Network pharmacology-based strategy for predicting therapy targets of Sanqi and Huangjing in diabetes mellitus

BACKGROUND A comprehensive literature search shows that Sanqi and Huangjing(SQHJ)can improve diabetes treatment in vivo and in vitro,respectively.However,the combined effects of SQHJ on diabetes mellitus(DM)are still unclear.AIM To explore the potential mechanism of Panax notoginseng(Sanqi in Chinese)and Polygonati Rhizoma(Huangjing in Chinese)for the treatment of DM using network pharmacology.METHODS The active components of SQHJ and targets were predicted and screened by network pharmacology through oral bioavailability and drug-likeness filtration using the Traditional Chinese Medicine Systems Pharmacology Analysis Platform database.The potential targets for the treatment of DM were identified according to the DisGeNET database.A comparative analysis was performed to investigate the overlapping genes between active component targets and DM treatmentrelated targets.We constructed networks of the active component-target and target pathways of SQHJ using Cytoscape software and then analyzed the gene functions.Using the STRING database to perform an interaction analysis among overlapping genes and a topological analysis,the interactions between potential targets were identified.Gene Ontology(GO)function analyses and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were conducted in DAVID.RESULTS We screened 18 active components from 157 SQHJ components,187 potential targets for active components and 115 overlapping genes for active components and DM.The network pharmacology analysis revealed that quercetin,beta-sitosterol,baicalein,etc.were the major active components.The mechanism underlying the SQHJ intervention effects in DM may involve nine core targets(TP53,AKT1,CASP3,TNF,interleukin-6,PTGS2,MMP9,JUN,and MAPK1).The screening and enrichment analysis revealed that the treatment of DM using SQHJ primarily involved 16 GO enriched terms and 13 related pathways.CONCLUSION SQHJ treatment for DM targets TP53,AKT1,CASP3,and TNF and participates in pathways in leishmaniasis and cancer.

三七药对的研究进展

三七作为临床常用中药,在临床应用中有多种药对形式。围绕近年来基于网络药理学的三七药对、传统三七药对及三七药对配伍前后化学成分变化的实验研究进行归纳和总结,系统阐释三七药对在临床应用的作用机制和科学内涵,为进一步研究三七药对使用规律、配伍理论、临床应用等方面提供新的思路。

三七活骨丸联合体外冲击波对气滞血瘀型早期股骨头缺血性坏死患者的影响

目的 观察三七活骨丸联合体外冲击波(ESW)对气滞血瘀型早期股骨头缺血性坏死的影响。方法 选取2021年4月至2022年10月黑龙江中医药大学附属第二医院收诊的72例气滞血瘀型早期股骨头缺血性坏死患者,采用随机数字表法将其分为对照组和观察组,各36例。对照组采用ESW治疗,观察组在对照组的基础上口服三七活骨丸,两组均治疗4周。观察治疗前及治疗2、4周后两组视觉模拟评分法(VAS)评分、髋关节Harris评分、中医证候评分,并进行疗效评价。比较两组治疗前后血清白细胞介素(IL)-1β、IL-6、肿瘤坏死因子-α(TNF-α)、骨保护素(OPG)水平。结果 对照组有4例患者在治疗期间服用其他药物,故剔除。观察组临床疗效优于对照组(P<0.05)。两组治疗2、4周后VAS、中医证候评分均低于治疗前,髋关节Harris评分高于治疗前,两组治疗4周后VAS、中医证候评分均低于治疗2周后,髋关节Harris评分高于治疗2周后(P<0.05)。治疗2、4周后,观察组髋关节Harris评分高于同期对照组,中医证候评分低于同期对照组(P<0.05)。治疗4周后,观察组VAS评分低于同期对照组(P<0.05)。治疗4周后,两组血清IL-1、IL-6、TNF-α水平低于治疗前,血清OPG水平高于治疗前,且观察组血清IL-1、IL-6、TNF-α水平低于对照组,血清OPG水平高于对照组(P<0.05)。结论 三七活骨丸联合ESW对于改善气滞血瘀型早期股骨头缺血性坏死患者的症状疗效良好,适合临床应用。

