湖北贝母为传统中药,然而《Flora of China》将其基原植物湖北贝母Fritillaria hupehensis归并于天目贝母F.monantha项下。该实验采用分子系统学方法,以川百合Lilium davidii为外类群,用核基因ITS序列和叶绿体基因rpl16序列、matK序列等...湖北贝母为传统中药,然而《Flora of China》将其基原植物湖北贝母Fritillaria hupehensis归并于天目贝母F.monantha项下。该实验采用分子系统学方法,以川百合Lilium davidii为外类群,用核基因ITS序列和叶绿体基因rpl16序列、matK序列等3个片段对湖北贝母及其近缘类群天目贝母F.monantha、安徽贝母F.anhuiensis等进行联合建树分析,对湖北贝母植物的系统位置进行了探讨,为湖北贝母药材的安全使用提供分子证据。结果显示,分子系统树上,3种贝母各自的居群聚为一支,之后天目贝母与安徽贝母聚为一支,最后与湖北贝母聚为一支。表明湖北贝母与天目贝母的亲缘关系可能要远于安徽贝母与天目贝母之间的关系,因此不适宜将湖北贝母归并于天目贝母。展开更多
Genera Lamiophlomis and Paraphlomis were originally separated from genus Phlomis s.1. on the basis of particular morphological characteristics. However, their relationship was highly contentious, as evidenced by the l...Genera Lamiophlomis and Paraphlomis were originally separated from genus Phlomis s.1. on the basis of particular morphological characteristics. However, their relationship was highly contentious, as evidenced by the literature. In the present paper, the systematic positions of Lamiophlomis, Paraphlomis, and their related genera were assessed based on nuclear internal transcribed spacer （ITS） and chloroplast rpl16 and trnL-F sequence data using maximum parsimony （MP） and Bayesian methods. and outgroup were sampled. Analyses of both separate In total, 24 species representing six genera of the ingroup and combined sequence data were conducted to resolve the systematic relationships of these genera. The results reveal that Lamiophlomis is nested within Phlomis sect. Phlomoides and its generic status is not supported. With the inclusion ofLamiophlomis rotata in sect. Phlomoides, sections Phlomis and Phlomoides of Phlomis were resolved as monophyletic. Paraphlomis was supported as an independent genus. However, the resolution of its monophyly conflicted between MP and Bayesian analyses, suggesting the need for expended sampling and further evidence.展开更多
<正> Eighteen representative species were selected from all the nine sections of Chinese Allium on the basis of the classification of morphology and cytotaxonomy. The trnK and rpL16 gene fragments of chloroplast...<正> Eighteen representative species were selected from all the nine sections of Chinese Allium on the basis of the classification of morphology and cytotaxonomy. The trnK and rpL16 gene fragments of chloroplast DNA were amplified from 18 species by PCR method. The two cpDNA fragments were digested by 26 restriction enzymes, and 303 polymorphic restriction sites were found, of which 163 were informative. The restriction site data were analyzed with PAUP (version 3.1.1) and MEGA (version 1.01) as well as PHYLIP. As a result, the genus Allium could be classified into six subgenera. The recognition of Sect. Anguinum in the Flora of China is reasonable, Sect. Rhizirideum, Sect. Haplostemon and Sect. Cepa are not monophyletic. The infrageneric system of this genus was also discussed.展开更多
基金grants from the Ministry of Science and Technology(2005DKA21006)to De-Zhu LIthe Yunnan Pro-vincial Natural Science Foundation(2005C00-50M)to Lian-Ming GAO.
文摘大百合是百合科大百合属一种重要的野生植物资源,本研究采用基因测序法,利用叶绿体DNA rpl16序列对10个大百合居群进行了遗传多样性和遗传结构分析,旨在为其资源保护和开发利用提供理论基础。结果显示:rpl16序列矩阵长度为699 bp,共检测到14个变异位点和12个单倍型,单倍型多态性(Hd)为0.604;总的遗传多样性(HT)为0.660。该居群间遗传变异(71.53%)大于居群内遗传变异(28.47%),说明居群间变异是其居群的主要变异来源;居群间遗传分化很高(Gst=0.677,Nst=0.614,Fst=0.71531),基因流较低(Nm=0.0995)。失配分析结果表明种群在进化过程中曾经历过快速扩张,中性检验支持上述结果:Tajima′s D=-1.74368(P>0.05);Fu and Li′s D=-2.69366(P<0.05);Fu and Li′s F=-2.79896(P<0.05)。研究结果表明:大百合居群遗传多样性较高,居群间存在较高的遗传分化,主要与其生活史特征相关。基于得到的居群遗传信息,建议采取就地保护为主的保护策略。
文摘湖北贝母为传统中药,然而《Flora of China》将其基原植物湖北贝母Fritillaria hupehensis归并于天目贝母F.monantha项下。该实验采用分子系统学方法,以川百合Lilium davidii为外类群,用核基因ITS序列和叶绿体基因rpl16序列、matK序列等3个片段对湖北贝母及其近缘类群天目贝母F.monantha、安徽贝母F.anhuiensis等进行联合建树分析,对湖北贝母植物的系统位置进行了探讨,为湖北贝母药材的安全使用提供分子证据。结果显示,分子系统树上,3种贝母各自的居群聚为一支,之后天目贝母与安徽贝母聚为一支,最后与湖北贝母聚为一支。表明湖北贝母与天目贝母的亲缘关系可能要远于安徽贝母与天目贝母之间的关系,因此不适宜将湖北贝母归并于天目贝母。
基金supported by the Natural Science Foundation of Yunnan Province,as well as by a grant from the Technology Foundation and the National Key Project for Basic Research(973no.2007CB411600).
文摘Genera Lamiophlomis and Paraphlomis were originally separated from genus Phlomis s.1. on the basis of particular morphological characteristics. However, their relationship was highly contentious, as evidenced by the literature. In the present paper, the systematic positions of Lamiophlomis, Paraphlomis, and their related genera were assessed based on nuclear internal transcribed spacer （ITS） and chloroplast rpl16 and trnL-F sequence data using maximum parsimony （MP） and Bayesian methods. and outgroup were sampled. Analyses of both separate In total, 24 species representing six genera of the ingroup and combined sequence data were conducted to resolve the systematic relationships of these genera. The results reveal that Lamiophlomis is nested within Phlomis sect. Phlomoides and its generic status is not supported. With the inclusion ofLamiophlomis rotata in sect. Phlomoides, sections Phlomis and Phlomoides of Phlomis were resolved as monophyletic. Paraphlomis was supported as an independent genus. However, the resolution of its monophyly conflicted between MP and Bayesian analyses, suggesting the need for expended sampling and further evidence.
文摘<正> Eighteen representative species were selected from all the nine sections of Chinese Allium on the basis of the classification of morphology and cytotaxonomy. The trnK and rpL16 gene fragments of chloroplast DNA were amplified from 18 species by PCR method. The two cpDNA fragments were digested by 26 restriction enzymes, and 303 polymorphic restriction sites were found, of which 163 were informative. The restriction site data were analyzed with PAUP (version 3.1.1) and MEGA (version 1.01) as well as PHYLIP. As a result, the genus Allium could be classified into six subgenera. The recognition of Sect. Anguinum in the Flora of China is reasonable, Sect. Rhizirideum, Sect. Haplostemon and Sect. Cepa are not monophyletic. The infrageneric system of this genus was also discussed.
