[Objective] The study aimed to explore the antibacterial activity and stability of antagonistic bacteria P72 against wheat scab.[Method] The Bacillus subtilis P72 was inoculated into fermentation media with the inocul...[Objective] The study aimed to explore the antibacterial activity and stability of antagonistic bacteria P72 against wheat scab.[Method] The Bacillus subtilis P72 was inoculated into fermentation media with the inoculation amount 5%,then cultured on shaking table at 28 ℃ for 48 h and centrifuged at 8 000 r/min for 10 min.The supernatant of fermentation broth was purified and then the genetic stability,thermal stability and pH stability were detected.[Result] By DEAE-52 ion exchange chromatography,the protein eluted by 0.5 mol/L NaCl solution possessed the strongest antagonistic ability against wheat scab pathogen.The SDS-PAGE electrophoresis showed that the molecular weight of the purified protein with antibacterial effect was 40 kD.By stability test,the antibacterial substance produced by P72 strain showed heritable antagonistic activity,high stability below 60 ℃,stability to acid and unstability to alkali.[Conclusion] The antagonistic bacteria P72 had strong antagonistic ability to wheat scab pathogen,stable antibacterial activity and thermal stability,so it would possess a wide development prospect.展开更多
A brief review on the development of wheat germplasm with introduced powdery mildew and scab resistance from Haynaldia villosa Sch. and Leymus racemosus Lam., Roegneria ciliaris (Trin.) Nevski as well as R. kamoji C. ...A brief review on the development of wheat germplasm with introduced powdery mildew and scab resistance from Haynaldia villosa Sch. and Leymus racemosus Lam., Roegneria ciliaris (Trin.) Nevski as well as R. kamoji C. Koch respectively was made. In the course of germplasm development, genome analysis by means of chromosome banding, genomic in situ hybridization (GISH) or fluorescence in situ hybridization (FISH), molecular markers, particularly restriction fragment length polymorphism (RFLP) coupled with aneuploid analysis was employed for the purpose of improving breeding efficiency. Potential use of such germplasm in wheat breeding practice, basic studies and some related problems were also discussed.展开更多
The paper was to explore the effect of trace and secondary elements on scab of cucurbits ( Cladosporium cucumerinum) and their effects on enzyme activities of cucumber plants. [ Method ] Indoor antifungd experiment ...The paper was to explore the effect of trace and secondary elements on scab of cucurbits ( Cladosporium cucumerinum) and their effects on enzyme activities of cucumber plants. [ Method ] Indoor antifungd experiment and pot test was carried out to study the control effect of different trace and secondary dements on scab of cucu.rbits; after trace and secondary element compounds were sprayed for 7 d, the content of soluble protein and malondialdehyde (MDA) and the activities of peroxidase (POD) and superoxide dismutase (SOD) of cucumber leaves during seedling stage were simultaneously detected. [ Result] The inhibi- tion effects on scab of cucurbits were found in the three trace and secondary dements, especially for the CaC12 (66.9%), and about 54.6% and 24.1% for ZnSO4 and H3 BO3 , respectively. When cucumber plants were infected by scab of cuea.rbits, the contents of MDA for all treatments were significantly decreased in cucum- ber leaves after spraying the three trace and secondary elements compared with control. The contents of soluble protein in cucumber seedling were increased by Zn- SO4 and H3 BO3 application, while the contents of POD and SOD were increased on ZnSO4 and CaCI2 treatments. The defense system of cucumber was induced and strengthened by application of the three trace and secondary elements. [ Conclusion ] The results provided the theoretical basis for practical application and research on resistance mechanism of using trace and secondary dements to control scab of eueurbits.展开更多
The finite-depth concrete panels have been widely applied in the protective structures,and its impact resistance and dynamic fracture failures,especially the scabbing/perforation limits,under high velocity projectile ...The finite-depth concrete panels have been widely applied in the protective structures,and its impact resistance and dynamic fracture failures,especially the scabbing/perforation limits,under high velocity projectile impact,are mainly concerned by protective engineers,which are numerically studied based on an improved dynamic concrete model in this study.Firstly,based on the framework of the KCC(Karagozian&Case concrete)model,a dynamic concrete model is proposed which considers an independent tensile damage model and a continued transition between dynamic tensile and compressive properties.Secondly,the strength surface,equation of state and damage parameters of the proposed model are comprehensively calibrated by a triaxial compressive test with high confinement pressure,the rationality of which is further verified based on the single element tests,e.g.,uniaxial and triaxial compression as well as uniaxial,biaxial and triaxial tension.Thirdly,a series of projectile high velocity impact tests on thin and thick concrete panels are simulated,which indicates that the projectile residual velocity and dynamic fracture failures are reproduced satisfactorily,while the KCC model underestimates both the spalling and scabbing dimensions severely.Finally,based on the validated concrete model and finite element analyses approach,the validations of the existing five empirical formulae are evaluated,in terms of the depth of penetration(DOP)and scabbing/perforation limits of concrete panel.Both the Army corps of engineers(ACE)and modified National Defense Research Committee(NDRC)formulae are recommended in the design of the protective structure to avoid scabbing failure.展开更多
Fusarium head blight(FHB), caused by Fusarium graminearum, is one of the most destructive wheat(Triticum aestivum L.) diseases worldwide. Identification of quantitative trait loci(QTL) conferring FHB resistance follow...Fusarium head blight(FHB), caused by Fusarium graminearum, is one of the most destructive wheat(Triticum aestivum L.) diseases worldwide. Identification of quantitative trait loci(QTL) conferring FHB resistance followed by marker assisted selection(MAS) is an efficient approach to breed FHB-resistant varieties. In this study, 38 additive QTL and 18 pairs of epistatic QTL for FHB resistance were detected in four environments using a population of recombinant inbred lines(RILs) derived from varieties Neixiang 188 and Yanzhan 1. Six QTL clusters were located on chromosomes 2D, 4B, 4D, 5A, 5D and 7B, suggesting possible polytrophic functions. Six elite lines with good FHB resistance and agronomic traits were selected from the same population using the associated markers. Our results suggest that MAS of multiple QTL will be effective and efficient in wheat breeding.展开更多
