GNSS拒止时基于并行CNN-BiLSTM回归和残差补偿的UAV导航误差校正方法

全球导航卫星系统(GNSS)拒止时,GNSS/惯性导航系统(INS)组合导航系统的性能严重下降,导致无人机集群导航误差快速发散.目前,利用神经网络预测位置与速度代替GNSS导航信息可校正无人机INS误差,但该方法仍存在定位误差较高且在轨迹突变时预测精度急剧下降的问题.因此,提出了一种基于卷积-双向长短时记忆网络联合残差补偿的位置与速度预测方法,用于提高位置与速度预测精度.首先,针对GNSS拒止后GNSS/INS组合导航系统定位误差较高的问题,提出卷积神经网络(CNN)与双向长短时记忆网络(BiLSTM)的融合模型,该模型可建立惯性测量单元(IMU)动力学测量数据与GNSS导航信息之间的关系,实现较准确的位置和速度预测.其次,针对轨迹突变时预测效果急剧下降的问题,提出并行CNNBiLSTM回归架构,在预测位置与速度的同时,挖掘IMU动力学测量数据、预测值与预测残差之间的关系,预测并补偿预测残差,增强模型在轨迹突变时的预测精度.仿真结果表明,所提模型在预测准确性、有效性和稳定性方面都优于CNN-LSTM、LSTM网络模型.

基于MSW-LSTM的卫星功率负荷预测方法

文章提出一种基于多尺度小波变换的长短期记忆神经网络(Multi-scale Wavelet-Long Short-term Memory,MSW-LSTM)卫星功率负荷预测方法,利用多尺度小波变换的时频特性将卫星功率负荷数据进行平稳化处理,结合LSTM算法针对多尺度小波分解并降噪后的各尺度功率负荷序列进行预测,并对预测得到的各序列数据进行重构得到时域功率负荷数据,从而提高卫星功率负荷预测的精度。通过某在轨卫星真实功率负荷数据进行分析,仿真结果表明本文提出的算法可以有效地提高功率负荷预测的精度,且针对不同轨道的卫星功率负荷预测有较高的普适性和鲁棒性。

形状回复力对抑制破骨细胞形成的调控作用

破骨细胞是骨吸收的主要功能细胞,具有力学敏感性。本研究旨在利用形状记忆聚合物(SMPs)的形状回复力原位调控破骨细胞的形成。首先将纯聚乳酸(PLA)、不同质量比(7∶3、5∶5、3∶7)的PLA和聚己内酯(PCL)通过热致相分离法(TIPS)制备PLA/PCL 3D支架,并表征其形貌、结构、热性能;然后,将筛选的PLA/PCL(5∶5)支架在45℃下压缩塑形(压缩比分别为0、50%、75%)和4℃下冷却固定,并表征形状记忆性能;最后,通过基于RAW264.7巨噬细胞的破骨分化实验验证了形状回复力对抑制破骨细胞形成的调控作用。结果表明:PLA/PCL(5∶5)材料体系的形状回复温度接近人体体温,适合制备3D支架;形状回复力与塑形应变相关,压缩比75%组的支架产生的形状回复力为50%组的1.7倍;形状回复力可抑制巨噬细胞的融合分化及相关功能表达,对破骨细胞形成的抑制作用与回复力大小有关。

骨成型蛋白-4参与糖尿病心肌病“高糖记忆”现象的机制

目的 研究骨成型蛋白-4(BMP4)在糖尿病心肌病(DCM)“高糖记忆”中的作用。方法 体外实验:原代培养C57BL/6小鼠乳鼠心肌细胞,随机分为正常浓度葡萄糖组(Normal glucose,NG)、高浓度葡萄糖组(High glucose,HG)、“高糖记忆”组(HGNG),分别采用NG(5.6 mM)、HG(33 mM)、HG培养4 d+NG培养4 d,每组均培养8 d。培养结束后采用Western Blotting(WB)法检测凋亡蛋白Caspase-3及BMP4在DCM心肌细胞中的表达情况。以BMPs受体抑制剂DMH1作用于DCM“高糖记忆”模型细胞,观察Caspase-3及BMP4表达变化。体内实验:注射链脲佐菌素(50 mg/kg)连续5 d建立糖尿病C57BL/6小鼠模型,每日给予二甲双胍(Metformin,MET,320 mg/kg)灌胃治疗,连续给药8周,血糖仪检测血糖、WB法检测caspase-3及BMP4在小鼠心脏组织中表达情况。结果 在HG组中,HG可明显诱导心肌细胞BMP4蛋白表达,与NG组比较差异显著(P<0.01);DCM“高糖记忆”模型(HGNG组)心肌细胞BMP4蛋白表达显著升高,与NG组比较差异显著(P<0.01),与HG组比较无统计学差异(P>0.05)。DMH1可明显抑制DCM“高糖记忆”模型细胞中BMP4及Caspase-3蛋白表达(P<0.01)。MET可降低糖尿病小鼠血糖及心体比指数,抑制Caspase-3蛋白表达(P<0.01),但并不抑制BMP4蛋白表达。结论 BMP4是产生DCM“高糖记忆”现象的机制之一,BMPs受体抑制剂DMH1改善DCM“高糖记忆”引起的心肌细胞损伤。

