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Effect of Exogenous Gibberellin on Wheat Seed Germination under Salt Stress
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作者 Tureding Abudurousuli Baotong LI Aizemaiti Tuerhong 《Asian Agricultural Research》 2023年第8期63-66,共4页
[Objectives]In this experiment,wheat seeds were treated with different concentrations of gibberellin and different concentrations of salt solution to study the change of germination index of wheat seeds.[Methods]The g... [Objectives]In this experiment,wheat seeds were treated with different concentrations of gibberellin and different concentrations of salt solution to study the change of germination index of wheat seeds.[Methods]The germination rate,germination potential and germination index of wheat seeds were measured by routine methods,and the effect of exogenous gibberellin on germination of wheat seeds under salt stress was observed.[Results]The germination rate,germination potential and germination index of wheat seeds under salt stress were significantly increased after exogenous treatment of 0.25 and 0.50 g/L gibberellin within the range of salt concentration gradient.However,when the concentration of gibberellin was too high,it would inhibit the germination of seeds.[Conclusions]Appropriate concentration of gibberellin can effectively alleviate the stress caused by salt on wheat seed germination.In this experiment,the best concentration of gibberellin to alleviate salt stress was 0.25 g/L. 展开更多
关键词 GIBBERELLIN salt stress Wheat seed Seed germination
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Effect of Seed Soaking with Exogenous Proline on Seed Germination of Rice Under Salt Stress 被引量:2
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作者 Liu Hua-long Sha Han-jing +3 位作者 Wang Jing-guo Liu Yang Zou De-tang Zhao Hong-wei 《Journal of Northeast Agricultural University(English Edition)》 CAS 2014年第3期1-6,共6页
To explore the germination mechanism of salt-stressed rice improved by exogenous proline, and provide a theoretical basis to rice direct sowing technology for salinized soil, the effects of soaking with proline on ger... To explore the germination mechanism of salt-stressed rice improved by exogenous proline, and provide a theoretical basis to rice direct sowing technology for salinized soil, the effects of soaking with proline on germination status, amylase activity and isoenzyme were studied in this paper. The results showed that germination status including germination energy(GE), germination rate(GR), relative germination energy(RGE) and relative germination rate(RGR) significantly decreased as the same as the activities of alpha-amylase, beta-amylase and the total amylase under salt stress. Soaking with exogenous proline improved the germination status of rice under salt stress. Moreover, GE and RGE of salt-stressed rice were improved with increasing of proline concentration at the range of 5-45 mmol ·L-1. Soaking with 15 mmol ·L-1 and 30 mmol ·L-1 proline significantly improved the amylase activities(e.g. alpha-amylase, beta-amylase and total amylase) of rice under salt stress. Salt stress inhibited the express of beta-amylase isoenzyme temporarily, but had few impacts on alpha-amylase isozyme. Soaking with 30 mmol ·L-1 proline brightened District I and increased the width of 'i' brand in District II of alpha-amylase isoenzyme, but had few impacts on beta-amylase isoenzyme. In a word, soaking with proline could effectively alleviate the inhibitory effects of salt stress on seed germination. 展开更多
关键词 exogenous proline salt stress RICE germination AMYLASE
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Germination Behaviour of a Perennial Amaranthaceae of North Western Algeria(Oran):Arthrocnemum glaucum(Del.)Ung.Respectively Submitted to Thermal Factor and Salt Stress
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作者 Amel Sari-Ali Taleb-Bendiab Amel Bensouna +2 位作者 Faical Hassani Tawfik Ferouani Hassiba Stambouli 《Open Journal of Ecology》 2015年第11期544-551,共8页
