[Objectives]In this experiment,wheat seeds were treated with different concentrations of gibberellin and different concentrations of salt solution to study the change of germination index of wheat seeds.[Methods]The g...[Objectives]In this experiment,wheat seeds were treated with different concentrations of gibberellin and different concentrations of salt solution to study the change of germination index of wheat seeds.[Methods]The germination rate,germination potential and germination index of wheat seeds were measured by routine methods,and the effect of exogenous gibberellin on germination of wheat seeds under salt stress was observed.[Results]The germination rate,germination potential and germination index of wheat seeds under salt stress were significantly increased after exogenous treatment of 0.25 and 0.50 g/L gibberellin within the range of salt concentration gradient.However,when the concentration of gibberellin was too high,it would inhibit the germination of seeds.[Conclusions]Appropriate concentration of gibberellin can effectively alleviate the stress caused by salt on wheat seed germination.In this experiment,the best concentration of gibberellin to alleviate salt stress was 0.25 g/L.展开更多
To explore the germination mechanism of salt-stressed rice improved by exogenous proline, and provide a theoretical basis to rice direct sowing technology for salinized soil, the effects of soaking with proline on ger...To explore the germination mechanism of salt-stressed rice improved by exogenous proline, and provide a theoretical basis to rice direct sowing technology for salinized soil, the effects of soaking with proline on germination status, amylase activity and isoenzyme were studied in this paper. The results showed that germination status including germination energy(GE), germination rate(GR), relative germination energy(RGE) and relative germination rate(RGR) significantly decreased as the same as the activities of alpha-amylase, beta-amylase and the total amylase under salt stress. Soaking with exogenous proline improved the germination status of rice under salt stress. Moreover, GE and RGE of salt-stressed rice were improved with increasing of proline concentration at the range of 5-45 mmol ·L-1. Soaking with 15 mmol ·L-1 and 30 mmol ·L-1 proline significantly improved the amylase activities(e.g. alpha-amylase, beta-amylase and total amylase) of rice under salt stress. Salt stress inhibited the express of beta-amylase isoenzyme temporarily, but had few impacts on alpha-amylase isozyme. Soaking with 30 mmol ·L-1 proline brightened District I and increased the width of 'i' brand in District II of alpha-amylase isoenzyme, but had few impacts on beta-amylase isoenzyme. In a word, soaking with proline could effectively alleviate the inhibitory effects of salt stress on seed germination.展开更多
In light of the importance of seeds germinative phase in the progress of later stages of development of any particular plant species in semi-arid and arid area, it is essential to study the germinative behavior and to...In light of the importance of seeds germinative phase in the progress of later stages of development of any particular plant species in semi-arid and arid area, it is essential to study the germinative behavior and to evaluate the tolerance of a perennial halophyte of Amaranthacaea family, Arthrocnemum glaucum (Del.) Ung. during this crucial phase. A study is devoted to this species seeds germination, which although it’s a mandatory halophyte, it’s less tolerant to salt stress in germination’ phase. Germinative tests are carried out in laboratory, on seeds putted in Petri dishes placed at different temperatures. They enable us to determine the optimal germination temperature, which is 40°C while the cold one (5°C) completely inhibits it. The seeds of Arthrocnemum glaucum show an optimum germination of 44% in distilled water (NaCl 0 mm) similar to glycophytes but they differ in their ability to germinate at higher salinities. Beyond 300 mM germination is completely inhibited. Prior to break dormancy seeds, a cold treatment at 5°C has been achieved;the germination capacity of the seeds increases with the duration of the cold treatment. It increases from 30% after 2 weeks of treatment to 80% after a treatment of 27 weeks.展开更多
We analyzed the germination traits (germination rate, germination potential, and germination index) of seeds from 23 half-sib families of birch (Betula platyphylla). The germination rates of the 23 families ranged fro...We analyzed the germination traits (germination rate, germination potential, and germination index) of seeds from 23 half-sib families of birch (Betula platyphylla). The germination rates of the 23 families ranged from 38.57 to 76.50%. Seeds from the eight families germinating at the highest rates were then used for germination experiments under salt stress. Germination rate, germination potential, and germination index of these eight half-sib families decreased with increasing salt concentrations. The effects of four different hormones (6-benzylaminopurine, 6BA;gibberellin, GA3;naphthalene acetic acid, NAA;and melatonin) at various concentrations on the germination of seeds from the eight half-sib birch families under salt stress were investigated. Treatments with 6BA and GA3 alleviated the effects of salt stress on seed germination, whereas treatments with NAA and melatonin aggravated the effects of salt stress. The most effective treatments for alleviating the effects of salt stress on birch seed germination were 10 mg/L 6BA and 300 mg/L GA3. This study provides practical information for screening birch families for salt tolerance at the seed germination stage, and for cultivation of birch in saline environments.展开更多
