Construction of a hepatic stellate cells subtracted cDNA library of differentially expressed genes in normal mice and mice with Schistosomiasis japonica

To construct a hepatic stellate cells (HSCs) subtracted cDNA library to find differentially expressed genes in normal mice and mice infected with Schistosoma japonicum (S. japonicum). Suppression subtractive hybridization (SSH) was used. The cDNA fragments of normal mouse were compared to those of schistosoma-infected mice to find differentially expressed genes. Then differentially expressed cDNA fragments were directly inserted into T/A cloning vector to set up the subtractive library. Amplification of the library was carried out with transformation of DH5α. The amplified library contained more than 400 positive bacterial clones, which were then hybridized with forward and backward subtracted probes for differential screening. One hundred positive bacterial clones were randomly selected for sequencing and BLAST analysis. Finally, virtual Northern Blot confirmed such differential expression. The subtracted cDNA library of differentially expressed genes of HSCs was constructed successfully, the library is efficient and lays foundation for screening and cloning new and specific genes of schistosomiasis.

The Role of TGF-β_1 in Mice Hepatic Fibrosis by Schistosomiasis Japonica

To investigate the role of transforming growth factor-β1 (TGF-β1) in mice with hepatic fi- brosis caused by Schistosomiasis Japonica, ELISA,VG staining and multimedia color hieroglyph quan- titative analysis were used to study the change of the serum TGF-β1, liver collagen fiber and reticular fiber in mice. The level of serum TGF-β1 in experimental group was significantly higher than that in control group (P<0.01 or P<0. 05) 8, 10, 12 weeks after infected by schistosomiasis. After infec- tion, the level of liver collagen fiber and reticular fiber, and that of TGF -β1 increased over time (P< 0.01 or P<0. 05). In mice infected by Schistosomiasis Japonica, the level of TGF-β1 increased with prolongation of infection time, and with the increase of liver collagen fiber and reticular fiber. TGFβ1 plays an important role of immunomodulation in hepatic fibrosis formation caused by Schistosomiasis Japonica.

Publication output of the new integrated strategy for schistosomiasis japonica control in China: a PubMed-based bibliometric assessment

Background:Although sustainable control since 1950s has achieved great successes,schistosomiasis japonica remains a major public health problem in China.Since 2004,a new integrated strategy was developed aiming to control the transmission of Schistosoma japonicum through the implementation of a package of interventions.To date,no systematic review or meta-analysis assessing the effectiveness of this new integrated strategy for schistosomiasis control in China has been published.We performed a PubMed-based bibliometric assessment of publications on the new integrated strategy for schistosomiasis japonica control in China,to understand the global transmissibility and sharing of the new integrated strategy.Methods:An in-depth bibliometric analysis of all publications on the new integrated strategy for schistosomiasis japonica control in China was performed through a PubMed search using the terms 'schistosomiasis' and 'China,'from January 1,2004 to August 31,2018.All titles and abstracts were read carefully,and the publications reporting the effectiveness,experiences,lessons,or problems of the new integrated strategy were included in the bibliometric analysis.Results:Overall,2,361 titles were screened,and 70 eligible publications were accessed for analyses,including 23 studies in English,published in 15 international journals,and 47 studies in Chinese with abstracts in English,published in 3 national journals.Chinese Journal of Schistosomiasis Control (Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi) published 60% of the research output,Research articles (48.6%) and short reports (37.1%) were the dominant manuscript types.Furthermore,471 contributing authors from 277 affiliations across 9 countries produced these 70 publications.Conclusion:This is the first PubMed-based quantitative analysis of the research output of the new integrated strategy,and our data indicate a low global transmissibility of Chinese new integrated strategy.We therefore call for more research outputs of the new integrated strategy for schistosomiasis japonica control in China to be communicated through international platforms.

Study on IL-4, IL-5 and IL-10 in Liver and Bone Marrow of Mice Infected with Schistosomiasis Japonica

Summary: To compare Th2 cytokines levels in livers and bone marrow of mice infected with Schistosomiasis Japonica and understand their implication, ABC immunohistochemical staining technique and multimedia pathologic pictures analysis were used to detect the IL 4, IL 5 and IL 10 derived from livers and bone marrow of infected mice. Results showed that in the livers of infected mice, IL 4, IL 5 and IL 10 were obviously increased over time. The level of IL 4 was increased most among the 3 indicators. In bone marrow of infected mice, however, even though IL 4 was slowly increased over time, it was lower than that of livers in 10th and 12th week. The level of IL 5 was higher than that of livers during 12th weeks. Before 10th weeks IL 10 was elevated. After 12th week IL 10 was decreased and obviously lower than that of livers. It is concluded that in mice infected with Schistosomiasis Japonica , the immune response occurred mainly in the liver. Therefore, in bone marrow, IL 5 showed obvious auto secretion. It might be related to its biological ability to induce B cell division and eosinophil differentiation.