In light of the importance of seeds germinative phase in the progress of later stages of development of any particular plant species in semi-arid and arid area, it is essential to study the germinative behavior and to... In light of the importance of seeds germinative phase in the progress of later stages of development of any particular plant species in semi-arid and arid area, it is essential to study the germinative behavior and to evaluate the tolerance of a perennial halophyte of Amaranthacaea family, Arthrocnemum glaucum (Del.) Ung. during this crucial phase. A study is devoted to this species seeds germination, which although it’s a mandatory halophyte, it’s less tolerant to salt stress in germination’ phase. Germinative tests are carried out in laboratory, on seeds putted in Petri dishes placed at different temperatures. They enable us to determine the optimal germination temperature, which is 40&#176C while the cold one (5&#176C) completely inhibits it. The seeds of Arthrocnemum glaucum show an optimum germination of 44% in distilled water (NaCl 0 mm) similar to glycophytes but they differ in their ability to germinate at higher salinities. Beyond 300 mM germination is completely inhibited. Prior to break dormancy seeds, a cold treatment at 5&#176C has been achieved;the germination capacity of the seeds increases with the duration of the cold treatment. It increases from 30% after 2 weeks of treatment to 80% after a treatment of 27 weeks. 展开更多
关键词 Arthrocnemum glaucum Halophilic Vegetation germination salt stress Oran(Algeria)
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Plant hormone treatments to alleviate the effects of salt stress on germination of Betula platyphylla seeds 被引量:1
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作者 Zhixin Li Xiaona Pei +3 位作者 Shaopeng Yin Xianbo Lang Xiyang Zhao Guan-Zheng Qu 《Journal of Forestry Research》 SCIE CAS CSCD 2019年第3期779-787,共9页
We analyzed the germination traits (germination rate, germination potential, and germination index) of seeds from 23 half-sib families of birch (Betula platyphylla). The germination rates of the 23 families ranged fro... We analyzed the germination traits (germination rate, germination potential, and germination index) of seeds from 23 half-sib families of birch (Betula platyphylla). The germination rates of the 23 families ranged from 38.57 to 76.50%. Seeds from the eight families germinating at the highest rates were then used for germination experiments under salt stress. Germination rate, germination potential, and germination index of these eight half-sib families decreased with increasing salt concentrations. The effects of four different hormones (6-benzylaminopurine, 6BA;gibberellin, GA3;naphthalene acetic acid, NAA;and melatonin) at various concentrations on the germination of seeds from the eight half-sib birch families under salt stress were investigated. Treatments with 6BA and GA3 alleviated the effects of salt stress on seed germination, whereas treatments with NAA and melatonin aggravated the effects of salt stress. The most effective treatments for alleviating the effects of salt stress on birch seed germination were 10 mg/L 6BA and 300 mg/L GA3. This study provides practical information for screening birch families for salt tolerance at the seed germination stage, and for cultivation of birch in saline environments. 展开更多
关键词 BETULA platyphylla germination salt stress HORMONE
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Nitrogen in Relation to Photosynthetic Capacity and Accumulation of Osmoprotectant and Nutrients in Brassica Genotypes Grown Under Salt Stress 被引量:14
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作者 Manzer H Siddiqui Firoz Mohammad +2 位作者 M Nasir Khan Mohamed H Al-Whaibi Ali H A Bahkali 《Agricultural Sciences in China》 CSCD 2010年第5期671-680,共10页