三七对慢性肾衰竭大鼠Sortilin蛋白、Toll样受体及血管钙化的影响

目的 以Sortilin为切入点,探究慢性肾衰竭(CRF)血管钙化的机制,并探讨三七干预的影响,以期为临床降低CRF心血管事件提供有效的方法。方法 将36只雄性SD大鼠随机分为正常组、模型组、三七低、中、高剂量组、骨化三醇组,每组6只。采用腺嘌呤联合高磷饲料喂养复制CRF血管钙化大鼠模型。检测主动脉钙盐沉积,大鼠血清Scr、BUN、TG、TC、P、Ca,主动脉TLRs、Sortilin蛋白及炎症因子水平。结果 模型组大鼠肾纤维化明显,肾间质许多腺嘌呤结晶;大量钙盐沉积。各治疗组肾纤维化、钙盐沉积均有不同程度的减轻。与正常组比较,模型组大鼠血清BUN、Cr、P、TG、TC、IFN-γ、IL-6、IL-10、IL-17A含量明显升高(P <0.01),Ca含量明显降低(P <0.01)。与模型组比较,三七中、高剂量组和骨化三醇组大鼠血清BUN、Cr、P、TG、TC、IFN-γ、IL-6、IL-10、IL-17A含量明显降低(P <0.01),Ca含量明显升高(P <0.05或P <0.01)。与正常组比较,模型组大鼠主动脉组织BMP2、RUNX2、Sortilin、TLR7、TLR9蛋白的表达显著升高(P <0.01),SM22α蛋白的表达显著下降(P <0.01)。与模型组相比,三七低、中、高剂量组和骨化三醇组BMP2、RUNX2、Sortilin、TLR7、TLR9蛋白表达下降显著(P <0.01),SM22α蛋白表达显著上调(P <0.05或P <0.01),三七高剂量组和骨化三醇组效果更显著。结论 三七可改善CRF血管钙化模型大鼠的肾纤维化、血管钙化,其机制可能与调控Sortilin、TLRs的生物学功能有关。

基于网络药理学和分子对接技术探讨三七-淫羊藿治疗股骨头坏死的作用机制

目的运用网络药理学和分子对接技术探究三七-淫羊藿治疗股骨头坏死(ONFH)的作用机制。方法通过中药系统药理学数据库与分析平台筛选三七-淫羊藿的活性成分及其作用靶点,利用GeneCards®数据库、OMIM®数据库筛选ONFH的相关靶点,取交集后获得三七-淫羊藿治疗ONFH的潜在作用靶点。通过Cytoscape软件构建药物-活性成分-作用靶点网络,筛选关键活性成分。通过STRING数据库和Cytoscape软件构建蛋白-蛋白相互作用网络,筛选核心靶点。使用DAVID数据库对潜在作用靶点进行功能富集分析和通路富集分析。最后,对关键活性成分与核心靶点蛋白进行分子对接验证。结果最终获得三七-淫羊藿活性成分30个及其作用靶点214个,ONFH的相关靶点2112个。取交集后得到三七-淫羊藿治疗ONFH的潜在作用靶点131个。槲皮素、木犀草素、山柰酚、脱水淫羊藿素为三七-淫羊藿治疗ONFH的关键活性成分,蛋白激酶B1、血管内皮生长因子A、原癌基因c-Jun、肿瘤蛋白p53、白细胞介素1β为三七-淫羊藿治疗ONFH的核心靶点。三七-淫羊藿治疗ONFH的潜在作用靶点主要涉及对脂多糖的反应、对外来刺激的反应、细胞缺氧反应等生物过程,以及白细胞介素17信号通路、丝裂原活化蛋白激酶信号通路、糖尿病并发症中的晚期糖基化终末产物-晚期糖基化终末产物受体信号通路、磷脂酰肌醇3-激酶/蛋白激酶B信号通路等信号通路。分子对接结果显示,关键活性成分与核心靶点蛋白均具有较好的结合活性(结合能≤-5 kcal/mol)。结论三七-淫羊藿治疗ONFH的作用机制可能与调控免疫-炎症反应、调控破骨细胞与成骨细胞的凋亡和分化、改善血运等有关。