Dynamic changes in the collagen metabolism of liver fibrosis at the transcription level in rabbits with Schistosomiasis japonica

OBJECTIVE: To study the role of the synthesis and degradation of collagen at the transcription level during liver fibrogenesis due to schistosomiasis japonica in rabbits. METHODS: New Zealand rabbits challenged by cercariae of Schistosoma japonicum (S. japonicum) were served as animal models for liver fibrosis. Liver specimens were collected through operations at 4, 6, 8, 10, 12, 16, 20, 24 and 28 wks after challenge. Type I collagen, type III collagen, type IV collagen, MMP-1 and MMP-9 mRNA levels of liver tissue were detected by RT-PCR + Dot blot. The size of egg granulomas and the degree of liver fibrosis were measured by histopathological examinations. RESULTS: Type I collagen, type III collagen, type IV collagen, MMP-1 and MMP-9 mRNA levels increased simultaneously in the early stage after challenge. Most of them reached their peak at 10 weeks, and compared with normal controls, type I collagen, type III collagen, type IV collagen, MMP-1 and MMP-9 mRNA levels increased by 12.0-, 11.0-, 6.6-, 10.0- and 11.0-fold, respectively, coinciding with the change of egg granulomas, i.e., the change in the inflammatory process. Then both collagen and collagenase mRNA levels decreased. Type I, III and IV collagen mRNA levels declined to 2-fold to 3-fold as compared with normal controls (P 0.05) at 28 wks. This study shows that the synthesis and degradation of collagen keep a dynamic balance at the early stage of schistosomiasis japonica challenge, while at the later stages the quantity of collagen synthesis was higher than that of collagen degradation. CONCLUSIONS: It was confirmed at transcription level that when the quantity of collagen synthesis was higher than that of collagen degradation liver fibrogenesis may be resulted in.

Interruption of schistosomiasis transmission in mountainous and hilly regions with an integrated strategy:a longitudinal case study in Sichuan,China

Background:Schistosomiasis remains a major public health concern in China.Since 2004,an integrated strategy was developed to control the transmission of Schistosoma japonicum in China.However,the long-term effectiveness of this integrated strategy for the interruption of schistosomiasis transmission remains unknown in the mountainous and hilly regions of China until now.This longitudinal study aims to evaluate the effectiveness of the integrated strategy on transmission interruption of schistosomiasis in Sichuan Province from 2005 through 2014.Methods:The data regarding replacement of bovines with machines,improved sanitation,access to clean water,construction of public toilets and household latrines,snail control,chemotherapy,and health education were captured from the annual report of the schistosomiasis control programmes in Sichuan Province from 2005 to 2014,and S.japonicum infection in humans,bovines and snails were estimated to evaluate the effectiveness of the integrated strategy.Results:During the 10-year period from 2005 through 2014,a total of 536568 machines were used to replace bovines,and 3284333 household lavatories and 15523 public latrines were built.Tap water was supplied to 19116344 residents living in the endemic villages.A total of 230098 hm2 snail habitats were given molluscicide treatment,and 357233 hm2 snail habitats received environmental improvements.There were 7268138 humans and 840845 bovines given praziquantel chemotherapy.During the 10-year study period,information,education and communication(IEC)materials were provided to village officers,teachers and schoolchildren.The 10-year implementation of the integrated strategy resulted in a great reduction in S.japonicum infection in humans,bovines and snails.Since 2007,no acute infection was detected,and no schistosomiasis cases or infected bovines were identified since 2012.In addition,the snail habitats reduced by 62.39%in 2014 as compared to that in 2005,and no S.japonicum infection was identified in snails since 2007.By 2014,88.9%of the endemic counties achieved the transmission interruption of schistosomiasis and transmission control of schistosmiasis was achieved in the whole province in 2008.Conclusion:The government-directed and multi-department integrated strategy is effective for interrupting the transmission of schistosomiasis in the mountainous and hilly regions of China.