In Agriculture Sciences, detection of diseases is one of the mostchallenging tasks. The mis-interpretations of plant diseases often lead towrong pesticide selection, resulting in damage of crops. Hence, the automaticr...In Agriculture Sciences, detection of diseases is one of the mostchallenging tasks. The mis-interpretations of plant diseases often lead towrong pesticide selection, resulting in damage of crops. Hence, the automaticrecognition of the diseases at earlier stages is important as well as economicalfor better quality and quantity of fruits. Computer aided detection (CAD)has proven as a supportive tool for disease detection and classification, thusallowing the identification of diseases and reducing the rate of degradationof fruit quality. In this research work, a model based on convolutional neuralnetwork with 19 convolutional layers has been proposed for effective andaccurate classification of Marsonina Coronaria and Apple Scab diseases fromapple leaves. For this, a database of 50,000 images has been acquired bycollecting images of leaves from apple farms of Himachal Pradesh (H.P)and Uttarakhand (India). An augmentation technique has been performedon the dataset to increase the number of images for increasing the accuracy.The performance analysis of the proposed model has been compared with thenew two Convolutional Neural Network (CNN) models having 8 and 9 layersrespectively. The proposed model has also been compared with the standardmachine learning classifiers like support vector machine, k-Nearest Neighbour, Random Forest and Logistic Regression models. From experimentalresults, it has been observed that the proposed model has outperformed theother CNN based models and machine learning models with an accuracy of99.2%.展开更多
58 winter wheat breeding lines and 4 widely popularized varieties in Huaihe River wheat production regions of Anhui were used to screen resistant germplasms to Fusarium head blight. Incidence, disease index, percentag...58 winter wheat breeding lines and 4 widely popularized varieties in Huaihe River wheat production regions of Anhui were used to screen resistant germplasms to Fusarium head blight. Incidence, disease index, percentage of infected kernels and DON content in the kernels were measured for each variety or line following artificial inoculation of Fusarium spp. pathogens. Reactions of wheat to Fusarium infection varied among genotypes and different types of resistance. The incidence of infection for each variety or line highly correlated to the severity of colonization after infection. DON content was more correlated to the percentage of kernels infected than to the overall incidence or severity. The selected control varieties were all susceptible to FHB, while seven breeding lines demonstrated good resistant performance. New FHB resistant winter wheat varieties appealing to the actual needs are expected from them.展开更多
[Objective] The paper was to select different fungicides for field efficacy test against pear scab in summer.[Method] Five treatments were designed in the test,including 10% difenoconazole WDG 6 000 times dilution,50%...[Objective] The paper was to select different fungicides for field efficacy test against pear scab in summer.[Method] Five treatments were designed in the test,including 10% difenoconazole WDG 6 000 times dilution,50% captan WP 600 times dilution,30% pyraclostrobin SC 2 000 times dilution,430 g/L tebuconazole 4 000 times dilution,and water(control).[Result] 10% Difenoconazole WDG 6 000 times dilution,50% captan WP 600 times dilution,30% pyraclostrobin SC 2 000 times dilution,430 g/L tebuconazole SC 4 000 times dilution had good control effects against pear scab,and the efficacies were 89.13%-93.51%.[Conclusion] The results will provide the theoretical basis for field control of pear scab in summer.展开更多
In order to identify the resistance of peanut varieties against scab disease, the changes of physiological characteristics and yield in two peanut varieties 'Heyou 13' and 'Quanhual0' with different resistance eff...In order to identify the resistance of peanut varieties against scab disease, the changes of physiological characteristics and yield in two peanut varieties 'Heyou 13' and 'Quanhual0' with different resistance effected by peanut scab were stud- ied in field experiment by spray. The results showed the soluble sugar increased firstly and then decreased both in the resistant and susceptible peanuts after inocu- lation. Compared with the control, the soluble sugar of resistant peanut was higher, and that of the susceptible peanut was lower, which implied that the peanut resis- tance to scab was positive correlated with soluble sugar content. The soluble pro- tein content in leaves of resistant and susceptible peanut varieties after inoculation was higher than that of the control. The peak of soluble protein content appeared earlier in the susceptible peanut than in the resistant peanut, which indicated that the susceptible peanut were more sensitive to the pathogen than the resistant peanut. The leaf SOD activity increased firstly and then decreased both in resistant and susceptible varieties, while the decrease in the resistant variety were lower than in the susceptible variety. With the growth process, the CAT and POD activities first decreased, then increased, and again decreased. Compared with the corresponding control, the resistant variety had relatively higher CAT and POD activities than the susceptible variety, which illustrated that the protective enzymes played important roles in resistance mechanism to scab. The MDA content of the two peanut vari- eties after inoculation increased, indicating that plasma membranes were subjected to different degrees of damage. The yield of resistant and susceptible varieties after inoculation reduced, while the yield of the susceptible peanut of 'Quanhua 10' reduced greater.展开更多