铍针结合三七散外敷治疗肱二头肌长头腱鞘炎疗效及对患者肌骨超声参数、血清炎症因子、肩关节功能的影响

目的:探讨铍针结合三七散外敷治疗肱二头肌长头腱鞘炎效果及对患者肌骨超声参数、血清炎症因子、肩关节功能的影响。方法:80例肱二头肌长头腱鞘炎患者随机分为对照组与观察组,各40例。对照组采用放射式冲击波治疗,观察组则实施铍针结合三七散外敷治疗,均连续治疗4周。比较两组临床疗效,治疗前后两组患者疼痛情况[视觉模拟疼痛评分(VAS)、压痛阈值(PPTs)]、肩关节功能[Constant-Murley肩关节功能活动评分量表(CMS)]、肌骨超声参数(肌腱厚度、腱鞘厚度、积液最大径)、血清炎症因子[肿瘤坏死因子-α(TNF-α)、C反应蛋白(CRP)、白细胞介素-6(IL-6)]的变化。结果:观察组患者临床疗效87.50%,明显高于对照组的67.50%(P<0.05);治疗后,观察组VAS评分低于对照组,观察组PPTs值高于对照组(P<0.05);观察组肌力、疼痛、肩关节活动度及功能评分均高于对照组(P<0.05);观察组肌腱厚度、腱鞘厚度、积液水平均低于对照组(P<0.05);观察组血清TNF-α、CRP、IL-6水平均低于对照组(P<0.05)。结论:铍针结合三七散外敷治疗肱二头肌长头腱鞘炎可提高临床效果,减轻患者疼痛,提高疼痛阈值,同时可降低患者血清炎症因子水平,改善肌骨超声参数和肩关节功能。

三七粉联合康妇凝胶治疗慢性宫颈炎伴HPV持续阳性临床观察

目的探讨三七粉联合康妇凝胶治疗慢性宫颈炎伴人乳头瘤病毒(HPV)持续阳性患者的临床效果。方法选取2018年6月—2020年12月于南昌市洪都中医院诊断为慢性宫颈炎伴HPV持续阳性的患者110例,采取随机数字表法将所有患者分为对照组(50例)和观察组(60例)。给予对照组重组人干扰素α2b栓治疗,给予观察组康妇凝胶联合三七粉治疗。比较治疗后两组的总有效率、HPV转阴率及血清炎症因子水平。结果治疗后观察组的总有效率为93.33%(56/60),高于对照组的82.00%(41/50)(P<0.05);治疗后观察组的HPV转阴率为85.00%(51/60),高于对照组74.00%(37/50)(P<0.05);治疗后观察组患者的肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)及白细胞介素-1β(IL-1β)炎症因子水平均低于对照组(P<0.05)。结论三七粉联合康妇凝胶治疗慢性宫颈炎伴HPV持续阳性的临床效果显著,能有效提高临床总有效率、提高患者的HPV转阴率、降低炎症因子水平,值得在临床治疗中推广应用。

三七治疗动脉粥样硬化药理机制研究进展

动脉粥样硬化(atherosclerosis,AS)是引发心脑血管系统疾病的主要原因之一,是动脉内膜异常沉积日久所致的慢性炎症性疾病,病机复杂、治疗周期长久,是临床需要重点解决的重点疾病之一。其病理机制涉及血管平滑肌细胞增殖、内皮损伤、血管内血栓形成、炎症反应、脂质代谢异常、氧化应激加剧、自噬稳态失衡等,而当前对中药三七有效成分是近年来研究的热点和重点,其富含多种皂苷类和非皂苷类有效化学成分,能够综合调理多方面的通路、分子机制,达到减轻炎症反应和内皮损伤,恢复正常脂质代谢、重新调整自噬稳态等的平衡,抑制血管内血栓形成、血管平滑肌细胞增殖,并最终达到预防和治疗AS的目的。鉴于三七在抗AS中的重要作用,该文就三七及其有效成分对AS保护作用的体内外等研究进行综述,为中医药新产品研究开发及临床应用辨证治疗用药提供参考和依据。

