An innovative photoelectrode, TiO 2/Ti mesh electrode, was prepared by galvanostaticanodisation. The morphology and the crystalline texture of the TiO 2 film on mesh electrode were examined by scanning electronic mi...An innovative photoelectrode, TiO 2/Ti mesh electrode, was prepared by galvanostaticanodisation. The morphology and the crystalline texture of the TiO 2 film on mesh electrode were examined by scanning electronic microscopy and Raman spectroscopy respectively. The examination results indicated that the structure and properties of the film depended on anodisation rate, and the anatase was the dominant component under the controlled experimental conditions. Degradation of Rose Bengal(RB) in photocatalytic(PC) and photoelectrocatalytic(PEC) reaction was investigated, the results demonstrated that electric biasing could improve the efficiency of photocatalytic reaction. The measurement results of TOC in photoelectrocatalytic degradation showed that the mineralisation of RB was complete relatively. The comparison between the degradation efficiency of RB in PEC process and that in aqueous TiO 2 dispersion was conducted. The results showed that the apparent first order rate constant of RB degradation in PEC process was larger than that in aqueous dispersion with 0 1%—0 3% TiO 2 powder, but was smaller than that in aqueous dispersion with 1 0% TiO 2展开更多
The molecular thin films of Rose Bengal （RB） embedded in polymethyl methacrylate matrix are fabricated by using the spin-coating technique. The macroscopic current-voltage （I-V） characterization of the film shows ...The molecular thin films of Rose Bengal （RB） embedded in polymethyl methacrylate matrix are fabricated by using the spin-coating technique. The macroscopic current-voltage （I-V） characterization of the film shows that the RB molecule has two conductance switching states with a high ON/OFF ratio in ambient conditions. The infrared spectra indicate that intermolecular hydrogen bonds can form in the RB thin films after their hydrolysis in air. With the first-principles calculations, we demonstrate that the hydrogen bonds will be destroyed in concomitance with the conformational change when the RB molecule switches to its high-conductance state after applying a voltage.展开更多
The electric property and structure of some fluorescein derivatives and their rare earthcomplexes have been reported before.In order to investigate the effect of differentsubstituents on the electric property,the chan...The electric property and structure of some fluorescein derivatives and their rare earthcomplexes have been reported before.In order to investigate the effect of differentsubstituents on the electric property,the change of resistivity with temperature of eosin B(disodium salt of dibromodinitrofluorescein),rose bengal (disodium salt of tetrachlorotetraiodofluorescein),eosin 10B (dipotassium salt of dichlorotetrabromofluorescein) andtheir complexes with La(Ⅲ),Ce(Ⅲ),Gd(Ⅲ) and Y(Ⅲ) is studied in this paper.展开更多
Brucellosis is a zoonotic disease of economic importance. The clinical manifestations vary in humans;therefore a good diagnostic test is required to confirm the disease. The serum tube agglutination (SA) test, though ...Brucellosis is a zoonotic disease of economic importance. The clinical manifestations vary in humans;therefore a good diagnostic test is required to confirm the disease. The serum tube agglutination (SA) test, though still the most widely test used, can be problematic for the diagnosis of chronic infections. The other supplementary tests, such as the complement fixation (CF) test and ELISA, require special equipment, reagents and trained personnel. The Rose Bengal plate (RBP) test has shown potential as a good rapid diagnostic test. This is a report of serum samples from suspected cases of brucellosis that were tested using the RBP, SA and CF tests. The RBP test was shown to have a better relative sensitivity and as good specificity as the SA when compared with the CF test, and may be a useful initial diagnostic test for hospitals in remote rural areas if properly conducted with well stored antigen.展开更多
Rose Bengal (RB) is a potential photosuturing agent that may improve standard dermatologic surgical closure techniques. However, RB produces reactive oxygen species with photoactivation and its photomutagenic potentia...Rose Bengal (RB) is a potential photosuturing agent that may improve standard dermatologic surgical closure techniques. However, RB produces reactive oxygen species with photoactivation and its photomutagenic potential must be considered in clinical application. We investigated cytotoxicity, mutagenicity, and singlet oxygen (SO) production of RB on epithelial Chinese hamster ovary cell line. Cells were exposed to RB concentrations: 0.1%, 0.01%, 0.001%, 0.0001%, 0.00001%;irradiated for 400 s using a high-intensity visible wavelength lamp or maintained in the dark. Cell viability was assessed by XTT assay, mutagenicity by HPRT gene mutation assay, and SO production by Sensor Green reagent. RB > 0.001% was significantly cytotoxic. Viabilities were uninfluenced by ≤0.0001% RB controls, or 30-min incubation. 49% of irradiated cells died after 24-h in 0.0001% RB. At ≥0.001% RB, >90% of cells died. Irradiating 0.00001% - 0.001% RB increased SO;levels dropped significantly between 0.01% - 0.1%. Controls exhibited negligible SO production. HPRT suggested that RB was not mutagenic (0.0001%, 0.00001%);SO induction increased between 0.00001% - 0.001%, with reduced production at higher concentrations. Pilot studies suggested irradiated 0.0001% RB is mutagenic in vitro;current data suggest RB is not photomutagenic. The contribution of RB’s cytotoxicity on observed clinical improvement of scars and mutagenic potential remains unclear, necessitating further study.展开更多