Different strategies of the application of nutrients are required to overcome the adverse effects of mustard (Brassica juncea L.) in response to NaCl stress. The objective of the present study was to determine if di...Different strategies of the application of nutrients are required to overcome the adverse effects of mustard (Brassica juncea L.) in response to NaCl stress. The objective of the present study was to determine if different added levels of nitrogen (N) in growth medium could alleviate the adverse effects of salt stress on photosynthetic capacity and accumulation of osmoprotectants and nutrients. 14 days mustard seedlings of salt-sensitive (cv. Chuutki) and salt-tolerant (cv. Radha) genotypes were fed with: (i) 0 mmol L^-1 NaCl + 0 mg N kg^-1 sand (control), (ii) 90 mmol L^-1 NaCl + 30 mg N kg^-1 sand, (iii) 90 mmol L^-1 NaCl +60 mg N kg^-1 sand, (iv) 90 mmol L^-1 NaCl +90 mg N kg^-1 sand and (v) 90 mmol L^-1NaCl+ 120 mg N kg^-1 sand. Under the condition of salinity stress, N application caused a significant ameliorative effect on both genotypes with respect to growth attributes [fresh weight (FW) and dry weight (DW)] and physio-biochemical parameters [percent water content (WC), net photosynthetic rate (PN), stomatal conductance (gs), total chlorophyll (Ch1), carbonic anhydrase (CA) activity and malondialdehyde (MDA), nitrogen (N), potassium (K) and sodium (Na) contents, and K/Na ratio] and yield attributes (number of pods/plant, seeds/pod and seed yield/plant). The salt-tolerant genotype exhibited maximum value for growth, physio-biochemical and yield attributes at 60 mg N kg 1 sand than that of salt-sensitive genotype. These results suggest that application of N may ameliorate most of the attributes and prove to be a physiological remedy to increase the tolerance against the ill effects of salt stress in Brassicas.展开更多
The seed germination and seedling growth of pakchoi (Brassica campestris ssp. chinensis var. communis Tsen et Lee cv. Hanxiao) were not significantly inhibited until the concentration of NaCl was increased to 150 mm...The seed germination and seedling growth of pakchoi (Brassica campestris ssp. chinensis var. communis Tsen et Lee cv. Hanxiao) were not significantly inhibited until the concentration of NaCl was increased to 150 mmol/L. Treatment of pakchoi seeds with exogenous 5-aminolevulinic acid (ALA), at concentrations ranging from 0.01 to 10.00 mg/L, promoted seed germination when seeds were stressed by salinity, whereas levulinic acid (LA), an inhibitor of ALA dehydrase, significantly inhibited seed germination and seedling growth, suggesting that metabolism of ALA into porphyrin compounds was necessary for seed germination and seedling growth. Determination of respiratory rate during seed germination showed that ALA increased seed respiration under both normal conditions and salt stress. Furthermore, salt stress decreased levels of endogenous ALA, as well as heme, in etiolated seedlings. More salt-tolerant cultivars of pakchoi contained higher relative levels of endogenous ALA and heme under conditions of salt stress. These results indicate that salt stress may inhibit the biosynthesis of endogenous ALA and then heme, which is necessary for seed germination, and treatment of seeds with exogenous ALA prior to germination may be associated with the biosynthesis of heme.展开更多
An investigation was carried out to evaluate the effect of different application forms and concentrations of melatonin on germination and initial growth of melon plants in several different salinity conditions. We eva...An investigation was carried out to evaluate the effect of different application forms and concentrations of melatonin on germination and initial growth of melon plants in several different salinity conditions. We evaluated germination percentage and growth of seedlings and measured total chlorophyll content, relative electrolyte leakage, peroxidase activity, root viability, proline concentration, relative water content,Na^+/K^+ratio in green parts and K^+leakage from roots. Seed germination percentage under salt stress increased with treatments of 10 and50 μmol·L^(-1) melatonin. Seed treatment and plant watering with melatonin at 50 μmol·L^(-1) improved growth parameters and plants showed higher chlorophyll content as well as better biochemical parameters than non-treated plants. Results suggest that melatonin can alleviate the effect of salt stress during seed germination and first stages of plant development.展开更多