With the increasing prevalence frequency and aggravating harm degree in the 21st century, wheat scab had become a recurrent disease in eastern wheat area of Jiangsu Province. This paper reviewed general occurrence si...With the increasing prevalence frequency and aggravating harm degree in the 21st century, wheat scab had become a recurrent disease in eastern wheat area of Jiangsu Province. This paper reviewed general occurrence situation of wheat scab in eastern Jiangsu, and summarized its occurrence characteristics in last decade. The disease prevalence was elaborated from the aspects of fungal source quantity, chmatic conditions, cultivation modes, variety resistance and pesticide resistance. The author put forward a series of measures and countermeasures, including strengthening resistance monitoring and early warning, promoting and cultivating disease-resistant varieties, compressing planting area of direct seeding rice, canting out chemical control scientifically, and realizing unified prevention and control, in order to provide a scientific basis for the control of wheat scab.展开更多
[Objective] This paper was to improve the current status that the fungicide carbendazim as the single agent was largely applied in the control against wheat scab. [ Method] Five kinds of fungicides on sales and new ag...[Objective] This paper was to improve the current status that the fungicide carbendazim as the single agent was largely applied in the control against wheat scab. [ Method] Five kinds of fungicides on sales and new agents against wheat scab were selected for field trials. [ Result] 25% JS399 - 19 SC, 30% te- buconazoh · thiram WP, 75% trifloxystrobin · tebuconasole WDG and 10% ZJ0712 JG could effectively control wheat scab, and the control effects of one time or two times application were better than control agent carbendasim; the control efficacy of two times application was improved in different degrees against one time ap- vlication. [ Conclusion] The naner vrevided basis for the control atminst wheat scab.展开更多
The cucumber scab caused by Cladosporium cucumerinum Ell. is one of the most important diseases of cucumber in the PR. of China and elsewhere. Recently, wider acceptance of under glas culture exacerbates the problem. ...The cucumber scab caused by Cladosporium cucumerinum Ell. is one of the most important diseases of cucumber in the PR. of China and elsewhere. Recently, wider acceptance of under glas culture exacerbates the problem. Both in greenhouses and in free fields in the Northern China pose a challenge in the control of this disease, because there are no effective fungicide to be used. Induced resistance with chemicals may be an ideal way to protect cucumber against this disease , if there are no resistant cultivars to be used. This paper reports the effect of BION, a new activator of SAR, on the induction of resistance to cucumber scab using two treatment methods (seed treatment and foliar spray) at different concentrations (10,20,40, 80, 160 mg. mL- 1). The results showed that the number of diseased plants was decreased drastically in BION - treated plants, the disease incidence was 35 %, whereas it was 75 % in control plants. The disease severity of plant with BION treatment by foliar spray was 2 and it was 1.5 if used as seed treatment, whereas it was 3 and 3.1 in control plants respectively. Moreover it has no phytotoxic effect to be observed at concentrations used in the experiment.展开更多
Fusarium head blight(FHB),also known as scab,is a devastating fungal disease of wheat that causes significant losses in grain yield and quality.Quantitative inheritance and cumbersome phenotyping make FHB resistance a...Fusarium head blight(FHB),also known as scab,is a devastating fungal disease of wheat that causes significant losses in grain yield and quality.Quantitative inheritance and cumbersome phenotyping make FHB resistance a challenging trait for direct selection in wheat breeding.Genomic selection to predict FHB resistance traits has shown promise in several studies.Here,we used univariate and multivariate genomic prediction models to evaluate the prediction accuracy(PA)for different FHB traits using 476elite and advanced breeding lines developed by South Dakota State University hard winter wheat breeding program.These breeding lines were assessed for FHB disease index(DIS),and percentage of Fusarium damaged kernels(FDK)in three FHB nurseries in 2018,2019,and 2020(TP18,TP19,and TP20)and were evaluated as training populations(TP)for genomic prediction(GP)of FHB traits.We observed a moderate PA using univariate models for DIS(0.39 and 0.35)and FDK(0.35 and 0.37)using TP19 and TP20,respectively,while slightly higher PA was observed(0.41 for DIS and 0.38 for FDK)when TP19 and TP20(TP19+20)were combined to leverage the advantage of a large training population.Although GP with multivariate approach including plant height and days to heading as covariates did not significantly improve PA for DIS and FDK over univariate models,PA for DON increased by 20%using DIS,FDK,DTH as covariates using multi-trait model in 2020.Finally,we used TP19,TP20,and TP19+20 in forward prediction to calculate genomic-estimated breeding values(GEBVs)for DIS and FDK in preliminary breeding lines at an early stage of the breeding program.We observed moderate PA of up to 0.59 for DIS and 0.54for FDK,demonstrating the promise in genomic prediction for FHB resistance in earlier stages using advanced lines.Our results suggest GP for expensive FHB traits like DON and FDK can facilitate the rejection of highly susceptible materials at an early stage in a breeding program.展开更多
Based on the survey of observation, the factors afffecting prevalence degree of wheat scab ( Gibberella zeae) were analyzed through coupling method. The results showed that the average diseased ear rate before harve...Based on the survey of observation, the factors afffecting prevalence degree of wheat scab ( Gibberella zeae) were analyzed through coupling method. The results showed that the average diseased ear rate before harvest (disease index) was associated with the average relative humidity from April to early May, the average temperature from April to early May, and the interval from peak maturity stage of perithecium to full heading stage of wheat. The interval was used to measure the coincidence degree between release period of ascospore and ear period ( heading to grouting stage). The prevalence of wheat scab was determined by coincidence degree and meteorological conditions of ear period. Quantitative prediction should be conducted, to improve prediction and control levels.展开更多
