为提高冰雹探测算法(Hail Detection Algorithm, HDA)产品的可用性,针对2015—2020年普洱地区监测到的22次冰雹个例,利用新一代雷达业务应用软件ROSE2.0对相关雷达基数据进行回放及产品分析,以命中率、虚警率、临界成功指数为指标对HDA...为提高冰雹探测算法(Hail Detection Algorithm, HDA)产品的可用性,针对2015—2020年普洱地区监测到的22次冰雹个例,利用新一代雷达业务应用软件ROSE2.0对相关雷达基数据进行回放及产品分析,以命中率、虚警率、临界成功指数为指标对HDA算法在普洱地区的识别效果进行评估并给出本地化参数配置方案。结果表明:HDA算法在普洱地区命中率接近100%,但虚警现象非常普遍,使用强冰雹概率(Probability of Severe Hail, POSH)的预警效果优于任意大小冰雹概率(Probability of Hail, POH),且冰雹尺寸越大POSH虚警的概率越低。进一步使用模拟测评法对POSH算法的适配参数进行分析,发现正确输入降雹日当天的0℃层和-20℃层高度能有效减少POSH的虚警率及提高临界成功指数;同时使算法预测的最大冰雹直径普遍偏大的情况得到控制,其中,中小冰雹直径偏离百分比减小76.07%,改善效果显著高于大冰雹。此外,增大反射率因子及POSH阈值能有效控制虚警,但也导致漏报次数快速增加,当阈值太大时命中率明显降低,为了保证较高的命中率和临界成功指数,选择Z=50 dBz或POSH=70%为阈值能明显改善HDA算法的识别效果。展开更多
In order to reduce the waste of resources and environmental pollution caused by excessive application of chemical fertilizers, improve the utilization rate of fertilizers, and promote the large-scale and high-quality ...In order to reduce the waste of resources and environmental pollution caused by excessive application of chemical fertilizers, improve the utilization rate of fertilizers, and promote the large-scale and high-quality development of the Chinese rose industry. In this experiment, corn stover biochar, phosphoric acid modified biochar and organic fertilizer were used as test materials, and the effects of mixed application of modified biochar and organic fertilizer on the growth and development of Chinese rose as well as soil physicochemical properties were investigated by using the method of pot planting test. The results showed that modified biochar with organic fertilizer had the most significant effect on the enhancement of soil pH, organic matter content and soil carbon-to-nitrogen ratio. After 120 d of planting, modified biochar with organic fertilizer had the most significant effect on the enhancement of plant height and crown width of Chinese rose;both organic fertilizer and modified biochar with organic fertilizer significantly increased the chlorophyll content of Chinese rose. The number of flowers and the number of branches were the highest in the modified biochar with organic fertilizer treatment. In conclusion, the application of modified biochar with organic fertilizer can better improve the soil pH, and increase the soil organic matter content and carbon-to-nitrogen ratio to change the biological traits of Chinese rose. The results of this study provide a theoretical basis for the reduction of chemical fertilizers and the resource utilization of agricultural wastes and guarantee the sustainable development of the cut flower industry.展开更多
The novel A Rose for Emily is one of the early works of William Faulkner,a famous American southern writer who has won The Nobel Prize in Literature in 1950.Spatial narrative theory analyzes and summarizes the spatial...The novel A Rose for Emily is one of the early works of William Faulkner,a famous American southern writer who has won The Nobel Prize in Literature in 1950.Spatial narrative theory analyzes and summarizes the spatial characteristics of modern and postmodern novels,showing the narrative characteristics of the works that the time is disrupted and suspended,and the space is extended indefinitely.This paper intends to interpret A Rose for Emily from the perspective of spatial narrative theory,and analyze the geological space,social space and text space in the novel,so as to reveal the important role of spatial structure in creating atmosphere and promoting narrative process,in order to better grasp the spatial metaphors in Faulkner’s novels.展开更多
Rose(Rosa hybrida)is widely used for cut flowers and as garden plants.Stable and efficient transformation system is required for functional genomics of rose.Here,we established an efficient transformation method for r...Rose(Rosa hybrida)is widely used for cut flowers and as garden plants.Stable and efficient transformation system is required for functional genomics of rose.Here,we established an efficient transformation method for rose using Agrobacterium tumefaciens-mediated transformation of embryogenic callus.Expanding rose leaves were used as explants to induce somatic embryos,which were subjected to transformation with A.tumefaciens strain GV3101 using Green Fluorescence Protein(GFP)as a marker gene.It took about 8 months to generate transgenic shoots from embryogenic callus.PCR,RT-PCR,Southern and Western blotting,as well as stereoscopic fluorescence microscopy analysis demonstrated that GFP transgenes integrated stably into the rose genome.According to our data,a transformation efficiency of up to 6%can be achieved by following this optimized protocol.展开更多