Different strategies of the application of nutrients are required to overcome the adverse effects of mustard (Brassica juncea L.) in response to NaCl stress. The objective of the present study was to determine if di... Different strategies of the application of nutrients are required to overcome the adverse effects of mustard (Brassica juncea L.) in response to NaCl stress. The objective of the present study was to determine if different added levels of nitrogen (N) in growth medium could alleviate the adverse effects of salt stress on photosynthetic capacity and accumulation of osmoprotectants and nutrients. 14 days mustard seedlings of salt-sensitive (cv. Chuutki) and salt-tolerant (cv. Radha) genotypes were fed with: (i) 0 mmol L^-1 NaCl + 0 mg N kg^-1 sand (control), (ii) 90 mmol L^-1 NaCl + 30 mg N kg^-1 sand, (iii) 90 mmol L^-1 NaCl +60 mg N kg^-1 sand, (iv) 90 mmol L^-1 NaCl +90 mg N kg^-1 sand and (v) 90 mmol L^-1NaCl+ 120 mg N kg^-1 sand. Under the condition of salinity stress, N application caused a significant ameliorative effect on both genotypes with respect to growth attributes [fresh weight (FW) and dry weight (DW)] and physio-biochemical parameters [percent water content (WC), net photosynthetic rate (PN), stomatal conductance (gs), total chlorophyll (Ch1), carbonic anhydrase (CA) activity and malondialdehyde (MDA), nitrogen (N), potassium (K) and sodium (Na) contents, and K/Na ratio] and yield attributes (number of pods/plant, seeds/pod and seed yield/plant). The salt-tolerant genotype exhibited maximum value for growth, physio-biochemical and yield attributes at 60 mg N kg 1 sand than that of salt-sensitive genotype. These results suggest that application of N may ameliorate most of the attributes and prove to be a physiological remedy to increase the tolerance against the ill effects of salt stress in Brassicas. 展开更多
关键词 Brassica juncea L. carbonic anhydrase activity MALONDIALDEHYDE net photosynthetic rate osmoprotectants salt stress
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Effect of salt stress on activity of superoxide dismutase (SOD) in Ulmus pumila L. 被引量:32
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作者 SONG Fu-nan YANG Chuan-ping LIU Xue-mei LI Gong-bin 《Journal of Forestry Research》 SCIE CAS CSCD 2006年第1期13-16,共4页
房间质膜的损害忍耐和在 Ulmus pumila 叶子的血浆膜保护系统的关联词酶活动由九集中对待(0.3%,0.6%,0.9%,1.2%,1.5%,1.8%,2.1%,2.4%,3.0%)在东北林地大学的一个温室 Na_2CO_3 和 NaHCO_3mixtures 被学习,哈尔滨,中国。电解... 房间质膜的损害忍耐和在 Ulmus pumila 叶子的血浆膜保护系统的关联词酶活动由九集中对待(0.3%,0.6%,0.9%,1.2%,1.5%,1.8%,2.1%,2.4%,3.0%)在东北林地大学的一个温室 Na_2CO_3 和 NaHCO_3mixtures 被学习,哈尔滨,中国。电解质漏(REL ) 和草皮(Superoxide dismutase ) 的率在不同样品的叶子的活动被决定。结果证明在 U 的叶子的 REL。pumila 在盐集中介绍了一个慢慢地增加的趋势不到 1.5% ,它显示 U.pumila 的那房间质膜让相当强壮的抵抗有,并且在盐集中有重要增加到盐离子的损害超过 1.5% 。在 U 的叶子的草皮活动。pumila 在盐集中介绍了一个增加的趋势不到 1.5% ,幼苗的生长当盐集中超过了 1.5% 时,衰落,和发辫和叶子没有损害的症状, SODactivities 严厉地即时减少了, REL 增加了。 展开更多
关键词 盐应力 电解液 过氧化物 岐化酶 胁迫生理学
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Promotion by 5-Aminolevulinic Acid of Germination of Pakchoi (Brassica campestris ssp. chinensis var. communis Tsen et Lee) Seeds Under Salt Stress 被引量:55
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作者 Liang-Ju WANG Wei-Bing JIANG Hui LIU Wei-Qin LIU Lang KANG Xi-Lin HOU 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2005年第9期1084-1091,共8页
The seed germination and seedling growth of pakchoi (Brassica campestris ssp. chinensis var. communis Tsen et Lee cv. Hanxiao) were not significantly inhibited until the concentration of NaCl was increased to 150 mm... The seed germination and seedling growth of pakchoi (Brassica campestris ssp. chinensis var. communis Tsen et Lee cv. Hanxiao) were not significantly inhibited until the concentration of NaCl was increased to 150 mmol/L. Treatment of pakchoi seeds with exogenous 5-aminolevulinic acid (ALA), at concentrations ranging from 0.01 to 10.00 mg/L, promoted seed germination when seeds were stressed by salinity, whereas levulinic acid (LA), an inhibitor of ALA dehydrase, significantly inhibited seed germination and seedling growth, suggesting that metabolism of ALA into porphyrin compounds was necessary for seed germination and seedling growth. Determination of respiratory rate during seed germination showed that ALA increased seed respiration under both normal conditions and salt stress. Furthermore, salt stress decreased levels of endogenous ALA, as well as heme, in etiolated seedlings. More salt-tolerant cultivars of pakchoi contained higher relative levels of endogenous ALA and heme under conditions of salt stress. These results indicate that salt stress may inhibit the biosynthesis of endogenous ALA and then heme, which is necessary for seed germination, and treatment of seeds with exogenous ALA prior to germination may be associated with the biosynthesis of heme. 展开更多
关键词 5-aminolevulinic acid (ALA) germination HEME PAKCHOI respiration salt stress.
