Differential distribution of PINK1 and Parkin in the primate brain implies distinct roles

The vast majority of in vitro studies have demonstrated that PINK1 phosphorylates Parkin to work together in mitophagy to protect against neuronal degeneration.However,it remains largely unclear how PINK1 and Parkin are expressed in mammalian brains.This has been difficult to address because of the intrinsically low levels of PINK1 and undetectable levels of phosphorylated Parkin in small animals.Understanding this issue is critical for elucidating the in vivo roles of PINK1 and Parkin.Recently,we showed that the PINK1 kinase is selectively expressed as a truncated form(PINK1–55)in the primate brain.In the present study,we used multiple antibodies,including our recently developed monoclonal anti-PINK1,to validate the selective expression of PINK1 in the primate brain.We found that PINK1 was stably expressed in the monkey brain at postnatal and adulthood stages,which is consistent with the findings that depleting PINK1 can cause neuronal loss in developing and adult monkey brains.PINK1 was enriched in the membrane-bound fractionations,whereas Parkin was soluble with a distinguishable distribution.Immunofluorescent double staining experiments showed that PINK1 and Parkin did not colocalize under physiological conditions in cultured monkey astrocytes,though they did colocalize on mitochondria when the cells were exposed to mitochondrial stress.These findings suggest that PINK1 and Parkin may have distinct roles beyond their well-known function in mitophagy during mitochondrial damage.

Tree failure modes influenced by the characteristics of tree and its root distribution caused by debris flows

Forests play an important role in controlling the formation and movement processes of debris flows.They contribute to soil stabilization,regulation of soil water content,and act as robust structures impeding the downstream progression of debris flows.On the positive side,trees,to some extent,can intercept debris flows and effectively mitigate their velocity by increasing flow resistance.On the negative side,trees may suffer damage from debris-flow hazards,characterized by the generation of substantial quantities of wood fragments and consequential ramifications such as river channel blockage,resulting in backwater rise.In extreme cases,this blockage collapse can lead to instantaneous discharge amplification,thereby adversely impacting urban safety and impeding sustainable development.Therefore,in order to grasp the effects of tree characteristics on tree failure modes,the tree failure modes and corresponding parameters,diameters at breast height(DBH)and root-soil plate size,were identified and recorded through the post-event field investigation in Keze Gully,a region prone to debrisflow events in Sichuan,China,respectively.To investigate the impact of spatial variability in tree root distribution on tree failure modes,the root crosssectional area ratio(RAR),root density(RD),root length density(RLD)and soil detachment rate(SDR)were obtained.The findings indicated that:(1)Tree characteristics reflect the interactions of debris flows and trees,and influence the tree failure modes ultimately.The root distribution characteristics influence the size and shape of the root-soil plate to affect the resistance of trees.(2)Compared to burial and abrasion,stem breakage and overturning are the predominant modes of tree failure in debris-flow hazards.Trees with a smaller DBH primarily experience stem breakage and bending,and trees with a larger DBH mostly experience overturning.(3)The root-soil plate shapes of overturned trees,affected by the root architecture and root growth range,are generally semielliptical or semicircular,and the horizontal and vertical radii increase with DBH,but the correlation between the root-soil plate’s breadth-depth ratio and DBH is low.(4)The biomass and RAR decrease with distance.The RAR distribution exhibit the order of upslope direction>downslope direction>lateral direction.The coarse root biomass significantly increases with DBH,but no clear trend in fine root biomass.(5)The roots can significantly enhance the soil erosion resistance,but the erosion resistance of coarse roots is not as significant as that of fine roots.The erosion resistance increases with DBH,and follows the order of upslope direction>downslope direction>lateral direction.The results could provide new insights into the influences of tree and root distribution characteristics on tree failure modes during debris flows.

