[ Objective] To study the effects of combined application of different elements on alfalfa hay yield. [ Medthod] Potassium sulfate, ammonium chloride, superphosphate and magnesium oxide were applied to alfalfa field i...[ Objective] To study the effects of combined application of different elements on alfalfa hay yield. [ Medthod] Potassium sulfate, ammonium chloride, superphosphate and magnesium oxide were applied to alfalfa field in the experiment, and quaternary quadratic regression orthogonal combination was used to examine the effects of the experiment factors on hay yield of alfalfa. [ Result] The order of targets based on the effects on hay yield is as follows: potassium sulfate 〉 ammonium chloride 〉 magnesium oxide 〉 superphosphate. Among them, the potassium sulfate had a significant impact on alfalfa hay yield. The interaction between potassium sulfate and magnesium oxide also had a significant impact on alfalfa hay yield. [ Conclusion] The optimal cultivation measures for alfalfa hay yield of more than 19 224.6 kg/hm^2 are as follows: potassium sulfate, 163.253 - 181.679 kg/hm^2 ; ammonium chloride, 155.877 - 174.258 kg/hm^2 ; superphosphate, 566.177 - 632.924 kg/hm^2 ; and magnesium oxide, 30.060 - 33.628 kg/hm^2.展开更多
Effects of Mg^2+, NTPs and Taq DNA polymerase in pear SSR-PCR system were analyzed by quadratic regressive orthogonal rotational combinational design. Results showed: absolute IOD (Integrated OD of each band) valu...Effects of Mg^2+, NTPs and Taq DNA polymerase in pear SSR-PCR system were analyzed by quadratic regressive orthogonal rotational combinational design. Results showed: absolute IOD (Integrated OD of each band) value of target band reduced with concentration rising of Mg^2+ and Taq DNA polymerase, but heightened with concentration rising of dNTPs. The decay rate of absolute IOD value increased progressively with rising Mg^2+ concentration, decreased gradually with rising Taq DNA polymerase concentration; the rising speed would be slower than the dNTPs increase. Absolute IOD value would reduce with concentration rising of dNTPs at a low level of Mg^2+ concentration. Conversely it would rise with the increase of dNTPs while high Mg^2+ concentration. Absolute IOD value would generally rise with concentration rising of Taq DNA polymerase while low Mg^2+ concentration. On the contrary it would reduce with concentration rising of Taq DNA polymerase while high Mg^2+ concentration.展开更多
基金funded by the National Key Technology R&D Program (2006BAD04A10)the Major Scientific and Technological Innovation Projects of Hebei Province (07227146Z)the Earmarked Fund for Modern Agro-industry (Dairy Industry) Technology Research System (nycytx-10)
文摘[ Objective] To study the effects of combined application of different elements on alfalfa hay yield. [ Medthod] Potassium sulfate, ammonium chloride, superphosphate and magnesium oxide were applied to alfalfa field in the experiment, and quaternary quadratic regression orthogonal combination was used to examine the effects of the experiment factors on hay yield of alfalfa. [ Result] The order of targets based on the effects on hay yield is as follows: potassium sulfate 〉 ammonium chloride 〉 magnesium oxide 〉 superphosphate. Among them, the potassium sulfate had a significant impact on alfalfa hay yield. The interaction between potassium sulfate and magnesium oxide also had a significant impact on alfalfa hay yield. [ Conclusion] The optimal cultivation measures for alfalfa hay yield of more than 19 224.6 kg/hm^2 are as follows: potassium sulfate, 163.253 - 181.679 kg/hm^2 ; ammonium chloride, 155.877 - 174.258 kg/hm^2 ; superphosphate, 566.177 - 632.924 kg/hm^2 ; and magnesium oxide, 30.060 - 33.628 kg/hm^2.
文摘Effects of Mg^2+, NTPs and Taq DNA polymerase in pear SSR-PCR system were analyzed by quadratic regressive orthogonal rotational combinational design. Results showed: absolute IOD (Integrated OD of each band) value of target band reduced with concentration rising of Mg^2+ and Taq DNA polymerase, but heightened with concentration rising of dNTPs. The decay rate of absolute IOD value increased progressively with rising Mg^2+ concentration, decreased gradually with rising Taq DNA polymerase concentration; the rising speed would be slower than the dNTPs increase. Absolute IOD value would reduce with concentration rising of dNTPs at a low level of Mg^2+ concentration. Conversely it would rise with the increase of dNTPs while high Mg^2+ concentration. Absolute IOD value would generally rise with concentration rising of Taq DNA polymerase while low Mg^2+ concentration. On the contrary it would reduce with concentration rising of Taq DNA polymerase while high Mg^2+ concentration.