Chemotaxonomy of Two Satureja L. （Lamiaceae） Species from Different Localities of Turkey

In this study, two Satureja L. species from different localities of Turkey （Satureja hortensis L. and Satureja boissieri Hausskn. ex Boiss.） have been studied to determine their taxonomic classification based on chemical characters. For this purpose, aerial parts of the studied taxa were investigated by HS-SPME/GC-MS. 28, 30 and 29 compounds were identified in S. boissieri from Sanhurfa, BingS1 and Malatya accounting from 90.80%, 91.31% and 92.67% of the whole oil, respectively. 26, 30 and 28 compounds were identified in S. hortensis from Adlyaman, Diyarbaklr, Elazl~ accounting from 93.20%, 92.85% and 92.42% of the whole oil, respectively, y-terpinene （30.4%, 26.5% and 32.1%）, carvacrol （26.4%, 25.2% and 23.3%） and p-cymene （10.5%, 13.2% and 10.4%） were determined as main compounds of S. boissieri in Sanhurfa, Bing6l and Malatya species, respectively. Carvacrol （25.0%, 34.1% and 32.1%）, thymol （28.2%, 20.2% and 28.1%） and y-terpinene （10.1%, 11.3% and 9.4%） were found as main constituents of S. hortensis in Adlyaman, Diyarbaklr and Elazl~ samples, respectively. The chemical distributions of the essential oil compounds in the Satureja pattern were discussed in respect to their chemotaxonomy and natural products.

Antioxidant properties and inhibitory effects of Satureja khozestanica essential oil on LDL oxidation induced-CuSO_4 in vitro

Objective:To assess various antioxidative activities of Satureja khozestanica essential oil(SKE)and its effect on oxidation of low density lipoprotein(LDL)induced by CuSO_4 in vitro by monitoring the formation of conjugated dienes and malondialdehyde(MDA).Methods:The formation of conjugated dienes,lag time and MDA were measured.Inhibition of this Cu-induced oxidation was studied in the presence of several concentrations of SKE.Also total antioxidant activity and free radical scavenging of SKE were determinated.Results:It was demonstrated that SKE was able to inhibit LDL oxidation and decrease the resistance of LDL against oxidation.The inhibitory effects of SKE on LDL oxidation were dose-dependent at concentrations ranging from 50 to 200μg/mL.Total antioxidant capacity of SKE was(3.20±0.40)nmol of ascorbic acid equivalents/g SKE.The SKE showed remarkable scavenging activity on 2,2-diphenyl-picrylhydrazyl,IC_(50)(5.30±0.11)ng/mL.Conclusions:This study shows that SKE is a source of potent antioxidants and prevents the oxidation of LDL in vitro and it may be suitable for use in food and pharmaceutical applications.

In vitro scolicidal effect of Satureja khuzistanica(Jamzad)essential oil

Objective:To investigate the scolicidal effect of the Satureja khuzistanica(S.khusistanica) essential oil from aerial parts of this herbal plant.Methods:The essential oil was obtained by hydrodistillation method.Gas chromatography(GC) and gas chromatography mass spectrometry(GC-MS) were employed to determine the chemical composition of the essential oil.Protoscolices were collected aseptically from sheep livers containing hydatid cyst.Protoscolices were exposed to various concentrations of the oil(3,5 and 10 mg/mL) for 10,20,30,and 60 min.Viability of protoscolices was confirmed by 0.1%eosin staining.Results:A total of 19 compounds representing 97.6%of the total oil,were identified.Carvacrol(94.9%) was found to be the major essential oil constituent.Scolicidal activity of S.khuzistanica essential oil at concentration of 3 mg/mL was 28.58,32.71,37.20 and 42.02%,respectively.This essential oil at concentration of 5 mg/ mL killed 51.33,66.68,81.12,and 100%.of protoscolices after 10,20,30 and 60 min,respectively. One hundred scolicidal effect was observed with S.khuzistanica essenlial oil at the concentration of 10 mg/mL after 10 min(comparing with 7.19%for control group).Conclusions:The essenlial oil of S.khuzistanica is rich in carvacrol and may be used as a natural scolicidal agent.