The research was focused on the persistence of resistance of V. inaequalis to strobilurins from resistant populations occurring in the field. It was studied with two types of experiments: the first in apple orchards w...The research was focused on the persistence of resistance of V. inaequalis to strobilurins from resistant populations occurring in the field. It was studied with two types of experiments: the first in apple orchards where resistance occurred in 2005 and employ of these fungicides was suspended from 2006 to 2011, realizing every year sensitivity/resistance assays on strobilurins. In the second was verified the sensitivity/resistance of the same 2015 populations, repeatedly multiplied (ten inoculations) in glasshouse on apple seedlings. Results showed that the resistance survived in orchards all the years of tests (six years), despite sensitivity fluctuations occurred on infected leaves due to sexual crosses, competition with sensible strains and soil management. In glasshouse, the level of resistance of all populations increased quickly in first inoculations and values remained high (EC50 > 10 mg·L-1) in all the following inoculations, until the last, the 10th. V. inaequalis resistant strains seemed to show high fitness: relevant persistence in the field, and a capacity of prevail on sensible ones in absence of factors of field variability.展开更多
Dithianon is a multi-site fungicide and has never been object of suspects and reports of reduced sensitivity and activity. Italian IFP technicians had the suspect of reductions of activity by this fungicide on </sp...Dithianon is a multi-site fungicide and has never been object of suspects and reports of reduced sensitivity and activity. Italian IFP technicians had the suspect of reductions of activity by this fungicide on </span><i><span style="font-family:Verdana;">Venturia inaequalis.</span></i><span style="font-family:Verdana;"> Methodologies, </span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> and </span><i><span style="font-family:Verdana;">in vivo</span></i><span style="font-family:Verdana;"> were carried out to verify this suspect. Populations poorly controlled with suspects on dithianon and sensible ones </span><span><span style="font-family:Verdana;">were utilized. The tests </span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> permitted to evidence light and non-significant</span></span><span style="font-family:Verdana;"> reductions of sensitivity of poorly controlled populations </span></span><span style="font-family:Verdana;">with </span><span style="font-family:Verdana;">respect </span><span style="font-family:Verdana;">to </span><span style="font-family:""><span style="font-family:Verdana;">sensible ones. </span><i><span style="font-family:Verdana;">In vivo tests</span></i><span style="font-family:Verdana;"> on seedlings were non</span></span><span style="font-family:Verdana;">-</span><span style="font-family:""><span style="font-family:Verdana;">reliable for a general low activity of dithianon. On the contrary, the original </span><i><span style="font-family:Verdana;">in vivo</span></i><span style="font-family:Verdana;"> methodology on grafted apple plants showed several reductions of activity, with moderate levels and a spot distribution in orchards. The cause was probably due to the increase</span></span><span style="font-family:Verdana;">d</span><span style="font-family:Verdana;"> treatments with dithianon caused by problems on other groups of fungicides and by a high infective pressure in some years. It is discussed if this reduction can be considered a resistance phenomenon or a temporary modification of the interactions </span><span style="font-family:Verdana;">of </span><span style="font-family:Verdana;">plant-fungus-fungicide.展开更多
Among the most economically important fruit crops in South and North Kyrgyzstan affected by scab disease are apples (Malus domestica). Natural isolates of Venturia inaequalis were isolated from diseased parts of apple...Among the most economically important fruit crops in South and North Kyrgyzstan affected by scab disease are apples (Malus domestica). Natural isolates of Venturia inaequalis were isolated from diseased parts of apple trees, selected from different regions, through sedimentation of air microflora on the crowns of fruit trees. Two biocontrol agents as Trichoderma viride and Streptomyces sр. were tested for apple scab control in vitro and field conditions. Two applications of Trichoderma viride within 35 days completely stopped the scab disease in seedling leaves. Within 40 days, the seedlings were recovered;the new leaves have blossomed on branches. The Streptomyces sp. antagonists were inferior in effectiveness compared to Trichoderma viride;nevertheless, they prevented progression of the disease in the leaves. Seedlings grew new shoots and leaves around 50 days after the second treatment. Orchard experiment results provide great hope that a biological product based on Trichoderma viride could work as an effective agent to suppress the development of the pathogen in the early spring, when leaves start to show scab disease symptoms.展开更多
Biological methodologies (</span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> and </span><i><span ...Biological methodologies (</span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> and </span><i><span style="font-family:Verdana;">in vivo</span></i><span style="font-family:Verdana;">) were tested on </span><i><span style="font-family:Verdana;">Venturia inaequalis</span></i><span style="font-family:Verdana;"> to assay sensitivity of populations treated with the SDHI penthiopyrad, fluxapyroxad and fluopyram that showed a field poor control in an experimental Centre. In comparison, sensible populations were tested. </span><i><span style="font-family:Verdana;">I</span></i><i><span style="font-family:Verdana;">n vitro</span></i><span style="font-family:Verdana;"> trials, the poorly controlled populations presented moderate increases of EC</span><sub><span style="font-family:Verdana;">50</span></sub><span style="font-family:Verdana;"> and RF values in all three years in which tests were carried out. The </span><i><span style="font-family:Verdana;">in vivo</span></i><span style="font-family:Verdana;"> trials in glasshouse showed that the activity of SDHI fungicides on field poorly controlled populations was highly reduced. These populations can be defined as “resistant” as the high loss of sensitivity and activity were repeatedly assessed in field and glasshouse. Trials showed that these reductions were manifested by curative applications and not by preventive ones, as already ascertained for </span><i><span style="font-family:Verdana;">V. inaequalis </span></i><span style="font-family:Verdana;">populations resistant to AP and DMI fungicides. </span><span><span style="font-family:Verdana;">The cross-resistance of resistant populations to the three SDHIs was highly positive among them. In bibliography, there are only researches of cross-resistance among SDHIs starting from isolates resistant to boscalid, with results of different sensitivities and cross-resistance. These results with populations of </span><i><span style="font-family:Verdana;">V. inaequalis</span></i><span style="font-family:Verdana;"> resistant to the three SDHI offer practical evaluations in cross-resistance.展开更多
