Effect of salviae miltiorrhizae and ligustrazine hydrochloride injection on axonal regeneration and nerve growth factor expression in a rat model of sciatic nerve injury

BACKGROUND： Studies have shown that both salviae miltiorrhizae and ligustrazine can promote protein expression of nerve growth factor （NGF） and regeneration of peripheral nerve. OBJECTIVE： To verify the effect of salviae miltiorrhizae and ligustrazine hydrochloride injection on axonal regeneration and NGF protein expression in a rat model of sciatic nerve injury. DESIGN, TIME AND SETTING： A randomized, controlled, animal experiment was performed at the Laboratory of Traditional Chinese Medicine and the Institute of Bioengineering of Jinan University from July to December 2008. MATERIALS： Salviae miltiorrhizae and ligustrazine hydrochloride injection （containing 20 mg salviae miJtiorrhizae and 100 mg ligustrazine per 100 mL injection） was provided by Guizhou Baite Pharmaceutical, China; salviae miltiorrhizae and ligustrazine decoctions （containing 1 g raw drug per 1 mL decoction） were provided by Guangzhou Baiyunshan Factory for Traditional Chinese Medicine, China; rabbit-anti-rat NGF monoclonal antibody was provided by Beijing Biosynthesis Biotechnology, China. METHODS： A total of 80 healthy, male, Sprague Dawley rats were used to establish a sciatic nerve injury model via neurotomy, and were then randomly assigned to 4 groups： salviae miltiorrhizae and ligustrazine hydrochloride injection group （intraperitoneal injection of 35 mL/kg per day salviae miltiorrhizae and ligustrazine hydrochloride injection）, saIviae miltiorrhizae group （intragastric peffusion of 2 mL salviae miltiorrhizae）, ligustrazine group （intragastric peffusion of 2 mL ligustrazine）, and model group （intraperitoneal injection of 35 mL/kg per day saline）, with 20 rats in each group. Thereafter, rats in each group were then divided into 4 subgroups according to varying time points of 1, 2, 4, and 8 weeks post-surgery, with 5 rats in each subgroup. MAIN OUTCOME MEASURES： Axons were quantified using chromotrope 2R-brilliant green and silver staining combined with image analysis to calculate the axonal regeneration rate; NGF expression was detected using immunohistochemistry and Western blot analysis; toe interspace was measured by behavior at 4 and 8 weeks. RESULTS： With increasing time after sciatic nerve expression, and toe interspace gradually increased njury, the axonal regeneration rate, NGF protein At 4 and 8 weeks post-surgery, axonal regeneration rate and NGF protein expression were significantly increased in the injured tissue of the salviae miltiorrhizae and ligustrazine hydrochloride injection, salviae miltiorrhizae, and ligustrazine groups, compared with the model group （P 〈 0.05 or P 〈 0.01）, and toe interspace was remarkably enlarged （P 〈 0.05 or P 〈 0.01）, especially in the salviae miltiorrhizae and ligustrazine hydrochloride injection group. CONCLUSION： Salviae miltiorrhizae and ligustrazine hydrochloride injection promoted axonal regeneration and NGF protein expression in the injured sciatic nerve, and also enhanced neurofunctional recovery. Its effect was superior to salviae miltiorrhizae or ligustrazine alone.

Clinical study of adjuvant therapy on ischemic stroke with salviae miltiorrhizae and ligustrazine