Estimating the prevalence of schistosomiasis japonica in China:a serological approach

Background:The prevalence of schistosomiasis japonica has decreased significantly,and the responses changing from control to elimination in Jiangsu Province,P.R.China.How to estimate the change in prevalence of schistosomiasis using only serological data will be important and useful.Methods:We collected serum samples from 2011 to 2015 to build a serum bank from Dantu County of Jiangsu,China.Serum samples were detected by enzyme-linked immunosorbent assay(ELISA),the positive rate and optical density(OD)value were obtained.The Bayesian model including the prior information of sensitivity and specificity of ELISA was established,and the estimated infection rates were obtained for different years,genders and age groups.Results:There was no significant difference in the mean OD between different years and genders,but there was a significant difference between the different age groups.There were statistically significant differences in the positive rate for different years and age groups,but no significant difference at different genders.The estimated infection rate for the five years was 1.288,1.456,1.032,1.485 and 1.358%,respectively.There was no significant difference between different years and between genders,but a significant difference between different age groups.Conclusions:The risk of schistosomiasis transmission in this area still exists,and risk monitoring of schistosomiasis should be strengthened.

Factors associated with coverage of praziquantel for schistosomiasis control in the communitydirect intervention (CDI) approach in Mali (West Africa)

Background:Despite the progress made in the control of Neglected Tropical Diseases(NTD),schistosome and soil-transmitted helminth infections are far from being effectively managed in many parts of the world.Chemotherapy,the key element of all control strategies,is faced with some difficulties in terms of access to treatment.Our study aims to describe the factors involved in the success or failure of the community-directed intervention(CDI)approach through control programmes,which aims to achieve consistent high coverage at affordable and sustainable costs in endemic areas.Methods:The CDI approach was carried out from December 2007 to October 2008 in ten villages of the district of Diéma,Mali.At inclusion,each child part of the study’s sample was interviewed and submitted for a physical examination.The study focused on:data collection,treatment of the eligible population,evaluation of the treatment coverage,performance of community drug distributors(CDDs),and the involvement and perception of populations.Results:A total of 8,022 eligible people were studied with a mean coverage rate of 76.7%.Using multiple regression,it was determined that receiving praziquantel as treatment was associated with five factors:belonging to the Fulani or Moorish ethnic minority versus the Bambara/Soninke,use of the central versus the house-to-house drug distribution mode,the ratio of the population to the number of CDDs,the lack of supervision and belonging to the age group of 15 years or above(p<0.05).As well as that,it was found that the presence of parallel community-based programmes(HIV,tuberculosis)that provide financial incentives for community members discouraged many CDDs(who in most cases are volunteers)to participate in the CDI approach due to a lack of incentives.Conclusions:The findings indicate that the success of the CDI approach depends on,amongst other things,the personal characteristics of the respondents,as well as on community factors.

Modulation of in vivo granuloma formation related to regulation of in vitro IFN γ and IL 4 expressions in experimental Schistosomiasis japonica

Objective To find out relationship between the in vitro schistosome egg antigen (SEA) stimulated IFN γ and IL 4 mRNA expressions and the in vivo SEA elicited granulomatous responses, the transcriptions of IFN γ and IL 4 gene were investigated in the spleen of BALB/c mice infected with Schistosoma japonicum (S japonicum) Methods Spleens were removed at 0, 3, 5, 8, and 10 and 12 weeks after infection and the spleen cells were incubated in the presence of SEA The extracted RNA was analyzed for IFN γ and IL 4 mRNA by reverse transcription PCR (RT PCR) Newly formed liver granulomas were measured Results The study revealed that no detectable IFN γ and IL 4 mRNA RT PCR products were found in SEA treated spleen cells from uninfected, or 3 week infected mice, whereas IL 4 mRNA was found to be expressed in 5 and 8 week infected mice, and an appreciable enhanced expression of IL 4 mRNA was observed in SEA stimulated spleen cells at 8 week infection than at 5 week infections However, SEA could not induce IFN γ and IL 4 mRNA transcription in 10 and 12 week infected mice, indicating the modulated expression of IFN γ and IL 4 mRNA Conclusion The significant changes of IL 4 mRNA expression in SEA stimulated spleen cells during S japonicum infection were coincident with SEA elicited granuloma formation and modulation in vivo

A novel and effective hyperthermia method for Schistosomiasis japonica prevention and treatment

