Rose(Rosa hybrida)is widely used for cut flowers and as garden plants.Stable and efficient transformation system is required for functional genomics of rose.Here,we established an efficient transformation method for r...Rose(Rosa hybrida)is widely used for cut flowers and as garden plants.Stable and efficient transformation system is required for functional genomics of rose.Here,we established an efficient transformation method for rose using Agrobacterium tumefaciens-mediated transformation of embryogenic callus.Expanding rose leaves were used as explants to induce somatic embryos,which were subjected to transformation with A.tumefaciens strain GV3101 using Green Fluorescence Protein(GFP)as a marker gene.It took about 8 months to generate transgenic shoots from embryogenic callus.PCR,RT-PCR,Southern and Western blotting,as well as stereoscopic fluorescence microscopy analysis demonstrated that GFP transgenes integrated stably into the rose genome.According to our data,a transformation efficiency of up to 6%can be achieved by following this optimized protocol.展开更多
Effect of explant, site of leaflet, induction period in the dark and combinations of plant growth regulators on direct adventitious bud induction and plant regeneration of Rosa hybrida Samantha was investigated. The r...Effect of explant, site of leaflet, induction period in the dark and combinations of plant growth regulators on direct adventitious bud induction and plant regeneration of Rosa hybrida Samantha was investigated. The results showed that after an induction period of 8 d on MS medium with 1.5 mg L-1 TDZ and 0.05 mg L-1 NAA in the dark and a subculture on MS medium with 0.5 mg L-1 BA and 0.01 mg L-1 NAA under light, the best plant regeneration was obtained and the regeneration frequencies of leaflets and petioles were 51.8 and 10% respectively. There was no significant difference in regeneration ability between leaflets at different sites of the compound leaves, longer time of induction in the dark or high concentration of auxin would cause callus formation, which was disadvantageous for shoot regeneration, and the regeneration frequency was significantly reduced. This regeneration system could be applied for genetic transformation of this cultivar in the future.展开更多
[Objective] This study aimed to select suitable cultivars of cut roses with prominent comprehensive performance which is suitable for the commercial promotion in the tropical region of South Hainan Province,including ...[Objective] This study aimed to select suitable cultivars of cut roses with prominent comprehensive performance which is suitable for the commercial promotion in the tropical region of South Hainan Province,including Sanya and the low-altitude area.[Method] Introduction experiment and production test were designed to observe the agronomic and economic traits of cultivars in a rose resources nursery established in Sanya.[Result] Through observation in the resources garden,18 varieties were chosen to conduct the introduction experiment,of which 8 varieties were selected to accomplish the production test.The results of the production test showed that ＇Vendela＇,＇Golden Emblem＇,＇Samantha＇ and ＇Tineke＇ showed the best adaptability;the cut flower quality of ＇Carola＇,＇Black Magic＇ and ＇Golden Emblem＇ belonged to the first grade;the yield of ＇Movie Star＇,＇Golden Emblem＇ and ＇Black Magic＇ was more than one million per hm2;except ＇Golden Emblem＇,the vase life of the other seven cultivars was more than 10 days;＇Carola＇,＇My Choice＇ and ＇Perfume White＇ had better disease-and insect-resistance;＇Carola＇,＇Vendela＇,＇Rouge Meilland＇,＇Tineke＇,＇Golden Emblem＇,＇Samantha＇,＇Perfume White＇,＇Saiun＇,＇Asagumo＇ and ＇Double Delight＇ were more resistant to blackspot,while ＇Black Magic＇,＇Versilla＇,＇Alec＇s Red＇ and ＇Movie Star＇ were less resistant to blackspot.[Conclusion] Cultivars of cut roses were able to grow and develop normally in the tropical coastal area of Hainan Province,including South Hainan and Sanya,where it was appropriate for the production of cut roses.However,it is necessary to pay attention to the prevention and control of thrips and blackspot.＇Black Magic＇,＇Carola＇,＇Vendela＇,＇Rouge Meilland＇,＇Golden Emblem＇,＇Tineke＇,＇Movie Star＇ and ＇Samantha＇ showed best comprehensive performance,which were thus worthy of large-scale popularization in Hainan Province.展开更多