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Effect of Exogenous Melatonin on Seed Germination and Seedling Growth in Melon(Cucumis melo L.) Under Salt Stress 被引量:18
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作者 José Luis Casta?ares Carlos Alberto Bouzo 《Horticultural Plant Journal》 SCIE 2019年第2期79-87,共9页
An investigation was carried out to evaluate the effect of different application forms and concentrations of melatonin on germination and initial growth of melon plants in several different salinity conditions. We eva... An investigation was carried out to evaluate the effect of different application forms and concentrations of melatonin on germination and initial growth of melon plants in several different salinity conditions. We evaluated germination percentage and growth of seedlings and measured total chlorophyll content, relative electrolyte leakage, peroxidase activity, root viability, proline concentration, relative water content,Na^+/K^+ratio in green parts and K^+leakage from roots. Seed germination percentage under salt stress increased with treatments of 10 and50 μmol·L^(-1) melatonin. Seed treatment and plant watering with melatonin at 50 μmol·L^(-1) improved growth parameters and plants showed higher chlorophyll content as well as better biochemical parameters than non-treated plants. Results suggest that melatonin can alleviate the effect of salt stress during seed germination and first stages of plant development. 展开更多
关键词 MELON MELATONIN salt stress germination ANTIOXIDATION
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Effects of salt and alkali stress on Reaumuria soongorica germination 被引量:2
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作者 Fang Wang HongLang Xiao +1 位作者 XiaoMei Peng Shang Li 《Research in Cold and Arid Regions》 CSCD 2017年第2期158-166,共9页
Seed germination and early seedling growth are crucial stages for plant establishment. Two neutral(Na Cl and Na2SO4) and two alkali(Na HCO3 and Na2CO3) salts were selected to investigate their effects on germination a... Seed germination and early seedling growth are crucial stages for plant establishment. Two neutral(Na Cl and Na2SO4) and two alkali(Na HCO3 and Na2CO3) salts were selected to investigate their effects on germination and recovery responses in Reaumuria soongorica. Results show that both salt types significantly reduced germination and radicle elongation. The rate of germination and emergence of R. soongorica seeds continuously decreased as salinity increased, and the time to achieve maximum germination rate was delayed. The speed of seed germination dropped rapidly as salt concentration increased.Alkaline salts restricted the germination rate of R. soongorica seeds, and stresses resulting from alkaline salts and high concentrations of neutral salts resulted in many deformed seedlings. The length of the radicle and germ decreased with increasing salt concentration, but certain concentrations of salt and increased p H promoted germ growth. The results of regression analysis show that salt concentration was the dominant factor inhibiting R. soongorica seed germination rate. Salinity, buffering capacity, and p H all affected embryo growth, but salinity had the most pronounced effect. Seed viability under highly saline conditions appears to be a better indicator of adaptation to saline environments than seed germination under saline conditions. 展开更多