UiO-66-NH2 is an efficient material for removing pollutants from wastewater due to its high specific surface area,high porosity and water stability.However,recycling them from wastewater is difficult.In this study,the...UiO-66-NH2 is an efficient material for removing pollutants from wastewater due to its high specific surface area,high porosity and water stability.However,recycling them from wastewater is difficult.In this study,the cellulose nanofibers mat deacetylated from cellulose acetate nanofibers were used to combine with UiO-66-NH_(2)by the method of in-situ growth to remove the toxic dye,rose bengal.Compared to previous work,the prepared composite could not only provide ease of separation of UiO-66-NH_(2)from the water after adsorption but also demonstrate better adsorption capacity(683 mg∙g‒1(T=25°C,pH=3))than that of the simple UiO-66-NH_(2)(309.6 mg∙g^(‒1)(T=25℃,pH=3)).Through the analysis of adsorption kinetics and isotherms,the adsorption for rose bengal is mainly suitable for the pseudo-second-order kinetic model and Freundlich model.Furthermore,the relevant research revealed that the main adsorption mechanism of the composite was electrostatic interaction,hydrogen bonding andπ–πinteraction.Overall,the approach depicts an efficient model for integrating metal-organic frameworks on cellulose nanofibers to improve metal-organic framework recovery performance with potentially broad applications.展开更多
Collagen is one of the most important biomaterials for tissue engineering approaches.Despite its excellent biocompatibility,it shows the non-negligible disadvantage of poor mechanical stability.Photochemical crosslink...Collagen is one of the most important biomaterials for tissue engineering approaches.Despite its excellent biocompatibility,it shows the non-negligible disadvantage of poor mechanical stability.Photochemical crosslinking with rose bengal and green light(RGX)is an appropriate method to improve this property.The development of collagen laminates is helpful for further adjustment of the mechanical properties as well as the controlled release of incorporated substances.In this study,we investigate the impact of crosslinking and layering of two different collagen scaffolds on the swelling behavior andmechanical behavior inmicro tensile tests to obtain information on its wearing comfort(stiffness,strength and ductility).The mechanical stability of the collagen material after degradation due to cell contact is examined using thickness measurements.There is no linear increase or decrease due to layering homologous laminates.Unexpectedly,a decrease in elongation at break,Young’s modulus and ultimate tensile strength are measured when the untreated monolayer is compared to the crosslinked one.Furthermore,we can detect a connection between stability and cell proliferation.The results show that with variation in number and type of layers,collagen scaffolds with tailored mechanical properties can be produced.Such a multi-layered structure enables the release of biomolecules into inner or outer layers for biomedical applications.展开更多
文摘An innovative photoelectrode, TiO 2/Ti mesh electrode, was prepared by galvanostaticanodisation. The morphology and the crystalline texture of the TiO 2 film on mesh electrode were examined by scanning electronic microscopy and Raman spectroscopy respectively. The examination results indicated that the structure and properties of the film depended on anodisation rate, and the anatase was the dominant component under the controlled experimental conditions. Degradation of Rose Bengal(RB) in photocatalytic(PC) and photoelectrocatalytic(PEC) reaction was investigated, the results demonstrated that electric biasing could improve the efficiency of photocatalytic reaction. The measurement results of TOC in photoelectrocatalytic degradation showed that the mineralisation of RB was complete relatively. The comparison between the degradation efficiency of RB in PEC process and that in aqueous TiO 2 dispersion was conducted. The results showed that the apparent first order rate constant of RB degradation in PEC process was larger than that in aqueous dispersion with 0 1%—0 3% TiO 2 powder, but was smaller than that in aqueous dispersion with 1 0% TiO 2
基金Project supported by the National Natural Science Foundation of China (Grant Nos 60771037 and 10774176)the National High Technology Research and Development Program of China (Grant No 2006CB921305)
文摘The molecular thin films of Rose Bengal （RB） embedded in polymethyl methacrylate matrix are fabricated by using the spin-coating technique. The macroscopic current-voltage （I-V） characterization of the film shows that the RB molecule has two conductance switching states with a high ON/OFF ratio in ambient conditions. The infrared spectra indicate that intermolecular hydrogen bonds can form in the RB thin films after their hydrolysis in air. With the first-principles calculations, we demonstrate that the hydrogen bonds will be destroyed in concomitance with the conformational change when the RB molecule switches to its high-conductance state after applying a voltage.