Seed germination and early seedling growth are crucial stages for plant establishment. Two neutral(Na Cl and Na2SO4) and two alkali(Na HCO3 and Na2CO3) salts were selected to investigate their effects on germination a...Seed germination and early seedling growth are crucial stages for plant establishment. Two neutral(Na Cl and Na2SO4) and two alkali(Na HCO3 and Na2CO3) salts were selected to investigate their effects on germination and recovery responses in Reaumuria soongorica. Results show that both salt types significantly reduced germination and radicle elongation. The rate of germination and emergence of R. soongorica seeds continuously decreased as salinity increased, and the time to achieve maximum germination rate was delayed. The speed of seed germination dropped rapidly as salt concentration increased.Alkaline salts restricted the germination rate of R. soongorica seeds, and stresses resulting from alkaline salts and high concentrations of neutral salts resulted in many deformed seedlings. The length of the radicle and germ decreased with increasing salt concentration, but certain concentrations of salt and increased p H promoted germ growth. The results of regression analysis show that salt concentration was the dominant factor inhibiting R. soongorica seed germination rate. Salinity, buffering capacity, and p H all affected embryo growth, but salinity had the most pronounced effect. Seed viability under highly saline conditions appears to be a better indicator of adaptation to saline environments than seed germination under saline conditions.展开更多
Soybean (Glycine max (L.) Merrill) is a salt-sensitive crop,and its production is severely affected by saline soils.Therefore,the response of soybean seeds to salt stress during germination was investigated at both ph...Soybean (Glycine max (L.) Merrill) is a salt-sensitive crop,and its production is severely affected by saline soils.Therefore,the response of soybean seeds to salt stress during germination was investigated at both physiological and proteomic levels.The salt-tolerant cultivar Lee68 and salt-sensitive cultivar N2899 were exposed to 100 mmol/L NaCl until radicle protrusion from the seed coat.In both cultivars,the final germination percentage was not affected by salt,but the mean germination times of Lee68 and N2899 were delayed by 0.3 and 1.0 d,respectively,compared with controls.In response to salt stress,the abscisic acid content increased,and gibberellic acid (GA1+3) and isopentenyladenosine decreased.Indole-3-acetic acid increased in Lee68,but remained unchanged in N2899.The proteins extracted from germinated seeds were separated using two-dimensional gel electrophoresis (2-DE),followed by Coomassie brilliant blue G-250 staining.About 350 protein spots from 2-DE gels of pH range 3 to 10 and 650 spots from gels of pH range 4 to 7 were reproducibly resolved,of which 18 protein spots showed changes in abundance as a result of salt stress in both cultivars.After matrix-assisted laser desorption ionization-time of flight-mass spectroscopy (MALDI-TOF-MS) analysis of the differentially expressed proteins,the peptide mass fingerprint was searched against the soybean UniGene database and nine proteins were successfully identified.Ferritin and 20S proteasome subunit β-6 were up-regulated in both cultivars.Glyceraldehyde 3-phosphate dehydrogenase,glutathione S-transferase (GST) 9,GST 10,and seed maturation protein PM36 were down-regulated in Lee68 by salt,but still remained at a certain level.However,these proteins were present in lower levels in control N2899 and were up-regulated under salt stress.The results indicate that these proteins might have important roles in defense mechanisms against salt stress during soybean seed germination.展开更多
Soil salinity is an environmental threat limiting rice productivity.Identification of salinity tolerance genes and exploitation of their mechanisms in plants are vital for crop breeding.In this study,the function of s...Soil salinity is an environmental threat limiting rice productivity.Identification of salinity tolerance genes and exploitation of their mechanisms in plants are vital for crop breeding.In this study,the function of stress-activated protein kinase 7(OsSAPK7),a SnRK2 family member,was characterized in response to salt stress in rice.Compared with variety 9804,OsSAPK7-overexpression plants had a greater survival rate,increased chlorophyll and proline contents,and superoxide dismutase and catalase activities at the seedling stage under salt-stress conditio ns,as well as decreased sodium potassium ratio(Na+/K+)and malondialdehyde contents.After salt stress,the OsSAPK7 knockout plants had lower survival rates,in creased Na^K*ratios and malomdiadehyde contents,and decreased physiological parameters compared with 9804.These changes in transgenic lines suggested that OsSAPK7 increased the salt tolerance of rice by modulating ion homeostasis,redox reactions and photosynthesis.The results of RNA-Seq indicated that genes involved in redox-dependent signaling pathway,photosynthesis and zeatin synthesis pathways were significantly down-regulated in the OsSAPK7 knockout line compared with 9804 un der salt-stress conditio n,which con firmed that OsSAPK7 positively regulated salt tolera nee by modulating diverse stress-defe nsive resp on ses in rice.These findings provided novel in sights for the genetic improvement of rice and for understanding the regulatory mechanisms of salt-stress toleranee.展开更多