关键词 ALKALI stress salt stress germination RECOVERY Reaumuria soongorica
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Proteomic analysis of seed germination under salt stress in soybeans 被引量:14
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作者 Xiao-yan XU Rui FAN +2 位作者 Rui ZHENG Chun-mei LI De-yue YU 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2011年第7期507-517,共11页
Soybean (Glycine max (L.) Merrill) is a salt-sensitive crop,and its production is severely affected by saline soils.Therefore,the response of soybean seeds to salt stress during germination was investigated at both ph... Soybean (Glycine max (L.) Merrill) is a salt-sensitive crop,and its production is severely affected by saline soils.Therefore,the response of soybean seeds to salt stress during germination was investigated at both physiological and proteomic levels.The salt-tolerant cultivar Lee68 and salt-sensitive cultivar N2899 were exposed to 100 mmol/L NaCl until radicle protrusion from the seed coat.In both cultivars,the final germination percentage was not affected by salt,but the mean germination times of Lee68 and N2899 were delayed by 0.3 and 1.0 d,respectively,compared with controls.In response to salt stress,the abscisic acid content increased,and gibberellic acid (GA1+3) and isopentenyladenosine decreased.Indole-3-acetic acid increased in Lee68,but remained unchanged in N2899.The proteins extracted from germinated seeds were separated using two-dimensional gel electrophoresis (2-DE),followed by Coomassie brilliant blue G-250 staining.About 350 protein spots from 2-DE gels of pH range 3 to 10 and 650 spots from gels of pH range 4 to 7 were reproducibly resolved,of which 18 protein spots showed changes in abundance as a result of salt stress in both cultivars.After matrix-assisted laser desorption ionization-time of flight-mass spectroscopy (MALDI-TOF-MS) analysis of the differentially expressed proteins,the peptide mass fingerprint was searched against the soybean UniGene database and nine proteins were successfully identified.Ferritin and 20S proteasome subunit β-6 were up-regulated in both cultivars.Glyceraldehyde 3-phosphate dehydrogenase,glutathione S-transferase (GST) 9,GST 10,and seed maturation protein PM36 were down-regulated in Lee68 by salt,but still remained at a certain level.However,these proteins were present in lower levels in control N2899 and were up-regulated under salt stress.The results indicate that these proteins might have important roles in defense mechanisms against salt stress during soybean seed germination. 展开更多
关键词 PROTEOMICS 盐应力 种子萌芽 大豆
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Stress-Activated Protein Kinase OsSAPK7 Regulates Salt- Stress Tolerance by Modulating Diverse Stress-Defensive Responses in Rice 被引量:1
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作者 ZENG Dan WANG Chunchao +5 位作者 XIE Junpin ZHANG Fan LU Jialing SHI Xiaorong SHI Yingyao ZHOU Yongli 《Rice science》 SCIE CSCD 2021年第6期547-556,共10页
