Kudoa is a myxozoan that causes myoliquefaction in marine fishes.Most of species only affect fish,but a K.septempunctata outbreak was reported in 358 people.Although many species of Kudoa are known,none was described ...Kudoa is a myxozoan that causes myoliquefaction in marine fishes.Most of species only affect fish,but a K.septempunctata outbreak was reported in 358 people.Although many species of Kudoa are known,none was described in Brachyplatystoma filamentosum,Brachyplatystoma rousseauxii,Mugil curema,Plagioscion squamosissimus or Oxydoras niger until now.Due to the economic cost of eliminating seafood presenting myxozoan lesions,this study aimed to describe lesions found at necropsy and histopathology,as well as to detect this myxozoan by molecular techniques.For this purpose,were sampled 85 fish of the following species:Brachyplatystoma filamentosum,Brachyplatystoma rousseauxii,Mugil curema,Plagioscion squamosissimus,and Oxydoras niger from Colares and Vigia,Pará,Brazil.Necropsies were carried out to describe lesions and molecular techniques(PCR and sequencing)were applied for identification.Although muscle lesions were not observed at necropsy,histopathology revealed bacterial colonies,coagulative necrosis,dystrophic calcification,eosinophils,hemorrhage,parasitic pseudocysts,protozoan,and vacuolization.After sequencing,K.shiomitsui(GENBANK:LC128646)was identified as the most similar causative agent of fishes infection,but due to phylogenetic results and identity we suggest that the myxozoan found could be a new specie.Also,high parasitism of this myxozoan was observed in fishes sampled,i.e.,90%in Colares and 100%in Vigia.展开更多
基金The authors thank the City Council of Colares for logistical support,the Universidade Federal Rural da Amazônia(UFRA)and the Universidade Estadual Paulista(UNESP)the Brazilian Federal Agency for the support and evaluation of graduate education(Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-CAPES)the New Frontiers National Program of Academic Cooperation(Edictal PROCAD-NF,No 21/2009)for financial support.
文摘Kudoa is a myxozoan that causes myoliquefaction in marine fishes.Most of species only affect fish,but a K.septempunctata outbreak was reported in 358 people.Although many species of Kudoa are known,none was described in Brachyplatystoma filamentosum,Brachyplatystoma rousseauxii,Mugil curema,Plagioscion squamosissimus or Oxydoras niger until now.Due to the economic cost of eliminating seafood presenting myxozoan lesions,this study aimed to describe lesions found at necropsy and histopathology,as well as to detect this myxozoan by molecular techniques.For this purpose,were sampled 85 fish of the following species:Brachyplatystoma filamentosum,Brachyplatystoma rousseauxii,Mugil curema,Plagioscion squamosissimus,and Oxydoras niger from Colares and Vigia,Pará,Brazil.Necropsies were carried out to describe lesions and molecular techniques(PCR and sequencing)were applied for identification.Although muscle lesions were not observed at necropsy,histopathology revealed bacterial colonies,coagulative necrosis,dystrophic calcification,eosinophils,hemorrhage,parasitic pseudocysts,protozoan,and vacuolization.After sequencing,K.shiomitsui(GENBANK:LC128646)was identified as the most similar causative agent of fishes infection,but due to phylogenetic results and identity we suggest that the myxozoan found could be a new specie.Also,high parasitism of this myxozoan was observed in fishes sampled,i.e.,90%in Colares and 100%in Vigia.
