Objective:To detect and characterize Chlamydophila psittaci(C.psittaci) in asymptomatic feral pigeons in central Thailand.Methods:A total 814 swabs from the trachea and cloacae of 407non-clinical feral pigeons in cent...Objective:To detect and characterize Chlamydophila psittaci(C.psittaci) in asymptomatic feral pigeons in central Thailand.Methods:A total 814 swabs from the trachea and cloacae of 407non-clinical feral pigeons in central Thailand were collected and tested for the presence of C.psittaci.Results:A 10.8%of feral pigeons in the sample group were positive as determined by nested PCR primer specific to C.psittaci.The outer membrane protein A(orupA) gene of positive samples exhibited amino acid identity of C.psittaci ranging from 71 to 100%and were grouped in genotype B.Exceptionally,BF1676-56 isolate was closely related to Chlamydia avium with99%identification of the I6 S ribosomal(r) RNA gene.Conclusions:This is the first report on C.psittaci isolated from asymptomatic feral pigeons in Thailand,which provides knowledge for the disease status in pigeon populations in Thailand.展开更多
[Objective] This study aimed to make a survey of domestic pigeons for infection with Eimeria species of coccidia was conducted in Guangdong Province, southern China. [Method] A total of 244 fecal samples(102 from Huiz...[Objective] This study aimed to make a survey of domestic pigeons for infection with Eimeria species of coccidia was conducted in Guangdong Province, southern China. [Method] A total of 244 fecal samples(102 from Huizhou, 83 from Jiangmen, 35 from Chaozhou and 24 from Guangzhou, respectively) in four pigeon breeding farms between June 2012 and March2013 were collected and examined microscopically. [Results] Eimeria oocysts were seen in 223(91.4%) fecal samples, with three species, namely E. labbeana, E.columbae and E. kapotei. E. labbeana was the most common species in Guangdong provinces with an overall prevalence of 91.4%, while a slightly lower incidence of E. columbae and E. kapotei species was detected with11.1% and 6.6%, representatively. The prevalence in different months varied ranging from 83.3% to 91.7%, with the highest prevalence in summer. Nestling groups showed obviously high infection than adult pigeons. [Conclusion] The present survey indicated the wide and severe prevalence of Eimeria infection in Guangdong domestic pigeons, which suggested that integrated measures should be taken to control and prevent coccidiosis of pigeons in this province.展开更多
Objective;To determine the presence of zoonotic tick-borne bacteria in feral pigeons(Columba lixia domestica) from urban areas.Methods:Spleen samples from 84 feral pigeons,found dead with traumatic injuries in urban a...Objective;To determine the presence of zoonotic tick-borne bacteria in feral pigeons(Columba lixia domestica) from urban areas.Methods:Spleen samples from 84 feral pigeons,found dead with traumatic injuries in urban areas,were examined by PCR to detect DNA of Anaplasma phagocytophilum,Bartonella spp.,Borrelia burgdorferi sensu lato.Coxiella burnetii.Rickettsia spp.,and Chlamydnphila spp.Results:Twenty(23.8%) pigeons were infected by tick-borne agents,in particular 2(2.38%) animals resulted positive for Bartonella spp.,5(5.95%) for Coxiella burnetii.5(5.95%) for Rickettsia spp.,13(15.47%) for Borrelia burgdorferi sensu lato.All birds scored negative for anaplasma phagocytophilum.Moreover,17(20.23%) pigeons were positive for Chlamydophila spp.and among them 10(11.9%) for Chlamydophila psittaci,Mixed infections by two or three agents were detected in 8(9.52%) animals.Conclusions:Feral pigeons living in urban and periurban areas are a hazard for the human health as source of several pathogens.The obtained results confirm pigeons as reservoirs of chlamydial agents and suggest that they may be involved in the epidemiology of zoonotic tick-borne infections too.展开更多
This study investigated effects of post-training treatment with phaclofen, GABAB receptor antagonist, on the memory of food location and on the expression of Synapsin I in the hippocampus of pigeons. Pigeons were trai...This study investigated effects of post-training treatment with phaclofen, GABAB receptor antagonist, on the memory of food location and on the expression of Synapsin I in the hippocampus of pigeons. Pigeons were trained in food location (7 sessions) and underwent post-training treatment with phaclofen (0.3 mg/kg, i.p.;PHAC), saline (SAL) or non-treated (NTR). Testing for memory persistence occurred 7 days after the last training session (PHACR, SALR and NTRR Groups). Pigeons treated with phaclofen had lower latency and higher correct choice values than saline and non-treated controls (p < 0.05). Analysis of hippocampus tissue indicated that Synapsin I-positive cell counts were higher in pigeons treated with phaclofen than in saline and non-treated controls (p < 0.05). Data indicated enhancement of consolidation and persistence of food location memory, and up-regulation of Synapsin I expression in the hippocampus of pigeons, which were related with post-training blockade of GABAB receptors.展开更多
[ Objective]This study aimed to compare differences in the pathogenicity between genotype VI Newcastle disease virus (NDV) strains from pigeons and chickens in pigeons. [ Method] Two-month-old pigeons were artificia...[ Objective]This study aimed to compare differences in the pathogenicity between genotype VI Newcastle disease virus (NDV) strains from pigeons and chickens in pigeons. [ Method] Two-month-old pigeons were artificially inoculated with ZJ3 strain from chickens and WX-10-07-Pi strain from pigeons. After inoc- ulation, the clinical symptoms, pathological anatomical changes, tracheal and cloacal detoxification, and histological lesions of experimental pigeons were observed. [ Result] Both ZJ3 strain and WX-10-07-Pi strain could infect pigeons with the incidence rate of 100%, but the mortality rate was 0. The cloacal detoxification time of pigeons in WX-10-07-Pi infection group was longer, and the virus detection rate was higher; in addition, the virus could be detected in various tissues and organs of inoculated pigeons. [ Conclusion] Different genotypes of NDV are pathogenic to pigeons, but the pathogenicity is related to the features of NDV strains. Genotype VIb NDV from pigeons can be carried and discharged for a long term in pigeons, which can spread in pigeon groups more easily.展开更多
