Objective:To investigate the correlation of urine sFlt-1/PLGF ratio with abnormal trophoblast invasion and apoptosis in pregnant women with preeclampsia.Methods:A total of 183 healthy second-trimester pregnant women w...Objective:To investigate the correlation of urine sFlt-1/PLGF ratio with abnormal trophoblast invasion and apoptosis in pregnant women with preeclampsia.Methods:A total of 183 healthy second-trimester pregnant women who received antenatal care in this hospital between December 2015 and December 2017 were selected as the normal control group, and 62 second-trimester pregnant women with preeclampsia who received antenatal care in this hospital during the same period were selected as the preeclampsia group. The difference of sFlt-1/PLGF ratio in the urine was compared between the two groups of pregnant women, and the expression levels of invasion-related genes and apoptosis-related genes in the placental tissues were detected. The Pearson test was adopted to further evaluate the correlation of urine sFlt-1/PLGF ratio with abnormal trophoblast invasion and apoptosis in pregnant women with preeclampsia.Results: Urine sFlt-1/PLGF ratio of preeclampsia group was significantly higher than that in normal control group;Rho, ROCK, Notch-1, MMP9 and LAMA4 mRNA expression in placental tissues of preeclampsia group were lower than those of normal control group whereas CDKN1C mRNA expression was higher than that of normal control group;Bcl-2 and Survivin mRNA expression in placental tissues of preeclampsia group were lower than those of normal control group whereas Bax, Caspase-3 and Caspase-8 mRNA expression were higher than those of normal control group. Correlation analysis showed that the urine sFlt-1/PLGF ratio of pregnant women with preeclampsia was directly correlated with the expression of placental trophoblast invasion-related genes and apoptosis-related genes in pregnant women with preeclampsia.Conclusion: The sFlt-1/PLGF ratio abnormally increases in the urine of pregnant women with preeclampsia, which can further aggravate the abnormal invasion and apoptosis of placental trophoblast cells.展开更多
目的檢測尿蛋白陽性孕婦血清可溶性血管内皮生長因子受體-1(sFlt-1)、胎盤生長因子(PlGF)水平,探討sFlt-1/PlGF比值預測子癇前期及其他不良妊娠結局的實用價值。方法收集2019年10月~2022年11月尿蛋白陽性孕婦80例為觀察組,同期尿蛋白陰...目的檢測尿蛋白陽性孕婦血清可溶性血管内皮生長因子受體-1(sFlt-1)、胎盤生長因子(PlGF)水平,探討sFlt-1/PlGF比值預測子癇前期及其他不良妊娠結局的實用價值。方法收集2019年10月~2022年11月尿蛋白陽性孕婦80例為觀察組,同期尿蛋白陰性的孕婦40例為對照組,檢測兩組孕婦血清中sFlt-1及PlGF水平,統計分析兩組sFlt-1/PlGF比值>38的差異性,受試者工作特徵曲線(Receiver Operating Characteristic curve,ROC)評估sFlt-1/PlGF比值預測不良妊娠結局及子癇前期的診斷價值。結果觀察組及對照組血清sFlt-1/PlGF比值中位數分别為14.66、5.06,觀察組高於對照組,兩組間差異有統計學意義(P<0.05);用sFlt-1/PlGF比值與不良妊娠結局及子癇前期作ROC曲線分析,曲線下面積(Area Under the Curve,AUC)分别為0.90、0.77。結論尿蛋白陽性孕婦血清sFlt-1/PlGF比值,能為子癎前期及其他不良妊娠結局的預測提供參考價值。展开更多
文摘Objective:To investigate the correlation of urine sFlt-1/PLGF ratio with abnormal trophoblast invasion and apoptosis in pregnant women with preeclampsia.Methods:A total of 183 healthy second-trimester pregnant women who received antenatal care in this hospital between December 2015 and December 2017 were selected as the normal control group, and 62 second-trimester pregnant women with preeclampsia who received antenatal care in this hospital during the same period were selected as the preeclampsia group. The difference of sFlt-1/PLGF ratio in the urine was compared between the two groups of pregnant women, and the expression levels of invasion-related genes and apoptosis-related genes in the placental tissues were detected. The Pearson test was adopted to further evaluate the correlation of urine sFlt-1/PLGF ratio with abnormal trophoblast invasion and apoptosis in pregnant women with preeclampsia.Results: Urine sFlt-1/PLGF ratio of preeclampsia group was significantly higher than that in normal control group;Rho, ROCK, Notch-1, MMP9 and LAMA4 mRNA expression in placental tissues of preeclampsia group were lower than those of normal control group whereas CDKN1C mRNA expression was higher than that of normal control group;Bcl-2 and Survivin mRNA expression in placental tissues of preeclampsia group were lower than those of normal control group whereas Bax, Caspase-3 and Caspase-8 mRNA expression were higher than those of normal control group. Correlation analysis showed that the urine sFlt-1/PLGF ratio of pregnant women with preeclampsia was directly correlated with the expression of placental trophoblast invasion-related genes and apoptosis-related genes in pregnant women with preeclampsia.Conclusion: The sFlt-1/PLGF ratio abnormally increases in the urine of pregnant women with preeclampsia, which can further aggravate the abnormal invasion and apoptosis of placental trophoblast cells.
文摘目的檢測尿蛋白陽性孕婦血清可溶性血管内皮生長因子受體-1(sFlt-1)、胎盤生長因子(PlGF)水平,探討sFlt-1/PlGF比值預測子癇前期及其他不良妊娠結局的實用價值。方法收集2019年10月~2022年11月尿蛋白陽性孕婦80例為觀察組,同期尿蛋白陰性的孕婦40例為對照組,檢測兩組孕婦血清中sFlt-1及PlGF水平,統計分析兩組sFlt-1/PlGF比值>38的差異性,受試者工作特徵曲線(Receiver Operating Characteristic curve,ROC)評估sFlt-1/PlGF比值預測不良妊娠結局及子癇前期的診斷價值。結果觀察組及對照組血清sFlt-1/PlGF比值中位數分别為14.66、5.06,觀察組高於對照組,兩組間差異有統計學意義(P<0.05);用sFlt-1/PlGF比值與不良妊娠結局及子癇前期作ROC曲線分析,曲線下面積(Area Under the Curve,AUC)分别為0.90、0.77。結論尿蛋白陽性孕婦血清sFlt-1/PlGF比值,能為子癎前期及其他不良妊娠結局的預測提供參考價值。
