Non-alcoholic fatty liver disease(NAFLD),characterized by hepatic steatosis,is a common metabolic liver disease worldwide.Currently,satisfactory drugs for NAFLD treatment remain lacking.Obesity and diabetes are the le...Non-alcoholic fatty liver disease(NAFLD),characterized by hepatic steatosis,is a common metabolic liver disease worldwide.Currently,satisfactory drugs for NAFLD treatment remain lacking.Obesity and diabetes are the leading causes of NAFLD,and compounds with anti-obesity and antidiabetic activities are considered suitable candidates for treating NAFLD.In this study,biochemical and histological assays revealed that a natural lignan schisanhenol(SAL)effectively decreased lipid accumulation and improved hepatic steatosis in free fatty acid(FFA)-treated HepG2 cells and high-fat diet(HFD)-induced NAFLD mice.Further,molecular analyses,microRNA(miRNA)-seq,and bioinformatics analyses revealed that SAL may improve NAFLD by targeting the miR-802/adenosine monophosphateactivated protein kinase(AMPK)pathway.Liver-specific overexpression of miR-802 in NAFLD mice significantly impaired SAL-mediated liver protection and decreased the protein levels of phosphorylated(p)-AMPK and PRKAB1.Dual-luciferase assay analysis further confirmed that miR-802 inhibits hepatic AMPK expression by binding to the 3’untranslated region of mouse Prkab1 or human PRKAA1.展开更多
Objective: To explore the effects of Schisanhenol (Sal) on generation of reactive oxygen species (ROS) including superoxide anion, hydrogen peroxide and hydroxyl radical as well as on the activity of superoxide dismut...Objective: To explore the effects of Schisanhenol (Sal) on generation of reactive oxygen species (ROS) including superoxide anion, hydrogen peroxide and hydroxyl radical as well as on the activity of superoxide dismutase (SOD) and catalase in respiratory burst of rat neutrophils.Methods: Rat neutrophils were prepared from male Wistar rats. Superoxide anion, hydrogen peroxide, hydroxyl radical, SOD and catalase activity were measured.Results: Sal (1, 10, 100 μmol/L) inhibited generation of ROS of rat neutrophils during respiratory burst induced by formyl-methionyleucylphenylalanine (FMLP) and phorbol myristate acetate (PMA) dosedependently, and it enhanced SOD and catalase activities.Conclusion: Sal may inhibit generation of ROS and enhance activity of anti-oxidant enzymes of neutrophils to reduce oxidative injury to tissues.展开更多
基金National Natural Science Foundation of China(Nos.82304708 and U23A20511)the CAMS Innovation Fund for Medical Sciences(2023-I2M-QJ-013,2021-I2M-1-031,2022-I2M-2-002,and 2023-I2M-3-006,China)the Beijing Natural Science Foundation(No.Z220019,China).
文摘Non-alcoholic fatty liver disease(NAFLD),characterized by hepatic steatosis,is a common metabolic liver disease worldwide.Currently,satisfactory drugs for NAFLD treatment remain lacking.Obesity and diabetes are the leading causes of NAFLD,and compounds with anti-obesity and antidiabetic activities are considered suitable candidates for treating NAFLD.In this study,biochemical and histological assays revealed that a natural lignan schisanhenol(SAL)effectively decreased lipid accumulation and improved hepatic steatosis in free fatty acid(FFA)-treated HepG2 cells and high-fat diet(HFD)-induced NAFLD mice.Further,molecular analyses,microRNA(miRNA)-seq,and bioinformatics analyses revealed that SAL may improve NAFLD by targeting the miR-802/adenosine monophosphateactivated protein kinase(AMPK)pathway.Liver-specific overexpression of miR-802 in NAFLD mice significantly impaired SAL-mediated liver protection and decreased the protein levels of phosphorylated(p)-AMPK and PRKAB1.Dual-luciferase assay analysis further confirmed that miR-802 inhibits hepatic AMPK expression by binding to the 3’untranslated region of mouse Prkab1 or human PRKAA1.
文摘Objective: To explore the effects of Schisanhenol (Sal) on generation of reactive oxygen species (ROS) including superoxide anion, hydrogen peroxide and hydroxyl radical as well as on the activity of superoxide dismutase (SOD) and catalase in respiratory burst of rat neutrophils.Methods: Rat neutrophils were prepared from male Wistar rats. Superoxide anion, hydrogen peroxide, hydroxyl radical, SOD and catalase activity were measured.Results: Sal (1, 10, 100 μmol/L) inhibited generation of ROS of rat neutrophils during respiratory burst induced by formyl-methionyleucylphenylalanine (FMLP) and phorbol myristate acetate (PMA) dosedependently, and it enhanced SOD and catalase activities.Conclusion: Sal may inhibit generation of ROS and enhance activity of anti-oxidant enzymes of neutrophils to reduce oxidative injury to tissues.