Little is written on the management of an innocuouslooking skin scab over an autogenous arteriovenous fistula(AVF)used for haemodialysis.The seriousness of the underlying pathology can be under-estimated,and this may ...Little is written on the management of an innocuouslooking skin scab over an autogenous arteriovenous fistula(AVF)used for haemodialysis.The seriousness of the underlying pathology can be under-estimated,and this may lead to early loss of the AVF,and majorlife-threatening haemorrhage.We describe the management of a 78-year-old patient presenting with an innocuous-looking scab over an AVF and review the pertinent literature on this subject.展开更多
文摘[Objective] The study aimed to explore the antibacterial activity and stability of antagonistic bacteria P72 against wheat scab.[Method] The Bacillus subtilis P72 was inoculated into fermentation media with the inoculation amount 5%,then cultured on shaking table at 28 ℃ for 48 h and centrifuged at 8 000 r/min for 10 min.The supernatant of fermentation broth was purified and then the genetic stability,thermal stability and pH stability were detected.[Result] By DEAE-52 ion exchange chromatography,the protein eluted by 0.5 mol/L NaCl solution possessed the strongest antagonistic ability against wheat scab pathogen.The SDS-PAGE electrophoresis showed that the molecular weight of the purified protein with antibacterial effect was 40 kD.By stability test,the antibacterial substance produced by P72 strain showed heritable antagonistic activity,high stability below 60 ℃,stability to acid and unstability to alkali.[Conclusion] The antagonistic bacteria P72 had strong antagonistic ability to wheat scab pathogen,stable antibacterial activity and thermal stability,so it would possess a wide development prospect.
文摘A brief review on the development of wheat germplasm with introduced powdery mildew and scab resistance from Haynaldia villosa Sch. and Leymus racemosus Lam., Roegneria ciliaris (Trin.) Nevski as well as R. kamoji C. Koch respectively was made. In the course of germplasm development, genome analysis by means of chromosome banding, genomic in situ hybridization (GISH) or fluorescence in situ hybridization (FISH), molecular markers, particularly restriction fragment length polymorphism (RFLP) coupled with aneuploid analysis was employed for the purpose of improving breeding efficiency. Potential use of such germplasm in wheat breeding practice, basic studies and some related problems were also discussed.
基金Supported by Liaoning Natural Science Foundation Project(20062124)~~
文摘The paper was to explore the effect of trace and secondary elements on scab of cucurbits ( Cladosporium cucumerinum) and their effects on enzyme activities of cucumber plants. [ Method ] Indoor antifungd experiment and pot test was carried out to study the control effect of different trace and secondary dements on scab of cucu.rbits; after trace and secondary element compounds were sprayed for 7 d, the content of soluble protein and malondialdehyde (MDA) and the activities of peroxidase (POD) and superoxide dismutase (SOD) of cucumber leaves during seedling stage were simultaneously detected. [ Result] The inhibi- tion effects on scab of cucurbits were found in the three trace and secondary dements, especially for the CaC12 (66.9%), and about 54.6% and 24.1% for ZnSO4 and H3 BO3 , respectively. When cucumber plants were infected by scab of cuea.rbits, the contents of MDA for all treatments were significantly decreased in cucum- ber leaves after spraying the three trace and secondary elements compared with control. The contents of soluble protein in cucumber seedling were increased by Zn- SO4 and H3 BO3 application, while the contents of POD and SOD were increased on ZnSO4 and CaCI2 treatments. The defense system of cucumber was induced and strengthened by application of the three trace and secondary elements. [ Conclusion ] The results provided the theoretical basis for practical application and research on resistance mechanism of using trace and secondary dements to control scab of eueurbits.
基金supported by the National Natural Science Foundation of China(Grant No.52208500)。
文摘The finite-depth concrete panels have been widely applied in the protective structures,and its impact resistance and dynamic fracture failures,especially the scabbing/perforation limits,under high velocity projectile impact,are mainly concerned by protective engineers,which are numerically studied based on an improved dynamic concrete model in this study.Firstly,based on the framework of the KCC(Karagozian&Case concrete)model,a dynamic concrete model is proposed which considers an independent tensile damage model and a continued transition between dynamic tensile and compressive properties.Secondly,the strength surface,equation of state and damage parameters of the proposed model are comprehensively calibrated by a triaxial compressive test with high confinement pressure,the rationality of which is further verified based on the single element tests,e.g.,uniaxial and triaxial compression as well as uniaxial,biaxial and triaxial tension.Thirdly,a series of projectile high velocity impact tests on thin and thick concrete panels are simulated,which indicates that the projectile residual velocity and dynamic fracture failures are reproduced satisfactorily,while the KCC model underestimates both the spalling and scabbing dimensions severely.Finally,based on the validated concrete model and finite element analyses approach,the validations of the existing five empirical formulae are evaluated,in terms of the depth of penetration(DOP)and scabbing/perforation limits of concrete panel.Both the Army corps of engineers(ACE)and modified National Defense Research Committee(NDRC)formulae are recommended in the design of the protective structure to avoid scabbing failure.