To explore the effects of breeds,number of nurturing squabs,and temperature on nest feed conversion ratio and analyze the effects of number of nurturing squabs on parent pigeon body mass,fertility rate,and laying inte...To explore the effects of breeds,number of nurturing squabs,and temperature on nest feed conversion ratio and analyze the effects of number of nurturing squabs on parent pigeon body mass,fertility rate,and laying intervals,5 widely used breeds of pigeon were nurtured under the condition of natural laying and hatching in this research.In each breed,24 pair pigeons nurturing 1,2,3,and 4 squabs were chosen to measure the production performance,respectively.The results indicated that different numbers of nurturing squabs had a significant effect on parent pigeon body mass changes and survival rate of 28-d old squabs(P<0.05);the nest feed conversion ratio of breeds nurturing 1 squab(7.58)was extremely significantly higher than those of nurturing 2,3,and 4 squabs(P<0.01);the nest feed conversion ratio of breeds nurturing 2 squabs(4.75)was extremely significantly higher than those of nurturing 3(3.95)and 4 squabs(3.75)(P<0.01);there was no significant difference between breeds nurturing 3 and 4 squabs(P>0.05).Laying intervals of breeds nurturing 4 squabs(43.8 d)were significantly higher than those of nurturing 3(37.4 d),2(35.4 d),and 1(33.8 d)squabs(P<0.05).Nest feed conversion ratio significantly increased with the decrease of environment temperature(P<0.05).The breed showed no effect on the nest feed conversion ratio.In production,nurturing 3 squabs of parent pigeons is appropriate.In some cold areas in winter,cold protection measures should be taken.展开更多
Pigeon peas, a type of pulse, have immense nutritional potential to improve health in arid and semi-arid regions. However, unlocking this potential relies heavily on understanding their technological properties, such ...Pigeon peas, a type of pulse, have immense nutritional potential to improve health in arid and semi-arid regions. However, unlocking this potential relies heavily on understanding their technological properties, such as hydration rate, volumetric expansion, and cooking time. These properties directly influence processing, accessibility, and acceptability as a food source. However, there is limited information on technological properties of improved varieties. The study aimed to determine technological properties of improved pigeon pea varieties grown in Machakos County. Seven improved pigeon peas varieties namely: KARI Mbaazi 1, KARI Mbaazi 2, ICEAP 00850, KAT 60/8, Mituki, Egerton Mbaazi 1, Egerton Mbaazi 2 and ICEAP 00554 (control variety) were used in this study. These varieties were tested for water absorption capacity (WAC), volumetric expansion, density, cooking time (CT) and total soluble solids (TSS) in the broth. The experiment was arranged in a Completely Randomized Design (CRD) replicated three times. Data analysis was conducted using SAS software version 9.1.3 (SAS, 2006). Means separation was done using Tukey’s honestly significant difference (HSD) at 95% Confidence Level. There were significant differences in water absorption capacity (WAC), volumetric expansion, density, TSS, and CT among the improved varieties (p p < 0.05). KARI Mbaazi 2 exhibited the greatest volumetric expansion after cooking (VEAC) at 11%. Additionally, control variety recorded the highest water absorption capacity (125.48%), which was significantly greater compared to the improved pigeon pea varieties. Cooking time in minutes was shortest for Mituki (46.0) and KAT 60/8 (55.7) and longest for both KARI Mbaazi 1 and ICEAP00850 at 160 minutes. All the varieties showed high TSS ranging from 10.5 to 26.7% indicating the potential to select varieties with desired flavour profiles. Improved pigeon pea varieties (Mituki and KAT60/8) displayed desired technological properties alongside the control variety. These findings inform the specific culinary applications and nutritional needs which enhance utilisation of pigeon peas as food. Further research is needed to determine the impact of the technological properties on the digestibility and glycaemic index of pigeon peas.展开更多
[Objective]This study aimed to explore the inheritance pattem of feather color in hybrid offspring of Texan pigeon and American Silver King pigeon, so as to provide a theoretical basis for the breeding of feather colo...[Objective]This study aimed to explore the inheritance pattem of feather color in hybrid offspring of Texan pigeon and American Silver King pigeon, so as to provide a theoretical basis for the breeding of feather color auto-sexing strains. [Method]The segregation situation of feather color in the male and female offspring of Texan pigeon (♂)×American Silver King pigeon (♀)(TKYW) and American Silver King pigeon (♂) ×Texan pigeon (♀) (YWTK) was analyzed. [Result]The feather color of TKYW offspring was all slate gray. Among the offspring of YWTK, the feather color of the males was all slate gray, while the feather color of the females was all silver gray; and the ratio between different colors was 1:1 (P 〉 0.05). The F1 generation of YWTK was subjected to selfing, and the feather color of the male and female F2 generation was analyzed. It was found that the ratio of silver gray (male) to slate gray (male) to silver gray (female) to slate gray (female) was 1:1:1:1 (P 〉 0.05). On the basis of the above results, it could be preliminarily predicated that the feather color control genes of Texan pigeon are Z^BZB and Z^BW and those of American Silver King pigeon are Z^bZ^b and Z^bW. There is a cumulative effect on B which is dominant for b. [Conclusion] This study will better solve the problem of male and female identification of pigeon in the early period.展开更多