The purpose of this paper is to investigate the differential responses of flower opening to ethylene in two cut rose cultivars, ‘Samantha’, whose opening process is promoted, and ‘Kardinal’, whose opening process ...The purpose of this paper is to investigate the differential responses of flower opening to ethylene in two cut rose cultivars, ‘Samantha’, whose opening process is promoted, and ‘Kardinal’, whose opening process is inhibited by ethylene. Ethylene production and 1- aminocyclopropane-1-carboxylate (ACC) synthase and oxidase activities were determined first. After ethylene treatment, ethylene production, ACC synthase (ACS) and ACC oxidase (ACO) activities in petals increased and peaked at the earlier stage (stage 3) in ‘Samantha’, and they were much more dramatically enhanced and peaked at the later stage (stage 4) in ‘Kardinal’ than control during vasing. cDNA fragments of three Rh-ACSs and one Rh- ACO genes were cloned and designated as Rh-ACS1, Rh-ACS2, Rh-ACS3 and Rh-ACO1 respectively. Northern blotting analysis revealed that, among three genes of ACS, ethylene-in- duced expression patterns of Rh-ACS3 gene corresponded to ACS activity and ethylene production in both cultivars. A more dramatic accumulation of Rh-ACS3 mRNA was induced by ethylene in ‘Kardinal’ than that of ‘Samantha’. As an ethylene action inhibitor, STS at concentration of 0.2 mmol/L generally inhib-ited the expression of Rh-ACSs and Rh-ACO in both cultivars, although it induced the expression of Rh-ACS3 transiently in ‘Kardinal’. Our results suggests that ‘Kardinal’ is more sensitive to ethylene than ‘Samantha’; and the changes of Rh-ACS3 expression caused by ethylene might be related to the acceleration of flower opening in ‘Samantha’ and the inhibition in ‘Kardinal’. Additional results indicated that three Rh-ACSs genes were differentially associated with flower opening and senescence as well as wounding.展开更多
基金The authors thank Dr.Manzhu Bao(Huazhong Agricultural University,Wuhan,China),Dr.Hibrand-Saint Oyant L.(INRA,Agrocampus-Ouest,Universitéd’Angers,Beaucouzé,France)and Dr.Fabrice Foucher(INRA,78026 Versailles Cedex,France)for their excellent suggestions.We are also grateful to Dr.Wenxue Li and Dr.Hongqiu Wang(Chinese Academy of Agricultural Sci-ences,Beijing,China)for assistance with the experiments.This work was supported by grants from National Natural Science Foundation of China(Grant No.31522049)Construction of Beijing Science and Technology Innovation and Service Capacity in Top Subjects(Grant No.CEFF-PXM2019_014207_000032).
文摘Rose(Rosa hybrida)is widely used for cut flowers and as garden plants.Stable and efficient transformation system is required for functional genomics of rose.Here,we established an efficient transformation method for rose using Agrobacterium tumefaciens-mediated transformation of embryogenic callus.Expanding rose leaves were used as explants to induce somatic embryos,which were subjected to transformation with A.tumefaciens strain GV3101 using Green Fluorescence Protein(GFP)as a marker gene.It took about 8 months to generate transgenic shoots from embryogenic callus.PCR,RT-PCR,Southern and Western blotting,as well as stereoscopic fluorescence microscopy analysis demonstrated that GFP transgenes integrated stably into the rose genome.According to our data,a transformation efficiency of up to 6%can be achieved by following this optimized protocol.
基金the National Natural Science Foundation of China(30170666).
文摘Effect of explant, site of leaflet, induction period in the dark and combinations of plant growth regulators on direct adventitious bud induction and plant regeneration of Rosa hybrida Samantha was investigated. The results showed that after an induction period of 8 d on MS medium with 1.5 mg L-1 TDZ and 0.05 mg L-1 NAA in the dark and a subculture on MS medium with 0.5 mg L-1 BA and 0.01 mg L-1 NAA under light, the best plant regeneration was obtained and the regeneration frequencies of leaflets and petioles were 51.8 and 10% respectively. There was no significant difference in regeneration ability between leaflets at different sites of the compound leaves, longer time of induction in the dark or high concentration of auxin would cause callus formation, which was disadvantageous for shoot regeneration, and the regeneration frequency was significantly reduced. This regeneration system could be applied for genetic transformation of this cultivar in the future.