七味三七口服液辅助中药外洗治疗对髋关节置换手术患者术后患肢疼痛及肿胀情况的影响

目的 研究七味三七口服液辅助中药外洗治疗对髋关节置换手术患者术后患肢疼痛、肿胀情况的影响。方法 选取四川省骨科医院2021年6月至2022年6月行髋关节置换手术患者共120例。采用随机数字表法分为观察组和对照组,各60例。观察组患者术后予中药外洗、七味三七口服液治疗,对照组患者予术后常规治疗。比较2组中医证候积分、疼痛因子水平、患肢肿胀及不良反应发生情况。结果 治疗后,2组粗肿、胀痛、皮温升高、皮色改变中医证候积分均低于治疗前,且观察组均低于对照组(均P<0.05);2组β-内啡肽水平均高于治疗前,且观察组高于对照组,而2组前列腺素E2、5-羟色胺水平和视觉模拟量表评分均低于治疗前,且观察组均低于对照组(均P<0.05)。术后第3、7天,2组肿胀值均降低,且观察组均低于对照组[(0.63±0.21)cm比(0.75±0.24)cm、(0.47±0.12)cm比(0.66±0.17)cm](均P<0.05)。观察组不良反应发生率低于对照组(P<0.05)。结论 七味三七口服液辅助中药外洗可有效缓解髋关节置换手术患者术后患肢疼痛和肿胀,降低恶心、呕吐等不良反应发生的可能性,促进术后恢复。

试论三七补血

三七首载于《跌损妙方》,《本草纲目》云三七主治“吐血、咳血不止”“无名痈肿,疼痛不止”,后世本草多奉《本草纲目》所载为圭臬,言三七散瘀止血,消肿定痛,为活血化瘀之神药,直至《滇南本草》载三七“生用破血,炙用补血”,三七始有补血之说。现代研究发现熟三七的补血功效优于生三七,可以缓解血虚患者的临床症状,改善外周血象指标,通过保护造血器官、促进造血干/祖细胞的增殖、提高造血相关因子水平、改善造血微环境及代谢等作用发挥补血的作用。通过对三七的文献记载和现代研究的系统梳理,可为今后三七补血功效的临床应用及现代研究提供理论依据与参考。

参苓薏苡三七方联合止血生肌汤灌肠对脾虚湿热证溃疡性结肠炎患者的临床疗效

目的考察参苓薏苡三七方联合止血生肌汤灌肠对脾虚湿热证溃疡性结肠炎患者的临床疗效。方法114例患者随机分为对照组和观察组,每组57例,对照组给予止血生肌汤灌肠,观察组在对照组基础上加用参苓薏苡三七方,疗程4周。检测临床疗效、中医证候评分、Baron内镜评分、改良Mayo评分、VAS评分、肠道菌群(肠球菌、乳酸杆菌、肠杆菌、双歧杆菌)、相关因子(FCP、ESR、IL-10、TNF-α、TLR4、PGE2)、肠道屏障功能因子(L/M、DAO、D-乳酸)、安全性指标变化。结果观察组完全缓解率、总有效率高于对照组(P<0.05)。治疗后,2组中医证候评分、Baron内镜评分、改良Mayo评分、VAS评分、FCP、ESR、TNF-α、TLR4、PGE2、肠道屏障功能因子降低(P<0.05),IL-10升高(P<0.05),肠球菌、肠杆菌减少(P<0.05),乳酸杆菌、双歧杆菌增加(P<0.05),以观察组更明显(肠球菌除外)(P<0.05)。2组均未见明显不良反应。结论参苓薏苡三七方联合止血生肌汤灌肠可安全有效地调节脾虚湿热证溃疡性结肠炎患者肠道菌群,改善肠道屏障功能,提高镇痛、消炎效果。

基于网络药理学和分子对接探讨三七-骨碎补治疗股骨头坏死的作用机制

目的 基于网络药理学和分子对接探究“三七-骨碎补”药对治疗股骨头坏死的作用机制。方法 利用中药系统药理学数据库与分析平台(TCMSP)对“三七-骨碎补”药对的活性成分及成分靶点进行采集、筛选,通过CNKI、PubMed数据库对其进行补充,并利用UNIPORT数据库对靶点进行转换;通过GeneCards、TTD和OMIM数据库收集股骨头坏死的作用靶点,取得交集靶点后导入STRING数据库,生成PPI网络,并通过Cytoscape 3.9.0软件构建中药-活性成分-靶点网络;通过DAVID数据库对核心靶点进行GO功能富集分析与KEGG通路富集分析。结果 共得到“三七-骨碎补”药对的20个活性成分和229个潜在靶点,1386个股骨头坏死的靶点。GO富集分析主要包括炎症反应、凋亡过程、调节血管生成、与雌性激素响应等功能;KEGG富集分析主要包括脂质与动脉硬化、白细胞介素-17信号通路、类风湿性关节炎等信号通路。结论 “三七-骨碎补”药对治疗股骨头坏死具有成分多、靶点广、通路复杂的特点,可能通过IL6、TNF、TP53等靶点以及脂质代谢、白细胞介素-17等信号通路共同发挥治疗股骨头坏死的作用。

