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A signal-amplified whole-cell biosensor for sensitive detection of Hg^2+ based on Hg^2+-enhanced reporter module 被引量:1
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作者 Dan Wang Yanan Zheng +4 位作者 Liudan Wei Na Wei Xiaosu Fan Shan Huang Qi Xiao 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2020年第10期93-98,共6页
A signal-amplified mercury sensing biosensor with desired sensitivity was developed through firstly using the GFP mutant with fluorescence increasing response towards Hg^2+ as the reporter module.The developed biosens... A signal-amplified mercury sensing biosensor with desired sensitivity was developed through firstly using the GFP mutant with fluorescence increasing response towards Hg^2+ as the reporter module.The developed biosensor showed response for Hg^2+ in a relatively wide range of 1–10,000 nmol/L,and the detection limit was improved one or two orders of magnitude in comparison with most metal-sensing biosensors in similar constructs.In addition,the biosensor could distinguish Hg^2+ easily from multiple metal ions and displayed strong adaptability to extensive p H conditions (pH 4.0–10.0).More importantly,the developed biosensor was able to provide an initial assessment of Hg^2+ spiked in the environmental water with the recoveries between 85.70%and 112.50%.The signal-amplified strategy performed by the modified reporter module will be widely applicable to many other wholecell biosensors,meeting the practical requirements with sufficient sensing performance. 展开更多
关键词 Mercury sensing biosensor Signal-amplified Reporter module Environmental samples monitoring
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