Glucose-6-phosphate dehydrogenase has been purified from pigeon pea (Cajanus cajan) seeds and subjected to characterization. The enzyme was purified 123.69 fold with a yield of 21.37% by ammonium sulphate fractionatio...Glucose-6-phosphate dehydrogenase has been purified from pigeon pea (Cajanus cajan) seeds and subjected to characterization. The enzyme was purified 123.69 fold with a yield of 21.37% by ammonium sulphate fractionation, PEG-4000 precipitation, CM cellulose column chromatography and DEAE cellulose column chromatography. The catalytically active enzyme is a dimer of 113 KDa with a subunit molecular weight of 55 KDa. Thermal inactivation of enzyme follows first order kinetics at 30°C and 40°C with half life of 6 and 1.5 min respectively. Km value for glucose-6-phosphate and NADP+ was found to be 2.68 mM and 0.75 mM respectively whereas Vmax value was found to be 0.11 U/mL and 0.13 U/mL respectively. The enzyme shows more affinity towards NADP+ than glucose-6-phosphate. The pKa value was found to be 10.41 indicating that the amino acid residue at active site might be lysine. The enzyme exhibited maximum catalytic activity at pH 8.2. The enzyme was found to be highly thermosensitive with gradual loss of activity above 30°C temperature.展开更多
Isolates of Newcastle disease virus (NDV) from deceased wild and domestic pigeons in Kazakhstan were obtained from the Almaty region during 2005 and were genotypically analyzed by using reverse transcription polymeras...Isolates of Newcastle disease virus (NDV) from deceased wild and domestic pigeons in Kazakhstan were obtained from the Almaty region during 2005 and were genotypically analyzed by using reverse transcription polymerase chain reaction (RT-PCR) with primers specific to the viral fusion (F) protein gene. Part of the amplified F protein DNA product (nucleotide sequence 47-422) and the deduced amino acid sequences were compared phylogenetically with those from strains previously reported in other geographic regions. Phylogenetic analysis indicated that the Kazakhstanian pigeon paramyxovirus type 1 (PPMV-1) isolates belong to genotype VI or 4bii. To our knowledge, this is the first reported VI isolates that possess the sequences of 112 GKRQKR116* F117 within the F0 protein. The information is fundamental to improving the efficiency of control strategies and vaccine development for NDV.展开更多
The study was carried out on the impact assessment of the form and nature of the different nitrogen sources used in culture on growth and nodulation of pigeon pea (Cajanus cajan (L.) Millsp) during 3 months of observa...The study was carried out on the impact assessment of the form and nature of the different nitrogen sources used in culture on growth and nodulation of pigeon pea (Cajanus cajan (L.) Millsp) during 3 months of observation on sandy soil of Mount Amba (at the University of Kinshasa in the Democratic Republic of Congo). Height and collar diameter growth, underground and total biomass dry weight, number and dry pea nodules and nodulation index have been evaluated at 4, 6, 8, 10 and 12 weeks of growth to assess the response of the legume to mineral nitrogen and organic nitrogen from chickens manure and pig feces. After observations, it appears that all the treatments soil showed different effects on growth and nodulation of pigeon pea. Therefore, only the soil received 1 kg of pig feces stimulated (p ≤ 0.05) the development of pigeon pea which resulted in good growth (nodulation 116.8 nodules and nodulation index of 5.0) and biomass production (25.3 gr per plant) while the addition of chicken manure, whatever the dose, reduced them.展开更多
[Objective] The paper was to provide a basis for scientific prevention and control of pigeon Newcastle disease(ND).[Method] The HN gene of eight pigeon NDV strains isolated from different pigeon farms in Guangxi wer...[Objective] The paper was to provide a basis for scientific prevention and control of pigeon Newcastle disease(ND).[Method] The HN gene of eight pigeon NDV strains isolated from different pigeon farms in Guangxi were amplified by RT-PCR,sequenced and analyzed.The molecular evolution characteristics of HN gene of pigeon NDV isolates in Guangxi was discussed.[Result] The nucleotide sequence length of HN gene of the eight NDV isolates was 1 716 bp,encoding 571 amino acids.They belonged to virulent group C,and the gene length characteristic of HN gene accorded with virulent strain.Analysis of nucleotide homologies indicated that the eight NDV isolates shared higher homology with genotype VIb,ranging from 90.4% to 99.5%.Phylogenetic tree analysis demonstrated that the genetic relationship between the eight NDV strains in Gangxi and the NDV isolates from Guangxi,Guangdong,Jilin,Liaoning,Yunnan and Heilongjiang during 2011 and 2013 was close.They were located in the same cladogram branch.[Conclusion] We assume that the eight pigeon NDV isolates in Guangxi all belong to the gene class II genotype VI b NDV.展开更多
Today, the green pigeon (Treron calvus, TEMMINCK, 1811) holds an in-creasingly important place in the dietary habits of the populations of south-east of C?te d’Ivoire, especially those of Grand-Bassam. To provide foo...Today, the green pigeon (Treron calvus, TEMMINCK, 1811) holds an in-creasingly important place in the dietary habits of the populations of south-east of C?te d’Ivoire, especially those of Grand-Bassam. To provide food security to these customers a study was carried out. Objective: The current study is to identify the different protozoa and fungus that could po-tentially infect the green pigeon’s gut. Materials and Methods: The study was carried out on protozoa and digestive fungus in this species of pigeon in the south-east of C?te d’Ivoire. It has been carried out from November 2020 to February 2021. A total of sixty freshly dead pigeons, including 10 males and 50 females, have been bought in Grand-Bassam. After autopsy, the gut of each