基金supported by the National R&D Project of Transgenic Crops of the Ministry of Science and Technology of Chinathe Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘Fusarium head blight(FHB), caused by Fusarium graminearum, is one of the most destructive wheat(Triticum aestivum L.) diseases worldwide. Identification of quantitative trait loci(QTL) conferring FHB resistance followed by marker assisted selection(MAS) is an efficient approach to breed FHB-resistant varieties. In this study, 38 additive QTL and 18 pairs of epistatic QTL for FHB resistance were detected in four environments using a population of recombinant inbred lines(RILs) derived from varieties Neixiang 188 and Yanzhan 1. Six QTL clusters were located on chromosomes 2D, 4B, 4D, 5A, 5D and 7B, suggesting possible polytrophic functions. Six elite lines with good FHB resistance and agronomic traits were selected from the same population using the associated markers. Our results suggest that MAS of multiple QTL will be effective and efficient in wheat breeding.
基金This work was supported by Taif University Researchers Supporting Project(TURSP)under number(TURSP-2020/73),Taif University,Taif,Saudi Arabia.
文摘In Agriculture Sciences, detection of diseases is one of the mostchallenging tasks. The mis-interpretations of plant diseases often lead towrong pesticide selection, resulting in damage of crops. Hence, the automaticrecognition of the diseases at earlier stages is important as well as economicalfor better quality and quantity of fruits. Computer aided detection (CAD)has proven as a supportive tool for disease detection and classification, thusallowing the identification of diseases and reducing the rate of degradationof fruit quality. In this research work, a model based on convolutional neuralnetwork with 19 convolutional layers has been proposed for effective andaccurate classification of Marsonina Coronaria and Apple Scab diseases fromapple leaves. For this, a database of 50,000 images has been acquired bycollecting images of leaves from apple farms of Himachal Pradesh (H.P)and Uttarakhand (India). An augmentation technique has been performedon the dataset to increase the number of images for increasing the accuracy.The performance analysis of the proposed model has been compared with thenew two Convolutional Neural Network (CNN) models having 8 and 9 layersrespectively. The proposed model has also been compared with the standardmachine learning classifiers like support vector machine, k-Nearest Neighbour, Random Forest and Logistic Regression models. From experimentalresults, it has been observed that the proposed model has outperformed theother CNN based models and machine learning models with an accuracy of99.2%.
基金Supported by the Sci-Tech Innovative Project of Anhui Academy of Agricultural Sciences(13A0207)the Special Fund for Agro-scientific Research in the Public Interest(201503112-12)
文摘58 winter wheat breeding lines and 4 widely popularized varieties in Huaihe River wheat production regions of Anhui were used to screen resistant germplasms to Fusarium head blight. Incidence, disease index, percentage of infected kernels and DON content in the kernels were measured for each variety or line following artificial inoculation of Fusarium spp. pathogens. Reactions of wheat to Fusarium infection varied among genotypes and different types of resistance. The incidence of infection for each variety or line highly correlated to the severity of colonization after infection. DON content was more correlated to the percentage of kernels infected than to the overall incidence or severity. The selected control varieties were all susceptible to FHB, while seven breeding lines demonstrated good resistant performance. New FHB resistant winter wheat varieties appealing to the actual needs are expected from them.
基金Supported by Youth Fund Project of Hebei Academy of Agriculture and Forestry Sciences(2018020101)Science and Technology Plan of Hebei Province (16246327D)Special Financial Project of Hebei Province (F18R1908,2019-3-5-2)。
文摘[Objective] The paper was to select different fungicides for field efficacy test against pear scab in summer.[Method] Five treatments were designed in the test,including 10% difenoconazole WDG 6 000 times dilution,50% captan WP 600 times dilution,30% pyraclostrobin SC 2 000 times dilution,430 g/L tebuconazole 4 000 times dilution,and water(control).[Result] 10% Difenoconazole WDG 6 000 times dilution,50% captan WP 600 times dilution,30% pyraclostrobin SC 2 000 times dilution,430 g/L tebuconazole SC 4 000 times dilution had good control effects against pear scab,and the efficacies were 89.13%-93.51%.[Conclusion] The results will provide the theoretical basis for field control of pear scab in summer.
基金Supported by the Earmarked Fund for Modern Agroindustry Technology Research System of China(CARS-13)Key Project of Science and Technology Plan of Quanzhou(2012N76)~~
文摘In order to identify the resistance of peanut varieties against scab disease, the changes of physiological characteristics and yield in two peanut varieties 'Heyou 13' and 'Quanhual0' with different resistance effected by peanut scab were stud- ied in field experiment by spray. The results showed the soluble sugar increased firstly and then decreased both in the resistant and susceptible peanuts after inocu- lation. Compared with the control, the soluble sugar of resistant peanut was higher, and that of the susceptible peanut was lower, which implied that the peanut resis- tance to scab was positive correlated with soluble sugar content. The soluble pro- tein content in leaves of resistant and susceptible peanut varieties after inoculation was higher than that of the control. The peak of soluble protein content appeared earlier in the susceptible peanut than in the resistant peanut, which indicated that the susceptible peanut were more sensitive to the pathogen than the resistant peanut. The leaf SOD activity increased firstly and then decreased both in resistant and susceptible varieties, while the decrease in the resistant variety were lower than in the susceptible variety. With the growth process, the CAT and POD activities first decreased, then increased, and again decreased. Compared with the corresponding control, the resistant variety had relatively higher CAT and POD activities than the susceptible variety, which illustrated that the protective enzymes played important roles in resistance mechanism to scab. The MDA content of the two peanut vari- eties after inoculation increased, indicating that plasma membranes were subjected to different degrees of damage. The yield of resistant and susceptible varieties after inoculation reduced, while the yield of the susceptible peanut of 'Quanhua 10' reduced greater.
