[ Objective] The paper was to observe Helicotylenchus digonicus by scanning electron microscope （SEM）. [ Method ] H. digonicus collected from Changxing of Zhejiang Province was observed under scanning electron micro...[ Objective] The paper was to observe Helicotylenchus digonicus by scanning electron microscope （SEM）. [ Method ] H. digonicus collected from Changxing of Zhejiang Province was observed under scanning electron microscope, and its morphological structure was confmned under optical microscope. [ Resuit]The nematode specimens fixed by glutaraldehyde and osmium tetroxide and prepared by critical point drying were unmodified and unshrinkable with clear mor- phological structure, which could be scanned very well by scanning electron microscope. [ Conclusion ] The study provided reference for study and control of H. digonicus.展开更多
Ore grades are currently declining and mines are increasingly required to operate more efficiently in order to save costs and conserve resources. As a result, automated procedures to control and measure process effici...Ore grades are currently declining and mines are increasingly required to operate more efficiently in order to save costs and conserve resources. As a result, automated procedures to control and measure process efficiency have become popular: The demand for sustainable mining and metals processing is globally increasing. Mineral Liberation Analysis is a well established technique to analyse and optimize mining processes and its significance to process mineralogy studies and mineral & metallurgical processing has been widely reported. Dedicated systems consisting of an optimized scanning electron microscopes (SEM) and usually several X-ray detectors are an established solution. With the advent of large area SDDs (Silicon drift detector) and progress in stage automation on conventional SEMs, the speed of X-Ray acquisition is no longer a limiting factor. However, until now, the particle analysis packages on commercially available systems do not lend themselves to Mineral Liberation Analysis without significant modification. INCAMineral is a new solution for mineral liberation analysis which runs on conventional SEMs equipped with large area SDDs.展开更多
The utility of GIS （geographic information system） methods and spatial statistical analysis on spectral maps of sediment samples were examined. Detailed elemental maps are often constructed using energy dispersive X...The utility of GIS （geographic information system） methods and spatial statistical analysis on spectral maps of sediment samples were examined. Detailed elemental maps are often constructed using energy dispersive X-ray techniques and SEM （scanning electron microscopy）. The elemental neighborhood associations of a single element, P （phosphorus）, were quantified at a magnification of 3,000 ×. For each of the 170,000 pixels on the images which displayed a strong P concentration, neighborhoods from 0.1μm^2 to 12 μm^2 were examined for associated elemental concentrations. PCA （principal component analysis） revealed two significant neighborhood types associated with P in samples of pH 4, and three neighborhood types at pH 8. These neighborhoods corresponded to Mg-P associations commonly found to be chemically prevalent in river sediments impacted by agricultural operations. Discriminant analysis showed that the greatest accuracy in predicting sample pH could be achieved by using a neighborhood size of 12 ~m2. Potassium at relatively large neighborhood sizes was the element most significant in predicting pH. While many of the chemical associations in close proximity to P could be predicted and explained through mineral solubility, spatial analysis provided some interesting insights into the structure of the samples. Results also indicted differences in the spatial scale associated with different processes.展开更多
This study shows that submicron/nanoparticles found in bacterial cells (S. aureus) incubated with polyurethane (a material commonly used for prostheses in odontostomatology) are a consequence of biodestruction. The pr...This study shows that submicron/nanoparticles found in bacterial cells (S. aureus) incubated with polyurethane (a material commonly used for prostheses in odontostomatology) are a consequence of biodestruction. The presence of polyurethane nanoparticles into bacterial vesicles suggests that the internalization process occurs through endocytosis. TEM and FIB/SEM are a suitable set of correlated instruments and techniques for this multi facet investigation: polyurethane particles influence the properties of S. aureus from the morpho-functional standpoint that may have undesirable effects on the human body. S. aureus and C. albicans are symbiotic microorganisms;it was observed that C. albicans has a similar interaction with polyurethane and an increment of the biodestruction capacity is expected by its mutual work with S. aureus.展开更多
膨润土作为缓冲或回填材料,具有吸水性极强、膨胀性较大、渗透性极低等特性。膨润土在饱和状态与非饱和状态之间转换时,因其湿胀干缩产生的裂缝会导致工程屏障受到破坏。因此,对具有高膨胀性的膨润土的持水特性及微观结构特征进行试验...膨润土作为缓冲或回填材料,具有吸水性极强、膨胀性较大、渗透性极低等特性。膨润土在饱和状态与非饱和状态之间转换时,因其湿胀干缩产生的裂缝会导致工程屏障受到破坏。因此,对具有高膨胀性的膨润土的持水特性及微观结构特征进行试验研究显得非常必要。分别运用滤纸法和饱和盐溶液蒸汽平衡法对膨润土进行了持水特性试验研究,得到了不同吸力范围内膨润土的土-水特征曲线;联合用蒸汽平衡法和扫描电子显微镜(SEM)对特定吸力点(367.54、149.51、71.12、38.00 MPa)的膨润土试样进行扫描电镜试验研究。膨润土的持水特性试验结果表明,滤纸法和饱和盐溶液蒸汽平衡法测得的土-水特征曲线均随吸力的增大而减小。根据滤纸法量测的试验数据,运用origin软件获得了Fredlund and Xing(1994)提出的模型参数,通过建立模型参数与干密度之间的关系,给出了膨润土的土-水特征曲线的预测公式。膨润土的微观定性分析表明:随着吸力的增大,膨润土集聚体逐渐增大,膨润土颗粒之间也越紧密;孔隙数量随着吸力的增大而减少、孔径也随吸力的增大而减小。展开更多
[ Objective] The research aimed to explore the manufacturing methods of scanning electron microscope （SEM） and transmission electron microscopy （TEM） for oocyte and provide technical support for related research. ...[ Objective] The research aimed to explore the manufacturing methods of scanning electron microscope （SEM） and transmission electron microscopy （TEM） for oocyte and provide technical support for related research. [ Method] Based on GV-and MII-stage oocytes, samples of SEM and TEM were prepared respectively, then ultrastructure changes were observed. [ Result] The results showed that the method needed few samples, keep intact cell morphology and can see clear ultrastructure. [Conclusion] The method is suitable for ultrastructural observation of oocyte.展开更多
文摘[ Objective] The paper was to observe Helicotylenchus digonicus by scanning electron microscope （SEM）. [ Method ] H. digonicus collected from Changxing of Zhejiang Province was observed under scanning electron microscope, and its morphological structure was confmned under optical microscope. [ Resuit]The nematode specimens fixed by glutaraldehyde and osmium tetroxide and prepared by critical point drying were unmodified and unshrinkable with clear mor- phological structure, which could be scanned very well by scanning electron microscope. [ Conclusion ] The study provided reference for study and control of H. digonicus.