animal was preserved in formalin (10%) for a subsequent histopatho-logical examination. Results: The analysis of the results revealed the fol-lowing pathogen genus: Cryptosporidium spp, Histomonas spp, Toxoplasma gondii and Trichomonas spp for protozoa. Cryptococcus neoformans for fungus. The calculation of prevalence gave the following values: 63% [20;40] for protozoa and 37% [10;40] for fungus. Conclusion: The study showed that the intestine of the green pigeon of the Grand-Bassam region is infested by a range of pathogens. Namely: Cryptosporidium spp, Histomonas spp, Toxoplasma gondii, Trichonomas spp and Cryptococcus neoformans. Cryptococcus neoformans was the most common pathogen encountered in this study. Among these pathogens, some like Cryptococcus neoformans and Toxoplasma gondii are zoonosis. Thus, the present study deserves further investigation to know more about the nature of human infection in C?te d’Ivoire. Furthermore, given the diversity of digestive pathogens including zoonosis one, a reasonable recommendation to consumers would be stop eating the intestines.展开更多
Aims: The aims of this work undertaken to investigate and identify the prevalence of Enterococcus spp isolated from Chicken and pigeon meat and determine the using antibiotics sensitivity against isolates for human sa...Aims: The aims of this work undertaken to investigate and identify the prevalence of Enterococcus spp isolated from Chicken and pigeon meat and determine the using antibiotics sensitivity against isolates for human safety. This study was conducted in center of excellence lab in Microbiology Department of Primeasia University, Banani, Dhaka-1213, Bangladesh during August, 2017 to March, 2018. Methodology and Results: Twenty four samples were collected in pre-sterilized zip-lock bags from different Gazipur area in different local meat vendors. All samples were transported to the Microbiology Laboratory of Primeasia University and analyzed according to standard analysis methods. Total viable count (TVC) and Total Enterocooci count (TEC) were estimated by using plate count agar (PCA) and Bile Esculineagar plates respectively. Various Selective media and Biochemical tests were used for presumptive isolation and identification. Kirby-Bauer disc diffusion method on Mueller Hinton agar media was used for the determination of sensitivity of the isolated strains to commonly prescribe antibiotics. A total of 24 meat samples were examined of which 92% were positive to Enterococcus spp whereas 62.5% were Chicken and 32.5% pigeon. The counts of enterococci range of 0 - 6.49 log CFU/gm in chicken and 4.4 - 6.4 log CFU/gm in pigeon. 22 isolates of enterococci, 77.27% were resistance to Azithromycin, 54.55% to Nalidixic acid and 50% to Sulfamethoxazole. We found high sensitivity of antimicrobial agent against to Imipenem (100%), Ciprofloxacin (81.81%) and (77.27%) both Tetracycline and Erythromycin. Conclusion: Significance and Impact of the study: The study result expressed that awareness of the meat pathogen and when consumption of meat was well cooked of our daily life.展开更多
This study was aimed to determine the level of selected metals and nutritional composition of pigeon pea seed collected from seven districts of Wolaita zone. <span>A</span><span> </span><spa...This study was aimed to determine the level of selected metals and nutritional composition of pigeon pea seed collected from seven districts of Wolaita zone. <span>A</span><span> </span><span>wet digestion procedure involving the use of mixtures of (69%</span><span> </span><span>-</span><span> </span><span>72%) HNO</span><sub><span style="vertical-align:sub;">3</span></sub><span> and (70%) HClO</span><sub><span style="vertical-align:sub;">4</span></sub><span> at an optimum temperature and time duration w</span><span>as</span><span> used to determine metals by using flame atomic absorption spectrometry. Kjeldahl digestion method, Soxhlet extraction and furnace were used to determine nutritional values of pigeon pea, and physicochemical properties of soils were assessed using standard methods. The results showed that the levels of concentration of metals in mg/kg dry weight were ranged 105.17 to 144.07 for K, 8.95 to 12.67 for Mg, 7.74 to 12.27 for Ca, 0.247 to 0.543 for Fe, 0.122 to 0.313 for Zn, 0.061 to 0.432 for Mn, 0.087 to 0.134 for Cu and 0.0011 to 0.00196 for Cr. The proximate composition of pigeon pea was in the range of 19.28% to 25.79% for crude protein, 0.993% to 1.75% for crude fat, 3.75% to 5.31% for ash, 10.65% to 13.73% for moisture, 2.28% to 3.06% for fiber, 54.36% to 60.1% for carbohydrate and 326.8 to 345.23 Kcal for energy. The pH of the soil was in the range from pH 5.09 (strongly acidic) to 6.77 (slightly acidic), EC of the soil ranged from 0.047 to 0.14 dS/m (low)</span><span>,</span><span> the soil OC level was from 1.6% to 2.42% (moderate), total Nitrogen was from 0.12% to 0.23% (low to moderate), the available Phosphorus content of the soil </span><span>wa</span><span>s from 6.82 to 13.52 mg/kg (low to moderate), CEC value of the soil </span><span>wa</span><span>s from 14.8 to 23.53 meq/100g (moderate). The textural classes of soil were sandy clay loam for all sites except Abela abaya. The study confirmed that pigeon pea </span><span>wa</span><span>s a good source of proteins, carbohydrates, and selected metals such as Mg, K, Ca, Fe, Zn, Mn, Cu, and Cr. The concentration of metals and nutritional compositions of pigeon pea seed were found at a permissible level.</span>展开更多
