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肿瘤相关基因在肝细胞癌及癌旁正常肝组织中表达 被引量:4
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作者 吕自力 文剑明 +3 位作者 罗殿中 张文敏 肖刚 张萌 《临床与实验病理学杂志》 CAS CSCD 2004年第6期654-658,共5页
目的 探讨肿瘤相关基因dek、cyclinD1、胰岛素样生长因子Ⅱ (IGF Ⅱ )、GPC3、核糖体蛋白 0 (rpP0 )mRNA在肝细胞癌 (HCC)组织中及癌旁正常肝组织中的表达及其临床意义。方法 应用RT PCR方法检测 32例HCC组织及相应癌旁正常肝组织中de... 目的 探讨肿瘤相关基因dek、cyclinD1、胰岛素样生长因子Ⅱ (IGF Ⅱ )、GPC3、核糖体蛋白 0 (rpP0 )mRNA在肝细胞癌 (HCC)组织中及癌旁正常肝组织中的表达及其临床意义。方法 应用RT PCR方法检测 32例HCC组织及相应癌旁正常肝组织中dek、cyclinD1、IGF Ⅱ、GPC3和rpP0mRNA的表达情况。 结果 dek、cyclinD1、IGF Ⅱ、GPC3、rpP0mRNA在癌组织中阳性表达率为 78 1%、87 5 %、87 5 %、75 0 %和 81 3% ,在癌旁正常肝组织中阳性表达率为 15 6 %、4 0 6 %、 37 5 %、2 1 9%和 31 3% ,差异有显著性 (P <0 0 5 )。dek、cyclinD1、IGF Ⅱ、GPC3、rpP0mRNA在高分化HCC组织中阳性率为89 0 %、6 6 7%、6 6 7%、6 6 7%和 77 8% ,在低分化HCC组织中阳性率为 73 9%、95 7%、95 7%、95 7%和 82 6 % ,差异无显著性 (P >0 0 5 )。在AFP阴性的HCC中其阳性率分别 90 0 %、80 0 %、90 0 %、90 0 %和 90 0 % ,而AFP阳性的HCC这些基因mRNA的阳性率为 72 7%、86 3%、77 3%、90 9%和 6 8 2 % ,差异无显著性意义 (P >0 0 5 )。结论 dek、cyclinD1、IGF Ⅱ、GPC3、rpP0mRNA在HCC组织中呈高表达状态 ,即使是AFP阴性的HCC也呈高表达 ,因此它们可以作为诊断HCC的基因标志 ,而且联合应用时意义更大。 展开更多
关键词 肝肿瘤 DEK 细胞周期蛋白D1 胰岛素样生长因子Ⅱ GPC3 rpp0 逆转录聚合酶链反应
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Expression and significance of tumor-related genes in HCC 被引量:9
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作者 Zi-LiLü Dian-ZhongLuo Jian-MingWen 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第25期3850-3854,共5页
AIM: To investigate the expression and clinical significance of DEK, cyclin D1, insulin-like growth factor Ⅱ (IGF-Ⅱ), glypican 3 (GPC3), ribosomal phosphoprotein 0 (rpPO) mRNA in hepatocellular carcinoma (HCC) and i... AIM: To investigate the expression and clinical significance of DEK, cyclin D1, insulin-like growth factor Ⅱ (IGF-Ⅱ), glypican 3 (GPC3), ribosomal phosphoprotein 0 (rpPO) mRNA in hepatocellular carcinoma (HCC) and its paraneoplastic tissues. METHODS: The expression of mRNAs of DEK, cyclin D1, IGF-Ⅱ, GPC3 and rpP0 mRNA was detected in HCC and its paraneoplastic tissues by multiplex RT-PCR. RESULTS: By the simplex RT-PCR, the overexpression of mRNAs of DEK, cyclin D1, IGF-Ⅱ, GPC3, rpP0 mRNA in HCC and its paraneoplastic tissues was 78.1%, 87.5%, 87.5%, 75.0%, 81.3% and 15.6%, 40.6%, 37.5%, 21.9%, 31.3% respectively (P<0.05). By the multiplex RT-PCR, at least one of the mRNAs was detected in all HCC samples and in 75.0% of paraneoplastic samples (P>0.05). However, all these five mRNAs were found in 68.8% of HCC samples, but only in 9.4% of paraneoplastic tissues (P<0.05). The positive expression of mRNAs of DEK, cyclin D1, IGF-Ⅱ, GPC3, rpP0 in well- and poorly-differentiated HCC was 89.0%, 66.7%, 66.7%, 66.7%, 77.8% and 73.9%, 95.7%, 95.7%, 95.7%, 82.6%, respectively (P>0.05). The expression of these genes in HCCs with α-feto protein (AFP) negative and positive was 90.0%, 80.0%, 90.0%, 90.0%, 90.0% and 72.7%, 86.3%, 77.3%, 90.9%, 68.2% respectively (P>0.05). CONCLUSION: The expression of DEK, cyclin D1, IGF-Ⅱ, GPC3, rpP0 mRNA in HCC is much higher in HCC than in its paraneoplastic tissues. Multiplex RT-PCR assay is an effective, sensitive, accurate, and cost-effective diagnostic method of HCC. 展开更多
关键词 HCC DEK Cyclin D1 IGF-11 GPC3 rpp0
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