The emerging resistance to schistosoma has been paid much attention and is in urgent need for a novel strategy to control the prevalent parasitic zoonosis. In this study, the efficiency of hyperthermia therapy was investigated by the skin hyperthermia device. The survival rate of cercariae decreased from 68.15 % （37 ℃, 5 min） to 0 （49℃, 10 min） with the thermal dosages increased, which proved the preventing effect of hyperthermia therapy （P 〈 0.05）. Therapeutic effects were assessed in Schistosoma japonicum-infected BALB/c mice. When the cercarial contact region of the skin was treated at 45-49℃ for 5 min within 8 h of infection, worm reduction rate （WRR） reached 74 %-83 % （P 〈 0.01）. The sensitivity of adult schistosoma to heat was also investigated using microwave intraperitoneal hyperthermia （thermal dosages 42-43℃, 20 min）. The WRR, hepatic shift rates and egg reduction rates were 23.7 %, 40 % and 30 %, respectively, comparing with 80.2 %, 59.6 % and 53.9 % of praziquantel （PZQ）-treated group. Encouraging results have been obtained that hyperthermia can effectively kill schistosomula, especially with the appearance of cercarial dermatitis, while PZQ lacks efficacy against the cercariae. Thus, hyperthermia therapy would show significant benefit in preventing and treatment of schistosoma, especially in the early stage.

Paeoniflorin prevents hepatic fibrosis of Schistosomiasis japonica by inhibiting TGF-β1 production from macrophages in mice

In order to investigate the effect of paeoniflorin(PAE)on hepatic fibrosis of mice with Schistosomiasis japonica in vivo and in vitro,a model of hepatic fibrosis caused by schistosomiasis was established in mice infected with cercariae of Schistosoma japonicum.Then,PAE was orally administered before and after praziquantel treat-ment and both therapeutics were given simultaneously at different time points after the infection.The concentra-tion of serum hyaluronic acid(HA)was determined by radioimmunoassay(RIA).Hepatic granuloma and fib-rosis were evaluated via HE and Masson staining.The expression of α-smooth muscle actin(α-SMA),transform-ing growth factor β1(TGF-β1)and collagen I(Col I)protein was detected by immunohistochemistry.The effect of soluble egg antigen(SEA)and PAE on the pro-duction of TGF-β1 from mouse peritoneal macrophages(PMQs)was investigated by RT-PCR,Western blotting and ELISA.The effect of TGF-β1 in optimum macro-phage-conditioned medium(OPMCM)on the prolifera-tion of hepatic stellate cells(HSCs)and collagen secretion from HSCs with anti-TGF-β1 antibody was explored by MTT assay and ELISA.The results show that PAE could significantly reduce the concentration of serum HA,the size of egg granuloma,the severity of hepatic fibrosis and the expression of a-SMA,TGF-β1 and Col I protein in the pre-treatment group.However,in sim-or post-treatment group,PAE did not have any significant therapeutic effect.TGF-β1 could be secreted from PMQs stimulated by SEA.Meanwhile,the production of TGF-β1 from PMQs could be depressed significantly by PAE in a con-centration-dependent manner.TGF-β1 could promote the proliferation of HSCs and the secretion of collagens.In a word,PAE can prevent hepatic granuloma and fib-rosis caused by schistosomiasis japonica through the inhibition of the secretion of TGF-β1 from PMQs,the proliferation and activation of HSCs and the secretion of collagens from HSCs.

Approaches being used in the national schistosomiasis elimination programme in China:a review

Schistosomiasis japonica,caused by the human blood fluke Schistosoma japonicum,remains a major public health problem in China,although great success has been achieved.The control efforts during the past half-decade,notably the wide implementation of the new integrated strategy with emphasis on control of the source of S.japonicum infection across the country since 2004,has greatly reduced S.japonicum in humans,livestock,and intermediate host Oncomelania hupensis snails,and transmission control of schistosomiasis was achieved in China in 2015.A two-stage roadmap was therefore proposed for schistosomiasis elimination in 2015,with aims to achieve transmission interruption by 2020 and achieve disease elimination by 2025 in the country.During the last two decades,a variety of approaches,which target the epidemiological factors of schistosomiasis japonica have been developed,in order to block the transmission cycle of the parasite.These approaches have been employed in the national or local schistosomiasis control activities,and facilitated,at least in part,the progress of the schistosomiasis elimination programs.Here,we present an approach to control the source of S.japonicum infection,three new tools for snail control,three approaches for detecting and monitoring S.japonicum infection,and a novel model for health education.These approaches are considered to play a great role in the stage moving towards transmission interruption and elimination of schistosomiasis in China.