Flooding is an abiotic stress that impacts soybean [Glycine max (L.) Merr.] growth and reduces seed germination. Effect of flooding on soybean plant grown at different growth stage has been previously conducted and re...Flooding is an abiotic stress that impacts soybean [Glycine max (L.) Merr.] growth and reduces seed germination. Effect of flooding on soybean plant grown at different growth stage has been previously conducted and reported. However, soybean seed germination responses to flood stress are largely unknown. The objective of this study was to elucidate flooding influence on soybean seed germination after planting in the field. The research showed that seed germination rate (SGR) of each genotype, without flood stress, was significantly different and ranged between 64.7% to 84.0% and 69.0% to 90.7% while using untreated and fungicide-treated seed (P < 0.0001), respectively. Results indicated that fungicide treatment improved soybean seed survival and germination in the field. The average of SGR of high-yielding soybean group was significantly higher than those of non-high-yielding soybean (P < 0.0001). The results indicated that high-yielding trait of each genotype was correlated with seed germination and survival. Under flood stress in the field, SGR means of untreated and fungicide-treated seed significantly decreased over eight flooding treatment times (P Flooding effect on germination between untreated and fungicide-treated seed was not significantly different (P = 0.1559). Furthermore, comparing the high-yielding and flood-tolerant soybean groups showed no difference in their SGR means over eight flooding treatment times (P = 0.7687 and P = 0.8490), indicating that soybean seed germination did not depend on genotype, yield, and flood tolerance trait, and seed treated by fungicide did not increase its germination in the field under the flood stress. Hence, it is necessary to develop new soybean seed pelleting to improve seed germination in the field under flooding conditions.展开更多
基金Supported by School-level Training Program of Hetian Vocational Technical College(HZ-2022-10).
文摘[Objectives]In this experiment,wheat seeds were treated with different concentrations of gibberellin and different concentrations of salt solution to study the change of germination index of wheat seeds.[Methods]The germination rate,germination potential and germination index of wheat seeds were measured by routine methods,and the effect of exogenous gibberellin on germination of wheat seeds under salt stress was observed.[Results]The germination rate,germination potential and germination index of wheat seeds under salt stress were significantly increased after exogenous treatment of 0.25 and 0.50 g/L gibberellin within the range of salt concentration gradient.However,when the concentration of gibberellin was too high,it would inhibit the germination of seeds.[Conclusions]Appropriate concentration of gibberellin can effectively alleviate the stress caused by salt on wheat seed germination.In this experiment,the best concentration of gibberellin to alleviate salt stress was 0.25 g/L.
基金Supported by the Project of Twelfth Five-year Plan for Sci & Tech Research of China in Rural Areas(2011BAD35B02-01)Program of Sci & Tech Research of China(2011BAD16B11)
文摘To explore the germination mechanism of salt-stressed rice improved by exogenous proline, and provide a theoretical basis to rice direct sowing technology for salinized soil, the effects of soaking with proline on germination status, amylase activity and isoenzyme were studied in this paper. The results showed that germination status including germination energy(GE), germination rate(GR), relative germination energy(RGE) and relative germination rate(RGR) significantly decreased as the same as the activities of alpha-amylase, beta-amylase and the total amylase under salt stress. Soaking with exogenous proline improved the germination status of rice under salt stress. Moreover, GE and RGE of salt-stressed rice were improved with increasing of proline concentration at the range of 5-45 mmol ·L-1. Soaking with 15 mmol ·L-1 and 30 mmol ·L-1 proline significantly improved the amylase activities(e.g. alpha-amylase, beta-amylase and total amylase) of rice under salt stress. Salt stress inhibited the express of beta-amylase isoenzyme temporarily, but had few impacts on alpha-amylase isozyme. Soaking with 30 mmol ·L-1 proline brightened District I and increased the width of 'i' brand in District II of alpha-amylase isoenzyme, but had few impacts on beta-amylase isoenzyme. In a word, soaking with proline could effectively alleviate the inhibitory effects of salt stress on seed germination.
