[Objective] The aim was to provide molecular biological basis for the researches on the genetic resources,genetic relationship among species and phyletic evolution of S.barcoo.[Method] PCR amplification and sequencing...[Objective] The aim was to provide molecular biological basis for the researches on the genetic resources,genetic relationship among species and phyletic evolution of S.barcoo.[Method] PCR amplification and sequencing were used to study the 16S rRNA and COI gene fragments.[Result] As for 16S rRNA gene fragments,nucleotide sequences of 791 bp were obtained,and the A,T,G and C contents in this fragment were 31.6%,21.4%,20.4% and 26.7%respectively.As for the COI gene fragments,the size was 631 bp and the A,T,G And C contents were 27.7%,23.6%,29.8% and 18.9% respectively.Among these two gene fragments,the content of GC was lower than AT,and AT/GC of these two fragments was 1.13 and 1.05 respectively.[Conclusion] The genetic characteristics of gene fragments of 16S rRNA and COI of S.barcoo suggested that the variation in the same species was relatively low.The sequences of 16S rRNA gene in three samples the same,while the sequences of COI gene was also the same,indicating that these two gene of S.barcoo were conservative.展开更多
Biofilter technology plays a key role in the production process of recirculating system. The establishment of biofilter′s full nitrifying function is very important to avoid the aquacultural hazards for producers. Th...Biofilter technology plays a key role in the production process of recirculating system. The establishment of biofilter′s full nitrifying function is very important to avoid the aquacultural hazards for producers. There were many reports about the cultivation of the biofilter in the recirculating system. However, almost all of them were carried out in the lab scale and had little meaning to the production scale. This paper compared three methods of cultivating biofilter in the recirculating aquacultural system. The results showed that putting appropriate load fish directly and feed lowly was the best method to establish the nitrification function of the biofilter in the production of the recirculating aquaculture system.展开更多
基金Supported by Agricultural Seed Project of Shandong Province~~
文摘[Objective] The aim was to provide molecular biological basis for the researches on the genetic resources,genetic relationship among species and phyletic evolution of S.barcoo.[Method] PCR amplification and sequencing were used to study the 16S rRNA and COI gene fragments.[Result] As for 16S rRNA gene fragments,nucleotide sequences of 791 bp were obtained,and the A,T,G and C contents in this fragment were 31.6%,21.4%,20.4% and 26.7%respectively.As for the COI gene fragments,the size was 631 bp and the A,T,G And C contents were 27.7%,23.6%,29.8% and 18.9% respectively.Among these two gene fragments,the content of GC was lower than AT,and AT/GC of these two fragments was 1.13 and 1.05 respectively.[Conclusion] The genetic characteristics of gene fragments of 16S rRNA and COI of S.barcoo suggested that the variation in the same species was relatively low.The sequences of 16S rRNA gene in three samples the same,while the sequences of COI gene was also the same,indicating that these two gene of S.barcoo were conservative.
文摘Biofilter technology plays a key role in the production process of recirculating system. The establishment of biofilter′s full nitrifying function is very important to avoid the aquacultural hazards for producers. There were many reports about the cultivation of the biofilter in the recirculating system. However, almost all of them were carried out in the lab scale and had little meaning to the production scale. This paper compared three methods of cultivating biofilter in the recirculating aquacultural system. The results showed that putting appropriate load fish directly and feed lowly was the best method to establish the nitrification function of the biofilter in the production of the recirculating aquaculture system.