Effect of urbanization on the abundance and distribution of Tree Sparrows (Passer montanus) in Beijing

With rapid urbanization occurring throughout China,the existence of Tree Sparrows (Passer montanus) in big cities is likely to be affected by a decrease in habitat and food availability.Can the urban Tree Sparrow adapt to these changes? To elucidate this question,we studied the effect of urbanization on the abundance and distribution of Tree Sparrows in Beijing.We found the abundance of the Tree Sparrow negatively correlated with an urbanization score.Sparrow abundance was very low in residential areas with high-rise buildings,commercial centers and main roads,while their numbers were significantly higher in parks,university campuses,low building residential and suburban areas.Environmental factors within the 50 m and 200 m scales were most suitable in predicting the distribution of Tree Sparrows during winter,while factors within 50 m and 400 m scales are suitable during the breeding season.During winter,the number of conifer trees and pedestrians were the major factors at the 50 m scale,while the area of high-rise buildings and vegetation become the predominant factors on a 200 m scale.Alternatively,during the breeding season the area of low buildings and the number of conifers and pedestrians were the main factors on the 50 m scale while the area of high-rise buildings and vegetation remained the most important factors on the 400 m scale.These results indicate that highly urbanized areas are not suitable habitats for the Tree Sparrow,although this species can adapt to human environments.Food and nest sites for urban birds should be considered in urban planning of big cities in developing countries.

Neural stem cell-derived exosomes promote mitochondrial biogenesis and restore abnormal protein distribution in a mouse model of Alzheimer's disease

Mitochondrial dysfunction is a hallmark of Alzheimer’s disease.We previously showed that neural stem cell-derived extracellular vesicles improved mitochondrial function in the cortex of AP P/PS1 mice.Because Alzheimer’s disease affects the entire brain,further research is needed to elucidate alterations in mitochondrial metabolism in the brain as a whole.Here,we investigated the expression of several important mitochondrial biogenesis-related cytokines in multiple brain regions after treatment with neural stem cell-derived exosomes and used a combination of whole brain clearing,immunostaining,and lightsheet imaging to clarify their spatial distribution.Additionally,to clarify whether the sirtuin 1(SIRT1)-related pathway plays a regulatory role in neural stem cell-de rived exosomes interfering with mitochondrial functional changes,we generated a novel nervous system-SIRT1 conditional knoc kout AP P/PS1mouse model.Our findings demonstrate that neural stem cell-de rived exosomes significantly increase SIRT1 levels,enhance the production of mitochondrial biogenesis-related fa ctors,and inhibit astrocyte activation,but do not suppress amyloid-βproduction.Thus,neural stem cell-derived exosomes may be a useful therapeutic strategy for Alzheimer’s disease that activates the SIRT1-PGC1αsignaling pathway and increases NRF1 and COXIV synthesis to improve mitochondrial biogenesis.In addition,we showed that the spatial distribution of mitochondrial biogenesis-related factors is disrupted in Alzheimer’s disease,and that neural stem cell-derived exosome treatment can reverse this effect,indicating that neural stem cell-derived exosomes promote mitochondrial biogenesis.

Environmental Correlates of Distribution of the 25 Broad-leaved Tree Species Indigenous to Guangdong Province,China

Twenty-five tree species indigenous to Guangdong Province were chosen in this study to portray their distribution patterns in relation to environmental factors. Both data of species distribution and environmental factors were tabulated based on a digitized map of Guangdong Province gridded at 0.5° latitude × 0.5° longitude. Grid-based diversity was mapped using DMAP, a distribution mapping program, and horizontal patterns were assessed using Kruskal-Wallis tests. The diversity center of the indige- nous tree species under study is located north of 23° N. These tree species exhibit significant latitudinal variation (P = 0.007 4), but no significant longitudinal difference (P = 0.052 2). Non-metric Multidimensional Scaling (NMS) identified five different ecological species groups, while Canonical Correspondence Analysis (CCA) showed the distribution of tree species along each of the five envi- ronmental gradients. An understanding of the environmental correlates of distribution patterns has great implication for the introduc- tion of the indigenous tree species for afforestation.

Vertical Distribution and Seasonal Dynamics of Fine Root Parameters for Apple Trees of Different Ages on the Loess Plateau of China