文摘In light of the importance of seeds germinative phase in the progress of later stages of development of any particular plant species in semi-arid and arid area, it is essential to study the germinative behavior and to evaluate the tolerance of a perennial halophyte of Amaranthacaea family, Arthrocnemum glaucum (Del.) Ung. during this crucial phase. A study is devoted to this species seeds germination, which although it’s a mandatory halophyte, it’s less tolerant to salt stress in germination’ phase. Germinative tests are carried out in laboratory, on seeds putted in Petri dishes placed at different temperatures. They enable us to determine the optimal germination temperature, which is 40°C while the cold one (5°C) completely inhibits it. The seeds of Arthrocnemum glaucum show an optimum germination of 44% in distilled water (NaCl 0 mm) similar to glycophytes but they differ in their ability to germinate at higher salinities. Beyond 300 mM germination is completely inhibited. Prior to break dormancy seeds, a cold treatment at 5°C has been achieved;the germination capacity of the seeds increases with the duration of the cold treatment. It increases from 30% after 2 weeks of treatment to 80% after a treatment of 27 weeks.
基金supported by Grants from the National Natural Science Foundation of China(No.31770712)
文摘We analyzed the germination traits (germination rate, germination potential, and germination index) of seeds from 23 half-sib families of birch (Betula platyphylla). The germination rates of the 23 families ranged from 38.57 to 76.50%. Seeds from the eight families germinating at the highest rates were then used for germination experiments under salt stress. Germination rate, germination potential, and germination index of these eight half-sib families decreased with increasing salt concentrations. The effects of four different hormones (6-benzylaminopurine, 6BA;gibberellin, GA3;naphthalene acetic acid, NAA;and melatonin) at various concentrations on the germination of seeds from the eight half-sib birch families under salt stress were investigated. Treatments with 6BA and GA3 alleviated the effects of salt stress on seed germination, whereas treatments with NAA and melatonin aggravated the effects of salt stress. The most effective treatments for alleviating the effects of salt stress on birch seed germination were 10 mg/L 6BA and 300 mg/L GA3. This study provides practical information for screening birch families for salt tolerance at the seed germination stage, and for cultivation of birch in saline environments.
文摘Different strategies of the application of nutrients are required to overcome the adverse effects of mustard (Brassica juncea L.) in response to NaCl stress. The objective of the present study was to determine if different added levels of nitrogen (N) in growth medium could alleviate the adverse effects of salt stress on photosynthetic capacity and accumulation of osmoprotectants and nutrients. 14 days mustard seedlings of salt-sensitive (cv. Chuutki) and salt-tolerant (cv. Radha) genotypes were fed with: (i) 0 mmol L^-1 NaCl + 0 mg N kg^-1 sand (control), (ii) 90 mmol L^-1 NaCl + 30 mg N kg^-1 sand, (iii) 90 mmol L^-1 NaCl +60 mg N kg^-1 sand, (iv) 90 mmol L^-1 NaCl +90 mg N kg^-1 sand and (v) 90 mmol L^-1NaCl+ 120 mg N kg^-1 sand. Under the condition of salinity stress, N application caused a significant ameliorative effect on both genotypes with respect to growth attributes [fresh weight (FW) and dry weight (DW)] and physio-biochemical parameters [percent water content (WC), net photosynthetic rate (PN), stomatal conductance (gs), total chlorophyll (Ch1), carbonic anhydrase (CA) activity and malondialdehyde (MDA), nitrogen (N), potassium (K) and sodium (Na) contents, and K/Na ratio] and yield attributes (number of pods/plant, seeds/pod and seed yield/plant). The salt-tolerant genotype exhibited maximum value for growth, physio-biochemical and yield attributes at 60 mg N kg 1 sand than that of salt-sensitive genotype. These results suggest that application of N may ameliorate most of the attributes and prove to be a physiological remedy to increase the tolerance against the ill effects of salt stress in Brassicas.