Effect of Sanqi Oral Liquid on the Expressions of CD4＋, CD8＋ and CD68＋Cells in 5/6 Nephrectomized Rats with Chronic Renal Failure~

Objective： To explore the mechanisms of Chinese herbal medicine Sanqi Oral Liquid , composed of Astragalus membranaceus and Panpax notoginseng, in alleviating renal injury by observing its effect on the expressions of CD4＋, CD8＋ and CD68＋ cells in 5/6 nephrectomized rats with chronic renal failure. Methods： A total of 102 SD rats were randomly divided into six groups： three treatment groups were administrated with high, medium and low dosage of Sanqi Oral Liquid respectively by gavage; a normal group, a 5/6 nephrectomized model group, and a group treated with coated aldehyde oxygenstarch were used as controls. Following oral administration of Sanqi Oral Liquid for 12 weeks, the general condition and renal pathological changes were observed, and the renal function, platelet count （PLT） and the expressions of CD4~, CD8＊ and CD68~ cells were determined for each group. Results： There were proliferation of mesangial matrix, renaltubularnecrosis and obvious tubulointerstitial fibrosis in the model group, and they were much milder in the treatment groups. Compared with the model group, the amounts of blood urea nitrogen （BUN）, serum creatinine （Scr） and PLT in the treatment groups decreased （P〈0.05 for all）; and in the group administrated of medium dosage of Sanqi Oral Liquid, the expression of CD4＋ cells was up-regulated and those of CD8＋ and CD68＋ cells were down-regulated （P〈0.05 for all）, leading to an increased ratio of CD4＋/CD8＋ （P〈0.01）. Conclusion： Sanqi Oral Liquid has a significant effect on regulating lymphocyte subsets, reducing the infiltration of macrophages in renal tissues and alleviating tubulointerstitial fibrosis, and this may be one of mechanisms of Sanqi Oral Liquid in delaying the progression of chronic kidney diseases.

基于ICP-MS对三七血伤宁散中16种无机元素的分析及质量评价

采用电感耦合等离子体质谱(ICP-MS)对三七血伤宁散(SQXSNP)中无机元素组成进行分析并建立相关指纹图谱,为产品质量均一性和安全性评价提供参考。以HNO_(3)为消解溶剂,采用ICP-MS法定量测定三七血伤宁散中无机元素的含量,通过无机元素特征图谱,Pearson相关系数对结果进行分析;通过聚类分析结合主成分分析(PCA)对质量均一性进行评价。三七血伤宁散中无机元素以Mg、Fe、Al、Mn和Zn为主,且不同元素含量之间具有一定相关性。Cu和As这两种重金属元素的限量符合药典相关限度规定。不同批次的样品谱图均有相似的峰形。指纹图谱、聚类分析及主成分分析结果表明,三七血伤宁散中无机元素的质量均一性较好,主要特征元素为Li、Ga、Fe、Mg、V、Ni和Sr。本研究操作方法简便、分析速度快、准确度高,适用于三七血伤宁散中无机元素的测定,可为三七血伤宁散的质量评价和综合利用提供理论依据。

三七总皂苷对脑缺血再灌注后神经功能恢复的影响

目的探究三七总皂苷对脑缺血再灌注后神经功能恢复的影响。方法培养30只SPF级雄性SD大鼠,构建脑缺血再灌注模型。将大鼠分为假手术组、模型组和三七总皂苷组,每组10只大鼠。检测各组大鼠脑梗面积、脑含水量。Longa评分评价大鼠神经功能缺损情况。水迷宫实验检测大鼠神经功能。检测各组大鼠血清中氧化应激指标超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)、谷光甘肽(glutathione,GSH)含量和炎症因子指标肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-1β(interleukin-1β,IL-1β)、白细胞介素-10(interleukin-10,IL-10)含量。Tunel染色检测各组大鼠海马神经元细胞凋亡情况。结果和假手术组大鼠相比,模型组大鼠脑梗面积、脑含水量、神经功能缺损评分、学习潜伏期和记忆潜伏期显著升高,SOD、GSH、IL-10含量显著降低,MDA、TNF-α、IL-1β含量显著升高,海马神经元凋亡比例显著升高(P<0.05)。和模型组大鼠相比,三七总皂苷组大鼠脑梗面积、脑含水量、神经功能缺损评分、学习潜伏期和记忆潜伏期显著降低,SOD、GSH、IL-10含量显著升高,MDA、TNF-α、IL-1β含量显著降低,海马神经元凋亡比例显著降低(P<0.05)。结论三七总皂苷可能通过抑制大鼠炎症反应和氧化应激,减少海马神经元凋亡,促进脑缺血再灌注后神经功能恢复。