文摘The electric property and structure of some fluorescein derivatives and their rare earthcomplexes have been reported before.In order to investigate the effect of differentsubstituents on the electric property,the change of resistivity with temperature of eosin B(disodium salt of dibromodinitrofluorescein),rose bengal (disodium salt of tetrachlorotetraiodofluorescein),eosin 10B (dipotassium salt of dichlorotetrabromofluorescein) andtheir complexes with La(Ⅲ),Ce(Ⅲ),Gd(Ⅲ) and Y(Ⅲ) is studied in this paper.
文摘Brucellosis is a zoonotic disease of economic importance. The clinical manifestations vary in humans;therefore a good diagnostic test is required to confirm the disease. The serum tube agglutination (SA) test, though still the most widely test used, can be problematic for the diagnosis of chronic infections. The other supplementary tests, such as the complement fixation (CF) test and ELISA, require special equipment, reagents and trained personnel. The Rose Bengal plate (RBP) test has shown potential as a good rapid diagnostic test. This is a report of serum samples from suspected cases of brucellosis that were tested using the RBP, SA and CF tests. The RBP test was shown to have a better relative sensitivity and as good specificity as the SA when compared with the CF test, and may be a useful initial diagnostic test for hospitals in remote rural areas if properly conducted with well stored antigen.
文摘Rose Bengal (RB) is a potential photosuturing agent that may improve standard dermatologic surgical closure techniques. However, RB produces reactive oxygen species with photoactivation and its photomutagenic potential must be considered in clinical application. We investigated cytotoxicity, mutagenicity, and singlet oxygen (SO) production of RB on epithelial Chinese hamster ovary cell line. Cells were exposed to RB concentrations: 0.1%, 0.01%, 0.001%, 0.0001%, 0.00001%;irradiated for 400 s using a high-intensity visible wavelength lamp or maintained in the dark. Cell viability was assessed by XTT assay, mutagenicity by HPRT gene mutation assay, and SO production by Sensor Green reagent. RB > 0.001% was significantly cytotoxic. Viabilities were uninfluenced by ≤0.0001% RB controls, or 30-min incubation. 49% of irradiated cells died after 24-h in 0.0001% RB. At ≥0.001% RB, >90% of cells died. Irradiating 0.00001% - 0.001% RB increased SO;levels dropped significantly between 0.01% - 0.1%. Controls exhibited negligible SO production. HPRT suggested that RB was not mutagenic (0.0001%, 0.00001%);SO induction increased between 0.00001% - 0.001%, with reduced production at higher concentrations. Pilot studies suggested irradiated 0.0001% RB is mutagenic in vitro;current data suggest RB is not photomutagenic. The contribution of RB’s cytotoxicity on observed clinical improvement of scars and mutagenic potential remains unclear, necessitating further study.
基金support of the Tianjin Natural Science Foundation(Grant No.18JCQNJC71900).
文摘UiO-66-NH2 is an efficient material for removing pollutants from wastewater due to its high specific surface area,high porosity and water stability.However,recycling them from wastewater is difficult.In this study,the cellulose nanofibers mat deacetylated from cellulose acetate nanofibers were used to combine with UiO-66-NH_(2)by the method of in-situ growth to remove the toxic dye,rose bengal.Compared to previous work,the prepared composite could not only provide ease of separation of UiO-66-NH_(2)from the water after adsorption but also demonstrate better adsorption capacity(683 mg∙g‒1(T=25°C,pH=3))than that of the simple UiO-66-NH_(2)(309.6 mg∙g^(‒1)(T=25℃,pH=3)).Through the analysis of adsorption kinetics and isotherms,the adsorption for rose bengal is mainly suitable for the pseudo-second-order kinetic model and Freundlich model.Furthermore,the relevant research revealed that the main adsorption mechanism of the composite was electrostatic interaction,hydrogen bonding andπ–πinteraction.Overall,the approach depicts an efficient model for integrating metal-organic frameworks on cellulose nanofibers to improve metal-organic framework recovery performance with potentially broad applications.
基金supported by the Deutsche Forschungsgemeinschaft(Project number:400569699).
文摘Collagen is one of the most important biomaterials for tissue engineering approaches.Despite its excellent biocompatibility,it shows the non-negligible disadvantage of poor mechanical stability.Photochemical crosslinking with rose bengal and green light(RGX)is an appropriate method to improve this property.The development of collagen laminates is helpful for further adjustment of the mechanical properties as well as the controlled release of incorporated substances.In this study,we investigate the impact of crosslinking and layering of two different collagen scaffolds on the swelling behavior andmechanical behavior inmicro tensile tests to obtain information on its wearing comfort(stiffness,strength and ductility).The mechanical stability of the collagen material after degradation due to cell contact is examined using thickness measurements.There is no linear increase or decrease due to layering homologous laminates.Unexpectedly,a decrease in elongation at break,Young’s modulus and ultimate tensile strength are measured when the untreated monolayer is compared to the crosslinked one.Furthermore,we can detect a connection between stability and cell proliferation.The results show that with variation in number and type of layers,collagen scaffolds with tailored mechanical properties can be produced.Such a multi-layered structure enables the release of biomolecules into inner or outer layers for biomedical applications.