Objective: To study the effect of adjuvant therapy with salviae miltiorrhizae and ligustrazine injection on neural function injury, degree of oxidative stress reaction, chemotactic factor and adhesion molecule in patients with ischemic stroke. <br> Methods: Patients with ischemic stroke admitted in our hospital from August 2013 to October 2015 were retrospectively analyzed and divided into conventional treatment (CT) group receiving conventional treatment and traditional Chinese medicine auxiliary (TCMA) group receiving adjuvant therapy with salviae miltiorrhizae and ligustrazine injection. After the treatment of the first month and the third month, serum was collected to detect the contents of nerve injury molecules, oxidative stress parameters, chemotactic factor and adhesion molecule. <br> Results: After the 4-week treatment, the contents of serum neuron specific enolase, S100 calcium-binding protein B, heart-type fatty acid binding protein, malondialdehyde, advanced oxidation protein products, 8-hydroxy-2'-deoxyguanosine, monocyte chemoattractant protein-1,CD40,CD40 ligand, vascular endothelial cadherin, soluble intercellular adhesion molecule 1 and soluble vascular adhesion molecule 1 in patients ofTCMA group were all significantly lower than those of CT group, and the contents of superoxidase dismutase, glutathione peroxidase and catalase were significantly higher than those of CT group. After the 8-week treatment, the contents of serum neuron specific enolase, S100 calcium-binding protein B, heart-type fatty acid binding protein, malondialdehyde, advanced oxidation protein products, 8-hydroxy-2'-deoxyguanosine, monocyte chemoattractant protein-1,CD40, CD40 ligand, vascular endothelial cadherin, soluble intercellular adhesion molecule 1 and soluble vascular adhesion molecule 1 in patients ofTCMA group were all significantly lower than those of CT group and the contents of superoxidase dismutase, glutathione peroxidase and catalase were significantly higher than those of CT group. <br> Conclusions: Adjuvant therapy with salviae miltiorrhizae and ligustrazine injection can alleviate the neural function injury, inhibit oxidative stress reaction and the generation of chemotactic factor and adhesion molecule in patients with ischemic stroke, which is an effective medicine for treating ischemic stroke.

Microwave digestion polarography for determining seven trace elements in Salvia Miltiorrhiza Root and compound Salvia Miltiorrhiza Root injection simultaneously

The sensitive second derivative waves of Cu(Ⅱ),Pb(Ⅱ),Cd(Ⅱ),Ni(Ⅱ),Zn(Ⅱ),Fe(Ⅱ)and Mn(Ⅱ),forming in the substrate solution(pH=9.26)consisting of ammonia,ammonium chlorid,gelatin and ascorbic acid were researched.The peak potentials of the polarographic waves of the seven ions are at about 0.48,0.63,0.79,1.04,1.28,1.44 and 1.60 V respectively.Combining with microwave technique,a new method for the simultaneous determination of the seven trace elements in Chinese traditional medicine was developed.The method is easy to operate,rapid,simple and convenient.When the signal-to-noise rate equals 3,the detection limits of Cu,Pb,Cd,Ni,Zn,Fe and Mn are 4.2×10 3,5.3×10 3,2.1×10 3,5.8×10 4,3.0×10 3,7.7×10 4 and 1.1×10 3 μg/mL respectively.Well linear relationships exist between the concentrations and the peak currents when Cu,Pb,Cd,Ni,Zn,Fe and Mn concentrations are within 8.5×10 3 10,9.7×10 3 10,4.5×10 3 10,1.2×10 3 10,6.4×10 3 10,1.5×10 3 10 and 2.8×10 3 10 μg/mL,respectively.The method has been used to the simultaneous determination of the seven trace elements in Salvia Miltiorrhiza Root and compound Salvia Miltiorrhiza Root injection,the relative standard deviations(RSDs)of the Cu,Pb,Cd,Ni,Zn,Fe and Mn in the two medicines are 3.9% and 5.8%,4.0% and 4.1%,4.3% and 5.7%,4.9% and 5.3%,4.4% and 4.7%,3.5% and 4.0%,0.51% and 2.8%,respectively;the comparisons of the determination results with the values obtained by the standard method indicate that the presented method has very well veracity.

