An improved algebraic reconstruction technique（ART） combined with tunable diode laser absorption spectroscopy（TDLAS） is presented in this paper for determining two-dimensional（2D） distribution of H2O concentrati...An improved algebraic reconstruction technique（ART） combined with tunable diode laser absorption spectroscopy（TDLAS） is presented in this paper for determining two-dimensional（2D） distribution of H2O concentration and temperature in a simulated combustion flame.This work aims to simulate the reconstruction of spectroscopic measurements by a multi-view parallel-beam scanning geometry and analyze the effects of projection rays on reconstruction accuracy.It finally proves that reconstruction quality dramatically increases with the number of projection rays increasing until more than 180 for 20 × 20 grid,and after that point,the number of projection rays has little influence on reconstruction accuracy.It is clear that the temperature reconstruction results are more accurate than the water vapor concentration obtained by the traditional concentration calculation method.In the present study an innovative way to reduce the error of concentration reconstruction and improve the reconstruction quality greatly is also proposed,and the capability of this new method is evaluated by using appropriate assessment parameters.By using this new approach,not only the concentration reconstruction accuracy is greatly improved,but also a suitable parallel-beam arrangement is put forward for high reconstruction accuracy and simplicity of experimental validation.Finally,a bimodal structure of the combustion region is assumed to demonstrate the robustness and universality of the proposed method.Numerical investigation indicates that the proposed TDLAS tomographic algorithm is capable of detecting accurate temperature and concentration profiles.This feasible formula for reconstruction research is expected to resolve several key issues in practical combustion devices.展开更多
The Computer Tomography(CT)method is used for remote sensing the Earth’s plasmasphere.One challenge for image reconstruction is insufficient projection data,mainly caused by limited projection angles.In this study,we...The Computer Tomography(CT)method is used for remote sensing the Earth’s plasmasphere.One challenge for image reconstruction is insufficient projection data,mainly caused by limited projection angles.In this study,we apply the Algebraic Reconstruction Technique(ART)and the minimization of the image Total Variation(TV)method,with a combination of priori knowledge of north–south symmetry,to reconstruct plasmaspheric He+density from simulated EUV images.The results demonstrate that incorporating priori assumption can be particularly useful when the projection data is insufficient.This method has good performance even with a projection angle of less than 150 degrees.The method of our study is expected to have applications in the Soft X-ray Imager(SXI)reconstruction for the Solar wind–Magnetosphere–Ionosphere Link Explorer(SMILE)mission.展开更多
目的:克隆人类S期激酶相关蛋白2(Skp2)基因,构建其表达质粒,为今后进一步研究Skp2的生物学功能提供基础。方法:采用RT-PCR技术扩增人类Skp2 eDNA全长序列,运用DNA重组技术将其重组于pcDNA3.1/myc- His A质粒,构建Skp2表达质粒。结果:通...目的:克隆人类S期激酶相关蛋白2(Skp2)基因,构建其表达质粒,为今后进一步研究Skp2的生物学功能提供基础。方法:采用RT-PCR技术扩增人类Skp2 eDNA全长序列,运用DNA重组技术将其重组于pcDNA3.1/myc- His A质粒,构建Skp2表达质粒。结果:通过酶切电泳分析及DNA测序分析证实,实验成功构建了Skp2表达质粒。结论:Skp2表达质粒pcDNA3.1/mye-His A-Skp2构建成功,为进一步研究Skp2的生物学功能及其在肿瘤发生、发展、诊断和预后中的作用奠定了基础。展开更多
This paper presents a novel approach for assessing the precision of the wet refractivity field using BDS （BeiDou navigation satellite system） simulations only,GPS,and BDS＋GPS for the Shenzhen and Hongkong GNSS netw...This paper presents a novel approach for assessing the precision of the wet refractivity field using BDS （BeiDou navigation satellite system） simulations only,GPS,and BDS＋GPS for the Shenzhen and Hongkong GNSS network.The simulations are carried out by adding artificial noise to a real observation dataset.Instead of using the δ and σ parameters computed from slant wet delay,as in previous studies,we employ the Bias and RMS parameters,computed from the tomography results of total voxels,in order to obtain a more direct and comprehensive evaluation of the precision of the refractivity field determination.The results show that：（1） the precision of tropospheric wet refractivity estimated using BDS alone （only 9 satellites used） is basically comparable to that of GPS; （2） BDS＋GPS （as of current operation） may not be able to significantly improve the data＇s spatial density for the application of refractivity tomography; and （3） any slight increase in the precision of refractivity tomography,particularly in the lower atmosphere,bears great significance for any applications dependent on the Chinese operational meteorological service.展开更多