文摘Ore grades are currently declining and mines are increasingly required to operate more efficiently in order to save costs and conserve resources. As a result, automated procedures to control and measure process efficiency have become popular: The demand for sustainable mining and metals processing is globally increasing. Mineral Liberation Analysis is a well established technique to analyse and optimize mining processes and its significance to process mineralogy studies and mineral & metallurgical processing has been widely reported. Dedicated systems consisting of an optimized scanning electron microscopes (SEM) and usually several X-ray detectors are an established solution. With the advent of large area SDDs (Silicon drift detector) and progress in stage automation on conventional SEMs, the speed of X-Ray acquisition is no longer a limiting factor. However, until now, the particle analysis packages on commercially available systems do not lend themselves to Mineral Liberation Analysis without significant modification. INCAMineral is a new solution for mineral liberation analysis which runs on conventional SEMs equipped with large area SDDs.
文摘The utility of GIS （geographic information system） methods and spatial statistical analysis on spectral maps of sediment samples were examined. Detailed elemental maps are often constructed using energy dispersive X-ray techniques and SEM （scanning electron microscopy）. The elemental neighborhood associations of a single element, P （phosphorus）, were quantified at a magnification of 3,000 ×. For each of the 170,000 pixels on the images which displayed a strong P concentration, neighborhoods from 0.1μm^2 to 12 μm^2 were examined for associated elemental concentrations. PCA （principal component analysis） revealed two significant neighborhood types associated with P in samples of pH 4, and three neighborhood types at pH 8. These neighborhoods corresponded to Mg-P associations commonly found to be chemically prevalent in river sediments impacted by agricultural operations. Discriminant analysis showed that the greatest accuracy in predicting sample pH could be achieved by using a neighborhood size of 12 ~m2. Potassium at relatively large neighborhood sizes was the element most significant in predicting pH. While many of the chemical associations in close proximity to P could be predicted and explained through mineral solubility, spatial analysis provided some interesting insights into the structure of the samples. Results also indicted differences in the spatial scale associated with different processes.
文摘This study shows that submicron/nanoparticles found in bacterial cells (S. aureus) incubated with polyurethane (a material commonly used for prostheses in odontostomatology) are a consequence of biodestruction. The presence of polyurethane nanoparticles into bacterial vesicles suggests that the internalization process occurs through endocytosis. TEM and FIB/SEM are a suitable set of correlated instruments and techniques for this multi facet investigation: polyurethane particles influence the properties of S. aureus from the morpho-functional standpoint that may have undesirable effects on the human body. S. aureus and C. albicans are symbiotic microorganisms;it was observed that C. albicans has a similar interaction with polyurethane and an increment of the biodestruction capacity is expected by its mutual work with S. aureus.
文摘膨润土作为缓冲或回填材料,具有吸水性极强、膨胀性较大、渗透性极低等特性。膨润土在饱和状态与非饱和状态之间转换时,因其湿胀干缩产生的裂缝会导致工程屏障受到破坏。因此,对具有高膨胀性的膨润土的持水特性及微观结构特征进行试验研究显得非常必要。分别运用滤纸法和饱和盐溶液蒸汽平衡法对膨润土进行了持水特性试验研究,得到了不同吸力范围内膨润土的土-水特征曲线;联合用蒸汽平衡法和扫描电子显微镜(SEM)对特定吸力点(367.54、149.51、71.12、38.00 MPa)的膨润土试样进行扫描电镜试验研究。膨润土的持水特性试验结果表明,滤纸法和饱和盐溶液蒸汽平衡法测得的土-水特征曲线均随吸力的增大而减小。根据滤纸法量测的试验数据,运用origin软件获得了Fredlund and Xing(1994)提出的模型参数,通过建立模型参数与干密度之间的关系,给出了膨润土的土-水特征曲线的预测公式。膨润土的微观定性分析表明:随着吸力的增大,膨润土集聚体逐渐增大,膨润土颗粒之间也越紧密;孔隙数量随着吸力的增大而减少、孔径也随吸力的增大而减小。
基金Supported by Natural Science Foundation of Jiangsu Province(Grant number:BK2008589)Shanghai Committee(Grant num-ber:2003 #14-1)~~
文摘[ Objective] The research aimed to explore the manufacturing methods of scanning electron microscope （SEM） and transmission electron microscopy （TEM） for oocyte and provide technical support for related research. [ Method] Based on GV-and MII-stage oocytes, samples of SEM and TEM were prepared respectively, then ultrastructure changes were observed. [ Result] The results showed that the method needed few samples, keep intact cell morphology and can see clear ultrastructure. [Conclusion] The method is suitable for ultrastructural observation of oocyte.