应用胶体金SEA-DIPSTICK法检测日本血吸虫病血清抗体的研究

目的 建立一种快速、简便、实用的检测日本血吸虫病血清抗体的Dipstick方法。方法 应用胶体金SEA -dip stick法检测日本血吸虫病血清抗体。结果 用该法检测急性血吸虫病血清 2 8人份和慢性血吸虫病血清 2 97人份 ,其阳性检出率分别为 10 0 %和 97 31%。在 5 18例正常人和 5 73例其它 4种寄生虫病人血清中有 0 19%假阳性和 2 4%交叉反应。与Dot-ELISA比较 ,经卡方检验表明两者的敏感性无显著性差异 ,而SEA -dipstick法的特异性较Dot -ELISA强。 结论 胶体金SEA -dipstick法检测日本血吸虫病血清抗体有较高的敏感性和特异性 ,并且方法快速 ,操作简便 ,不需特殊仪器设备 ,稳定性高 ,重复性好 ,具有广泛的应用前景。

Immunodiagnostic efficacy of detection of Schistosoma japonicum human infections in China:a meta analysis

Objective:To assess the diagnostic efficacy of the currently most widely used indirect hemagglutination assay(IHA) and enzyme-linked immunosorbent assay(ELISA) for detection of Schistosoma japonicum human infections.Methods:A comprehensive search was undertaken from China National Knowledge Infrastructure,Wanfang Database.VIP Database,PubMed. Cochrane Library,Science Citation Index Expanded.Proquest,and the inclusion and exclusion criteria were strictly settled.The funnel plot was used to assess the publication bias.Cochran’s Q test was employed to measure the homogeneity between studies,a summary receiver operating characteristic(SROC) curve was used to compare the diagnostic accuracy between the IHA and ELISA qualitatively by means of the Weighted Least Square method,the Ordinary Least Square method and the Robust regression method,and the diagnostic odds ratio(DOR) was drawn to compare the accuracy quantitatively.Results:Out of 785 publications,19 papers were eventually selected for analysis.Literature finality assessment indicated that minor publication bias existed in studies pertaining IHA test,but no bias was found in literatures regarding ELISA test.The heterogeneity test showed a heterogeneity between studies was present(χ~2 =466.07 and 34.67. both P values【0.0001).The areas under the SROC curves of IHA were all higher than that of ELISA test using the three methods(Weighted Least Square method:0.766 vs.0.695.Ordinary Least Square method:0.826 vs.0.741.Robust regression:0.815 vs.0.715).The TPR* values for IHA and EUSA were 0.710.0.759.0.749.and 0.650.0.686 and 0.666.respectively,and OR values were 5.997.9.937.8.893.and 3.432.4.784 and 3.959.respectively.The DOR of IHA was 9.41(95% CI:4.88-18.18).and 4.78(95%CI:3.21-7.13) for ELISA.Conclusions:All above results revealed that the diagnostic performance of IHA is better than that of ELISA.However,taking into account their unsatisfactory diagnostic value in areas with low infection intensity,a search for a better diagnostic test that can be applied in field situations in China should be given high priority.

胶体染料斑点兔疫试验法(DIA)检测血吸虫病人血清抗体的进一步研究

本研究对胶体染料斑点免疫试验法(DIA)检测血吸虫病人抗体的有关因素进行了系统的观察,并对实验方法进行了改进.用改进后的DIA检测288份血吸虫病人血清抗体,其中69份急性病人血清,阳性率为95.7%;110份慢性病人血清阳性率为96.4%;19份晚期病人血清阳性率为47.4%;40份华支睾吸虫病人血清,2份阳性,交叉反应率为5%;50份健康人血清,1份阳性,假阳性率2%.敏感性、特异性与酶联斑点免疫试验法相似.改进后的DLA简单、经济、易行,适于现场应用.

rSj26-Sj32-Immunogold-IgG-Dipstick法用于急性日本血吸虫病患者的诊断价值

目的探讨rSj26-Sj32-Immunogold-IgG-Dipstick法用于急性日本血吸虫病患者的诊断价值。方法利用纯化的rSj26-Sj32融合蛋白和日本血吸虫成虫抗原(SjAWA)建立Immunogold-IgG-Dipstick法,检测急性日本血吸虫病患者血清,并以华支睾吸虫病、卫氏并殖吸虫病、棘球蚴病、乙型肝炎、肺结核患者和健康人血清作对照。结果 rSj26-Sj32检测急性日本血吸虫病患者血清的敏感性为96.00%,特异性为97.67%;SjAWA与华支睾吸虫病、卫氏并殖吸虫病和泡型棘球蚴病患者血清存在不同程度的交叉反应,而rSj26-Sj32则未见交叉反应。结论纯化的rSj26-Sj32融合蛋白是1种较好的免疫诊断抗原。