基于网络药理学探究三七叶对糖尿病大鼠的治疗机制

目的 利用网络药理学和动物实验,研究三七叶抗糖尿病的主要活性成分和潜在作用机制。方法 基于Pubchem、PharmMapper、OMIM等数据库筛选三七叶与糖尿病共同的潜在靶点之后,再利用STRING数据库和Cytoscape软件构建蛋白相互作用网络图,对其进行拓扑学分析和聚类分析获得核心靶点,并进行GO和KEGG富集分析筛选药物作用于疾病的可能机制。随后进行动物实验验证三七叶的抗糖尿病作用。结果 预测结果共获得三七叶与糖尿病的共同靶点50个,核心靶点有3个:ANXA5、CFB、GSTP1,共涉及生物过程986种,细胞组成15种,分子功能74种,信号通路101条。动物实验表明三七叶可降低糖尿病大鼠的血糖,减少与糖尿病肾病相关的p38 MAPK和TGF-β1 mRNA的表达,同时也可以调节氧化应激和血脂水平,减少动脉粥样硬化。结论 三七叶是通过多途径多靶点共同发挥抗糖尿病的作用,其作用机制可能与减少氧化应激、调节细胞因子、减少动脉粥样硬化和抗糖尿病肾病肾纤维化等有关。

Blood circulation activating effect of Sanqi(Radix Notoginseng) on venous thromboembolism rats

OBJECTIVE:To explore the blood circulation activating effect and mechanism of Sanqi(Radix Notoginseng)in vivo,using a venous thromboembolism(VTE)rat model.METHODS:We established the VTE rat model,and then intervened with low molecular weight hepa-rin(LMWH),as well as low,medium and high doses of Sanqi(Radix Notoginseng),to observe the blood circulation activating effect of Sanqi(Radix Notoginseng)on VTE rats.RESULTS:After the treatment with high concentrations of Sanqi(Radix Notoginseng),the pulmonary thromboembolism was alleviated,and the lower limb thrombosis was markedly improved.Moreover,the expression quantities of plasma activated partial thromboplastin time,prothrombin time and D-dimer,as well as endothelin,von Willebrand factor,and plasminogen activator inhibitor-1 in thrombosis segment tissues were markedly down-regulated;while those of nitric oxide and tissue-type plasminogen activator were up-regulated.After low and medium concentration Sanqi(Radix Notoginseng)treatment,no obvious improvement was observed in each index.Moreover,the high concentration Sanqi(Radix Notoginseng)showed comparable efficacy to the positive drug LMWH.CONCLUSION:This data suggests that high concentration of Sanqi(Radix Notoginseng)is effective in preventing and treating VTE.

Efficacy of Sanqi(Radix Notoginseng)in treating cerebral hemorrhage in rats with traumatic brain injury