Effect of salvia miltiorrhiza and ligustrazine hydrochloride injection combined with hydroxyethyl starch injection on serum BNP, Hcy, MMP-2, S100B protein and hemorheology in patients with acute cerebral watershed infarction

Objective:To study the effect of salvia miltiorrhiza and ligustrazine hydrochloride injection combined with hydroxyethyl starch injection on serum BNP, Hcy, MMP-2, S100B protein and hemorheology in patients with acute cerebral watershed infarction.Methods:A total of 90 patientswith acute cerebral watershed infarction in our hospital from August 2014 to December 2016 were enrolled in this study. The subjects were divided into the control group (n=45) and the treatment group (n=45) randomly. The control group was treated with hydroxyethyl starch injection, the treatment group was treated withsalvia miltiorrhiza and ligustrazine hydrochloride injection combined with hydroxyethyl starch injection, and both the two groups were treated for 2 weeks. The serum BNP, Hcy, MMP-2, S100B protein and hemorheology of the two groups before and after treatments were compared.Results:There were no significantly differences of the serum BNP, Hcy, MMP-2, S100B protein and hemorheology of the two groups before treatment. The serum BNP, Hcy, MMP-2, S100B proteinlevels of the two groups after treatment were significantly lower than before treatment, and that of the treatment group after treatment were significantly lower than the control group. The PV, Lr, Mr, Hr and RE of the two groups after treatment were significantly lower than before treatment, and that of the treatment group after treatment were significantly lower than the control group.Conclusion:Salvia miltiorrhiza and ligustrazine hydrochloride injection combined with hydroxyethyl starch injectioncan significantlyimprovetheneurological function and hemorheology, reduce inflammation of the patients with acute cerebral watershed infarction, and it was worthy clinical application.

TREATMENT OF 40 CASES OF CHRONIC ECZEMA WITH POINT-INJECTION OF COMPOUND RADIX SALVIAE MILTIORRHIZAE INJECTION

The authors have used point-injection of compound Radix Salviae Miltiorrhizae Injection to treat 40 cases of chronic eczema and achieved a satisfactory therapeutic effect. The total effective rate was 95%.

CLINICAL ANALYSIS ON TREATMENT OF 40 CASES OF HYPERLIPEMIA WITH POINT-INJECTION OF RADIX SALVIAE MILTIORRZHIZAE INJECTION

In the present paper, the effect of point-injection of Radix Salviae Miltiorrhizae injectio on blood-lipid was observed in 65 cases of hyperlipemia. The 65 patients were divided into point-injection group (40 cases who were treated with point-injection of Radix Salviae Miltiorrhizae injectio and oral administration of Shujiangzhi) and control group (25 cases who were treated with oral administration of Shujiangzhi only). Results showed that the total effective rate of the point-injection group was significantly higher than that of the control group (P<0. 05); point-injection of Radix Salviae Miltiorrhizae injectio could effectively raise the level of HDL-C, decrease lower LDL-C and regulate estrogen level of women. The slow releasing action of red sage root solution in the topical acupoint region prolonged and enhanced its effect in lowering blood-lipid. There was a significant difference between the two groups in the effect of lowering blood-fat (P< 0. 01).

Effect of adjuvant salvia miltiorrhiza and ligustrazine therapy on renal function, renal blood perfusion as well as CTGF and TGF-β1 content in patients with chronic renal failure

Objective:To analyze the effect of adjuvant salvia miltiorrhiza and ligustrazine therapy on renal function, renal blood perfusion as well as connective tissue growth factor (CTGF) and transforming growth factor (TGF)-β1 content in patients with chronic renal failure.Methods:80 patients with chronic renal insufficiency treated in our hospital between March 2013 and March 2016 were selected for study and randomly divided into observation group (n=40) and control group (n=40). Control group received conventional therapy and observation group received conventional + adjuvant salvia miltiorrhiza and ligustrazine therapy. After 3 months of treatment, differences in renal function indexes, illness-related indexes, renal blood perfusion, CTGF and TGF-β1 content, and so on of two groups of patients were determined. Results: After 3 months of treatment, serum urea nitrogen (BUN), serum creatinine (Scr),β2 microglobulin (β2-MG), intermedin (IMD), fibroblast growth factor 23 (FGF23), cystatin C (CysC), CTGF and TGF-β1 content as well as 24 h urine albumin excretion rate (UAER) level in urine of observation group were significantly lower than those of control group (P<0.05) while glomerular filtration rate (GFR) level and serum adiponectin (APN) content were significantly higher than those of control group (P<0.05);renal perfusion parameters renal cortex Tmax (ATc) and medulla Tmax (ATm) levels of observation group were significantly lower than those of control group while cortex peak intensity change (ΔAc), medulla peak intensity change (ΔAm) and peak intensity (PI) levels were significantly higher than those of control group.Conclusions:Adjuvant salvia miltiorrhiza and ligustrazine therapy can effectively control the overall condition of patients with chronic renal failure, and plays a positive role in improving renal function and increasing renal blood perfusion.