The Aromatic Plant <i>Satureja horvatii</i>ssp. <i>macrophylla</i>Induces Apoptosis and Cell Death to the A549 Cancer Cell Line

S. horvatii ssp. macrophylla (Lamiaceae) is an endemic aromatic plant of Greece and is used as a culinary herb. In the present study we explored its possible anticancer activity through the induction of apoptosis on cancer cells using A549 cell line as a model. The cytotoxic effects and the type of cell death (apoptosis/necrosis) were evaluated with biochemical and morphological criteria. The total methanol extract of S. horvatii ssp. macrophylla revealed forty two components, of which carvacrol was the major constituent (43.2%). Only a minor portion of them (3.41%) was contained in the active working plant extract solution which was used for cell treatment. This study indicates that extract of S. horvatii spp. macrophylla, which belongs to carvacrol chemotype, can induce both apoptosis and necrosis to A549 cancer cell line.

Effect of Different Levels of Chicory and Satureja Hortensis Root Powders on Performance and Carcass Characteristics of Broilers

Inulin and oligofructose that are used in animal nutrition originate from chicory roots. Chicory fructans were shown to be prebiotic （selectively interacting with intestinal bacterial ecosystem） in humans and in animals, including livestock and pets. This study was conducted to evaluate the effect of different levels of chicory and satureja hortensis root powders, which are commonly used as alternative to antibiotics, on performance and carcass characteristic of broilers. The study included 280 Ross 308 male broiler chicks （1-day old at the beginning of the study）. There were 7 treatment groups, each consisting of 4 replicates （10 chicks in each replicate）. Chicory root powder （CRP） were added in 2%, 4% and 6% doses and satureja hortensis root powder （SHRP） were added in 0.2%, 0.4% and 0.6% doses to starter and grower diets. The experiment lasted 42 days. Results showed that adding 2% of chicory root powder to the diet rather than control group, had significant effect on body weight gain （BWG）, feed intake （FI） and feed conversion rate （FCR）. All treatments of SHRP rather than control group significantly decreased FCR and feed intake. Treating with 6% CRP in days 1 to 21 in contrast to control group significantly decreased feed intake, but hadn＇t significant effect on FCR. Consequently we concluded that, using 2% CRP and different levels of SHRP had significant effect on performance and carcass characteristics of broilers.

Glycosidically Bound Volatile Compounds of Satureja montana L.,S.cuneifolia Ten.,S.subspicata Vis.and Endemic S.visianii SiliC

In this paper, the glycoconjugated volatile compounds of four Croatian Satureja species (Satureja montana L., S. cuneifolia Ten., S. subspicata Vis. and endemic S. visianii Silic) were investigated. Content and composition of these compounds were examined depending on the stage of plant development. GC and GC–MS analysis of volatile aglycones revealed twenty-one compounds. Thymoquinone, geraniol and carvacrol were detected in all vegetative phases of the investigated plants. Other quantitatively important aglycones were eugenol and thymol of S. montana, phenyl ethyl alcohol, benzene acetaldehyde, borneol, α-terpineol, thymol and eugenol of S. cuneifolia, phenyl ethyl alcohol, benzene acet-aldehyde, terpinen-4-ol, α-terpineol and β-ionone of S. subspicata and camphor, thymol and 8a-acetoxylemolol of S. visianii. Moderate similarity in the chemical composition of essential oils and volatile aglycones of investigated plant species indicate that many biologically active compounds are glycosylated and accumulate as non-volatile glycosides.

Amelioration of altered antioxidant enzyme activity by Satureja khuzistanica essential oil in alloxan-induced diabetic rats

AIM: To examine the possible protective effect of Satureja khuzistanica essential oil(SKE) on antioxidant enzyme activity in alloxan-induced Type 1 diabetic rats. METHOD: Thirty Sprague-Dawley male rats were divided into three groups randomly; group one as control, group two diabetic, with no treatment, and group three treatment with SKE at 500 ppm in drinking water, respectively. Diabetes was induced in the second and third groups by alloxan injection subcutaneously. After eight weeks, animals were anaesthetized. Blood samples were also collected before killing to measure antioxidant enzymes activity. RESULTS: SKE significantly increased the serum level of glutathione and the serum activity of glutathione peroxidase, superoxide dismutase, and catalase in the treated group compared with the diabetic untreated group. CONCLUSION: The findings showed that SKE exerts beneficial effects on the antioxidant enzymes activity in alloxan-induced Type 1 diabetic rats.