OBJECTIVE:To evaluate the protective efficacy of Sanqi(Radix Notoginseng)on cerebral hemorrhage in a rat model of traumatic brain injury(TBI)by investigating plasminogen activator inhibitor-1(PAI-1),tissue-type plasminogen activator(t-PA),nuclear factor-κB(NF-κB,p-p65),nitric oxide(NO),endothelin(ET),cluster differentiation(CD61CD62),and coagulation.METHODS:The free-fall method was used to create a rat model of TBI.Forty-eight rats were randomly divided into six groups:the blank group,sham group,model group,low-dose Sanqi(Radix Notoginseng)group,middle-dose Sanqi(Radix Notoginseng)group,and high-dose Sanqi(Radix Notoginseng)group.At 24 h after the model was created,we investigated brain MRI,brain tissue morphology using HE staining,flow cytometry,and immunohistochemical changes.RESULTS:Cerebral hemorrhage was aggravated in TBI rats(observed in brain specimens,brain MRI,and brain tissue HE).Cerebral immunohistochemistry results demonstrated that the expression of t-PA,PAI-1 and p-p65 increased significantly in TBI rats,while t-PA/PAI-1 had a significant decrease.In addition,CD61CD62,D2D,and ET were significantly increased in TBI rats,and PT and APTT were significantly prolonged;in contrast,NO was significantly decreased.Sanqi(Radix Notoginseng)decreased cerebral hemorrhage in TBI rats(observed in brain MRI and brain tissue HE),and increased t-PA/PAI-1,CD61CD62 significantly.It also significantly decreased the expression of t-PA,PAI-1,and p-p65 in brain immunohistochemistry and significantly decreased PT,APTT,D2D,and ET.However,there were no differences in NO between the model group and the Sanqi(Radix Notoginseng)group.CONCLUSION:Sanqi(Radix Notoginseng)can decrease the expression of p-p65,increase t-PA/PAI-1,and stem traumatic intracranial hemorrhage in a TBI rat model.

Efficacy of Renshen Sanqi Chuanxiong formula for preventing vascular aging

OBJECTIVE:To evaluate the efficacy of Renshen Sanqi Chuanxiong formula(RSCF)for preventing vascular aging,and to investigate the possible molecular mechanism underlying the actions of RSCF.METHODS:Potentially active components and their relatively direct targets were identified by combining drug-likeness(DL)screening using a target identification process.Vascular aging-associated targets for RSCF were obtained by selecting common genes not only from potential targets but also from human vascular aging-associated genes.Cytoscape 3.2.1 software was employed to visualize the complex compound-target and target-function networks.Biological process and molecular function were assessed,and the Kyoto Encyclopedia of Genes and Genomes and pathway enrichment analyses were performed using Clue GO.Pathways directly associated with vascular aging were integrated into a"vascular aging-related"pathway.RESULTS:Altogether,122 potentially active components of RSCF were identified through DL screening,and their corresponding 692 direct targets were retrieved via target prediction and identification.We identified 49 vascular aging-associated targets for RSCF by overlapping the 692 potential targets with 146 human vascular aging-associated genes.The results from the compound-target network indicated that most components acted on common targets and displayed synergistic action,which showed that the magnifying effects of RSCF were based on these common targets.The target-function network revealed that each target was involved in multiple function modules,suggesting that RSCF was multi-functional during treatment of vascular aging.The results of the Clue GO analysis indicated that most of the targets were associated with the hypoxia-inducible factor 1(HIF-1)signaling pathway.The results from the pathway analysis also indicated that an integrative vascular aging-related pathway mainly included an angiogenesis regulation module,cell-survival module,and oxidative stress-resistance module.CONCLUSION:Our results suggested that many components act synergistically on common targets to delay vascular aging,and each target is involved in multiple functional modules.The Clue GO analysis indicated that most of the targets were connected to the HIF-1 signaling pathway,FOXO signaling pathway,and thyroid hormone signaling pathway.

加味复方三七颗粒治疗稳定型心绞痛肾虚血瘀证临床观察

目的:观察加味复方三七颗粒治疗稳定型心绞痛肾虚血瘀证临床疗效。方法:纳入稳定型心绞痛肾虚血瘀证患者64例,随机分为两组各32例。对照组予常规西医治疗,治疗组在对照组基础上予加味复方三七颗粒,两组均治疗4周。比较两组治疗后中医证候积分、中医证候疗效、心绞痛发作时间及频率、心电图疗效、硝酸甘油减停率及血液流变学指标。结果:治疗组中医证候疗效为90.63%,优于对照组的75.00%(P<0.05);治疗组心电图疗效为87.50%,优于对照组的68.75%(P<0.05)。两组中医证候积分、心绞痛发作频率及时间、血液流变学相关指标水平均不同程度下降,治疗组较对照组下降更明显(P<0.05);治疗组硝酸甘油停减率为90.63%,优于对照组的75.00%(P<0.05);两组均无不良反应发生。结论:加味复方三七颗粒治疗稳定型心绞痛肾虚血瘀证患者临床效果显著,安全有效。