The vertical distribution pattern and seasonal dynamics of fine root parameters for the apple trees of different ages （3, 10, 15, and 20 years old） on the Loess Plateau of China were studied. Soil coring method was used to determine the vertical distribution and seasonal dynamics of fine roots at different root radial distances （1.0, 1.5, and 2.0 m from the main tree trunk）. The fine root biomass density （FRD）, fine root length density （RLD）, and specific root length （SRL）, as well as soil water content and soil temperature were also measured. The FRD and RLD for the 10, 15, and 20 years old trees reached peak values in the 20-30 cm soil layer. For the 3 years old tree, the highest FRD and RLD were observed in the 10-20 cm soil layer. The FRD and RLD decreased with increased soil depth from the 10-20 or 20-30 cm soil layer for all age apple trees. The SRL declined with the increase of tree age. The FRD at the 1.0 m radial distance from the main tree trunk was higher than that at other radial distances in the 3 and 10 years old orchard. However, in the 15 and 20 years old orchards, especially the 20 years old orchard, the FRD at the 2.0 m radial distance was nearly equal to or higher than that at the 1.0 and 1.5 m radial distances. For all the root radiuses or the tree ages, the FRD, RLD, and SRL were the highest in spring and the lowest in autumn. The age of an apple tree does not affect the vertical distribution pattern but the biomass of fine roots and the SRL. Radial distance affects the root horizontal distribution of 3 and 10 years old trees but the 15 and 20 years old trees. Additionally, effects of soil temperature and soil moisture on fine root distribution or seasonal dynamics are not significant.

基于LSM-Tree的分布式数据库异步融合机制研究与实现

信息技术的不断发展,使得分布式数据库成为研究热点。由于NoSQL架构的分布式数据库对SQL支持有限且在事务处理及一致性方面存在缺陷,基于LSM-Tree的NewSQL数据库逐渐成为应用的主流,例如TiDB、OceanBase等。分布式LSM-Tree的存储架构将数据分为基线数据与增量数据,通过合并操作将不同分区的增量数据与基线数据不断融合,并存储在磁盘,从而减少内存压力。但合并会占用大量系统资源,严重影响系统可用性。因此提出了一种基于LSM-Tree架构的异步融合机制,通过细分合并流程,将数据融合异步化,有效地缩短了单次数据合并的时间。实验表明,提出的异步融合机制可显著缩短数据合并时间,提高系统在高频写入场景下的鲁棒性和可用性。

Community ecology and spatial distribution of trees in a tropical wet evergreen forest in Kaptai national park in Chittagong Hill Tracts, Bangladesh

We investigated the floristic composition, woody species diversity and spatial distribution of trees in a tropical wet evergreen forest in Kaptai national park in Chittagong Hill Tracks, Bangladesh. We recorded 25 families, 37 genera, 40 species and 1771 woody individuals in a 0.09 ha plot. Euphorbiaceae and Moraceae were the most species- rich families, and Castanopsis, Ficus and Terminaliawere the most species-rich genera. Bursera serrata Wall. ex Colebr. was the dominant species in terms of highest importance value （13%）. Trema orientalis （L.） B1 was typically a light demanding species as it appeared in the top can- opy with only one individual having the seventh highest IV, but had no regeneration. The expected maximum number of species （Smax） was 140, indicating that many species may invade the forest as the Sm~ is greater than the recorded total number of species. The nature of the disappear- ance and appearance of species in the present forest reflects instability of floristic composition. The values of Shannon＇s index H＇ and Pielou＇s index J＇ （evenness） were 3.36 bit and 0.63, respectively. These values show moderately high species diversity as compared to other subtropical forests in the tropics. In addition, a sample area of 200 m2 in this forest would be sufficient for measuring the diversity indices H＂ and ,/＇, whereas the trend of J＇may indicate the rate of equality of individuals among the different species decreased with increasing area. The distribu- tion pattern for the total stand was completely random. However, the dominant species showed aggregate distribution for small areas, but random distribution for large areas. The spatial association between species showed that the strongest positive interspecific association occurred between Streblus asper Lour. and Castanea indica Roxh. （09 = 0.51）. As a whole, most species were weakly associated with each other, of which 58% species associations were completely negative. The result of cluster analysis showed that species pairs were spatially independent at all or most small clusters; stands of species from all clusters are mosa- ics of complete habitat and pioneer habitat. All patches in this forest community have similar habitat and regeneration niches, which could be a phenomenon for a young growth forest. Thus, the existence of habitat and regeneration niches may be an important factor in the maintenance of diversity in this forest.