Effect of salvia miltiorrhiza injection adjuvant therapy on systemic inflammatory stress state and nerve injury degree of children with bacterial Encephalitis

Objective:To discuss the effect of salvia miltiorrhiza injection adjuvant therapy on systemic inflammatory stress state and nerve injury degree of children with bacterial meningitis. Methods: 68 cases of children with bacterial meningitis who were treated in our hospital from Feb. 2014 to Jan. 2017 were selected and were divided into control group (34 cases) and observation group (34 cases) based on random number table, and patients in both groups were treated with continuous treatment for 10 d. Differences of inflammatory mediator, oxidative stress index, nerve injury markers content in cerebrospinal fluid in both groups were compared. Results: Before treatment, differences of inflammatory mediator, oxidative stress index, nerve injury markers content in cerebrospinal fluid in both groups had no statistical significance. 10 d after treatment, inflammatory mediator IL-6, PCT, sVCAM-1, CRP content in cerebrospinal fluid of children in observation group was lower than that in control group;oxidative stress index MDA content in cerebrospinal fluid was lower than that in control group, and SOD content was higher than that in control group;nerve injury markers MBP, NSE, GFAP content in cerebrospinal fluid was lower than that in control group.Conclusion: salvia miltiorrhiza injection adjuvant therapy could effectively relieve the systemic inflammatory stress state and reduce the nerve damages of children with bacterial meningitis.

Experimental Study on Therapeutic Effect of Combined Administration of Radix Salviae Miltiorrhizae. Ligustrazine and Radix Sanchi on Late Hemorrhagic Shock of Rabbits

Objective: To explore the therapeutic mechanism of the combined use of Radix Salviae Miltiorrhizae, ligustrazine and Radix Sanchi in treating late hemorrhagic shock in rabbit. Methods: Rabbit hemorrhagic shock models were set up according to Wiggers' method and treated with the combined therapy. Levels of RBC superoxide dismutase (SOD) activity, plasma malondialdehyde (MDA), blood lactate (BL) and magnesium (Mg++ ) were continuously monitored before shock, 120 minutes after shock, 60 and 120 minutes after reinfusion. Results: (1) Levels of SOD decreased and MDA, AL, Mg++ increased markedly 120 minutes after shock, indicating that there existed cell membrane damage caused by oxygen free radicals in hemorrhagic shock.(2) The combination therapy could alleviate lipid peroxidation injury to tissue, enhance the activity of SOD and lower the concentration of MDA significantly, P < 0. 01 or 0. 05, as compared with the control group. Conclusion: The combined use of the three drugs could gain the same effect with half dosage as that gained from the whole dosage of one single drug. It could also reduce the negative effect of treatment, such as hypotension and slowing down of heart rate

Effect of Salvia miltiorrhiza Injection Combined with Laparoscopic Reduction on Injury of Appendix in Patients with Intussusception

