Sanguinarine Attenuates Lipopolysaccharide-induced Inflammation and Apoptosis by Inhibiting the TLR4/NF-KB Pathway in H9c2 Cardiomyocytes

The inflammatory response is involved in the pathogenesis of the most common types of heart disease. Sanguinarine （SAN） has various pharmacological properties such as anti-inflammatory, antioxidant, antibacterial, antitumor, and immune-enhancing properties. However, few studies have investigated the effects of SAN on lipopolysaceharide （LPS）-induced inflammatory and apoptotic responses in H9c2 cardiomyocytes. Therefore, in this study, H9c2 cells were co-treated with SAN and LPS, and the mRNA levels of pro-inflammation markers and the apoptosis rate were measured to clarify the effect of SAN on cardiac inflammation. The underlying mechanism was further investigated by detecting the activation of Toll-like receptor （TLR）4/nuclear faetor-κB （NF-κB） signaling pathways. As a result, increased mRNA expression of interleukin （IL）-1β, IL-6, and TNFα induced by LPS was attenuated after SAN treatment; LPS-induced apoptosis ofHge2 cardiomyocytes and cleaved-caspase 8, 9, 3 were all significantly reduced by SAN. Further experiments showed that the beneficial effect of SAN on blocking the inflammation and apoptosis of H9c2 cardiomyocytes induced by LPS was associated with suppression of the TLR4/NF-κB signaling pathway. It was suggested that SAN suppressed the LPS-induced inflammation and apoptosis of H9c2 cardiomyocytes, which may be mediated by inhibition of the TLR4/NF-κB signaling pathway. Thus, SAN may be a feasible therapy to treat sepsis patients with cardiac dysfunction.

Antitumor effects of the benzophenanthridine alkaloid sanguinarine: Evidence and perspectives

Historically, natural products have represented a significant source of anticancer agents, with plant-derived drugs becoming increasingly explored. In particular, sanguinarine is a benzophenanthridine alkaloid obtained from the root of Sanguinaria canadensis, and from other poppy Fumaria species, with recognized anti-microbial, anti-oxidant and anti-inflammatory properties. Recently, increasing evidence that sanguinarine exibits anticancer potential through its capability of inducing apoptosis and/or antiproliferative effects on tumor cells, has been proved. Moreover, its antitumor seems to be due not only to its pro-apoptotic and inhibitory effects on tumor growth, but also to its antiangiogenic and anti-invasive properties. Although the precise mechanisms underlying the antitumor activity of this compound remain not fully understood, in this review we will focus on the most recent findings about the cellular and molecular pathways affected by sanguinarine, together with the rationale of its potential application in clinic. The complex of data currently available suggest the potential application of sanguinarine as an adjuvant in the therapy of cancer, but further pre-clinical studies are needed before such an antitumor strategy can be effectively translated in the clinical practice.

Spectral studies of the interaction between sanguinarine and guanosine

The interaction between sanguinarine and guanosine was investigated by using UV-vis and fluorescence spectra at pH 7.2. The binding of sanguinarine to guanosine was substantiated by the hypochromism and bathochromism in the absorption spectra and the emission quenching in fluorescence spectra. The fluorescence lifetime results, the varieties of the fluorophore absorption spectra and the decrease of the binding constant with the increasing temperature all indicate that the fluorescence quenching is static. The ratio and constant of the binding cytidine to sanguinarine are 2 and 6.44 × 10^7, respectively. The result shows that the binding of sanguinarine to guanosine is not only exothermic but also entropy-driven with △H=-8.53kJ/mol, AS = 0.12 kJ/（molK）, and AG =-44.57 kJ/mol at 298.15 K.

Stability of Sanguinarine and Chelerythrine in Macleaya cordata (Willd.) R. Br.

