Inhibition of Sanggenon G Isolated from Morus alba on the Metastasis of Cancer Cell

Objective An organic layer prepared from the cortex of Morus alba(Moraceae)was studied in order to identify the active compounds for heparinase.Methods Bioassay-guided fractionation resulted in the isolation of sanggenon G.Results The compound showed inhibitory activity with IC50 of 3.7μmol/L on heparinase in vitro as well as 24μmol/L in invasion assay using MDA-MB231 cells.Sanggenon G also had the moderate cytotoxicity at SW 620(colon)and ACHN(kidney)cancer cell lines with IC50 of 10.96 and 13.44μmol/L,respectively.Conclusion This is the first time that prenylated flavonoid sanggenon G is described as heparinase inhibitor.Besides,this flavonoid would be expected to be a metastasis inhibitor of cancer cells and also a valuable reagent to explore the mechanism of heparinase/heparanase-mediated metastasis.

HPLC双波长法同时测定桑白皮水提物中6个成分的含量

目的:建立HPLC双波长法同时测定桑白皮水提物中桑皮苷A、绿原酸、单宁酸、桑根酮D、桑酮G、桑根酮C等6个主要成分的含量。方法:色谱柱为Phenomenex Gemini C18(250 mm×4.6 mm,5μm);以乙腈为流动相A,0.2%甲酸水溶液为流动相B,梯度洗脱;检测波长:270 nm(单宁酸、桑根酮D、桑酮G、桑根酮C)、320 nm(桑皮苷A、绿原酸);柱温:30℃;流速:1.0 mL·min^(-1);进样量:20μL。结果:桑皮苷A、绿原酸、单宁酸、桑根酮D、桑酮G、桑根酮C分别在3.24~647.20μg·mL^(-1)、3.22~643.60μg·mL^(-1)、2.50~588.80μg·mL^(-1)、3.28~656.80μg·mL^(-1)、3.19~637.20μg·mL^(-1)、3.10~619.60μg·mL^(-1)质量浓度范围内与峰面积线性关系良好(r>0.999);加样平均回收率分别为99.18%、99.06%、98.96%、98.76%、98.99%、98.90%(n=9),其RSD分别为0.45%、0.33%、0.59%、0.36%、0.47%、0.49%。结论:该方法的准确度、重复性、耐用性均良好,适合用于桑白皮水提物中桑皮苷A等6个主要成分的定量测定。