血吸虫病在多个染病者群体传播的S-DI模型的稳定性分析

主要介绍血吸虫病在多个染病者群体传播的S-DI模型,对于两种不同的发生率产生不同的模型,分别求出其平衡点及疾病爆发的阈值,并且分别判断出无病平衡点和地方病平衡点的局部稳定性,并进行了数值模拟。

胶体金CWSAg-DIPSTICK在检测日本血吸虫病中的应用

目的研究胶体金CWSAg-DIP-STICK在检测日本血吸虫病中的应用。方法以日本血吸虫可溶性虫卵抗原(SEAg)、尾蚴抗原(CeAg)为诊断抗原,采用胶体金CWSAg-DIP-STICK对325例血吸虫病人(其中急性血吸虫病28例,慢性血吸虫病297例),79例肝吸虫病人,157例囊虫病人,184例旋毛虫病人,165例肝炎病人,481例肺结核病人,876例普通就诊者以及518例正常人的临床应用与国内几种常用的血清学诊断方法比较试验。结果胶体金CWSAg-DIPSTICK操作时间为2～5min,阳性检出率为(97.31％～100％)。其中慢性血吸虫病97.31％(289/297),急性血吸虫病100％(28/28);交叉反应率为0.62％～4.89％,其中,肺吸虫病1.96％(3/153),肝吸虫病1.33％(1/75),囊虫病0.64％(3/153),旋毛虫病4.89％(9/184),肝炎1.21％(2/165),结核病0.62％(3/481);假阳性率为0.19％～1.25％,其中,寄生虫病特检门诊普通就诊者1.25％(11/876)、正常人0.19％(1/518)。结论胶体金日本血吸虫病快速诊断试纸具有特异性较强,操作简便,反应快速,敏感性较高,假阳性率低等优点,值得临床上推广应用。

间接血凝试验在日本血吸虫病门诊筛查诊断中的价值分析

目的分析间接血凝试验(IHA)在日本血吸虫病门诊筛查诊断中的效能。方法选取2021年12月至2023年1月于本站行日本血吸虫病筛查的300例居民,实施IHA与胶体金免疫渗滤斑点法(DIGFA)。以病原学检测(改良加藤厚涂片法合并尼龙绢集卵孵化法)为“金标准”,对比分析IHA、DIGFA在日本血吸虫病门诊筛查中的效能。结果以病原学检测为“金标准”,IHA的灵敏度、特异度、准确度分别为80.77%(21/26)、98.20%(273/278)、98.00%(294/300),明显高于DIGFA的23.08%(6/26)、93.13%(271/291)、92.33%(277/300),差异有统计学意义(P<0.05)。κ检验显示,IHA筛查日本血吸虫病与病原学检测结果具有极好的一致性(κ=0.864,P=0.000)。结论IHA在日本血吸虫病诊断中的特异度、准确度较高,可用于日本血吸虫病的早期门诊筛查。

我国几种日本血吸虫病免疫诊断试剂的综合测评

目的 了解我国血吸虫病防治工作中使用的免疫诊断试剂的综合质量,为第三次全国血吸虫病流行病学抽样调查查病工具的筛选提供参考。方法 聘请血吸虫病免疫诊断及相关专家组成专家评估组,确定综合测评指标。采用单盲法,由各日本血吸虫病免疫诊断试剂的生产单位对2 5 4份血清统一进行测试。计算各试剂盒的敏感性、特异性、Youden指数,收集其他测评指标的相关信息并进行分析。结果 参评的8个研究所(公司)的9种试剂盒总体上质量较好,大部分试剂盒的敏感性或特异性在90 %以上,其中Youden指数在0 .9以上的试剂盒有3个,但并殖吸虫病的交叉反应普遍存在,乙型肝炎的交叉反应在0～30 %之间。由于操作以及结果判断失误等其他原因,个别试剂盒的测试结果不够理想。结论 参加测评的绝大部分试剂盒的敏感性和特异性都达到了现场应用标准。