基金the Overseas Scholarship Foundation of the Chinese Education Department,国家自然科学基金
文摘The seed germination and seedling growth of pakchoi (Brassica campestris ssp. chinensis var. communis Tsen et Lee cv. Hanxiao) were not significantly inhibited until the concentration of NaCl was increased to 150 mmol/L. Treatment of pakchoi seeds with exogenous 5-aminolevulinic acid (ALA), at concentrations ranging from 0.01 to 10.00 mg/L, promoted seed germination when seeds were stressed by salinity, whereas levulinic acid (LA), an inhibitor of ALA dehydrase, significantly inhibited seed germination and seedling growth, suggesting that metabolism of ALA into porphyrin compounds was necessary for seed germination and seedling growth. Determination of respiratory rate during seed germination showed that ALA increased seed respiration under both normal conditions and salt stress. Furthermore, salt stress decreased levels of endogenous ALA, as well as heme, in etiolated seedlings. More salt-tolerant cultivars of pakchoi contained higher relative levels of endogenous ALA and heme under conditions of salt stress. These results indicate that salt stress may inhibit the biosynthesis of endogenous ALA and then heme, which is necessary for seed germination, and treatment of seeds with exogenous ALA prior to germination may be associated with the biosynthesis of heme.
文摘An investigation was carried out to evaluate the effect of different application forms and concentrations of melatonin on germination and initial growth of melon plants in several different salinity conditions. We evaluated germination percentage and growth of seedlings and measured total chlorophyll content, relative electrolyte leakage, peroxidase activity, root viability, proline concentration, relative water content,Na^+/K^+ratio in green parts and K^+leakage from roots. Seed germination percentage under salt stress increased with treatments of 10 and50 μmol·L^(-1) melatonin. Seed treatment and plant watering with melatonin at 50 μmol·L^(-1) improved growth parameters and plants showed higher chlorophyll content as well as better biochemical parameters than non-treated plants. Results suggest that melatonin can alleviate the effect of salt stress during seed germination and first stages of plant development.
基金supported by the National Natural Science Foundation of China (41401043, 91125025)
文摘Seed germination and early seedling growth are crucial stages for plant establishment. Two neutral(Na Cl and Na2SO4) and two alkali(Na HCO3 and Na2CO3) salts were selected to investigate their effects on germination and recovery responses in Reaumuria soongorica. Results show that both salt types significantly reduced germination and radicle elongation. The rate of germination and emergence of R. soongorica seeds continuously decreased as salinity increased, and the time to achieve maximum germination rate was delayed. The speed of seed germination dropped rapidly as salt concentration increased.Alkaline salts restricted the germination rate of R. soongorica seeds, and stresses resulting from alkaline salts and high concentrations of neutral salts resulted in many deformed seedlings. The length of the radicle and germ decreased with increasing salt concentration, but certain concentrations of salt and increased p H promoted germ growth. The results of regression analysis show that salt concentration was the dominant factor inhibiting R. soongorica seed germination rate. Salinity, buffering capacity, and p H all affected embryo growth, but salinity had the most pronounced effect. Seed viability under highly saline conditions appears to be a better indicator of adaptation to saline environments than seed germination under saline conditions.