基金Supported by the National Agricultural Sci-Tech Achievements Transformation Projects(2010GB2E200369)the National Spark Program Project(2011GA800004)~~
文摘[Objective] This study aimed to select suitable cultivars of cut roses with prominent comprehensive performance which is suitable for the commercial promotion in the tropical region of South Hainan Province,including Sanya and the low-altitude area.[Method] Introduction experiment and production test were designed to observe the agronomic and economic traits of cultivars in a rose resources nursery established in Sanya.[Result] Through observation in the resources garden,18 varieties were chosen to conduct the introduction experiment,of which 8 varieties were selected to accomplish the production test.The results of the production test showed that ＇Vendela＇,＇Golden Emblem＇,＇Samantha＇ and ＇Tineke＇ showed the best adaptability;the cut flower quality of ＇Carola＇,＇Black Magic＇ and ＇Golden Emblem＇ belonged to the first grade;the yield of ＇Movie Star＇,＇Golden Emblem＇ and ＇Black Magic＇ was more than one million per hm2;except ＇Golden Emblem＇,the vase life of the other seven cultivars was more than 10 days;＇Carola＇,＇My Choice＇ and ＇Perfume White＇ had better disease-and insect-resistance;＇Carola＇,＇Vendela＇,＇Rouge Meilland＇,＇Tineke＇,＇Golden Emblem＇,＇Samantha＇,＇Perfume White＇,＇Saiun＇,＇Asagumo＇ and ＇Double Delight＇ were more resistant to blackspot,while ＇Black Magic＇,＇Versilla＇,＇Alec＇s Red＇ and ＇Movie Star＇ were less resistant to blackspot.[Conclusion] Cultivars of cut roses were able to grow and develop normally in the tropical coastal area of Hainan Province,including South Hainan and Sanya,where it was appropriate for the production of cut roses.However,it is necessary to pay attention to the prevention and control of thrips and blackspot.＇Black Magic＇,＇Carola＇,＇Vendela＇,＇Rouge Meilland＇,＇Golden Emblem＇,＇Tineke＇,＇Movie Star＇ and ＇Samantha＇ showed best comprehensive performance,which were thus worthy of large-scale popularization in Hainan Province.
基金This work was supported by the Nat ional Natural Science Foundation of China(Grant No.30471220).
文摘The purpose of this paper is to investigate the differential responses of flower opening to ethylene in two cut rose cultivars, ‘Samantha’, whose opening process is promoted, and ‘Kardinal’, whose opening process is inhibited by ethylene. Ethylene production and 1- aminocyclopropane-1-carboxylate (ACC) synthase and oxidase activities were determined first. After ethylene treatment, ethylene production, ACC synthase (ACS) and ACC oxidase (ACO) activities in petals increased and peaked at the earlier stage (stage 3) in ‘Samantha’, and they were much more dramatically enhanced and peaked at the later stage (stage 4) in ‘Kardinal’ than control during vasing. cDNA fragments of three Rh-ACSs and one Rh- ACO genes were cloned and designated as Rh-ACS1, Rh-ACS2, Rh-ACS3 and Rh-ACO1 respectively. Northern blotting analysis revealed that, among three genes of ACS, ethylene-in- duced expression patterns of Rh-ACS3 gene corresponded to ACS activity and ethylene production in both cultivars. A more dramatic accumulation of Rh-ACS3 mRNA was induced by ethylene in ‘Kardinal’ than that of ‘Samantha’. As an ethylene action inhibitor, STS at concentration of 0.2 mmol/L generally inhib-ited the expression of Rh-ACSs and Rh-ACO in both cultivars, although it induced the expression of Rh-ACS3 transiently in ‘Kardinal’. Our results suggests that ‘Kardinal’ is more sensitive to ethylene than ‘Samantha’; and the changes of Rh-ACS3 expression caused by ethylene might be related to the acceleration of flower opening in ‘Samantha’ and the inhibition in ‘Kardinal’. Additional results indicated that three Rh-ACSs genes were differentially associated with flower opening and senescence as well as wounding.