[Objectives]To explore the stability of sanguinarine and chelerythrine in Macleaya cordata(Willd.)R.Br.[Methods]The solubility and stability of sanguinarine and chelerythrine in seven solvents were measured by HPLC.Besides,the effects of water quality,light source,oxidant,temperature,and pH on stability were investigated.[Results]The solubility and stability of sanguinarine and chelerythrine in methanol and ethanol are good;the stability of sanguinarine and chelerythrine in distilled water and rainwater is not affected by light and is very stable,but they are unstable in tap water whether they are protected from light or not;oxidants have a great influence on the stability of sanguinarine and chelerythrine;sanguinarine and chelerythrine are stable at room temperature lower than 54℃;sanguinarine is stable in pH 2.5-7.0,while chelerythrine is stable in pH 2.5-8.0.[Conclusions]The good stability of sanguinarine and chelerythrine under specific conditions shows that they have broad development prospects and value.

Simultaneous Quantitative Determination of Nitidine, Chelerythrine and Sanguinarine Using HPTLC from Callus Extract of <i>Zanthoxylum rhetsa</i>

Nitidine, Chelerythrine and Sanguinarine, all these three alkaloids are benzophenanthridine alkaloids. Nitidine was used as an anti-HIV, anti-malarial and anti-cancer. Chelerythrine had anti-cancer and anti-inflammatory activities. Sanguinarine was widely used as an anti-plaquestic and anti-cancer. High performance thin layer chromatography (HPTLC) method was used for simultaneous quantification of Nitidine, Chelerythrine and Sanguinarine in callus extract of Zanthoxylum rhetsa by using Silica gel 60 F254 as stationary phase and ethyl acetate:methanol:water:diethylamine (30:5:2:0.5 v/v) as mobile phase at 280 nm. The linearity concentration range was 5 - 160 μg/band of each alkaloid. The Rf values of Nitidine, Chelerythrine and Sanguinarine were found to be 0.28, 0.49 and 0.73. The limit of detection and limit of quantification were found to be 0.026, 0.088 μg/spot and 0.010 and 0.033 μg/spot, 0.0104 and 0.035 μg/spot respectively for Nitidine, Chelerythrine and Sanguinarine. HPTLC method was developed and validated according to ICH guidelines for simultaneous estimation of Nitidine, Chelerythrine and Sanguinarine and proved to be simple, specific, accurate, robust and rapid.

Sanguinarine-Mediated Sensitization of Cervical Cancer SiHa Cells to TRAIL

Introduction: Cervical cancer is primarily caused by the human papilloma virus (HPV), which transforms normal cervical cells into cancerous cells that are highly resistant to radiation and chemotherapy. Induction of apoptosis in transformed cells is a key strategy in successfully treating HPV-induced cervical cancer. TRAIL (tumor necrosis factor related apoptosis-inducing ligand) has been shown to selectively induce apoptosis in cancer cells by binding to death receptors and activating extrinsic pathways for apoptosis. However, certain cervical cancers—such as the cultured cell line SiHa—are remarkably resistant to TRAIL. In this study, SiHa cells were sensitized to TRAIL by using sanguinarine—derived from the plant Sanguinaria Canadensis—which is known to induce oxidative stress and lead to the upregulation of receptors for TRAIL. Methods: Cultured SiHa cells were exposed to sub-lethal doses of sanguinarine in combination with TRAIL. Cell viability changes as well as the production of reactive oxygen species (ROS) were assessed. The induction of apoptosis was investigated by assays for caspase activation. Flow cytometry was performed to analyze expression of death receptors 4/5. Results: Treatment of SiHa cells with a combination of sanguinarine and TRAIL led to a significant reduction in cell viability. Significant increase in ROS was observed and caspase activation assays confirmed the induction of apoptosis. Conclusions: The observed synergistic effect of sanguinarine and TRAIL on SiHa cells is promising for the treatment of cervical, and possibly other, HPV-induced cancers. Oxidative stress caused by sanguinarine seems to play a central role in this synergy. The precise link between reactive oxygen species and the possible upregulation of death receptors needs further investigation. This knowledge will enable us to devise more effective treatments for those who suffer from this devastating disease.