Phytohormones are key factors in plant responsiveness to abiotic and biotic stresses,and maintaining hormone homeostasis is critically important during stress responses.Cut rose(Rosa hybrida)flowers experience dehydra...Phytohormones are key factors in plant responsiveness to abiotic and biotic stresses,and maintaining hormone homeostasis is critically important during stress responses.Cut rose(Rosa hybrida)flowers experience dehydration stress during postharvest handling,and jasmonic acid(JA)levels change as a result of this stress.However,how JA is involved in dehydration tolerance remains unclear.We investigated the functions of the JA-and dehydration-induced RhHB1 gene,which encodes a homeodomain-leucine zipper Iγ-clade transcription factor,in rose flowers.Silencing RhHB1 decreased petal dehydration tolerance and resulted in a persistent increase in JA-Ile content and reduced dehydration tolerance.An elevated JA-Ile level had a detrimental effect on rose petal dehydration tolerance.RhHB1 was shown to lower the transient induction of JA-Ile accumulation in response to dehydration.In addition to transcriptomic data,we obtained evidence that RhHB1 suppresses the expression of the lipoxygenase 4(RhLOX4)gene by directly binding to its promoter both in vivo and in vitro.We propose that increased JA-Ile levels weaken the capacity for osmotic adjustment in petal cells,resulting in reduced dehydration tolerance.In conclusion,a JA feedback loop mediated by an RhHB1/RhLOX4 regulatory module provides dehydration tolerance by fine-tuning bioactive JA levels in dehydrated flowers.展开更多
The consumption of cut roses(Rosa hybrida)has always ranked first in the world.However,it is vulnerable to rapid petal and leaf wilting due to leaf stomatal water loss,which seriously affects its ornamental quality an...The consumption of cut roses(Rosa hybrida)has always ranked first in the world.However,it is vulnerable to rapid petal and leaf wilting due to leaf stomatal water loss,which seriously affects its ornamental quality and economic value.Stomatal movement,a key in plant physiological processes,is influenced by potassium and nitrate.Advancing comprehension of its physiological and molecular mechanism holds promise for preserving the freshness of cut roses.This study observed the impacts of different concentrations of KNO_(3) vase treatments on stomatal opening and water loss in cut rose‘Carola’leaves,as well as their transcriptional responses to KNO_(3).Water loss rates were influenced by KNO_(3) concentrations,with the 25 and 75 mmol/L treatments exhibiting the highest water loss rates.The stomatal aperture reached its widest value when treated with 75 mmol/L KNO_(3).Transcriptional sequencing analysis was performed to identify differentially expressed genes(DEGs)of which 5456 were up-regulated,and 6607 were down-regulated associated with photosynthesis,starch and sucrose metabolism,metabolic pathways,plant-pathogen interaction,plant hormone signal transduction,and related pathways.246 DEGs were selected related to response to KNO_(3) treatment,of which gene ontology(GO)enrichment were nitrate and terpenoid metabolism,ion transport,and response to stimuli.Further heatmap analysis revealed that several genes related to nitrate transport a metabolism,K+transport,vacuoles,and aquaporin were in close association with the response to KNO_(3) treatment.Weighted gene co-expression network analysis(WGCNA)revealed that hub genes,including LAX2,TSJT1,and SCPL34 were identified in turquoise,black,and darkgreen module.Transcription factors such as NAC021,CDF3,ERF053,ETR2,and ARF6 exhibited regulatory roles in the response to KNO_(3) treatment under light conditions.These findings provide valuable insights into the physiological and molecular mechanisms underlying the response of cut rose leaves to KNO_(3) treatment.展开更多
本文基于贝尔曼的“否定分析”理论,对福克纳短篇小说A Rose for Emily杨岂深汉译本进行分析,试图验证分析其中的文本变形倾向,从而对小说翻译的实践起到一定的促进作用。经过研究发现:杨岂深先生版本的译文中理性化、高贵化的倾向较多...本文基于贝尔曼的“否定分析”理论,对福克纳短篇小说A Rose for Emily杨岂深汉译本进行分析,试图验证分析其中的文本变形倾向,从而对小说翻译的实践起到一定的促进作用。经过研究发现:杨岂深先生版本的译文中理性化、高贵化的倾向较多,兼有对原文节奏的破坏以及原文意指体系的破坏等变形倾向,因而导致原文口语化的叙事风格总体偏向较为正式且古雅的风格,尤其是译文中大量四字成语以及书面语的使用。同时,从文中部分词汇的翻译也可看出译者在进行翻译时采取了贴近译入语语言文化习惯的翻译方式,验证了翻译过程中文本变形倾向的存在。但从某种程度上来看,这种翻译的方式一定程度上与原文的语言风格有所差异,原文中的异质性则被过滤了。展开更多