[Objectives] The aim was to analyze the effect of Salvia miltiorrhiza injection combined with laparoscopic reduction on the degree of appendix injury in intussusception.[Methods]Seventy-four children with intussusception who were treated in the Xuchang Municipal Hospital from August 2014 to August 2017 were randomly and evenly divided into two groups,control group and treatment group.The children in the control group were treated by laparoscopic reduction,and those in the treatment group were treated with S.miltiorrhiza injection combined with laparoscopic reduction.The clinical effect on intussusception in the children was compared between the two groups.[Results] Before treatment,no significant difference was founding serum NO level between the control and treatment groups(P > 0.05).On Day 1,3 and 5 of treatment,the serum NO levels of the treatment group[(74.16 ± 6.81),(61.98 ± 4.77) and(54.76 ± 4.36) μmol/L] were significantly lower than those of the control group[(89.67 ± 6.34),(84.75 ± 5.21) and(71.95 ± 4.58) μmol/L](P < 0.05).After treatment,the TNF-α and IL-6 levels of the treatment group[(184.32 ± 10.67) and(332.16 ± 34.17) pg/m L] were significantly lower than those of the control group[(268.26 ± 12.74) and(438.23 ± 35.26) pg/m L](P < 0.05);and the Chiu score of the treatment group(2.34 ± 1.12) was also significantly lower than that of the control group(4.85 ± 1.67)(P < 0.05).[Conclusions]For children with intussusception,S.miltiorrhiza injection combined with laparoscopic reduction is effective in reducing serum NO level and reducing the degree of appendix injury,which is worthy of clinical application.

Rapid separation and identification of multiple constituents in Danhong Injection by ultra-high performance liquid chromatography coupled to electrospray ionization quadrupole time-of-flight tandem mass spectrometry

To characterize and identify multiple constituents in Danhong injection(DHI), a fast ultra-high performance liquid chromatography coupled to electrospray ionization quadrupole time-of-flight tandem mass spectrometry(UHPLC-ESI-QTOF/MS) method was established and validated in the present study. A total of 63 compounds, including 33 phenolic acids, 2 C-glycosyl quinochalcones, 6 flavonoid O-glycosides, 4 iridoid glycosides, 6 organic acids, 5 amino acids, and 3 nucleosides, were identified or tentatively characterized. In conclusion, the UHPLC-ESI-QTOF/MS method is useful and efficient for in-depth structural elucidation of chemical compounds in complex matrices of herbal medicines such as DHI.

Anti-fibrotic Effects of Salvia miltiorrhiza and Ligustrazine Injection on LX-2 Cells Involved with Increased N-myc Downstream-Regulated Gene 2 Expression

Objective: To investigate the effects of Salvia miltiorrhiza and Ligustrazine Injection（SML） on proliferation and apoptosis of human hepatic stellate cell LX-2 and the expression of N-myc downstreamregulated gene 2（NDRG2, a tumor suppressor gene）. Methods: HSCs from the LX-2 cell line were cultured in vitro. The proliferative state of different initial LX-2 cell numbers was measured using a 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT） colorimetric assay. LX-2 cells were plated in 96-well plates at an approximate density of 2.50×10;cells/mL and cultured for 24 h followed by the application of different concentrations of SML（1, 2, 4 and 8 μL/mL）. Cell proliferation was measured using the MTT assay at 24 and 48 h. Apoptosis was detected by flow cytometry at 24 h. LX-2 cells were treated with different concentrations of SML and extracted with protein lysis buffer. The levels of NDRG2 and β-catenin were measured by Western blot. Results: With the exception of the 1 and 2 μL/mL concentrations, 4 and 8 μL/mL SML inhibited cell proliferation in a concentration-dependent manner at 24 and 48 h（P<0.05）. With the exception of the 1 and 2 μL/mL concentrations, the NDRG2 expression level was greatly increased in a concentration-dependent manner. However, the level of β-catenin was unaffected. Conclusion: SML inhibit LX-2 cell proliferation in a concentration-dependent manner, and the mechanism may be associated with NDRG2 over-expression.