Foraging ecology of the globally endangered Nilgiri Wood Pigeon(Columba elphinstonii) was investigated in the Western Ghats,India,using faecal sampling and direct observations.The birds fed on fruits of 39 plant speci...Foraging ecology of the globally endangered Nilgiri Wood Pigeon(Columba elphinstonii) was investigated in the Western Ghats,India,using faecal sampling and direct observations.The birds fed on fruits of 39 plant species,on seeds of 11 species,flowers and leaf buds of four species and a few ground invertebrates.Direct observations across seasons showed nine plant species were selected more often(47% of observations),with fruits of the members of the family Lauraceae the most preferred.Gleaning accounted for 76% of feeding methods throughout the year.Feeding was mainly from twigs at the edges on the upper and middle canopy(> 6 m).Frequency of feeding on fruits correlated significantly with fruit abundance,which depended on rainfall.The present study indicates that the pigeon requires a wide variety of diets from different forest environments.Conservation of this species depends on preserving all habitat types required to meet their resource needs throughout the year.展开更多
试验旨在探究“2+2”生产模式下不同粗蛋白质水平对白王种鸽繁殖性能和乳鸽生长性能的影响。试验选取产蛋性能相近的300日龄健康种鸽168对,随机分为4组,每组7个重复,每个重复6对种鸽,每对种鸽哺育2只乳鸽。试验Ⅰ组、Ⅱ组、Ⅲ组和Ⅳ组...试验旨在探究“2+2”生产模式下不同粗蛋白质水平对白王种鸽繁殖性能和乳鸽生长性能的影响。试验选取产蛋性能相近的300日龄健康种鸽168对,随机分为4组,每组7个重复,每个重复6对种鸽,每对种鸽哺育2只乳鸽。试验Ⅰ组、Ⅱ组、Ⅲ组和Ⅳ组分别饲喂粗蛋白质水平为13.43%、15.04%、16.64%和18.25%的饲粮。试验期46 d,其中孵化期18 d (1~18 d),哺乳期28 d (19~46 d)。结果显示,试验Ⅱ组、Ⅲ组产蛋间隔显著低于试验Ⅰ组(P<0.05),试验Ⅲ组种蛋受精率显著高于试验Ⅰ组、Ⅳ组(P<0.05),试验Ⅲ组入孵蛋孵化率极显著高于试验Ⅰ组、Ⅱ组、Ⅳ组(P<0.01)。试验Ⅲ组乳鸽28日龄体重显著高于试验Ⅰ组(P<0.05),乳鸽屠宰率极显著高于试验Ⅰ组(P<0.01),乳鸽胸肌率显著高于试验Ⅰ组、Ⅱ组、Ⅳ组(P<0.05)。试验Ⅱ组、Ⅲ组、Ⅳ组窝料重比1极显著低于试验Ⅰ组(P<0.01)。研究表明,不同粗蛋白质水平影响了种鸽的繁殖性能、乳鸽的生长性能以及窝料重比,在“2+2”生产模式下,建议白王种鸽饲粮适宜粗蛋白质水平为16.64%。展开更多
基金financially supported by the Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals.Faculty of Veterinary Science.Mahidol University
文摘Objective:To detect and characterize Chlamydophila psittaci(C.psittaci) in asymptomatic feral pigeons in central Thailand.Methods:A total 814 swabs from the trachea and cloacae of 407non-clinical feral pigeons in central Thailand were collected and tested for the presence of C.psittaci.Results:A 10.8%of feral pigeons in the sample group were positive as determined by nested PCR primer specific to C.psittaci.The outer membrane protein A(orupA) gene of positive samples exhibited amino acid identity of C.psittaci ranging from 71 to 100%and were grouped in genotype B.Exceptionally,BF1676-56 isolate was closely related to Chlamydia avium with99%identification of the I6 S ribosomal(r) RNA gene.Conclusions:This is the first report on C.psittaci isolated from asymptomatic feral pigeons in Thailand,which provides knowledge for the disease status in pigeon populations in Thailand.
基金Support by the National Natural Science Foundation of China(Grant No.31402186 and 31302086)the Pearl River Science and Technology New Star Project(Grant No.2012J2200059 and 2014J2200096)+1 种基金the Dean Foundation of Guangdong Academy of Agricultural Sciences(Grant No.201413)the Science and Technology Plan Projects of Guangdong Province(Grant No.2015A020209075,2013B031500005,2014B070706011 and 2013B060400037)
文摘[Objective] This study aimed to make a survey of domestic pigeons for infection with Eimeria species of coccidia was conducted in Guangdong Province, southern China. [Method] A total of 244 fecal samples(102 from Huizhou, 83 from Jiangmen, 35 from Chaozhou and 24 from Guangzhou, respectively) in four pigeon breeding farms between June 2012 and March2013 were collected and examined microscopically. [Results] Eimeria oocysts were seen in 223(91.4%) fecal samples, with three species, namely E. labbeana, E.columbae and E. kapotei. E. labbeana was the most common species in Guangdong provinces with an overall prevalence of 91.4%, while a slightly lower incidence of E. columbae and E. kapotei species was detected with11.1% and 6.6%, representatively. The prevalence in different months varied ranging from 83.3% to 91.7%, with the highest prevalence in summer. Nestling groups showed obviously high infection than adult pigeons. [Conclusion] The present survey indicated the wide and severe prevalence of Eimeria infection in Guangdong domestic pigeons, which suggested that integrated measures should be taken to control and prevent coccidiosis of pigeons in this province.
文摘Objective;To determine the presence of zoonotic tick-borne bacteria in feral pigeons(Columba lixia domestica) from urban areas.Methods:Spleen samples from 84 feral pigeons,found dead with traumatic injuries in urban areas,were examined by PCR to detect DNA of Anaplasma phagocytophilum,Bartonella spp.,Borrelia burgdorferi sensu lato.Coxiella burnetii.Rickettsia spp.,and Chlamydnphila spp.Results:Twenty(23.8%) pigeons were infected by tick-borne agents,in particular 2(2.38%) animals resulted positive for Bartonella spp.,5(5.95%) for Coxiella burnetii.5(5.95%) for Rickettsia spp.,13(15.47%) for Borrelia burgdorferi sensu lato.All birds scored negative for anaplasma phagocytophilum.Moreover,17(20.23%) pigeons were positive for Chlamydophila spp.and among them 10(11.9%) for Chlamydophila psittaci,Mixed infections by two or three agents were detected in 8(9.52%) animals.Conclusions:Feral pigeons living in urban and periurban areas are a hazard for the human health as source of several pathogens.The obtained results confirm pigeons as reservoirs of chlamydial agents and suggest that they may be involved in the epidemiology of zoonotic tick-borne infections too.
文摘This study investigated effects of post-training treatment with phaclofen, GABAB receptor antagonist, on the memory of food location and on the expression of Synapsin I in the hippocampus of pigeons. Pigeons were trained in food location (7 sessions) and underwent post-training treatment with phaclofen (0.3 mg/kg, i.p.;PHAC), saline (SAL) or non-treated (NTR). Testing for memory persistence occurred 7 days after the last training session (PHACR, SALR and NTRR Groups). Pigeons treated with phaclofen had lower latency and higher correct choice values than saline and non-treated controls (p < 0.05). Analysis of hippocampus tissue indicated that Synapsin I-positive cell counts were higher in pigeons treated with phaclofen than in saline and non-treated controls (p < 0.05). Data indicated enhancement of consolidation and persistence of food location memory, and up-regulation of Synapsin I expression in the hippocampus of pigeons, which were related with post-training blockade of GABAB receptors.