William Faulkner(1897-1962)is usually regarded as one of the most important American novelist s of the 20th century,who was awarded the Nobel Prize in Literature in 1949.“A Rose for Emily”is one of Faulkner’s famou...William Faulkner(1897-1962)is usually regarded as one of the most important American novelist s of the 20th century,who was awarded the Nobel Prize in Literature in 1949.“A Rose for Emily”is one of Faulkner’s famous short stories which applies the conventions of Gothic fiction,and it has drawn the the attention of a large number of scholars and inspired their enthusiasm of interpretation owing to its use of many experimental techniques.This article attempts to analyze the narrative techniques of“A Rose for Emily”in terms of tense,mood and voice,three concepts introduced by Gerald Genette,a distinguished French critic of structuralist narratology in his Narrative Discourse(1980).展开更多
In 1569,Giorgio Vasari(1511-1574)completed painting an altarpiece of the Madonna of the Rosary and a Tondo of angels dispersing roses.The commission was for the private chapel of the Capponi family in the church of Sa...In 1569,Giorgio Vasari(1511-1574)completed painting an altarpiece of the Madonna of the Rosary and a Tondo of angels dispersing roses.The commission was for the private chapel of the Capponi family in the church of Santa Maria Novella in Florence.In his Ricordanze(Book of Records),Vasari explained the commission as well as documenting the assistance of his favorite Florentine pupil,Jacopo Zucchi(1541-1590),in the completion of the commission.This essay focusses on Vasari’s design,location,and meaning of the Tondo and its emblematic symbolism of love through the rose motif.展开更多
使用医疗信息系统的数据进行睡眠呼吸暂停低通气综合征(OSAHS)预测和分析过程中,存在不平衡数据问题。为此,在现有临床研究的基础上,提出了一种基于ROSE(Random Over Sampling Examples)和C5.0算法的初筛模型。利用收集到的人体测量学...使用医疗信息系统的数据进行睡眠呼吸暂停低通气综合征(OSAHS)预测和分析过程中,存在不平衡数据问题。为此,在现有临床研究的基础上,提出了一种基于ROSE(Random Over Sampling Examples)和C5.0算法的初筛模型。利用收集到的人体测量学指标数据,通过数据预处理,删除异常值并填补缺失值。然后采用ROSE算法对数据进行平衡,利用C5.0分类器对平衡后的数据构建筛查模型,通过十则交叉验证的方法检验模型的筛查效果。实验结果表明,使用该模型进行打鼾患者的OSAHS筛查,可以有效地提高筛查效率。展开更多
文摘为提高冰雹探测算法(Hail Detection Algorithm, HDA)产品的可用性,针对2015—2020年普洱地区监测到的22次冰雹个例,利用新一代雷达业务应用软件ROSE2.0对相关雷达基数据进行回放及产品分析,以命中率、虚警率、临界成功指数为指标对HDA算法在普洱地区的识别效果进行评估并给出本地化参数配置方案。结果表明:HDA算法在普洱地区命中率接近100%,但虚警现象非常普遍,使用强冰雹概率(Probability of Severe Hail, POSH)的预警效果优于任意大小冰雹概率(Probability of Hail, POH),且冰雹尺寸越大POSH虚警的概率越低。进一步使用模拟测评法对POSH算法的适配参数进行分析,发现正确输入降雹日当天的0℃层和-20℃层高度能有效减少POSH的虚警率及提高临界成功指数;同时使算法预测的最大冰雹直径普遍偏大的情况得到控制,其中,中小冰雹直径偏离百分比减小76.07%,改善效果显著高于大冰雹。此外,增大反射率因子及POSH阈值能有效控制虚警,但也导致漏报次数快速增加,当阈值太大时命中率明显降低,为了保证较高的命中率和临界成功指数,选择Z=50 dBz或POSH=70%为阈值能明显改善HDA算法的识别效果。
文摘In order to reduce the waste of resources and environmental pollution caused by excessive application of chemical fertilizers, improve the utilization rate of fertilizers, and promote the large-scale and high-quality development of the Chinese rose industry. In this experiment, corn stover biochar, phosphoric acid modified biochar and organic fertilizer were used as test materials, and the effects of mixed application of modified biochar and organic fertilizer on the growth and development of Chinese rose as well as soil physicochemical properties were investigated by using the method of pot planting test. The results showed that modified biochar with organic fertilizer had the most significant effect on the enhancement of soil pH, organic matter content and soil carbon-to-nitrogen ratio. After 120 d of planting, modified biochar with organic fertilizer had the most significant effect on the enhancement of plant height and crown width of Chinese rose;both organic fertilizer and modified biochar with organic fertilizer significantly increased the chlorophyll content of Chinese rose. The number of flowers and the number of branches were the highest in the modified biochar with organic fertilizer treatment. In conclusion, the application of modified biochar with organic fertilizer can better improve the soil pH, and increase the soil organic matter content and carbon-to-nitrogen ratio to change the biological traits of Chinese rose. The results of this study provide a theoretical basis for the reduction of chemical fertilizers and the resource utilization of agricultural wastes and guarantee the sustainable development of the cut flower industry.