丹参川芎嗪联合针刺疗法对急性颅脑损伤患者神经功能及炎症因子水平的影响

目的观察丹参川芎嗪联合针刺疗法对急性颅脑损伤患者神经功能及炎症因子表达水平的影响。方法选取2022年9月至2023年9月在湖南中医药高等专科学校附属第一医院急诊科住院的急性颅脑损伤患者80例,按照随机数表法将其分为对照组和观察组,每组各40例。对照组予以西医常规治疗;观察组在对照组基础上予以丹参川芎嗪注射液5 ml,1次/d,静脉滴注,并配合针刺疗法,两组疗程均为7 d。观察两组患者治疗前后的肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、C反应蛋白(CRP)、日常生活能力(ADL)评分、神经功能评分(NIHSS)变化。结果80例患者在治疗观察过程中脱落5例,最终入组75例,对照组37例,观察组38例。治疗后,两组总有效率比较,差异无统计学意义(P>0.05)。治疗后,观察组NIHSS及ADL评分低于对照组,差异有统计学意义(P<0.05);观察组IL-6、IL-8、TNF-α及CRP表达水平低于对照组,差异有统计学意义(P<0.05)。结论丹参川芎嗪注射液联合针刺疗法能够有效降低急性颅脑损伤患者的炎症因子水平,减轻患者神经功能缺损,提高患者日常生活自理能力,临床疗效良好。

Effects of Salivae Miltiorrhizae Liguspyragine Hydrochloride and Glucose Injection(参芎葡萄糖注射液)on the Levels of Main Platelet Thrombin Receptors in Chronic Haemodialysis Patients

Objective：To investigate the effects of Salvia Miltiorrhiza Liguspyragine Hydrochloride and Glucose Injection（参芎葡萄糖注射液,SLGI） on the expression of platelet membrane receptors proteinase-activated receptor-1（PAR1） and proteinase-activated receptor-4（PAR4） in end-stage renal disease（ESRD） patients on chronic haemodialysis（HD）.Methods：Eighty-six ESRD patients on HD（treated group） were treated with SLGI,7 days as one therapeutic course,for two successive courses.The previous therapies were unchanged.Flow cytometry was used to assess the expression of platelet PAR1 and PAR4 in the patients,and turbidity method was used to determine the platelet maximum aggregation rate（MAR）.Meanwhile,renal function was measured.The final data were compared with those before treatment and with those in the normal control group（54 healthy subjects）.Results：Compared with the normal control group,the expressions of PAR1 and PAR4 and platelet MAR in ESRD patients on HD was significantly higher before treatment（P=0.001,P=0.006, and P=0.008）;after treatment with SLGI,the above indices in patients were remarkably decreased（P=0.036 and P=0.046）,except PAR4（P=0.067）,but still higher than those in the normal control group,however,it was not statistically significant.Conclusions：（1） The overexpression of PAR1 and PAR4 might lead to increased platelet aggregation and this could be one of the reasons for the thrombotic events in ESRD patients on HD.（2） SLGI was able to down-regulate the expression of PAR1 in ESRD patients on HD,improve platelet function,and regulate platelet activation.

Observation on the Effect of Salvia Miltiorrhiza Injection in Treating Traumatic Hyphema and the Opportune Time for Its Application

Objective： To observe the effect of Salvia miltiorrhiza Injection （SMI） in treating traumatic hyphema （TH） and the opportune time for its application. Methods： A retrospective study was conducted in 174 patients with TH （all with a single eye wounded）, of whom 92 patients were treated with dicynone and 82 with SMI, and their status of recurrent or aggravated hemorrhage within 5 days after trauma were analyzed. Further, a prospective study was conducted in 76 TH patients （all with a single eye wounded）, who were treated with dicynone though they had hyphema 5 days after treatment. After the dicynone medication was discontinued, 39 of them were treated with Sl, but 37 were not. The status of hyphema absorption was observed. Results： The retrospective study showed that the number of cases with recurrent or aggravated bleeding in the SMI-treatment group was much more than that in the dicynone- treated group （Z=-2.531, P=0.011）. On the other hand, the prospective study showed that the status of hyphema absorption among the SMI-treated patients was significantly better than in those untreated with SMI （Z=-2.642, P=0.008）. Conclusion： SMI shows an effect of promoting the absorption of TH, and SMI treatment is worthy of being spread in clinical practice. For safety considerations, it is suitable to apply the treatment 5 days after trauma.