基金Supported by National Natural Science Foundation of China(31440083)Science and Technology Support Program of Jiangsu Province(BE2012366)Science and Technology Program of Jiangsu Agri-animal Husbandry Vocational College(YB201006)
文摘[ Objective]This study aimed to compare differences in the pathogenicity between genotype VI Newcastle disease virus (NDV) strains from pigeons and chickens in pigeons. [ Method] Two-month-old pigeons were artificially inoculated with ZJ3 strain from chickens and WX-10-07-Pi strain from pigeons. After inoc- ulation, the clinical symptoms, pathological anatomical changes, tracheal and cloacal detoxification, and histological lesions of experimental pigeons were observed. [ Result] Both ZJ3 strain and WX-10-07-Pi strain could infect pigeons with the incidence rate of 100%, but the mortality rate was 0. The cloacal detoxification time of pigeons in WX-10-07-Pi infection group was longer, and the virus detection rate was higher; in addition, the virus could be detected in various tissues and organs of inoculated pigeons. [ Conclusion] Different genotypes of NDV are pathogenic to pigeons, but the pathogenicity is related to the features of NDV strains. Genotype VIb NDV from pigeons can be carried and discharged for a long term in pigeons, which can spread in pigeon groups more easily.
基金Supported by Key Research and Development Program (Modern Agriculture) of Jiangsu Province(BE2017348)Modern Agriculture Project of Yangzhou City(YZ2018043)
文摘To explore the effects of breeds,number of nurturing squabs,and temperature on nest feed conversion ratio and analyze the effects of number of nurturing squabs on parent pigeon body mass,fertility rate,and laying intervals,5 widely used breeds of pigeon were nurtured under the condition of natural laying and hatching in this research.In each breed,24 pair pigeons nurturing 1,2,3,and 4 squabs were chosen to measure the production performance,respectively.The results indicated that different numbers of nurturing squabs had a significant effect on parent pigeon body mass changes and survival rate of 28-d old squabs(P<0.05);the nest feed conversion ratio of breeds nurturing 1 squab(7.58)was extremely significantly higher than those of nurturing 2,3,and 4 squabs(P<0.01);the nest feed conversion ratio of breeds nurturing 2 squabs(4.75)was extremely significantly higher than those of nurturing 3(3.95)and 4 squabs(3.75)(P<0.01);there was no significant difference between breeds nurturing 3 and 4 squabs(P>0.05).Laying intervals of breeds nurturing 4 squabs(43.8 d)were significantly higher than those of nurturing 3(37.4 d),2(35.4 d),and 1(33.8 d)squabs(P<0.05).Nest feed conversion ratio significantly increased with the decrease of environment temperature(P<0.05).The breed showed no effect on the nest feed conversion ratio.In production,nurturing 3 squabs of parent pigeons is appropriate.In some cold areas in winter,cold protection measures should be taken.
文摘Pigeon peas, a type of pulse, have immense nutritional potential to improve health in arid and semi-arid regions. However, unlocking this potential relies heavily on understanding their technological properties, such as hydration rate, volumetric expansion, and cooking time. These properties directly influence processing, accessibility, and acceptability as a food source. However, there is limited information on technological properties of improved varieties. The study aimed to determine technological properties of improved pigeon pea varieties grown in Machakos County. Seven improved pigeon peas varieties namely: KARI Mbaazi 1, KARI Mbaazi 2, ICEAP 00850, KAT 60/8, Mituki, Egerton Mbaazi 1, Egerton Mbaazi 2 and ICEAP 00554 (control variety) were used in this study. These varieties were tested for water absorption capacity (WAC), volumetric expansion, density, cooking time (CT) and total soluble solids (TSS) in the broth. The experiment was arranged in a Completely Randomized Design (CRD) replicated three times. Data analysis was conducted using SAS software version 9.1.3 (SAS, 2006). Means separation was done using Tukey’s honestly significant difference (HSD) at 95% Confidence Level. There were significant differences in water absorption capacity (WAC), volumetric expansion, density, TSS, and CT among the improved varieties (p p < 0.05). KARI Mbaazi 2 exhibited the greatest volumetric expansion after cooking (VEAC) at 11%. Additionally, control variety recorded the highest water absorption capacity (125.48%), which was significantly greater compared to the improved pigeon pea varieties. Cooking time in minutes was shortest for Mituki (46.0) and KAT 60/8 (55.7) and longest for both KARI Mbaazi 1 and ICEAP00850 at 160 minutes. All the varieties showed high TSS ranging from 10.5 to 26.7% indicating the potential to select varieties with desired flavour profiles. Improved pigeon pea varieties (Mituki and KAT60/8) displayed desired technological properties alongside the control variety. These findings inform the specific culinary applications and nutritional needs which enhance utilisation of pigeon peas as food. Further research is needed to determine the impact of the technological properties on the digestibility and glycaemic index of pigeon peas.
基金Supported by Key Research and Development Program of Jiangsu Province(Modern Agriculture)(BE2017348)~~
文摘[Objective]This study aimed to explore the inheritance pattem of feather color in hybrid offspring of Texan pigeon and American Silver King pigeon, so as to provide a theoretical basis for the breeding of feather color auto-sexing strains. [Method]The segregation situation of feather color in the male and female offspring of Texan pigeon (♂)×American Silver King pigeon (♀)(TKYW) and American Silver King pigeon (♂) ×Texan pigeon (♀) (YWTK) was analyzed. [Result]The feather color of TKYW offspring was all slate gray. Among the offspring of YWTK, the feather color of the males was all slate gray, while the feather color of the females was all silver gray; and the ratio between different colors was 1:1 (P 〉 0.05). The F1 generation of YWTK was subjected to selfing, and the feather color of the male and female F2 generation was analyzed. It was found that the ratio of silver gray (male) to slate gray (male) to silver gray (female) to slate gray (female) was 1:1:1:1 (P 〉 0.05). On the basis of the above results, it could be preliminarily predicated that the feather color control genes of Texan pigeon are Z^BZB and Z^BW and those of American Silver King pigeon are Z^bZ^b and Z^bW. There is a cumulative effect on B which is dominant for b. [Conclusion] This study will better solve the problem of male and female identification of pigeon in the early period.