Angular Distribution of Element Contents in Tree Rings and the Environmental Information

Element contents of tree rings and soils near tree roots collected from Deodar cedar (Cedrus deodara (Roxb.) G. Don) and Masson pine (Picks massoniana lamb.) were determined to study the relationship between the angular distribution of element contents in tree rings and the environmental information. The chemical composition and properties of soils are very much complicated, which leads to the non-uniform distribution of the element contents in tree rings. The statistical multi-variable regression method was used to got the information of the tree-centered distribution of element contents in the environment (soil) (C’), C’(z, θj ), from the distribution of element contents in tree rings (C), C(Z, θi), which depends on the plane azimuth angle (θi), i. e., C=C(Z,θi), where Z is the atomic number of the element, with a satisfactory result,though this study is only a primary one.

Tree structure and diversity shape the biomass of primary temperate mountain forests

Primary forests are spatially diverse terrestrial ecosystems with unique characteristics,being naturally regenerative and heterogeneous,which supports the stability of their carbon storage through the accumulation of live and dead biomass.Yet,little is known about the interactions between biomass stocks,tree genus diversity and structure across a temperate montane primary forest.Here,we investigated the relationship between tree structure(variability in basal area and tree size),genus-level diversity(abundance,tree diversity)and biomass stocks in temperate primary mountain forests across Central and Eastern Europe.We used inventory data from726 permanent sample plots from mixed beech and spruce across the Carpathian Mountains.We used nonlinear regression to analyse the spatial variability in forest biomass,structure,and genus-level diversity and how they interact with plot-level tree age,disturbances,temperature and altitude.We found that the combined effects of genus and structural indices were important for addressing the variability in biomass across different spatial scales.Local processes in disturbance regimes and uneven tree age support forest hete rogeneity and the accumulation of live and dead biomass through the natural regeneration,growth and decay of the forest ecosystem.Structural complexities in basal area index,supporte d by genus-level abundance,positively influence total biomass stocks,which was modulated by tree age and disturbances.Spruce forests showed higher tree density and basal area than mixed beech forests,though mixed beech still contributes significantly to biomass across landscapes.Forest heterogeneity was strongly influenced by complexities in forest composition(tree genus diversity,structure).We addressed the importance of primary forests as stable carbon stores,achieved through structure and diversity.Safeguarding such ecosystems is critical for ensuring the stability of the primary forest,carbon store and biodiversity into the future.

Application of Digital Photogrammetry to Measure Distribution of Tree Postions

The application of digital photogrammetry to measure distribution of tree positions with stereo image couple is introduced in detail, and the procedure of stereo vision applied in forestry environment is explored. Nonlinear error in measure model is adopted in the camera calibration; the interactive correlation matching is used under constraint of epipolar line and edge of tree detected by Canny operator. Results prove that application of digital photogrammetry technology to measure distribution of tree positions can meet demand of precision in experimental conditions.

Spatiotemporal Distributions of Bacterioplankton Communities in the Qiantang River(Hangzhou Section),China

The spatial and temporal distribution of bacterioplankton communities plays a vital role in understanding the ecological dynamics and health of aquatic ecosystems.In this study,we conducted a comprehensive investigation of the bacterioplankton communities in the Qiantang River(Hangzhou section).Water samples were collected quarterly from seven sites over a one-year period.Physical and chemical parameters,including dissolved oxygen(DO),water temperature(WT),chemical oxygen demand(COD),nitrite(NO_(2)^(-)),active phosphate(PO_(4)^(3-))and other indices were determined.In this study,theαdiversity,βdiversity and abundance differences of bacterial communities were investigated using 16S rRNA high-throughput sequencing analysis.The spatial and temporal distribution characteristics and main driving factors of the bacterioplankton community structure and diversity were discussed.The results showed that a total of 57 phyla were detected in the bacterioplankton community,among which Proteobacteria and Actinomycetes were the two dominant groups with the highest relative abundance.The results of PCoA based on Bray-Curtis distance showed that the sampling season had a slightly greater effect on the changes in bacterioplankton community structure in the Qiantang River.Mantel and partial Mantel test showed that environmental variables(Mantel r=0.6739,P<0.0001;partial Mantel r=0.507,P=0.0001)were more important than geographical distance(Mantel r=0.5322,P<0.001;partial Mantel r=0.1563,P=0.001).The distance attenuation model showed that there was significant distance attenuation in all four seasons,and the maximum limit of bacterial community diffusion was found in spring.RDA analysis showed that nine environmental factors,including COD,WT and DO,significantly affected community distribution(P<0.05).This study provides valuable insights into the spatial and temporal distribution characteristics of bacterioplankton communities,shedding light on their ecological roles in the Qiantang River.The information obtained can guide future research on the interactions between bacterioplankton and their environment,enabling the development of effective conservation strategies and sustainable management practices for aquatic ecosystems.