Soil salinity is an environmental threat limiting rice productivity.Identification of salinity tolerance genes and exploitation of their mechanisms in plants are vital for crop breeding.In this study,the function of s... Soil salinity is an environmental threat limiting rice productivity.Identification of salinity tolerance genes and exploitation of their mechanisms in plants are vital for crop breeding.In this study,the function of stress-activated protein kinase 7(OsSAPK7),a SnRK2 family member,was characterized in response to salt stress in rice.Compared with variety 9804,OsSAPK7-overexpression plants had a greater survival rate,increased chlorophyll and proline contents,and superoxide dismutase and catalase activities at the seedling stage under salt-stress conditio ns,as well as decreased sodium potassium ratio(Na+/K+)and malondialdehyde contents.After salt stress,the OsSAPK7 knockout plants had lower survival rates,in creased Na^K*ratios and malomdiadehyde contents,and decreased physiological parameters compared with 9804.These changes in transgenic lines suggested that OsSAPK7 increased the salt tolerance of rice by modulating ion homeostasis,redox reactions and photosynthesis.The results of RNA-Seq indicated that genes involved in redox-dependent signaling pathway,photosynthesis and zeatin synthesis pathways were significantly down-regulated in the OsSAPK7 knockout line compared with 9804 un der salt-stress conditio n,which con firmed that OsSAPK7 positively regulated salt tolera nee by modulating diverse stress-defe nsive resp on ses in rice.These findings provided novel in sights for the genetic improvement of rice and for understanding the regulatory mechanisms of salt-stress toleranee. 展开更多
关键词 RICE OsSAPK7 salt stress tolerance survival rate
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Effects of NaCl stress on seed germination, leaf gas exchange and seedling growth of Pteroceltis tatarinowii 被引量:10
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作者 FANG Sheng-zuo SONG Li-yi FU Xiang-xiang 《Journal of Forestry Research》 SCIE CAS CSCD 2006年第3期185-188,共4页
种子萌芽,叶气体交换和幼苗生长上的 NaCl 的效果被调查。P 的萌芽。tatarinowii 种子面对 NaCl 被推迟,并且当 NaCl 集中超过了 17 公里时,显著地被禁止(1.0 g · L^(-1)) 。蒸发的导致的减小评估的 NaCltreatments,有气孔的... 种子萌芽,叶气体交换和幼苗生长上的 NaCl 的效果被调查。P 的萌芽。tatarinowii 种子面对 NaCl 被推迟,并且当 NaCl 集中超过了 17 公里时,显著地被禁止(1.0 g · L^(-1)) 。蒸发的导致的减小评估的 NaCltreatments,有气孔的传导力和网光合的率和他们的减小与增加 NaCl 集中是重要的。然而,在在盐应力下面的幼苗叶子的细胞间的 CO_2 集中比控制的显著地高。在 NaCl 处理,和在根,茎,和叶子的干燥增重的重要减小也在 NaCltreatments 被遵守以后,在有增加 NaCl 集中 50 天的幸存,基础直径增长和高度增长有重要减小。生物资源增长上的有害效果在茎和叶子的情况中是更明显的。从这研究的结果建议那 P。tatarinowii 对咸度应力和咸度阀值敏感因为幼苗生长可能是更少的 34 公里(2.0 g · L ^(-1)) 。 展开更多
关键词 盐胁迫 Hoagland培养液 发芽率 净光合速率 成活率 生物量增量
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Effect of Flood Stress on Soybean Seed Germination in the Field 被引量:1
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作者 Chengjun Wu Pengyin Chen +2 位作者 Wade Hummer Ailan Zeng Mariola Klepadlo 《American Journal of Plant Sciences》 2017年第1期53-68,共16页
Flooding is an abiotic stress that impacts soybean [Glycine max (L.) Merr.] growth and reduces seed germination. Effect of flooding on soybean plant grown at different growth stage has been previously conducted and re... Flooding is an abiotic stress that impacts soybean [Glycine max (L.) Merr.] growth and reduces seed germination. Effect of flooding on soybean plant grown at different growth stage has been previously conducted and reported. However, soybean seed germination responses to flood stress are largely unknown. The objective of this study was to elucidate flooding influence on soybean seed germination after planting in the field. The research showed that seed germination rate (SGR) of each genotype, without flood stress, was significantly different and ranged between 64.7% to 84.0% and 69.0% to 90.7% while using untreated and fungicide-treated