文摘Glucose-6-phosphate dehydrogenase has been purified from pigeon pea (Cajanus cajan) seeds and subjected to characterization. The enzyme was purified 123.69 fold with a yield of 21.37% by ammonium sulphate fractionation, PEG-4000 precipitation, CM cellulose column chromatography and DEAE cellulose column chromatography. The catalytically active enzyme is a dimer of 113 KDa with a subunit molecular weight of 55 KDa. Thermal inactivation of enzyme follows first order kinetics at 30°C and 40°C with half life of 6 and 1.5 min respectively. Km value for glucose-6-phosphate and NADP+ was found to be 2.68 mM and 0.75 mM respectively whereas Vmax value was found to be 0.11 U/mL and 0.13 U/mL respectively. The enzyme shows more affinity towards NADP+ than glucose-6-phosphate. The pKa value was found to be 10.41 indicating that the amino acid residue at active site might be lysine. The enzyme exhibited maximum catalytic activity at pH 8.2. The enzyme was found to be highly thermosensitive with gradual loss of activity above 30°C temperature.
基金USDA-ISTC partner project(K-747p,Institute of Microbiology and Virology funding,and USDA CRIS(6612-32000-038-00D)
文摘Isolates of Newcastle disease virus (NDV) from deceased wild and domestic pigeons in Kazakhstan were obtained from the Almaty region during 2005 and were genotypically analyzed by using reverse transcription polymerase chain reaction (RT-PCR) with primers specific to the viral fusion (F) protein gene. Part of the amplified F protein DNA product (nucleotide sequence 47-422) and the deduced amino acid sequences were compared phylogenetically with those from strains previously reported in other geographic regions. Phylogenetic analysis indicated that the Kazakhstanian pigeon paramyxovirus type 1 (PPMV-1) isolates belong to genotype VI or 4bii. To our knowledge, this is the first reported VI isolates that possess the sequences of 112 GKRQKR116* F117 within the F0 protein. The information is fundamental to improving the efficiency of control strategies and vaccine development for NDV.
文摘The study was carried out on the impact assessment of the form and nature of the different nitrogen sources used in culture on growth and nodulation of pigeon pea (Cajanus cajan (L.) Millsp) during 3 months of observation on sandy soil of Mount Amba (at the University of Kinshasa in the Democratic Republic of Congo). Height and collar diameter growth, underground and total biomass dry weight, number and dry pea nodules and nodulation index have been evaluated at 4, 6, 8, 10 and 12 weeks of growth to assess the response of the legume to mineral nitrogen and organic nitrogen from chickens manure and pig feces. After observations, it appears that all the treatments soil showed different effects on growth and nodulation of pigeon pea. Therefore, only the soil received 1 kg of pig feces stimulated (p ≤ 0.05) the development of pigeon pea which resulted in good growth (nodulation 116.8 nodules and nodulation index of 5.0) and biomass production (25.3 gr per plant) while the addition of chicken manure, whatever the dose, reduced them.
基金Supported by Science and Technology Major Projects of Guangxi Province(GKAA17204057)Systematic Research Project of Guangxi Key Laboratory of Veterinary Biotechnology(14-045-31-A-5)Fundamental Research Fund of Guangxi Veterinary Research Institute(GKZX 2016-2)
文摘[Objective] The paper was to provide a basis for scientific prevention and control of pigeon Newcastle disease(ND).[Method] The HN gene of eight pigeon NDV strains isolated from different pigeon farms in Guangxi were amplified by RT-PCR,sequenced and analyzed.The molecular evolution characteristics of HN gene of pigeon NDV isolates in Guangxi was discussed.[Result] The nucleotide sequence length of HN gene of the eight NDV isolates was 1 716 bp,encoding 571 amino acids.They belonged to virulent group C,and the gene length characteristic of HN gene accorded with virulent strain.Analysis of nucleotide homologies indicated that the eight NDV isolates shared higher homology with genotype VIb,ranging from 90.4% to 99.5%.Phylogenetic tree analysis demonstrated that the genetic relationship between the eight NDV strains in Gangxi and the NDV isolates from Guangxi,Guangdong,Jilin,Liaoning,Yunnan and Heilongjiang during 2011 and 2013 was close.They were located in the same cladogram branch.[Conclusion] We assume that the eight pigeon NDV isolates in Guangxi all belong to the gene class II genotype VI b NDV.
文摘Today, the green pigeon (Treron calvus, TEMMINCK, 1811) holds an in-creasingly important place in the dietary habits of the populations of south-east of C?te d’Ivoire, especially those of Grand-Bassam. To provide food security to these customers a study was carried out. Objective: The current study is to identify the different protozoa and fungus that could po-tentially infect the green pigeon’s gut. Materials and Methods: The study was carried out on protozoa and digestive fungus in this species of pigeon in the south-east of C?te d’Ivoire. It has been carried out from November 2020 to February 2021. A total of sixty freshly dead pigeons, including 10 males and 50 females, have been bought in Grand-Bassam. After autopsy, the gut of each animal was preserved in formalin (10%) for a subsequent histopatho-logical examination. Results: The analysis of the results revealed the fol-lowing pathogen genus: Cryptosporidium spp, Histomonas spp, Toxoplasma gondii and Trichomonas spp for protozoa. Cryptococcus neoformans for fungus. The calculation of prevalence gave the following values: 63% [20;40] for protozoa and 37% [10;40] for fungus. Conclusion: The study showed that the intestine of the green pigeon of the Grand-Bassam region is infested by a range of pathogens. Namely: Cryptosporidium spp, Histomonas spp, Toxoplasma gondii, Trichonomas spp and Cryptococcus neoformans. Cryptococcus neoformans was the most common pathogen encountered in this study. Among these pathogens, some like Cryptococcus neoformans and Toxoplasma gondii are zoonosis. Thus, the present study deserves further investigation to know more about the nature of human infection in C?te d’Ivoire. Furthermore, given the diversity of digestive pathogens including zoonosis one, a reasonable recommendation to consumers would be stop eating the intestines.