Systematic understanding of the underlying target and mechanism of sanguinarine against nasopharyngeal carcinoma through a networkpharmacology approach

Background:Nasopharyngeal carcinoma is a malignant tumor,well known as a cancer type characterized by regional specificity,especially in Southern China.The network pharmacology is an emerging discipline developed in recent years,which has been effectively used to predict the potential therapeutic compounds against disease focusing on the possible therapeutic targets and mechanisms.Sanguinarine,a traditional natural plant-derived phenanthridine alkaloid,has been reported to have a wide variety of pharmacological activities for decades.Methods:In the current study,using the comprehensive network pharmacological method,the potential drug targets of sanguinarine against nasopharyngeal carcinoma were successfully predicted,and verified by molecular docking.The underlying pharmacological mechanism was initially unraveled.Results:Totally,38 potential common targets were confirmed from these potential nasopharyngeal carcinoma therapeutic targets and pharmacological targets of sanguinarine.Their enrichment analyses of GO functions show that protein serine/threonine kinase activity,histone kinase activity,integrin binding,protein tyrosine kinase activity,and cell adhesion molecule binding were top listed.KEGG functional enrichment analysis indicates that the potential pathways are mainly involved into PI3K-Akt signaling pathway.The"drug-target-disease-pathway"network model diagram points out the key genes containing MAPK10,MAPK14,JAK2,BRAF,GSK3B,MET,HSP90AA1,SRC,et al..According to the results of molecular docking,it was further verified that sanguinarine has strong binding ability with MAPK10 and MAPK14.Conclusion:Taken together,this study would provide a clarified theoretical basis for the subsequent wet laboratory research focusing on sanguinarine against nasopharyngeal carcinoma.

PEI/SA/Cu三元体系改性荷正电纳滤膜及其脱盐性能

通过将海藻酸钠(SA)与聚乙烯亚胺(PEI)共混作为二次界面聚合反应的水相溶液,SA与PEI强相互作用使SA嵌入到聚酰胺层中,从而提高膜的致密性.随后通过Cu^(2+)与SA结合提高膜表面荷正电性,提高膜对多价阳离子的截留性能.对比研究了SA和Cu^(2+)浓度对复合膜物化性质、表面形貌及分离性能的影响.结果表明,荷正电纳滤膜的最佳制备条件为:SA质量分数为0.01%,Cu^(2+)浓度为25 mmol/L,所对应的PS/Cu-3复合膜亲水性提高.与Control-PEI膜相比,其通量提高了257%,为(40.0±0.8)L/(m^(2)·h),对MgCl2的截留率从92.0%提升至97.1%,且具有良好的耐压性和稳定性.

卫生健康标准中关系型数据共现矩阵计算及SAS程序实现

目的设计卫生健康标准中关系型数据共现矩阵的求解路径及其SAS实现方法。方法文章以计算一组标准的起草单位共现矩阵为例,将“标准-起草单位”二维表格导入SAS(版本号:9.4),按照“两两相乘”的计算思路自行设计宏程序计算起草单位之间的共现矩阵。结果以计算一组标准的起草单位共现矩阵为例,采用自行构建的模拟数据进行演示。首先,导入宏循环起始的数据集,并计算共现频次C_(j,k);然后,导出为out数据集,进一步合并数据集,形成最终的共现矩阵。结论SAS宏程序计算标准中关系型数据共现矩阵具有灵活高效的优势,可用于社会网络分析和互动演进规律总结。

心理韧性、父母情感支持与学生主观幸福感间的关系——基于PISA2018中国四省市数据分析

提升学生的幸福感是实现人的全面发展的必然要求,也是教育事业的重大使命。学生身心等非智力因素的发展在提高教育质量,全面推进素质教育等方面越来越受到重视。基于PISA2018中国四省市12058名中学生的调查数据,发现心理韧性、父母情感支持与学生主观幸福感均呈现正相关性;父母情感支持与心理韧性对学生主观幸福感有显著正向的线性影响,同时父母情感支持通过心理韧性的中介作用影响学生的主观幸福感。由此,提出了在言语鼓励、创造积极性情境、增强亲子互动方面父母应当给予学生情感支持以及从学校、教师与学生自身三个层面提升学生心理韧性的建议。