基金Project supported by the National Natural Science Foundation of China (No. 30800692)the National Basic Research Program (973) of China (Nos. 2010CB125906 and 2009CB118400)the National High-Tech R & D Program (863) of China (No. 2006AA10Z1C1)
文摘Soybean (Glycine max (L.) Merrill) is a salt-sensitive crop,and its production is severely affected by saline soils.Therefore,the response of soybean seeds to salt stress during germination was investigated at both physiological and proteomic levels.The salt-tolerant cultivar Lee68 and salt-sensitive cultivar N2899 were exposed to 100 mmol/L NaCl until radicle protrusion from the seed coat.In both cultivars,the final germination percentage was not affected by salt,but the mean germination times of Lee68 and N2899 were delayed by 0.3 and 1.0 d,respectively,compared with controls.In response to salt stress,the abscisic acid content increased,and gibberellic acid (GA1+3) and isopentenyladenosine decreased.Indole-3-acetic acid increased in Lee68,but remained unchanged in N2899.The proteins extracted from germinated seeds were separated using two-dimensional gel electrophoresis (2-DE),followed by Coomassie brilliant blue G-250 staining.About 350 protein spots from 2-DE gels of pH range 3 to 10 and 650 spots from gels of pH range 4 to 7 were reproducibly resolved,of which 18 protein spots showed changes in abundance as a result of salt stress in both cultivars.After matrix-assisted laser desorption ionization-time of flight-mass spectroscopy (MALDI-TOF-MS) analysis of the differentially expressed proteins,the peptide mass fingerprint was searched against the soybean UniGene database and nine proteins were successfully identified.Ferritin and 20S proteasome subunit β-6 were up-regulated in both cultivars.Glyceraldehyde 3-phosphate dehydrogenase,glutathione S-transferase (GST) 9,GST 10,and seed maturation protein PM36 were down-regulated in Lee68 by salt,but still remained at a certain level.However,these proteins were present in lower levels in control N2899 and were up-regulated under salt stress.The results indicate that these proteins might have important roles in defense mechanisms against salt stress during soybean seed germination.
基金supported by the National Key Research and Development Program of China(Grant No.2016YFD0100101)the National High-Tech Program of China(Grant No.2014AA10A603)the Bill&Melinda Gates Foundation(Grant No.OPP1130530).
文摘Soil salinity is an environmental threat limiting rice productivity.Identification of salinity tolerance genes and exploitation of their mechanisms in plants are vital for crop breeding.In this study,the function of stress-activated protein kinase 7(OsSAPK7),a SnRK2 family member,was characterized in response to salt stress in rice.Compared with variety 9804,OsSAPK7-overexpression plants had a greater survival rate,increased chlorophyll and proline contents,and superoxide dismutase and catalase activities at the seedling stage under salt-stress conditio ns,as well as decreased sodium potassium ratio(Na+/K+)and malondialdehyde contents.After salt stress,the OsSAPK7 knockout plants had lower survival rates,in creased Na^K*ratios and malomdiadehyde contents,and decreased physiological parameters compared with 9804.These changes in transgenic lines suggested that OsSAPK7 increased the salt tolerance of rice by modulating ion homeostasis,redox reactions and photosynthesis.The results of RNA-Seq indicated that genes involved in redox-dependent signaling pathway,photosynthesis and zeatin synthesis pathways were significantly down-regulated in the OsSAPK7 knockout line compared with 9804 un der salt-stress conditio n,which con firmed that OsSAPK7 positively regulated salt tolera nee by modulating diverse stress-defe nsive resp on ses in rice.These findings provided novel in sights for the genetic improvement of rice and for understanding the regulatory mechanisms of salt-stress toleranee.
文摘Flooding is an abiotic stress that impacts soybean [Glycine max (L.) Merr.] growth and reduces seed germination. Effect of flooding on soybean plant grown at different growth stage has been previously conducted and reported. However, soybean seed germination responses to flood stress are largely unknown. The objective of this study was to elucidate flooding influence on soybean seed germination after planting in the field. The research showed that seed germination rate (SGR) of each genotype, without flood stress, was significantly different and ranged between 64.7% to 84.0% and 69.0% to 90.7% while using untreated and fungicide-treated seed (P < 0.0001), respectively. Results indicated that fungicide treatment improved soybean seed survival and germination in the field. The average of SGR of high-yielding soybean group was significantly higher than those of non-high-yielding soybean (P < 0.0001). The results indicated that high-yielding trait of each genotype was correlated with seed germination and survival. Under flood stress in the field, SGR means of untreated and fungicide-treated seed significantly decreased over eight flooding treatment times (P Flooding effect on germination between untreated and fungicide-treated seed was not significantly different (P = 0.1559). Furthermore, comparing the high-yielding and flood-tolerant soybean groups showed no difference in their SGR means over eight flooding treatment times (P = 0.7687 and P = 0.8490), indicating that soybean seed germination did not depend on genotype, yield, and flood tolerance trait, and seed treated by fungicide did not increase its germination in the field under the flood stress. Hence, it is necessary to develop new soybean seed pelleting to improve seed germination in the field under flooding conditions.