基于长短时记忆神经网络的Multi-GNSS卫星钟差建模预报

针对卫星钟差预报中二次多项式模型存在易受噪声干扰、预报精度不高的问题,构建一种基于长短时记忆神经网络的multi-GNSS卫星钟差预报模型,并分析不同卫星系统、不同钟类型基于不同建模方案的模型精度。为验证该模型的有效性和可行性,利用LSTM模型、QP模型、QP-LSTM模型分别基于12 h和24 h钟差序列进行建模,预报1 h、3 h、6 h、12 h钟差。结果表明,LSTM模型建模24 h、预报1 h精度最高。multi-GNSS卫星钟差LSTM预报模型中Galileo系统精度最高,其次为BDS-2系统和GPS系统,GLONASS系统精度最低,精度分别为0.018 ns、0.069 ns、0.133 ns、0.242 ns。不同原子钟预报精度不同,氢原子钟预报精度优于铷原子钟、铯原子钟。LSTM神经网络模型预报精度相较于QP-LSTM模型提升27%,相较于QP模型提升36%。

联合SSA与BiLSTM的北斗卫星钟差预报算法

针对现有的卫星钟差预报模型难以捕捉其非线性特性的问题,提出了一种联合麻雀搜索算法(SSA)与双向长短期记忆神经网络(BiLSTM)的北斗卫星钟差预报算法。将BiLSTM应用于钟差预报中,并引入SSA进行网络超参数选择,能够更好地捕捉钟差数据中的特征关系,提高模型预报的准确性。利用德国地球科学研究中心提供的北斗三号精密卫星钟差数据,进行了1 h、3 h、6 h、12 h、24 h和48 h的钟差预报实验;与常用模型从卫星轨道类型和模型普适性方面,进行了单天与多天的预报对比分析。结果表明,相对于多项式模型、小波神经网络、长短期记忆神经网络模型和BiLSTM模型,所提算法的钟差预报平均精度分别提升了75.12%、67.44%、75.18%和48.65%。

多感官互动体验在图书馆展览中的应用——以国家图书馆“甲骨文记忆”展为例

多感官互动体验是博物馆展览中应用较多的策展方式,在图书馆展览服务中亦具有较高的可行性。国家图书馆“甲骨文记忆”展采用多感官设计理念,在展览中设置了8个多感官互动体验项目,为观众带来全新的观展感受。文章采用问卷调查法及访谈法,获知观众在展览中的实际参观感受,以及在语言和行为上所表现出来的主观情感态度,以此分析多感官互动体验在图书馆展览中应用的实际效果及改进空间,从而为多感官互动体验在图书馆展览,尤其是典籍文献类展览中的广泛应用提供借鉴。

“两弹一星”精神与集体记忆叙事研究——以典型人物报道为例

集体记忆对于建构历史认同感与群体归属感有着重要作用,属于历史的记忆与属于当下的时代背景是互相依赖的,在当下的新闻报道中重现历史的记忆,可以唤醒受众内心深处的记忆,并赋予记忆以时代感。“两弹一星”精神,是中华民族宝贵的精神财富,也是中华民族集体记忆的重要组成部分。典型人物作为特殊的精神符号,是集体记忆的重要来源和支撑,相应地,典型人物报道也一直是我国新闻传播领域的重要实践方法,在传播主流思想价值、弘扬社会主义核心价值观、引领和激励全社会成员积极践行党的路线方针政策方面具有巨大的示范作用。主流媒体在“两弹一星”精神的典型人物报道中,通过突出表达彼时我国面临的复杂多变的国际形势与艰苦贫穷的国内现状,依托历史背景呈现了“两弹一星”精神集体记忆的时空缘起;通过运用典型人物语言符号、整合红色记忆文字符号、应用“两弹一星”数字符号、引用功勋人物称号符号等,增强了“两弹一星”精神集体记忆的形象表达,具有实现了唤醒记忆、弘扬精神,回望历史、聚焦当下,形成认同、指引未来的重要现实意义。