New indices to balanceα-diversity against tree size inequality

The number and composition of species in a community can be quantified withα-diversity indices,including species richness(R),Simpson’s index(D),and the Shannon-Wiener index(H΄).In forest communities,there are large variations in tree size among species and individu-als of the same species,which result in differences in eco-logical processes and ecosystem functions.However,tree size inequality(TSI)has been largely neglected in studies using the available diversity indices.The TSI in the diameter at breast height(DBH)data for each of 99920 m×20 m forest census quadrats was quantified using the Gini index(GI),a measure of the inequality of size distribution.The generalized performance equation was used to describe the rotated and right-shifted Lorenz curve of the cumulative proportion of DBH and the cumulative proportion of number of trees per quadrat.We also examined the relationships ofα-diversity indices with the GI using correlation tests.The generalized performance equation effectively described the rotated and right-shifted Lorenz curve of DBH distributions,with most root-mean-square errors(990 out of 999 quadrats)being<0.0030.There were significant positive correlations between each of threeα-diversity indices(i.e.,R,D,and H’)and the GI.Nevertheless,the total abundance of trees in each quadrat did not significantly influence the GI.This means that the TSI increased with increasing spe-cies diversity.Thus,two new indices are proposed that can balanceα-diversity against the extent of TSI in the com-munity:(1−GI)×D,and(1−GI)×H’.These new indices were significantly correlated with the original D and H΄,and did not increase the extent of variation within each group of indices.This study presents a useful tool for quantifying both species diversity and the variation in tree sizes in forest communities,especially in the face of cumulative species loss under global climate change.

The distribution and frequency of damage to roadside trees in low-volume road construction

We investigated the distribution and frequency of damage to tree stands adjacent to low-volume roads according to the type of hillside materials involved（soil or rock） and hillside gradient in mountainous forests of northern Iran. A total of 80 plots were systematically and randomly sampled to record damaged trees（bending,crushing and wounding） by class of hillside gradient and materials at the edge of road. Tree wounding and crushing at rock slopes was significantly greater than at hillsides with a mix of clay soil（p ／ 0.05）. Damage on hillsides with slope gradients[45% were 2, 8.5 and 2.3 times more frequent than on hillsides with slope gradient／15% for bending, crushing and wounding, respectively. The damage distribution varied according by type and the most frequent damage was tree wounding（p ／ 0.05）. The damage distribution was measured at distances of 4, 5 and 8 m from the road fillslope for tree bending, crushing and wounding, respectively. Using hydraulic excavators and physical barriers（wooden obstruction and synthetic holder） during earthworks for road construction could reduce these damage.

Historical and current climates affect the spatial distribution of herbivorous tree insects in China

Historical and current climate impacts reshape the evolutionary trajectory and ecological dynamics of entire vegetative communities,which can drive insect species distribution.Understanding the spatial distribution of insects can enhance forest management effectiveness.The effects of historical and current climates in the spatial distribution of herbivorous tree insects in China were explored.A species distribution model simulated insect spatial distribution based on 596 species and the distribution probability and richness of these species were assessed in forest ecoregions.The explanatory power of the historical climate was stronger than that of the current climate,particularly historical annual precipitation and annual mean temperatures,for the distribution of herbivorous insects.Under both historical and current climatic conditions,herbivorous tree insects were and are mainly distributed in the North China Plain and the middle and lower reaches of the Yangtze River Plain,namely in the Huang He Plain mixed forests,Changjiang Plain evergreen forests,and Sichuan Basin evergreen broadleaf forests.The Yunnan-Guizhou Plateau and northeast China are regions with large impact differences between historical and current climates.The findings of this study provide valuable insights into herbivorous insect responses to sustained climate change and may contribute to long-term biodiversity conservation activities.