预热温度对SA387焊后残余应力和力学性能的影响

为了研究预热温度对SA387材料焊后力学性能和残余应力的影响,采用4种不同的预热温度分别焊接试板。对焊接完毕后的试板进行残余应力测定,发现随着预热温度的升高,焊接残余应力呈现先降低后升高的情况;通过力学性能测试和显微组织观察,发现预热温度对材料力学性能有很大影响,材料的抗拉强度和屈服强度随预热温度的升高而降低,韧性随着预热温度的升高先降低后升高。综合焊接残余应力和材料力学性能的测试结果,建议SA387 Gr11 Cl2的预热温度选择265~290℃。

多子阵SAS方位空变运动补偿子孔径算法

为解决方位空变的侧摆和偏航误差存在情形下合成孔径声纳的快速运动补偿与成像问题,提出一种多子阵合成孔径声纳方位空变运动补偿子孔径算法。首先,建立运动误差存在情形下的双程距离历程模型,并利用泰勒级数展开对双根号形式距离历程进行近似;然后,利用子孔径运动补偿和单基等效处理,将含有方位空变的侧摆和偏航误差的多子阵回波数据转换为理想的单阵回波数据;最后,利用经典的单阵频域逐线成像算法,实现快速运动补偿和高分辨成像。仿真实验与实测数据成像结果均验证了所提算法的有效性。

基于SA-Apriori的道路事故风险评估研究

道路事故风险评估是提高道路安全水平的有效方式。为实现在道路网络特征视角下的道路事故风险评估,融合线段分析与频繁集算法,提出SA-Apriori的特征提取算法,进而构建SA-Apriori道路事故风险评估模型,以重庆市大渡口区作为分析对象,建立以道路网络特征变量为指标的道路事故风险等级划分体系,评估其道路事故风险水平,结合商业点位分布情况对该评估方法的有效性进行验证。并针对大渡口区道路事故风险情况,提出道路事故风险的管理对策与建议。结果表明:该道路事故风险评估方法具有较高的可靠性,可为基于路网特征的道路事故风险评估提供参考。

认知行为干预在冠心病临床护理中的应用效果及对SAS、SDS评分的影响

目的:研究冠心病患者实施认知行为干预后的成效。方法:将聊城市第二人民医院2022年10月至2023年10月收治的80例冠心病患者作为研究对象,采用随机数字表法随机分成试验组和对照组,各40例。试验组受试者实施认知行为干预,对照组受试者实施常规护理。对干预后两组患者的护理满意度、抑郁焦虑评分、生活质量评分等观察指标进行评价。结果:干预后,试验组护理满意度高于对照组(P<0.05);干预后,试验组抑郁焦虑评分低于对照组(P<0.05);干预后,试验组生活质量评分高于对照组(P<0.05)。结论:对于冠心病患者展开认知行为干预的成效显著。该护理能够将患者的护理满意度、生活质量提升,使其心理状态得以改善。

分位数回归模型在公共卫生领域中的应用及SAS实现

目的探讨分位数回归模型在公共卫生领域中应用及SAS实现,为该方法推广使用提供参考。方法在介绍分位数回归模型基本概念基础上,介绍了分位数回归模型应用情景与优势,并以血清克拉拉细胞蛋白水平和第一秒用力呼气容积/用力肺活量(FEV1/FVC)变化数据为例,与简单线性回归模型相比,探讨分位数回归模型应用及SAS实现过程。结果分位数回归模型不仅可以分析血清克拉拉细胞蛋白水平对FEV1/FVC均值的影响,还可以分析血清克拉拉细胞蛋白水平对FEV1/FVC不同分位数的影响,得到更全面的信息,且通过统计软件SAS可方便实现。结论分位数回归模型可弥补简单线性回归模型仅关注应变量均值特征而不能分析其完整分布特征的不足,SAS软件提供了相对成熟的分析语句,值得推广。