基金Project supported by the Young Scientists Fund of the National Natural Science Foundation of China(Grant No.61205151)the National Key Scientific Instrument and Equipment Development Project of China(Grant No.2014YQ060537)the National Basic Research Program,China(Grant No.2013CB632803)
文摘An improved algebraic reconstruction technique（ART） combined with tunable diode laser absorption spectroscopy（TDLAS） is presented in this paper for determining two-dimensional（2D） distribution of H2O concentration and temperature in a simulated combustion flame.This work aims to simulate the reconstruction of spectroscopic measurements by a multi-view parallel-beam scanning geometry and analyze the effects of projection rays on reconstruction accuracy.It finally proves that reconstruction quality dramatically increases with the number of projection rays increasing until more than 180 for 20 × 20 grid,and after that point,the number of projection rays has little influence on reconstruction accuracy.It is clear that the temperature reconstruction results are more accurate than the water vapor concentration obtained by the traditional concentration calculation method.In the present study an innovative way to reduce the error of concentration reconstruction and improve the reconstruction quality greatly is also proposed,and the capability of this new method is evaluated by using appropriate assessment parameters.By using this new approach,not only the concentration reconstruction accuracy is greatly improved,but also a suitable parallel-beam arrangement is put forward for high reconstruction accuracy and simplicity of experimental validation.Finally,a bimodal structure of the combustion region is assumed to demonstrate the robustness and universality of the proposed method.Numerical investigation indicates that the proposed TDLAS tomographic algorithm is capable of detecting accurate temperature and concentration profiles.This feasible formula for reconstruction research is expected to resolve several key issues in practical combustion devices.
基金supported by the National Natural Science Foundation of China(Grant Nos.41904148,41731070,41874175)in part by the Strategic Priority Program on Space Science,Chinese Academy of Sciences(Grant Nos.XDA15017000,XDA15350201,XDA15052500).
文摘The Computer Tomography(CT)method is used for remote sensing the Earth’s plasmasphere.One challenge for image reconstruction is insufficient projection data,mainly caused by limited projection angles.In this study,we apply the Algebraic Reconstruction Technique(ART)and the minimization of the image Total Variation(TV)method,with a combination of priori knowledge of north–south symmetry,to reconstruct plasmaspheric He+density from simulated EUV images.The results demonstrate that incorporating priori assumption can be particularly useful when the projection data is insufficient.This method has good performance even with a projection angle of less than 150 degrees.The method of our study is expected to have applications in the Soft X-ray Imager(SXI)reconstruction for the Solar wind–Magnetosphere–Ionosphere Link Explorer(SMILE)mission.
文摘目的:克隆人类S期激酶相关蛋白2(Skp2)基因,构建其表达质粒,为今后进一步研究Skp2的生物学功能提供基础。方法:采用RT-PCR技术扩增人类Skp2 eDNA全长序列,运用DNA重组技术将其重组于pcDNA3.1/myc- His A质粒,构建Skp2表达质粒。结果:通过酶切电泳分析及DNA测序分析证实,实验成功构建了Skp2表达质粒。结论:Skp2表达质粒pcDNA3.1/mye-His A-Skp2构建成功,为进一步研究Skp2的生物学功能及其在肿瘤发生、发展、诊断和预后中的作用奠定了基础。
基金supported by the National Basic ResearchDevelopment (973) Program of China (Grant No. 2012CB955903)+1 种基金the National Natural Science Foundation of China (Grant No. 20907047 and Grant No. 71373131)National Industry-specific Topics (Grant No.GYHY 201406078)
文摘This paper presents a novel approach for assessing the precision of the wet refractivity field using BDS （BeiDou navigation satellite system） simulations only,GPS,and BDS＋GPS for the Shenzhen and Hongkong GNSS network.The simulations are carried out by adding artificial noise to a real observation dataset.Instead of using the δ and σ parameters computed from slant wet delay,as in previous studies,we employ the Bias and RMS parameters,computed from the tomography results of total voxels,in order to obtain a more direct and comprehensive evaluation of the precision of the refractivity field determination.The results show that：（1） the precision of tropospheric wet refractivity estimated using BDS alone （only 9 satellites used） is basically comparable to that of GPS; （2） BDS＋GPS （as of current operation） may not be able to significantly improve the data＇s spatial density for the application of refractivity tomography; and （3） any slight increase in the precision of refractivity tomography,particularly in the lower atmosphere,bears great significance for any applications dependent on the Chinese operational meteorological service.