文摘The novel A Rose for Emily is one of the early works of William Faulkner,a famous American southern writer who has won The Nobel Prize in Literature in 1950.Spatial narrative theory analyzes and summarizes the spatial characteristics of modern and postmodern novels,showing the narrative characteristics of the works that the time is disrupted and suspended,and the space is extended indefinitely.This paper intends to interpret A Rose for Emily from the perspective of spatial narrative theory,and analyze the geological space,social space and text space in the novel,so as to reveal the important role of spatial structure in creating atmosphere and promoting narrative process,in order to better grasp the spatial metaphors in Faulkner’s novels.
基金The authors thank Dr.Manzhu Bao(Huazhong Agricultural University,Wuhan,China),Dr.Hibrand-Saint Oyant L.(INRA,Agrocampus-Ouest,Universitéd’Angers,Beaucouzé,France)and Dr.Fabrice Foucher(INRA,78026 Versailles Cedex,France)for their excellent suggestions.We are also grateful to Dr.Wenxue Li and Dr.Hongqiu Wang(Chinese Academy of Agricultural Sci-ences,Beijing,China)for assistance with the experiments.This work was supported by grants from National Natural Science Foundation of China(Grant No.31522049)Construction of Beijing Science and Technology Innovation and Service Capacity in Top Subjects(Grant No.CEFF-PXM2019_014207_000032).
文摘Rose(Rosa hybrida)is widely used for cut flowers and as garden plants.Stable and efficient transformation system is required for functional genomics of rose.Here,we established an efficient transformation method for rose using Agrobacterium tumefaciens-mediated transformation of embryogenic callus.Expanding rose leaves were used as explants to induce somatic embryos,which were subjected to transformation with A.tumefaciens strain GV3101 using Green Fluorescence Protein(GFP)as a marker gene.It took about 8 months to generate transgenic shoots from embryogenic callus.PCR,RT-PCR,Southern and Western blotting,as well as stereoscopic fluorescence microscopy analysis demonstrated that GFP transgenes integrated stably into the rose genome.According to our data,a transformation efficiency of up to 6%can be achieved by following this optimized protocol.
基金funded by the National Key R&D Program of China(2018YFD1000407)the Construction of Beijing Science and Technology Innovation and Service Capacity in Top Subjects(CEFFPXM2019_014207_000032).
文摘Phytohormones are key factors in plant responsiveness to abiotic and biotic stresses,and maintaining hormone homeostasis is critically important during stress responses.Cut rose(Rosa hybrida)flowers experience dehydration stress during postharvest handling,and jasmonic acid(JA)levels change as a result of this stress.However,how JA is involved in dehydration tolerance remains unclear.We investigated the functions of the JA-and dehydration-induced RhHB1 gene,which encodes a homeodomain-leucine zipper Iγ-clade transcription factor,in rose flowers.Silencing RhHB1 decreased petal dehydration tolerance and resulted in a persistent increase in JA-Ile content and reduced dehydration tolerance.An elevated JA-Ile level had a detrimental effect on rose petal dehydration tolerance.RhHB1 was shown to lower the transient induction of JA-Ile accumulation in response to dehydration.In addition to transcriptomic data,we obtained evidence that RhHB1 suppresses the expression of the lipoxygenase 4(RhLOX4)gene by directly binding to its promoter both in vivo and in vitro.We propose that increased JA-Ile levels weaken the capacity for osmotic adjustment in petal cells,resulting in reduced dehydration tolerance.In conclusion,a JA feedback loop mediated by an RhHB1/RhLOX4 regulatory module provides dehydration tolerance by fine-tuning bioactive JA levels in dehydrated flowers.