seed (P < 0.0001), respectively. Results indicated that fungicide treatment improved soybean seed survival and germination in the field. The average of SGR of high-yielding soybean group was significantly higher than those of non-high-yielding soybean (P < 0.0001). The results indicated that high-yielding trait of each genotype was correlated with seed germination and survival. Under flood stress in the field, SGR means of untreated and fungicide-treated seed significantly decreased over eight flooding treatment times (P Flooding effect on germination between untreated and fungicide-treated seed was not significantly different (P = 0.1559). Furthermore, comparing the high-yielding and flood-tolerant soybean groups showed no difference in their SGR means over eight flooding treatment times (P = 0.7687 and P = 0.8490), indicating that soybean seed germination did not depend on genotype, yield, and flood tolerance trait, and seed treated by fungicide did not increase its germination in the field under the flood stress. Hence, it is necessary to develop new soybean seed pelleting to improve seed germination in the field under flooding conditions. 展开更多
关键词 Soybean FLOOD stress Seed germination rate Fungicide APRON Maxx RTA Yield TRAIT GROUP FLOOD TRAIT GROUP
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GA3处理对盐胁迫下5种牧草种子萌发及幼苗生长的影响
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作者 崔文宁 孟静静 +4 位作者 张文香 路培 高培培 王茜 张存在 《草业科学》 CAS CSCD 北大核心 2024年第1期89-98,共10页
盐胁迫是限制种子萌发和幼苗生长的关键因子,适宜浓度的赤霉素(GA3)可有效缓解盐胁迫对种子造成的不利影响。为了解GA3对盐胁迫下牧草种子萌发和生长的缓解效应,筛选出促进盐胁迫下5种牧草种子萌发和幼苗生长的最佳GA3浓度,本研究以100 ... 盐胁迫是限制种子萌发和幼苗生长的关键因子,适宜浓度的赤霉素(GA3)可有效缓解盐胁迫对种子造成的不利影响。为了解GA3对盐胁迫下牧草种子萌发和生长的缓解效应,筛选出促进盐胁迫下5种牧草种子萌发和幼苗生长的最佳GA3浓度,本研究以100 mmol·L^(-1)的NaCl溶液模拟盐胁迫,以黑麦草(Lolium perenne)、‘朝牧1号’稗谷(Echinochloa crusgalli‘Chaomu1’)、羊草(Leymus chinensis)、紫花苜蓿(Medicago sativa)、白三叶(Trifolium repens)5种牧草种子为试材,选取不同浓度GA3进行浸种处理,测定其发芽率、发芽指数、根长、苗高、鲜重等指标。结果表明:GA3浸种可有效缓解盐胁迫对5种牧草种子萌发和幼苗生长造成的抑制作用,且不同浓度间作用差异显著(P<0.05)。150 mg·L^(-1)的GA3能有效促进黑麦草、羊草、紫花苜蓿和白三叶种子的萌发和幼苗生长;100 mg·L^(-1)的GA3能有效促进‘朝牧1号’稗谷的种子萌发,50 mg·L^(-1)的GA3能显著促进其种苗的生长。本研究结果对盐胁迫逆境下的牧草种植及土壤改良具有参考价值。 展开更多
关键词 赤霉素 盐胁迫 牧草种子 萌发 生长特性
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植物免疫诱抗剂对玉米耐盐性及产量的影响
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作者 陈敏 栾炳辉 +4 位作者 王洪涛 张伟 张晓英 刘保友 王英姿 《作物研究》 2024年第1期16-19,共4页
为探究植物免疫诱抗剂对玉米耐盐性及产量的影响,以智能聪(ZNC)为材料,于2022年在山东烟台开展试验,对盐分胁迫条件下不同浓度智能聪浸种处理的玉米种子发芽率、根系生长指标和产量进行比较分析。结果表明:在8、10 g/L NaCl溶液中,玉米... 为探究植物免疫诱抗剂对玉米耐盐性及产量的影响,以智能聪(ZNC)为材料,于2022年在山东烟台开展试验,对盐分胁迫条件下不同浓度智能聪浸种处理的玉米种子发芽率、根系生长指标和产量进行比较分析。结果表明:在8、10 g/L NaCl溶液中,玉米种子发芽率分别为27.5%、17.5%,主根长度分别为19.8、13.0 mm,显著低于其在水溶液中的发芽率(52.5%)和主根长度(55.9 mm),说明盐溶液对玉米发芽和根系生长有抑制作用。20 ng/mL智能聪浸种处理后,8、10 g/L NaCl溶液下的玉米种子发芽率分别为38.0%、25.0%,主根长度分别为24.5、15.1 mm,说明智能聪浸种能够缓解盐溶液对玉米种子发芽和根系生长的抑制作用。采用智能聪拌种结合玉米生长期兑水喷施处理,玉米产量提高5.02%。综合分析,在盐分胁迫条件下,施用智能聪可促进玉米生长,提高其产量。 展开更多
关键词 玉米 植物免疫诱抗剂 盐胁迫 发芽率 产量
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优质甜玉米骨干系在低温下种子萌发及幼苗抗性研究
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作者 廖长见 詹鹏麟 +5 位作者 张扬 卢和顶 许静 庄炜 陈山虎 林建新 《核农学报》 CAS CSCD 北大核心 2024年第7期1258-1267,共10页