文摘Aims: The aims of this work undertaken to investigate and identify the prevalence of Enterococcus spp isolated from Chicken and pigeon meat and determine the using antibiotics sensitivity against isolates for human safety. This study was conducted in center of excellence lab in Microbiology Department of Primeasia University, Banani, Dhaka-1213, Bangladesh during August, 2017 to March, 2018. Methodology and Results: Twenty four samples were collected in pre-sterilized zip-lock bags from different Gazipur area in different local meat vendors. All samples were transported to the Microbiology Laboratory of Primeasia University and analyzed according to standard analysis methods. Total viable count (TVC) and Total Enterocooci count (TEC) were estimated by using plate count agar (PCA) and Bile Esculineagar plates respectively. Various Selective media and Biochemical tests were used for presumptive isolation and identification. Kirby-Bauer disc diffusion method on Mueller Hinton agar media was used for the determination of sensitivity of the isolated strains to commonly prescribe antibiotics. A total of 24 meat samples were examined of which 92% were positive to Enterococcus spp whereas 62.5% were Chicken and 32.5% pigeon. The counts of enterococci range of 0 - 6.49 log CFU/gm in chicken and 4.4 - 6.4 log CFU/gm in pigeon. 22 isolates of enterococci, 77.27% were resistance to Azithromycin, 54.55% to Nalidixic acid and 50% to Sulfamethoxazole. We found high sensitivity of antimicrobial agent against to Imipenem (100%), Ciprofloxacin (81.81%) and (77.27%) both Tetracycline and Erythromycin. Conclusion: Significance and Impact of the study: The study result expressed that awareness of the meat pathogen and when consumption of meat was well cooked of our daily life.
文摘This study was aimed to determine the level of selected metals and nutritional composition of pigeon pea seed collected from seven districts of Wolaita zone. <span>A</span><span> </span><span>wet digestion procedure involving the use of mixtures of (69%</span><span> </span><span>-</span><span> </span><span>72%) HNO</span><sub><span style="vertical-align:sub;">3</span></sub><span> and (70%) HClO</span><sub><span style="vertical-align:sub;">4</span></sub><span> at an optimum temperature and time duration w</span><span>as</span><span> used to determine metals by using flame atomic absorption spectrometry. Kjeldahl digestion method, Soxhlet extraction and furnace were used to determine nutritional values of pigeon pea, and physicochemical properties of soils were assessed using standard methods. The results showed that the levels of concentration of metals in mg/kg dry weight were ranged 105.17 to 144.07 for K, 8.95 to 12.67 for Mg, 7.74 to 12.27 for Ca, 0.247 to 0.543 for Fe, 0.122 to 0.313 for Zn, 0.061 to 0.432 for Mn, 0.087 to 0.134 for Cu and 0.0011 to 0.00196 for Cr. The proximate composition of pigeon pea was in the range of 19.28% to 25.79% for crude protein, 0.993% to 1.75% for crude fat, 3.75% to 5.31% for ash, 10.65% to 13.73% for moisture, 2.28% to 3.06% for fiber, 54.36% to 60.1% for carbohydrate and 326.8 to 345.23 Kcal for energy. The pH of the soil was in the range from pH 5.09 (strongly acidic) to 6.77 (slightly acidic), EC of the soil ranged from 0.047 to 0.14 dS/m (low)</span><span>,</span><span> the soil OC level was from 1.6% to 2.42% (moderate), total Nitrogen was from 0.12% to 0.23% (low to moderate), the available Phosphorus content of the soil </span><span>wa</span><span>s from 6.82 to 13.52 mg/kg (low to moderate), CEC value of the soil </span><span>wa</span><span>s from 14.8 to 23.53 meq/100g (moderate). The textural classes of soil were sandy clay loam for all sites except Abela abaya. The study confirmed that pigeon pea </span><span>wa</span><span>s a good source of proteins, carbohydrates, and selected metals such as Mg, K, Ca, Fe, Zn, Mn, Cu, and Cr. The concentration of metals and nutritional compositions of pigeon pea seed were found at a permissible level.</span>
基金conducted as a part of a project on endemic birds in the Western Ghats,funded by the Ministry of Environment and Forest, Government of India(23-1/2001-RE)
文摘Foraging ecology of the globally endangered Nilgiri Wood Pigeon(Columba elphinstonii) was investigated in the Western Ghats,India,using faecal sampling and direct observations.The birds fed on fruits of 39 plant species,on seeds of 11 species,flowers and leaf buds of four species and a few ground invertebrates.Direct observations across seasons showed nine plant species were selected more often(47% of observations),with fruits of the members of the family Lauraceae the most preferred.Gleaning accounted for 76% of feeding methods throughout the year.Feeding was mainly from twigs at the edges on the upper and middle canopy(> 6 m).Frequency of feeding on fruits correlated significantly with fruit abundance,which depended on rainfall.The present study indicates that the pigeon requires a wide variety of diets from different forest environments.Conservation of this species depends on preserving all habitat types required to meet their resource needs throughout the year.
文摘试验旨在探究“2+2”生产模式下不同粗蛋白质水平对白王种鸽繁殖性能和乳鸽生长性能的影响。试验选取产蛋性能相近的300日龄健康种鸽168对,随机分为4组,每组7个重复,每个重复6对种鸽,每对种鸽哺育2只乳鸽。试验Ⅰ组、Ⅱ组、Ⅲ组和Ⅳ组分别饲喂粗蛋白质水平为13.43%、15.04%、16.64%和18.25%的饲粮。试验期46 d,其中孵化期18 d (1~18 d),哺乳期28 d (19~46 d)。结果显示,试验Ⅱ组、Ⅲ组产蛋间隔显著低于试验Ⅰ组(P<0.05),试验Ⅲ组种蛋受精率显著高于试验Ⅰ组、Ⅳ组(P<0.05),试验Ⅲ组入孵蛋孵化率极显著高于试验Ⅰ组、Ⅱ组、Ⅳ组(P<0.01)。试验Ⅲ组乳鸽28日龄体重显著高于试验Ⅰ组(P<0.05),乳鸽屠宰率极显著高于试验Ⅰ组(P<0.01),乳鸽胸肌率显著高于试验Ⅰ组、Ⅱ组、Ⅳ组(P<0.05)。试验Ⅱ组、Ⅲ组、Ⅳ组窝料重比1极显著低于试验Ⅰ组(P<0.01)。研究表明,不同粗蛋白质水平影响了种鸽的繁殖性能、乳鸽的生长性能以及窝料重比,在“2+2”生产模式下,建议白王种鸽饲粮适宜粗蛋白质水平为16.64%。