镍钛形状记忆合金骨植入物中环氧乙烷残留量测定方法

目的:建立适合医疗器械产品镍钛形状记忆合金弹力聚合器环氧乙烷残留量测定的顶空气相色谱法。方法:采用气相色谱法-氢火焰离子化检测器进行检测,色谱柱为HP-INNOWax,柱温80℃保持15 min,进样口温度200℃,检测器温度250℃,氢气流量35 mL/min,空气流量350 mL/min,平衡时间40 min。结果:环氧乙烷在1~10μg/mL线性良好,方法检出限0.01 mg/L,精密度RSD=0.01%,回收率在86.6%~96.6%。为保证结果的准确性,样品应立刻测定,剪样面积应尽可能小。结论:该方法操作简单,检出限低,可以用于镍钛形状记忆合金骨植入物中环氧乙烷残留量的快速测定。

胸腔镜下镍钛形状记忆合金接骨板内固定对多发性肋骨骨折患者围手术期指标及肺通气功能的影响

目的:探讨胸腔镜下镍钛形状记忆合金接骨板内固定对多发性肋骨骨折患者围手术期指标及肺通气功能的影响。方法:选取2022年1月—2024年1月枣庄市峄城区人民医院收治的64例多发性肋骨骨折患者作为研究对象,随机分为研究组和对照组,各32例。研究组采取胸腔镜下镍钛形状记忆合金接骨板内固定治疗,对照组采取传统开胸手术内固定治疗,比较两组围手术期指标及肺通气功能。结果:研究组手术时间、术后引流时间和住院时间均短于对照组,研究组术中出血量少于对照组,差异均有统计学意义(P<0.05)。术后第1、3、7天,研究组肺活量、用力肺活量和第1秒用力呼气容积均高于对照组,差异均有统计学意义(P<0.05)。结论:胸腔镜下镍钛形状记忆合金接骨板内固定治疗多发性肋骨骨折,能有效改善患者围手术期指标,提高肺通气功能。

Navigation jamming signal recognition based on long short-term memory neural networks

This paper introduces the time-frequency analyzed long short-term memory(TF-LSTM) neural network method for jamming signal recognition over the Global Navigation Satellite System(GNSS) receiver. The method introduces the long shortterm memory(LSTM) neural network into the recognition algorithm and combines the time-frequency(TF) analysis for signal preprocessing. Five kinds of navigation jamming signals including white Gaussian noise(WGN), pulse jamming, sweep jamming, audio jamming, and spread spectrum jamming are used as input for training and recognition. Since the signal parameters and quantity are unknown in the actual scenario, this work builds a data set containing multiple kinds and parameters jamming to train the TF-LSTM. The performance of this method is evaluated by simulations and experiments. The method has higher recognition accuracy and better robustness than the existing methods, such as LSTM and the convolutional neural network(CNN).

Improvement of learning and memory abilities and motor function in rats with cerebral infarction by intracerebral transplantation of neuron-like cells derived from bone marrow stromal cells