丹参川芎嗪注射液联合厄贝沙坦分散片治疗原发性高血压的临床效果

目的:探究丹参川芎嗪注射液联合厄贝沙坦分散片治疗原发性高血压的效果,并分析其对颈动脉内膜-中层厚度(IMT)、血管内皮功能的影响。方法:选取2020年9月—2022年9月在山东省泰安荣军医院治疗的原发性高血压患者86例,以随机数字表法将其分为对照组(n=43)及观察组(n=43)。对照组接受厄贝沙坦分散片治疗,观察组接受丹参川芎嗪注射液联合厄贝沙坦分散片治疗。对比两组血压水平[收缩压(SBP)、舒张压(DBP)]、IMT、动脉弹性功能[臂踝脉搏波传导速度(baPWV)、踝臂指数(ABI)、大动脉弹性指数(C1)、小动脉弹性指数(C2)]、血管内皮功能[一氧化氮(NO)、脂质过氧化物(LOP)、内皮素-1(ET-1)]、血清炎症因子[白细胞介素-6(IL-6)、单核细胞趋化蛋白-1(MCP-1)]。结果:治疗前,两组SBP、DBP、IMT比较,差异均无统计学意义(P>0.05);治疗后,两组SBP、DBP、IMT水平均降低,观察组均低于对照组,差异均有统计学意义(P<0.05)。治疗前,两组baPWV、ABI、C1、C2比较,差异均无统计学意义(P>0.05);治疗后,两组baPWV均降低,C1、C2均升高,观察组baPWV低于对照组,C1、C2均高于对照组,差异均有统计学意义(P<0.05);两组治疗后ABI比较,差异无统计学意义(P>0.05)。治疗前,两组NO、LOP、ET-1比较,差异均无统计学意义(P>0.05);治疗后,两组NO水平均升高,LOP、ET-1水平均降低,观察组NO高于对照组,LOP、ET水平均低于对照组,差异均有统计学意义(P<0.05)。治疗前,两组IL-6、MCP-1比较,差异均无统计学意义(P>0.05);治疗后,两组IL-6、MCP-1水平均降低,观察组均低于对照组,差异均有统计学意义(P<0.05)。结论:采用丹参川芎嗪注射液联合厄贝沙坦分散片治疗原发性高血压,血压控制较好,且可改善血管内皮功能及动脉弹性功能,降低IMT和炎症因子水平。

重组人脑利钠肽联合丹参川芎嗪注射液治疗慢性心力衰竭的效果研究

目的分析慢性心力衰竭(CHF)采用重组人脑利钠肽(rhBNP)联合丹参川芎嗪注射液(SMLI)治疗取得的效果。方法86例CHF患者,参照随机数字表法分为对照组和研究组,每组43例。对照组患者采用常规对症治疗与rhBNP治疗,研究组患者在对照组的基础上联用SMLI治疗。对比两组疗效、心功能指标(左心室收缩末期内径、左室射血分数、左心室舒张末期内径)、心力衰竭(心衰)血清学指标(N末端脑钠肽前体、心肌肌钙蛋白I)及不良反应发生情况。结果研究组总有效率95.35%较对照组的79.07%高(P<0.05)。研究组治疗后左心室收缩末期内径(44.65±4.50)mm、左心室舒张末期内径(51.67±2.79)mm小于对照组的(48.70±5.05)、(54.55±2.62)mm,左室射血分数(44.44±4.80)%高于对照组的(39.74±3.65)%(P<0.05)。研究组治疗后N末端脑钠肽前体(828.34±179.40)ng/L、心肌肌钙蛋白I(2.60±0.69)μg/L低于对照组的(970.25±112.32)ng/L、(4.50±0.75)μg/L(P<0.05)。两组不良反应发生率对比,结果未见差异性(P>0.05)。结论CHF患者采用rhBNP与SMLI联合治疗疗效确切,能够有效改善患者的心功能,调节心衰血清学指标,安全性较为理想,具有临床应用价值。