甜玉米普遍存在种子发芽率低及对低温敏感的问题。为探明甜玉米种子发芽和幼苗对低温耐受性,本研究选取12份甜玉米骨干自交系,分别于25、18和12℃温度下测定萌发相关生理指标,结合相关性及聚类分析,筛选出低温萌发优势材料;选取5份优势... 甜玉米普遍存在种子发芽率低及对低温敏感的问题。为探明甜玉米种子发芽和幼苗对低温耐受性,本研究选取12份甜玉米骨干自交系,分别于25、18和12℃温度下测定萌发相关生理指标,结合相关性及聚类分析,筛选出低温萌发优势材料;选取5份优势材料开展幼苗4℃低温处理,进行耐寒相关生理指标比较及耐寒基因表达分析。结果表明,随着温度的下降,12份甜玉米骨干自交系的发芽率、发芽势、发芽指数和活力指数均受到不同程度抑制,其中发芽变幅分别为82.5、75.8和62.5个百分点;方差分析结果表明,发芽率和发芽势在温度、品种及温度与品种间互作效应均存在极显著差异;在18和12℃下,8个耐低温发芽能力单项指标表明,发芽率、发芽势、发芽指数和活力指数两两之间存在显著相关,性状聚类分析将12品种分为3类,分别为耐冷型、一般型和敏感型,筛选出闽甜系T146(1-46)、闽甜系L18(L18)、12hi307(307)、闽甜系JR8609(JR86)、闽甜系AS67(AS67)、闽甜系X9034(X90)和闽甜系AG87(AG87)为具有较好的耐低温萌发能力的优势材料。针对1-46、L18、307、JR86和AS67进行幼苗低温处理,其中L18幼苗的丙二醛(MDA)含量显著低于其余4份材料,超氧化物歧化酶(SOD)和过氧化物酶(POD)活性表现相反;抗寒基因表达分析结果发现,ZmCesA2、ZmCesA10和ZmDREB1.7在AS67和L18中表达较高,ZmMPK2在L18中表达最低;综合分析结果表明,闽甜系L18和闽甜系AS67具有较强耐低温能力。本研究结果一方面丰富了甜玉米耐寒性理论研究,另一方面为耐寒新品种选育提供了良好的材料资源。 展开更多
关键词 甜玉米 低温胁迫 发芽率 耐寒性 育种
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聚乙二醇对盐碱胁迫下花楸胚胎萌发及幼苗生理指标的影响
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作者 王晓东 赵彩鸿 +2 位作者 王瑞瑞 张悦 杨玲 《森林工程》 北大核心 2024年第4期19-28,共10页
采用室内培养皿发芽法,研究外源添加聚乙二醇、碳酸钠、碳酸氢钠对花楸胚胎萌发及幼苗生理指标的影响。结果表明,聚乙二醇处理花楸胚胎后使萌发率提高15%~25%,碳酸钠、碳酸氢钠处理使花楸胚胎的萌发率分别降低了88%和85%。聚乙二醇可缓... 采用室内培养皿发芽法,研究外源添加聚乙二醇、碳酸钠、碳酸氢钠对花楸胚胎萌发及幼苗生理指标的影响。结果表明,聚乙二醇处理花楸胚胎后使萌发率提高15%~25%,碳酸钠、碳酸氢钠处理使花楸胚胎的萌发率分别降低了88%和85%。聚乙二醇可缓解盐碱胁迫对花楸胚胎萌发的抑制,使胁迫下胚胎的萌发率提高1~2倍。聚乙二醇可提高盐碱胁迫下花楸胚胎中抗氧化酶活性,使胁迫下胚胎中超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶活性(CAT)分别提高16.45%、75%、80%。聚乙二醇使胁迫下胚胎中的丙二醛(MDA)含量降低41.44%。研究显示,聚乙二醇能够有效缓解盐碱胁迫对花楸胚胎的抑制作用,并提高花楸胚胎中的抗氧化酶活性,增强花楸的抗逆性,为建立PEG引发促进花楸胚胎萌发技术提供科学依据。 展开更多
关键词 花楸 胚胎萌发 聚乙二醇 引发 盐碱胁迫
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向日葵耐盐性差异研究及种质鉴定
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作者 杜磊 赵海红 《种子》 北大核心 2024年第1期96-101,共6页
基于12个耐盐性状,在萌发期和苗期耐盐性鉴定的基础上,综合分析了64份向日葵种质资源的耐盐特点。结果表明,盐胁迫对向日葵整个生长过程都有着明显的影响,向日葵的种子具有一定的耐盐性,并能够在一定程度上抵抗盐胁迫对种子萌发和幼苗... 基于12个耐盐性状,在萌发期和苗期耐盐性鉴定的基础上,综合分析了64份向日葵种质资源的耐盐特点。结果表明,盐胁迫对向日葵整个生长过程都有着明显的影响,向日葵的种子具有一定的耐盐性,并能够在一定程度上抵抗盐胁迫对种子萌发和幼苗生长的影响。MDA含量、脯氨酸含量和膜透性与向日葵的耐盐性呈负相关;光合速率、地下干物重、发芽势、地上干物重、株高、叶面积、出苗率、发芽率和叶绿素含量与向日葵的耐盐性呈正相关。通过因子分析,从12个性状中归纳了5个公共因子,它们的盐胁迫累积贡献率为84.058%。通过聚类分析,从64份向日葵种质资源中筛选出4份耐盐材料和7份盐敏材料。 展开更多
关键词 向日葵 萌发期 苗期 盐胁迫 因子分析
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水杨酸对盐胁迫下苦瓜种子萌发及幼苗生长的影响
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作者 马广民 《中国瓜菜》 CAS 北大核心 2024年第2期100-105,共6页
为探究水杨酸(SA)对苦瓜盐害的缓解作用,以苦瓜品种良苦1号为试材,采用拌种和叶面喷施的方法,研究不同浓度(0.001、0.01、0.1、1.0 mmol·L^(-1))SA处理对150 mmol·L^(-1) NaCl胁迫下苦瓜种子萌发和幼苗生长的影响。结果表明,0... 为探究水杨酸(SA)对苦瓜盐害的缓解作用,以苦瓜品种良苦1号为试材,采用拌种和叶面喷施的方法,研究不同浓度(0.001、0.01、0.1、1.0 mmol·L^(-1))SA处理对150 mmol·L^(-1) NaCl胁迫下苦瓜种子萌发和幼苗生长的影响。结果表明,0.01 mmol·L^(-1) SA处理显著提高了盐胁迫下苦瓜种子的发芽率、发芽势、发芽指数、活力指数及幼苗的株高、叶面积、根长、鲜质量、干质量。0.1 mmol·L^(-1)SA处理显著提高了苦瓜种子的发芽率、发芽势及幼苗的叶面积、鲜质量、干质量,对种子的发芽指数、活力指数及幼苗的株高、根长无显著影响。0.01 mmol·L^(-1) SA处理显著提高了苦瓜幼苗抗氧化酶(SOD、POD、CAT)的活性及脯氨酸、可溶性糖的含量,显著降低了丙二醛的含量。0.1 mmol·L^(-1)SA处理显著提高了苦瓜幼苗POD的活性及脯氨酸、可溶性糖的含量,对SOD、CAT的活性及丙二醛的含量无显著影响。综上,施用一定浓度外源SA对苦瓜盐害具有缓解作用,0.01 mmol·L^(-1)SA处理是缓解苦瓜盐害的最佳方案。 展开更多
关键词 苦瓜 水杨酸 盐胁迫 种子萌发 幼苗生长
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盐胁迫对台湾相思幼苗形态及相关生理指标的影响
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作者 梁文杰 《上海农业学报》 2024年第3期35-41,共7页
为探寻台湾相思种子的耐盐情况,在其种子萌发期和幼苗期分别进行不同浓度盐胁迫试验。结果表明:低浓度NaCl胁迫可提高种子发芽率,随着NaCl浓度的增高,种子发芽势、发芽率、活力指数以及发芽指数均不同程度降低,且和对照有显著差异。通... 为探寻台湾相思种子的耐盐情况,在其种子萌发期和幼苗期分别进行不同浓度盐胁迫试验。结果表明:低浓度NaCl胁迫可提高种子发芽率,随着NaCl浓度的增高,种子发芽势、发芽率、活力指数以及发芽指数均不同程度降低,且和对照有显著差异。通过拟合台湾相思发芽率和NaCl胁迫浓度回归曲线,发现种子萌发期台湾相思适宜的最大NaCl胁迫浓度为203.87 mmol∕L,NaCl致死浓度为383.16 mmol∕L;幼苗期台湾相思叶鲜重、茎鲜重、根鲜重、主根长、株高、地径以及叶片数等指标在NaCl胁迫下呈下降趋势,根冠比先上升后下降,植物的总根长、总根表面积、总根投影面积、总根体积、根平均直径、总根尖数、分枝数、交叉数均和NaCl浓度呈负相关,NaCl胁迫适宜的最大浓度为49.28 mmol∕L,台湾相思幼苗SOD、POD活力及MDA、PRO含量随着NaCl胁迫浓度的增加呈现先增高后降低的趋势。 展开更多
关键词 台湾相思 盐胁迫 种子萌发 幼苗生长 生理响应
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