease of tomato(Lycopersicon esculentum Miller) was observed in polyethylene film-covered greenhouse in Beijing,China.This disease spreaded rapidly in the greenhouse and caused serious loss of the production of tomatoes.In the study,a fungal strain isolated from the lesion was confirmed to be pathogen for this new disease,and identified as Myrothecium roridum Tode ex Fr.based on the morphology,cultural characters on PDA plate and sequence analysis of ribosomal DNA-ITS.This is the first report of myrothecium leaf spot on tomato occurring in commercial greenhouse in China.展开更多
文摘In 2010, a foliar and stem-lesion disease that produced moderate to severe defoliation of watermelon was observed in the southern Great Plains. The disease was ultimately determined to be caused by Myrothecium roridum. The objective of this study was to compare the susceptibility of the vegetation and fruit of a broad range of commercially important cucurbits to three isolates obtained from these foliar lesions on watermelon. In greenhouse foliar inoculation experiments, cantaloupe, honeydew, cucumber, squash, and watermelon were susceptible to the fungus with cantaloupe and honeydew being the most susceptible and watermelon the most resistant. Furthermore, greenhouse inoculations supported earlier field observations as differential resistance was exhibited among the watermelon cultivars as well as the cucurbit types. All tested cucurbit fruit exhibited interior lesions when inoculated sub-epidermally with M. roridum isolates. However, natural infection of watermelon and pumpkin fruit has never been reported.
文摘露湿漆斑菌产生的胆红素氧化酶粗酶液通过硫酸铵沉淀、DEAE-Sepharose Fast Flow和Sephadex G-100纯化,得到纯化152.54倍胆红素氧化酶,得率19.14%;用PAGE检测为单一条带,活性电泳证实该单一蛋白质组分为胆红素氧化酶,SDS-PAGE证明该酶的相对分子质量为64000。该酶最适反应温度为55℃,以胆红素为底物时最适pH值为7.5,而以ABTS为底物时最适pH值为4,该酶在pH 3-8之间都能够检测到酶活。以胆红素为底物,胆红素氧化酶的Km值和Vmax分别为552.06μmol/L和38.91μmol/(L.min);而以ABTSA为底物,胆红素氧化酶的Km值和Vmax分别为631.84μmol/L和12.79μmol/(L.min)。
文摘A new leave disease of tomato(Lycopersicon esculentum Miller) was observed in polyethylene film-covered greenhouse in Beijing,China.This disease spreaded rapidly in the greenhouse and caused serious loss of the production of tomatoes.In the study,a fungal strain isolated from the lesion was confirmed to be pathogen for this new disease,and identified as Myrothecium roridum Tode ex Fr.based on the morphology,cultural characters on PDA plate and sequence analysis of ribosomal DNA-ITS.This is the first report of myrothecium leaf spot on tomato occurring in commercial greenhouse in China.