In plant immunity,the mutually antagonistic hormones salicylic acid(SA)and jasmonic acid(JA)are implicated in resistance to biotrophic and necrotrophic pathogens,respectively.Promoters that can respond to both SA and ...In plant immunity,the mutually antagonistic hormones salicylic acid(SA)and jasmonic acid(JA)are implicated in resistance to biotrophic and necrotrophic pathogens,respectively.Promoters that can respond to both SA and JA signals are urgently needed to engineer plants with enhanced resistance to a broad spectrum of pathogens.However,few natural pathogen-inducible promoters are available for this purpose.To address this problem,we have developed a strategy to synthesize dual SA-and JA-responsive promoters by combining SA-and JA-responsive cis elements based on the interaction between their cognate transacting factors.The resulting promoters respond rapidly and strongly to both SA and Methyl Jasmonate(MeJA),as well as different types of phytopathogens.When such a synthetic promoter was used to control expression of an antimicrobial peptide,transgenic plants displayed enhanced resistance to a diverse range of biotrophic,necrotrophic,and hemi-biotrophic pathogens.A dual-inducible promoter responsive to the antagonistic signals auxin and cytokinin was generated in a similar manner,confirming that our strategy can be used for the design of other biotically or abiotically inducible systems.展开更多
热激蛋白70(HSP70,heat shock protein 70)广泛参与胁迫环境的响应,在诱发人体肿瘤细胞凋亡,增强肿瘤的免疫原性中起着重要作用。然而,植物HSP70的生理功能研究起步较晚,最近的研究发现植物HSP70在细胞内主要参与新生肽的折叠与成熟、...热激蛋白70(HSP70,heat shock protein 70)广泛参与胁迫环境的响应,在诱发人体肿瘤细胞凋亡,增强肿瘤的免疫原性中起着重要作用。然而,植物HSP70的生理功能研究起步较晚,最近的研究发现植物HSP70在细胞内主要参与新生肽的折叠与成熟、损伤蛋白的降解和蛋白运输;植物HSP70在非生物胁迫环境的应答、抗病性及植物发育中起着重要作用。本文从分子生物学角度,系统综述了植物HSP70分子作用机理研究的进展,以及在提高植物抗逆性方面的作用,以期为基因工程方法改良作物抗性提供参考。展开更多
基金supported by the National Natural Science Foundation of China(31071463)Chongqing Foundation for leaders of disciplines in science(cstc2014kjcxljrc005)the Chongqing Research Program of Basic Research and Frontier Technology(cstc2017jcyjB0316).
文摘In plant immunity,the mutually antagonistic hormones salicylic acid(SA)and jasmonic acid(JA)are implicated in resistance to biotrophic and necrotrophic pathogens,respectively.Promoters that can respond to both SA and JA signals are urgently needed to engineer plants with enhanced resistance to a broad spectrum of pathogens.However,few natural pathogen-inducible promoters are available for this purpose.To address this problem,we have developed a strategy to synthesize dual SA-and JA-responsive promoters by combining SA-and JA-responsive cis elements based on the interaction between their cognate transacting factors.The resulting promoters respond rapidly and strongly to both SA and Methyl Jasmonate(MeJA),as well as different types of phytopathogens.When such a synthetic promoter was used to control expression of an antimicrobial peptide,transgenic plants displayed enhanced resistance to a diverse range of biotrophic,necrotrophic,and hemi-biotrophic pathogens.A dual-inducible promoter responsive to the antagonistic signals auxin and cytokinin was generated in a similar manner,confirming that our strategy can be used for the design of other biotically or abiotically inducible systems.
文摘热激蛋白70(HSP70,heat shock protein 70)广泛参与胁迫环境的响应,在诱发人体肿瘤细胞凋亡,增强肿瘤的免疫原性中起着重要作用。然而,植物HSP70的生理功能研究起步较晚,最近的研究发现植物HSP70在细胞内主要参与新生肽的折叠与成熟、损伤蛋白的降解和蛋白运输;植物HSP70在非生物胁迫环境的应答、抗病性及植物发育中起着重要作用。本文从分子生物学角度,系统综述了植物HSP70分子作用机理研究的进展,以及在提高植物抗逆性方面的作用,以期为基因工程方法改良作物抗性提供参考。