旴江谢氏刺营疗法配合丹参黏膜下注射治疗口腔 黏膜下纤维化的临床研究

目的探讨口腔黏膜下纤维化(OSF)患者采用旴江谢氏刺营疗法配合丹参黏膜下注射治疗的效果。方法将2020年1月至2022年6月该院收治的80例OSF患者随机分为观察组和对照组,每组各40例。对照组予以丹参黏膜下注射治疗,观察组予以旴江谢氏刺营疗法配合丹参黏膜下注射治疗,两组均治疗3个月。比较两组治疗效果、症状体征、血清指标水平。结果观察组治疗总有效率为95.00%,高于对照组的80.00%,差异有统计学意义(P<0.05);两组治疗后视觉模拟评分法评分、张口度和口腔黏膜病损面积均较治疗前改善,并且观察组较对照组改善情况更明显,差异均有统计学意义(P<0.05);两组治疗后血清转化生长因子-β1(TGF-β1)及白细胞介素-6(IL-6)水平均低于治疗前,并且观察组低于对照组,差异均有统计学意义(P<0.05)。结论OSF患者采用旴江谢氏刺营疗法配合丹参黏膜下注射治疗效果满意,可有效改善患者临床症状,调节血清TGF-β1、IL-6水平。

丹参川芎嗪注射液联合阿加曲班、阿替普酶静脉溶栓治疗急性脑梗塞的效果及对血小板因子、神经因子水平的影响

目的探讨丹参川芎嗪注射液联合阿加曲班、阿替普酶静脉溶栓治疗急性脑梗塞的效果及对血小板因子、神经因子水平的影响。方法选取2021年1月至2022年12月就诊的100例急性脑梗塞患者为研究对象,根据治疗方案不同将其分为对照组与观察组,各50例。对照组采用阿加曲班、阿替普酶静脉溶栓治疗,观察组在对照组基础上加用丹参川芎嗪注射液治疗。比较两组的治疗效果。结果观察组的治疗总有效率高于对照组(P<0.05)。治疗后,观察组的血小板活化因子(PAF)、α颗粒膜糖蛋白-140(GMP-140)、β-血小板球蛋白(β-TG)、血小板第4因子(PF4)水平均低于对照组(P<0.05)。治疗后,观察组的神经元特异性烯醇化酶(NSE)、胶质纤维酸性蛋白(GFAP)水平低于对照组,神经生长因子(NGF)、脑源性神经营养因子(BDNF)水平高于对照组(P<0.05)。结论丹参川芎嗪注射液联合阿加曲班、阿替普酶静脉溶栓可调节急性脑梗塞患者的血小板因子及神经因子水平,促进神经功能恢复,疗效显著,值得推广。

丹参川芎嗪与阿托伐他汀钙联合用药方案治疗急性心肌梗死的效果评价

目的探讨丹参川芎嗪联合阿托伐他汀钙治疗急性心肌梗死的疗效,为临床医师选择合理用药方案提供参考。方法选取2019年1月1日至2019年12月31日沈阳七三九医院诊治的急性心肌梗死患者52例作为研究对象,依据随机数字表法,将研究样本均分为两组,分别为对照组与观察组,每组26例。在基础治疗的前提下,对照组采用阿托伐他汀钙,观察组则同时应用丹参川芎嗪与阿托伐他汀钙。对比分析两组患者治疗效果以及心功能指标的改变。结果观察组的临床疗效(92.31%)优于对照组(69.23%)(P=0.035)。治疗后,观察组的心功能指标,包括每搏输出量、中心静脉压及左室射血分数,均较治疗前明显改善(P<0.05),且三项指标均优于对照组(P<0.05)。结论丹参川芎嗪与阿托伐他汀钙联合用药方案治疗急性心肌梗死的效果明显,能改善患者心功能。