BACKGROUND： Transplantation of fetal cell suspension or blocks of fetal tissue can ameliorate the nerve function after the injury or disease in the central nervous system, and it has been used to treat neurodegenerative disorders induced by Parkinson disease. OBJECTIVE： To observe the effects of the transplantation of neuron-like cells derived from bone marrow stromal cells （rMSCs） into the brain in restoring the dysfunctions of muscle strength and balance as well as learning and memory in rat models of cerebral infarction. DESIGN ： A randomized controlled experiment.SETTING ： Department of Pathophysiology, Zhongshan Medical College of Sun Yat-sen University.MATERIALS ： Twenty-four male SD rats （3-4 weeks of age, weighing 200-220 g） were used in this study （Certification number：2001A027）. METHODS： The experiments were carried out in Zhongshan Medical College of Sun Yat-sen University between December 2003 and December 2004. ① Twenty-four male SD rats randomized into three groups with 8 rats in each： experimental group, control group and sham-operated group. Rats in the experiment al group and control group were induced into models of middle cerebral artery occlusion （MCAO）. After in vitro cultured, purified and identified with digestion, the Fischer344 rMSCs were induced to differentiate by tanshinone IIA, which was locally injected into the striate cortex （18 area） of rats in the experimental group, and the rats in the control group were injected by L-DMEM basic culture media （without serum） of the same volume to the corresponding brain area. In the sham-operated group, only muscle and vessel of neck were separated. ② At 2 and 8 weeks after the transplantation, the rats were given the screen test, prehensile-traction test, balance beam test and Morris water-maze test. ③ The survival and distribution of the induced cells in corresponding brain area were observed with Nissl stained with toluidine blue and hematoxylin and eosin （HE） staining in the groups.MAIN OUTCOME MEASURES ： ① Results of the behavioral tests （time of the Morris water-maze test screen test, prehensile-traction test, balance beam test）; ② Survival and distribution of the induced cells.RESULTS： All the 24 rats were involved in the analysis of results. ① Two weeks after transplantation, rats with neuron-like cells grafts in the experimental group had significant improvement on their general muscle strength than those in the control group [screen test： （9.4±1.7）, （4.7±1.0） s, P 〈 0.01]; forelimb muscle strength [prehensile-traction test： （7.6±1.4）, （5.2±1.2） s, P 〈 0.01], ability to keep balance [balance beam test： （7.9±0.74）, （6.1±0.91） s, P 〈 0.01] and abilities of learning and memory [latency to find the platform： （35.8±5.9）, （117.5±11.6） s, P 〈 0.01; distance： （623.1±43.4）, （1 902.3±98.6） cm, P 〈 0.01] as compared with those in the control group. The functional performances in the experimental group at 8 weeks were better than those at two weeks, which were still obviously different from those in the sham-operated group （P 〈 0.05）. ② The HE and Nissl stained brain tissue section showed that there was nerve cell proliferation at the infarcted cortex in the experiment group, the density was higher than that in the control group, plenty of aggregative or scattered cells could be observed at the site where needle was inserted for transplantation, the cells migrated directively towards the area around them, the cerebral vascular walls were wrapped by plenty of cells; In the control group, most of the cortices were destroyed, karyopyknosis and necrosis of neurons were observed, normal nervous tissue structure disappeared induced by edema, only some nerve fibers and glial cells remained.CONCLUSION： The rMSCs transplantation can obviously enhance the motor function and the abilities of learning and memory in rat models of cerebral infarction.

Fabrication of Dex-Loaded Shape Memory Polymer Based Composite Nanofibers for Potential Bone Tissue Engineering

Biodegradable shape memory polymers( SMPs) are a class of intelligent materials with great potential for imparting biomaterial scaffolds multifunctionality in the field of tissue engineering and regenerative medicine.In this study,the biodegradable SMP poly( D,L-lactide-co-trimethylene carbonate)( PLMC) incorporated with the dexamethasone( Dex),which was a kind of synthetic bone-formation inducing factor,was fabricated into nanofibers via electrospinning.The morphology,constituent,thermal and mechanical properties of the produced Dex / PLMC composite nanofibers were characterized by scanning electron microscopy( SEM), Fourier transform infrared spectroscopy( FTIR), differential scanning calorimetry( DSC),and tensile testing,respectively.Then,ultrasound was employed as a remote stimulus to regulate the Dex releasing behavior from the composite nanofibers.It was found that the generated Dex /PLMC composite nanofibers had a uniform and smooth morphology with a diameter of ca.564 nm.Mechanical testing results showed that incorporation of the Dex gave rise to improved mechanical performance with the tensile strength,Young's modulus and strainat-break increased by 18.2%,20.0% and 64.4%,respectively.DSC data revealed that the glass transition temperature( Tg) of the composite nanofibers, i.e., the thermal transition temperature( Ttrans) for activating shape memory effect, was 39.7 ℃.Moreover, the release kinetics of the encapsulated Dex in the nanofibers could be manipulated by varying the acoustic power and insonation duration.These results suggested that the newly developed Dex / PLMC nanofibers could be a promising drug delivery system for applications in bone tissue engineering( BTE).