基金Supported by Independent Innovation Project of Agricultural Science and Technology in Jiangsu Province[CX(14)2126]
文摘With the increasing prevalence frequency and aggravating harm degree in the 21st century, wheat scab had become a recurrent disease in eastern wheat area of Jiangsu Province. This paper reviewed general occurrence situation of wheat scab in eastern Jiangsu, and summarized its occurrence characteristics in last decade. The disease prevalence was elaborated from the aspects of fungal source quantity, chmatic conditions, cultivation modes, variety resistance and pesticide resistance. The author put forward a series of measures and countermeasures, including strengthening resistance monitoring and early warning, promoting and cultivating disease-resistant varieties, compressing planting area of direct seeding rice, canting out chemical control scientifically, and realizing unified prevention and control, in order to provide a scientific basis for the control of wheat scab.
基金Supported by Science and Technology Support Project for Functional Areas of Food Production in Ningbo City (200903C1011009)
文摘[Objective] This paper was to improve the current status that the fungicide carbendazim as the single agent was largely applied in the control against wheat scab. [ Method] Five kinds of fungicides on sales and new agents against wheat scab were selected for field trials. [ Result] 25% JS399 - 19 SC, 30% te- buconazoh · thiram WP, 75% trifloxystrobin · tebuconasole WDG and 10% ZJ0712 JG could effectively control wheat scab, and the control effects of one time or two times application were better than control agent carbendasim; the control efficacy of two times application was improved in different degrees against one time ap- vlication. [ Conclusion] The naner vrevided basis for the control atminst wheat scab.
文摘The cucumber scab caused by Cladosporium cucumerinum Ell. is one of the most important diseases of cucumber in the PR. of China and elsewhere. Recently, wider acceptance of under glas culture exacerbates the problem. Both in greenhouses and in free fields in the Northern China pose a challenge in the control of this disease, because there are no effective fungicide to be used. Induced resistance with chemicals may be an ideal way to protect cucumber against this disease , if there are no resistant cultivars to be used. This paper reports the effect of BION, a new activator of SAR, on the induction of resistance to cucumber scab using two treatment methods (seed treatment and foliar spray) at different concentrations (10,20,40, 80, 160 mg. mL- 1). The results showed that the number of diseased plants was decreased drastically in BION - treated plants, the disease incidence was 35 %, whereas it was 75 % in control plants. The disease severity of plant with BION treatment by foliar spray was 2 and it was 1.5 if used as seed treatment, whereas it was 3 and 3.1 in control plants respectively. Moreover it has no phytotoxic effect to be observed at concentrations used in the experiment.
基金collectively funded by the USDA hatch projects SD00H695-20,USDA-ARS agreement 59-0206-0-177(USDAUSWBSI)the Agriculture and Food Research Initiative Competitive Grant 2022-68013-36439(Wheat CAP)from the USDA National Institute of Food and AgricultureSouth Dakota Wheat Commission Grant 3X1340。
文摘Fusarium head blight(FHB),also known as scab,is a devastating fungal disease of wheat that causes significant losses in grain yield and quality.Quantitative inheritance and cumbersome phenotyping make FHB resistance a challenging trait for direct selection in wheat breeding.Genomic selection to predict FHB resistance traits has shown promise in several studies.Here,we used univariate and multivariate genomic prediction models to evaluate the prediction accuracy(PA)for different FHB traits using 476elite and advanced breeding lines developed by South Dakota State University hard winter wheat breeding program.These breeding lines were assessed for FHB disease index(DIS),and percentage of Fusarium damaged kernels(FDK)in three FHB nurseries in 2018,2019,and 2020(TP18,TP19,and TP20)and were evaluated as training populations(TP)for genomic prediction(GP)of FHB traits.We observed a moderate PA using univariate models for DIS(0.39 and 0.35)and FDK(0.35 and 0.37)using TP19 and TP20,respectively,while slightly higher PA was observed(0.41 for DIS and 0.38 for FDK)when TP19 and TP20(TP19+20)were combined to leverage the advantage of a large training population.Although GP with multivariate approach including plant height and days to heading as covariates did not significantly improve PA for DIS and FDK over univariate models,PA for DON increased by 20%using DIS,FDK,DTH as covariates using multi-trait model in 2020.Finally,we used TP19,TP20,and TP19+20 in forward prediction to calculate genomic-estimated breeding values(GEBVs)for DIS and FDK in preliminary breeding lines at an early stage of the breeding program.We observed moderate PA of up to 0.59 for DIS and 0.54for FDK,demonstrating the promise in genomic prediction for FHB resistance in earlier stages using advanced lines.Our results suggest GP for expensive FHB traits like DON and FDK can facilitate the rejection of highly susceptible materials at an early stage in a breeding program.
文摘Based on the survey of observation, the factors afffecting prevalence degree of wheat scab ( Gibberella zeae) were analyzed through coupling method. The results showed that the average diseased ear rate before harvest (disease index) was associated with the average relative humidity from April to early May, the average temperature from April to early May, and the interval from peak maturity stage of perithecium to full heading stage of wheat. The interval was used to measure the coincidence degree between release period of ascospore and ear period ( heading to grouting stage). The prevalence of wheat scab was determined by coincidence degree and meteorological conditions of ear period. Quantitative prediction should be conducted, to improve prediction and control levels.