基金supported by the National Natural Science Foundation of China (Nos.32002069 and 31972439)the Basic and Applied Research Project of Guangdong Province (2020A1515110961).
文摘The consumption of cut roses(Rosa hybrida)has always ranked first in the world.However,it is vulnerable to rapid petal and leaf wilting due to leaf stomatal water loss,which seriously affects its ornamental quality and economic value.Stomatal movement,a key in plant physiological processes,is influenced by potassium and nitrate.Advancing comprehension of its physiological and molecular mechanism holds promise for preserving the freshness of cut roses.This study observed the impacts of different concentrations of KNO_(3) vase treatments on stomatal opening and water loss in cut rose‘Carola’leaves,as well as their transcriptional responses to KNO_(3).Water loss rates were influenced by KNO_(3) concentrations,with the 25 and 75 mmol/L treatments exhibiting the highest water loss rates.The stomatal aperture reached its widest value when treated with 75 mmol/L KNO_(3).Transcriptional sequencing analysis was performed to identify differentially expressed genes(DEGs)of which 5456 were up-regulated,and 6607 were down-regulated associated with photosynthesis,starch and sucrose metabolism,metabolic pathways,plant-pathogen interaction,plant hormone signal transduction,and related pathways.246 DEGs were selected related to response to KNO_(3) treatment,of which gene ontology(GO)enrichment were nitrate and terpenoid metabolism,ion transport,and response to stimuli.Further heatmap analysis revealed that several genes related to nitrate transport a metabolism,K+transport,vacuoles,and aquaporin were in close association with the response to KNO_(3) treatment.Weighted gene co-expression network analysis(WGCNA)revealed that hub genes,including LAX2,TSJT1,and SCPL34 were identified in turquoise,black,and darkgreen module.Transcription factors such as NAC021,CDF3,ERF053,ETR2,and ARF6 exhibited regulatory roles in the response to KNO_(3) treatment under light conditions.These findings provide valuable insights into the physiological and molecular mechanisms underlying the response of cut rose leaves to KNO_(3) treatment.
文摘本文基于贝尔曼的“否定分析”理论,对福克纳短篇小说A Rose for Emily杨岂深汉译本进行分析,试图验证分析其中的文本变形倾向,从而对小说翻译的实践起到一定的促进作用。经过研究发现:杨岂深先生版本的译文中理性化、高贵化的倾向较多,兼有对原文节奏的破坏以及原文意指体系的破坏等变形倾向,因而导致原文口语化的叙事风格总体偏向较为正式且古雅的风格,尤其是译文中大量四字成语以及书面语的使用。同时,从文中部分词汇的翻译也可看出译者在进行翻译时采取了贴近译入语语言文化习惯的翻译方式,验证了翻译过程中文本变形倾向的存在。但从某种程度上来看,这种翻译的方式一定程度上与原文的语言风格有所差异,原文中的异质性则被过滤了。
文摘William Faulkner(1897-1962)is usually regarded as one of the most important American novelist s of the 20th century,who was awarded the Nobel Prize in Literature in 1949.“A Rose for Emily”is one of Faulkner’s famous short stories which applies the conventions of Gothic fiction,and it has drawn the the attention of a large number of scholars and inspired their enthusiasm of interpretation owing to its use of many experimental techniques.This article attempts to analyze the narrative techniques of“A Rose for Emily”in terms of tense,mood and voice,three concepts introduced by Gerald Genette,a distinguished French critic of structuralist narratology in his Narrative Discourse(1980).
文摘In 1569,Giorgio Vasari(1511-1574)completed painting an altarpiece of the Madonna of the Rosary and a Tondo of angels dispersing roses.The commission was for the private chapel of the Capponi family in the church of Santa Maria Novella in Florence.In his Ricordanze(Book of Records),Vasari explained the commission as well as documenting the assistance of his favorite Florentine pupil,Jacopo Zucchi(1541-1590),in the completion of the commission.This essay focusses on Vasari’s design,location,and meaning of the Tondo and its emblematic symbolism of love through the rose motif.
文摘使用医疗信息系统的数据进行睡眠呼吸暂停低通气综合征(OSAHS)预测和分析过程中,存在不平衡数据问题。为此,在现有临床研究的基础上,提出了一种基于ROSE(Random Over Sampling Examples)和C5.0算法的初筛模型。利用收集到的人体测量学指标数据,通过数据预处理,删除异常值并填补缺失值。然后采用ROSE算法对数据进行平衡,利用C5.0分类器对平衡后的数据构建筛查模型,通过十则交叉验证的方法检验模型的筛查效果。实验结果表明,使用该模型进行打鼾患者的OSAHS筛查,可以有效地提高筛查效率。