基于改进有效信噪比的混合自动重传请求算法

针对AOS系统中传统的混合自动重传请求算法在传输过程中难以同时兼顾数据传输的误码率和传输效益的问题,提出一种基于改进有效信噪比的混合自动重传请求算法。在基于有效信噪比的自动重传请求的基础上,采用长短期记忆网络和隐马尔科夫组成的混合模型预测有效信噪比的变化。根据不同时刻有效信噪比的变化关系动态调节汉明码的监督元数量,实现保证系统误码率的同时提升系统的吞吐率。仿真结果表明,该算法与传统II型混和自动重传请求算法和基于有效信噪比的混和自动重传请求算法相比,能够明显降低误码率,减少重传次数,提升系统的吞吐率。

腰椎椎体间镍钛记忆合金支架的生物力学分析

目的测试镍钛记忆合金支架的力学性能及用于腰椎间融合术的体外生物力学性能。方法通过力学测试机测试支架的力学性能,并在新鲜腰椎标本上模拟腰椎间融合术,对比分析镍钛记忆合金支架及其配套的骨粒-可吸收骨水泥混凝物浇注植骨方法、传统盒状融合器用于腰椎间融合术的生物力学性能。结果镍钛记忆合金支架最大抗压强度为(12964±962)N,记忆特性有效范围内最大形变为(4.68±0.03)mm,形状恢复率为99.86%。相比于正常腰椎模型,单独使用镍钛记忆合金支架植入模拟腰椎间融合术后手术节段稳定性在前屈、后伸、侧屈、旋转方向上均有增加,与采用传统盒状融合器相当(P>0.05)。而在复合使用配套设计的自体骨粒-可吸收骨水泥混凝物植骨后,手术节段活动度进一步降低(P<0.05),节段稳定性与附加椎弓根螺钉内固定组相当(P>0.05)。镍钛记忆合金支架植入后的抗拔出强度弱于传统盒状融合器(P<0.05),在配合使用自体骨粒-可吸收骨水泥混凝物植骨时的抗拔出强度显著强于传统盒状融合器(P<0.05)。结论本研究的镍钛记忆合金支架同时配套设计了相匹配的骨粒-骨水泥混凝物植骨方式,为腰椎间融合术的进一步优化发展提供了新思路。镍钛记忆合金支架具有良好的支撑及形变性能,用于腰椎间融合术时与传统盒状融合器具有生物力学等效性,在复合应用骨粒-可吸收骨水泥混凝物浇注植骨后能进一步提升稳定性和抗拔出强度。

LSTM神经网络辅助的GNSS/VO组合定位方法

针对当全球卫星导航系统(GNSS)信号失锁时,GNSS与视觉里程计(VO)组合定位方法定位精度下降的问题,提出一种基于长短期记忆(LSTM)神经网络辅助的GNSS/VO组合定位方法:在GNSS工作正常情况下,利用视觉里程计的位移增量和姿态构建LSTM的特征向量,将GNSS解算的位置增量作为输出对LSTM神经网络进行训练;GNSS信号失锁环境中,使用LSTM神经网络输出结果推算得到伪GNSS观测值,并将其与VO的结果进行松组合,实现GNSS/VO组合定位。实验结果表明,在GNSS信号丢失30、60、120s的过程中,所提方法的定位精度可分别提高约62%、64%、69%,证明该方法能够有效地提高GNSS/VO组合定位方法在GNSS拒止环境下的定位精度。

镍钛形状记忆合金骨植入物镍离子释放的体外测试方法

目的镍钛形状记忆合金骨植入物临床使用过程中存在镍离子析出的风险,植入物镍离子释放在体内很难直接测试,为此本文建立一种镍钛形状记忆合金骨植入物镍离子释放的体外测试方法。方法选用磷酸盐缓冲盐溶液(phosphate buffer salt solution,PBS)作为测试液,用于植入物镍离子的浸提,将植入物置于带盖容器中,容器中装有PBS,将容器静置在温度稳定控制的恒温培养箱内(37±1)℃下,静置相应设置的时间点,每个时间点设1组空白试验和3个相同平行试样进行镍释放量测定,分别用石墨炉原子吸收光谱仪(atomic absorption spectrometer,AAS)和电感耦合等离子体质谱仪(inductively coupled plasma mass spectrometry,ICP-MS)设备测试溶液中镍离子浓度。结果随时间推移从器械中释放出来的镍离子浓度整体呈下降趋势,镍离子释放时点为4周时,释放周期结束,AAS测得镍离子释放量平均值为0.37×10^(-3)μg/(cm^(2)·d),累计释放量平均值为10.22×10^(-3)μg/cm^(2)。ICP-MS测得镍离子释放量平均值为0.29×10^(-3)μg/(cm^(2)·d),累计释放量平均值为7.9×10^(-3)μg/cm^(2)。AAS和ICP-MS两种测试方法测试结果差异性不大,结果数据的走势相同说明试验可重复性高。结论此方法可为镍钛形状记忆合金骨植入物的风险评估提供依据,确保植入物在临床使用过程中镍离子的析出不会对人体造成伤害,保证其临床使用的安全性。