文摘The research was focused on the persistence of resistance of V. inaequalis to strobilurins from resistant populations occurring in the field. It was studied with two types of experiments: the first in apple orchards where resistance occurred in 2005 and employ of these fungicides was suspended from 2006 to 2011, realizing every year sensitivity/resistance assays on strobilurins. In the second was verified the sensitivity/resistance of the same 2015 populations, repeatedly multiplied (ten inoculations) in glasshouse on apple seedlings. Results showed that the resistance survived in orchards all the years of tests (six years), despite sensitivity fluctuations occurred on infected leaves due to sexual crosses, competition with sensible strains and soil management. In glasshouse, the level of resistance of all populations increased quickly in first inoculations and values remained high (EC50 > 10 mg·L-1) in all the following inoculations, until the last, the 10th. V. inaequalis resistant strains seemed to show high fitness: relevant persistence in the field, and a capacity of prevail on sensible ones in absence of factors of field variability.
文摘Dithianon is a multi-site fungicide and has never been object of suspects and reports of reduced sensitivity and activity. Italian IFP technicians had the suspect of reductions of activity by this fungicide on </span><i><span style="font-family:Verdana;">Venturia inaequalis.</span></i><span style="font-family:Verdana;"> Methodologies, </span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> and </span><i><span style="font-family:Verdana;">in vivo</span></i><span style="font-family:Verdana;"> were carried out to verify this suspect. Populations poorly controlled with suspects on dithianon and sensible ones </span><span><span style="font-family:Verdana;">were utilized. The tests </span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> permitted to evidence light and non-significant</span></span><span style="font-family:Verdana;"> reductions of sensitivity of poorly controlled populations </span></span><span style="font-family:Verdana;">with </span><span style="font-family:Verdana;">respect </span><span style="font-family:Verdana;">to </span><span style="font-family:""><span style="font-family:Verdana;">sensible ones. </span><i><span style="font-family:Verdana;">In vivo tests</span></i><span style="font-family:Verdana;"> on seedlings were non</span></span><span style="font-family:Verdana;">-</span><span style="font-family:""><span style="font-family:Verdana;">reliable for a general low activity of dithianon. On the contrary, the original </span><i><span style="font-family:Verdana;">in vivo</span></i><span style="font-family:Verdana;"> methodology on grafted apple plants showed several reductions of activity, with moderate levels and a spot distribution in orchards. The cause was probably due to the increase</span></span><span style="font-family:Verdana;">d</span><span style="font-family:Verdana;"> treatments with dithianon caused by problems on other groups of fungicides and by a high infective pressure in some years. It is discussed if this reduction can be considered a resistance phenomenon or a temporary modification of the interactions </span><span style="font-family:Verdana;">of </span><span style="font-family:Verdana;">plant-fungus-fungicide.
文摘Among the most economically important fruit crops in South and North Kyrgyzstan affected by scab disease are apples (Malus domestica). Natural isolates of Venturia inaequalis were isolated from diseased parts of apple trees, selected from different regions, through sedimentation of air microflora on the crowns of fruit trees. Two biocontrol agents as Trichoderma viride and Streptomyces sр. were tested for apple scab control in vitro and field conditions. Two applications of Trichoderma viride within 35 days completely stopped the scab disease in seedling leaves. Within 40 days, the seedlings were recovered;the new leaves have blossomed on branches. The Streptomyces sp. antagonists were inferior in effectiveness compared to Trichoderma viride;nevertheless, they prevented progression of the disease in the leaves. Seedlings grew new shoots and leaves around 50 days after the second treatment. Orchard experiment results provide great hope that a biological product based on Trichoderma viride could work as an effective agent to suppress the development of the pathogen in the early spring, when leaves start to show scab disease symptoms.
文摘Biological methodologies (</span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> and </span><i><span style="font-family:Verdana;">in vivo</span></i><span style="font-family:Verdana;">) were tested on </span><i><span style="font-family:Verdana;">Venturia inaequalis</span></i><span style="font-family:Verdana;"> to assay sensitivity of populations treated with the SDHI penthiopyrad, fluxapyroxad and fluopyram that showed a field poor control in an experimental Centre. In comparison, sensible populations were tested. </span><i><span style="font-family:Verdana;">I</span></i><i><span style="font-family:Verdana;">n vitro</span></i><span style="font-family:Verdana;"> trials, the poorly controlled populations presented moderate increases of EC</span><sub><span style="font-family:Verdana;">50</span></sub><span style="font-family:Verdana;"> and RF values in all three years in which tests were carried out. The </span><i><span style="font-family:Verdana;">in vivo</span></i><span style="font-family:Verdana;"> trials in glasshouse showed that the activity of SDHI fungicides on field poorly controlled populations was highly reduced. These populations can be defined as “resistant” as the high loss of sensitivity and activity were repeatedly assessed in field and glasshouse. Trials showed that these reductions were manifested by curative applications and not by preventive ones, as already ascertained for </span><i><span style="font-family:Verdana;">V. inaequalis </span></i><span style="font-family:Verdana;">populations resistant to AP and DMI fungicides. </span><span><span style="font-family:Verdana;">The cross-resistance of resistant populations to the three SDHIs was highly positive among them. In bibliography, there are only researches of cross-resistance among SDHIs starting from isolates resistant to boscalid, with results of different sensitivities and cross-resistance. These results with populations of </span><i><span style="font-family:Verdana;">V. inaequalis</span></i><span style="font-family:Verdana;"> resistant to the three SDHI offer practical evaluations in cross-resistance.
文摘Little is written on the management of an innocuouslooking skin scab over an autogenous arteriovenous fistula(AVF)used for haemodialysis.The seriousness of the underlying pathology can be under-estimated,and this may lead to early loss of the AVF,and majorlife-threatening haemorrhage.We describe the management of a 78-year-old patient presenting with an innocuous-looking scab over an AVF and review the pertinent literature on this subject.