Study on Influence of Trees around 10 kV Distribution Line

Considering the complexity of the geographical surroundings and the height of 10 kV distribution line, the impact of trees on the lightning performance can not be ignored. A model of lightning position judgment and overvoltage calculation based on the concept of striking distance is built. With the theory of orthogonal test, the main factors of trees' influence on lightning performance are figured out. The results indicate that the location of trees is the main factor. In practical engineering, suitable management of vegetation can improve the lightning performance and reduce the lightning failure of 10 kV distribution line.

Spatial Distribution Pattern of Batocera horsfieldi Attacking Ash Trees in Different Forests

[Objective]The paper was to study the spatial distribution of Batocera horsfieldi in different ash forests.[Method]The number of excretory holes,eclosion holes and channels of B.horsfieldi in five different stand types of ash forests(patch forest,yard greening forest,highway street forest,urban street forest and yard street forest)was investigated,and their spatial distribution characteristics were statistically analyzed.[Result]The aggregation index,Iwao regression equation and Taylor power exponential equation were calculated.The excretory holes,eclosion holes and channels of B.horsfieldi attacking five different stand types of ash forests showed aggregated distribution,but the aggregation index parameters,αandβof Iwao regression equation,lga and b of Taylor power exponential equation varied with different stand types.[Conclusion]B.horsfieldi has caused serious harms to ash tree which is an important landscaping tree in the Yellow River delta area,and it is of guiding value to sampling investigation and prevention and control of B.horsfieldi.

Evaluation of Probability Distribution Functions for Modeling Forest Tree Diameters on Agricultural Landscapes in Ogun State, Nigeria

The use of probability distribution functions for describing tree diameter at breast height provides useful information for forest resource evaluation and quantification. A series of probability distribution functions have been widely developed and applied for managing forest trees in conventional forest reserves without much consideration for trees outside forest reserves. The aim of this study is to evaluate and propose a suitable probability distribution function for trees in Agricultural landscapes. The study examined 3-parameter lognormal, Lognormal, 3-parameter Gamma, Gamma, 3-parameter Weibull and Weibull distribution functions, using the Maximum Likelihood method for fitting tree diameter at breast height. Three hundred and thirty-two temporary farmlands were randomly selected from which stem diameter of all living trees, with diameter ≥ 10.0 cm, were measured. Results of the statistical analysis showed that the 3-parameter lognormal distribution gave a superior description of the stem diameter with the least values of Anderson Darling (1.627) and Akaike Information Criterion (5962.0) statistics. Hence, the 3-parameter lognormal distribution function was found suitable for the stem diameter of trees in Agricultural landscapes in the study area.

Stability of weighted spectral distribution in a pseudo tree-like network model

The comparison of networks with different orders strongly depends on the stability analysis of graph features in evolving systems. In this paper, we rigorously investigate the stability of the weighted spectral distribution（i.e., a spectral graph feature） as the network order increases. First, we use deterministic scale-free networks generated by a pseudo treelike model to derive the precise formula of the spectral feature, and then analyze the stability of the spectral feature based on the precise formula. Except for the scale-free feature, the pseudo tree-like model exhibits the hierarchical and small-world structures of complex networks. The stability analysis is useful for the classification of networks with different orders and the similarity analysis of networks that may belong to the same evolving system.

Research on Monitoring and Early-warning Standards of Ancient and Famous Trees Protection in the Humble Administrator's Garden

Based on the current situation and symptoms of the trees' growth in the Humble Administrator's Garden,this paper put forward corresponding monitoring and early-warning standards and technical measures of the ancient and famous trees protection in the Humble Administrator's Garden specifically.The aim of doing this is to establish a scientific basis for the protection of the ancient and famous trees in the Humble Administrator's Garden by setting up systematic fundamental data,dynamic protection standard grades and technique measures of protecting the trees.The main symptom of trees in the Humble Administrator's Garden is the erosion and decay of the tree trunks.Fifteen tree trunks need technical protection,which holds 65.22% of the total sum of trees in the Humble Administrator's Garden.Therefore,much more emphasis should be paid in strengthening technical protection procedures of monitoring and early warning of the tree trunks in the future protection of the ancient and famous trees in the garden.Besides,the rejuvenation technique of rooting zone and rooting system,tree pruning technique as well as tree supporting measures according to the specific condition and symptom of the trees should be concerned with in order to protect the ancient and famous trees in the Humble Administrator's Garden in a more scientific and effective way.