骨髓间充质干细胞机械刺激成骨的记忆效应

背景:骨髓间充质干细胞作为组织工程的理想种子细胞,已证实力学刺激有引导其分化的作用。研究表明,干细胞存在着机械记忆,即力学刺激所引起的改变可能会长期影响干细胞的命运。目的:观察骨髓间充质干细胞对力学刺激的记忆效应,解析其发生的表观遗传学机制。方法:(1)分离原代小鼠骨髓间充质干细胞,检测其三系分化能力,传至第3代待用;(2)利用拉伸仪,体外给予小鼠骨髓间充质干细胞机械刺激,检测细胞拉伸前后的成骨分化情况;(3)流式细胞仪检测拉伸后细胞干性;(4)将拉伸处理与未处理的细胞消化后铺种在常规12孔板,继续培养14 d后检测细胞的成骨分化情况;(5)免疫荧光染色检测并筛选造成干细胞机械记忆的关键组蛋白修饰变化,初步明确表观遗传调控的可能机制。结果与结论:(1)5%形变,0.5 Hz,6 h/d,为期7 d的机械刺激可以引导小鼠骨髓间充质干细胞向成骨方向分化;(2)重新铺种培养皿后,拉伸处理后的细胞仍能保持干性且继续向成骨方向分化;(3)结果显示,骨髓间充质干细胞对机械刺激存在记忆效应,这种效应可能与力学刺激导致的组蛋白H3K4me3修饰有关。

基于长短期记忆神经网络的自适应容错方法

针对传统的全球导航卫星系统/惯性导航系统(global navigation satellite system/inertial navigation system,GNSS/INS)紧组合系统容错方法对故障处理方式单一、环境适应性差的问题,提出了一种基于长短期记忆神经网络的自适应故障容错方法。该方法基于长短期记忆神经网络建立GNSS伪距、伪距率预测模型。发生故障时,通过分量检测法定位故障观测的维度,并引入相对差分定位精度分析故障观测对系统定位精度的影响,从而实现隔离与重构策略的动态选择。利用实测数据从可见星数、几何构型、故障持续时间3个角度设置多组环境进行仿真实验。仿真结果表明,所提方法对复杂环境具有更好的适应能力,可有效降低故障存续期间系统的定位误差,提高系统的故障检测性能。

High percentage of bone marrow CD8^(+)tissue-resident-like memory T cells predicts inferior survival in patients with acute myeloid leukemia

Tissue-resident memory T(TRM)cells infiltrating solid tumors could influence tumor progression and the response to immune therapies.However,the proportion and prognostic value of TRM cells in the bone marrow(BM)of patients with acute myeloid leukemia(AML)are unclear.In this study,we used flow cytometry to assay the phenotype of 49 BM samples from patients newly diagnosed with AML(ND-AML).We found that the BM CD8^(+)effector memory(TEM)cells highly expressed CD69(CD8^(+)TRM-like T cells),and their percentage was significantly increased in patients with ND-AML compared with that in healthy individuals(HI).The high percentage of CD8^(+)TRM-like subset was associated with poor overall survival in our ND-AML cohort.The Kaplan–Meier Plotter database verified a significantly reduced survival rate among patients with high expression of CD8^(+)TRM-like T cell characteristic genes(CD8A,CD69,and TOX),especially the M4 and M5 subtypes.Phenotypic analysis revealed that the BM CD8^(+)TRM-like subpopulation exhibited exhausted T cell characteristics,but its high expression of CD27 and CD28 and low expression of CD57 suggested its high proliferative potential.The single-cell proteogenomic dataset confirmed the existence of TRM-like CD8^(+)T cells in the BM of patients with AML and verified the high expression of immune checkpoints and costimulatory molecules.In conclusion,we found that the accumulation of BM CD8^(+)TRM-like cells could be an immune-related survival prediction marker for patients with AML.