早期心理干预对乳腺癌患者术后SAS、SDS及SIS评分的影响

目的探讨早期心理干预对乳腺癌患者术后SAS(焦虑自量量表)、SDS(抑郁自量量表)及SIS评分(社会影响量表)的影响。方法应用奇偶法随机将150例乳腺癌患者分为对照组和研究组,对照组行常规护理,研究组在其基础上实施早期心理干预,评估两组患者术后心理情况、病耻感、生活质量及护理满意度。结果研究组患者SAS评分、SDS评分及SIS评分均低于对照组(P<0.05);研究组患者社会功能、生理功能、躯体能力及活力均高于对照组(P<0.05);研究组患者护理满意度(96.00%)高于对照组(84.00%)(P<0.05)。结论在乳腺癌患者治疗中实施早期心理护理干预有助于稳定患者情绪、减少病耻感,生活质量与满意度更高,值得临床推广。

基于GRA-GASA-SVM的煤层瓦斯含量预测方法研究

为提升煤层瓦斯含量预测精度,提出一种采用遗传模拟退火算法混合优化支持向量机(SVM)参数的瓦斯含量预测模型(GRA-GASA-SVM模型)。该模型将GA和SA整合为遗传模拟退火算法协同优化SVM的参数,以解决传统网格寻优算法取值范围无法确定和单一智能算法优化程度有限等问题。利用灰色关联分析(GRA)压缩数据集维度,建立瓦斯含量预测参数体系并作为GASA-SVM的输入数据集。结果表明:SVM模型、GA-SVM模型和GASA-SVM模型10折交叉验证瓦斯含量预测总平均相对误差分别为15.98%、13.55%和10.58%。相比SVM模型和GA-SVM模型,GASA-SVM模型预测稳定性更优、预测精准度更高且对新样本泛化能力更强。

优质护理在急性肠胃炎患者中的护理效果观察及SDS、SAS评分影响分析

分析急性肠胃炎患者接受优质护理对SAS评分、SDS评分及护理效果影响。方法 本院急性肠胃炎患者抽选100例,就诊时间2021年1月-2022年12月,电脑抽签法分组。优质护理纳入研究组,基础护理纳入对照组。结果 研究组急性肠胃炎症状改善时间均较对照组短(P<0.05);研究组SAS评分、SDS评分均较对照组低(P<0.05);研究组生存质量分数均较对照组高(P<0.05);研究组护理满意度较对照组高(P<0.05)。结论 优质护理用于急性肠胃炎护理中,可稳定急性肠胃炎患者情绪、缩减急性肠胃炎症状持续时间、优化急性肠胃炎患者生存质量,值得推广应用。

结合机器学习的SA湍流模型闭合系数修正

将修正Morris分类筛选法与极端梯度提升(extreme gradient boosting,XGBoost)相结合,在计算流体动力学(computational fluid dynamics,CFD)数据驱动下,用于SA(Spalart-Allmaras)湍流模型闭合系数的修正.利用分类筛选法有效缩小闭合系数研究范围,同时依据XGBoost方法在小规模数据集下取得精度较高的拟合模型,有效提升系数修正效率.在三维DLR-F6-WB构型下进行了数值实验,实验结果显示利用本方法能够在三维复杂模型上基于小样本数据进行系数修正,修正后的升阻力系数计算精度得到了显著提升.

不同浓度外源SA对“赤霞珠”果实品质的影响

以8年生酿酒葡萄品种“赤霞珠”为试验材料,在葡萄转色期7月下旬到8月上旬喷施SA,探究在果实发育中SA对果实品质的影响,为改善酿酒葡萄品质提供参考。结果表明,对葡萄叶面喷施SA,整个生长周期“赤霞珠”百粒重无显著差异,葡萄进入转色期后,各SA处理下葡萄果实中糖类物质均逐渐增加,有机酸含量逐渐减少,采收期时,糖类物质含量达到最高,有机酸含量达到最低。采收期(花后110 d),5 mmol/L SA处理可以明显降低葡萄中可滴定酸含量,3 mmol/L SA处理可以显著提高葡萄果实中糖类物质和酚类物质含量。3 mmol/L SA处理有利于“赤霞珠”葡萄果实品质的改善。