Previous experimental studies have shown that cerebral infarction can be effectively reduced following treatment with scutellaria baicalensis stem-leaf total flavonoid （SSTF）. However, the mechanism of action of SST...Previous experimental studies have shown that cerebral infarction can be effectively reduced following treatment with scutellaria baicalensis stem-leaf total flavonoid （SSTF）. However, the mechanism of action of SSTF as a preventive drug to treat cerebral infarction remains unclear. In this study, Sprague-Dawley rats were pretreated with 50, 100, 200 mg/kg SSTF via intragastric ad- ministration for 1 week prior to the establishment of focal cerebral ischemia/reperfusion injury. The results showed that pretreatment with SSTF effectively improved neurological function, reduced brain water content and the permeability of blood vessels, ameliorated ischemia-induced morphology changes in hippocampal microvessels, down-regulated Fas and FasL protein expression, elevated the activity of superoxide dismutase and glutathione peroxidase, and decreased malondialdehyde content. In contrast to low-dose SSTF pretreatment, the above changes were most obvious after pretreatment with moderateand high-doses of SSTF. Experimental findings indicate that SSTF pretreatment can exert protective effects on the brain against cerebral ischemia/reperfusion injury. The underlying mechanisms may involve reducing brain water content, increasing microvascular recanalization, inhibiting the apoptosis of hippocampal neurons, and attenuating free radical damage.展开更多
目的:探讨黄芩茎叶总黄酮(scutellaria baicalensis stem-leaf total flavonoid,SSTF)预处理对大鼠缺血再灌注损伤神经元的保护作用及其机制。方法:采用不同剂量SSTF预处理实验大鼠,制备局灶性脑缺血再灌注模型,参照Zea Longa 5分制评...目的:探讨黄芩茎叶总黄酮(scutellaria baicalensis stem-leaf total flavonoid,SSTF)预处理对大鼠缺血再灌注损伤神经元的保护作用及其机制。方法:采用不同剂量SSTF预处理实验大鼠,制备局灶性脑缺血再灌注模型,参照Zea Longa 5分制评分标准进行神经功能缺损评分,氯化三苯四氮唑(Triphenytetrazoliumchloride,TTC)染色法观测大鼠脑组织梗塞体积,免疫组化法检测大脑神经元凋亡基因蛋白的表达,电镜下观察大脑神经元超微结构损伤变化。结果:不同剂量的SSTF可不同程度减轻神经功能缺损;明显上调Bcl-2基因蛋白表达,下调Bax蛋白表达;减轻神经元各种细胞器特别是线粒体损伤,且随剂量加大改善越明显。结论:SSTF预处理可减轻缺血再灌注所致的神经元损伤,具有预防性保护作用,其机制可能与抑制细胞凋亡有关。展开更多
基金supported by the grants from Hebei Provincial Science and Technology Department,No.07276101D-46
文摘Previous experimental studies have shown that cerebral infarction can be effectively reduced following treatment with scutellaria baicalensis stem-leaf total flavonoid （SSTF）. However, the mechanism of action of SSTF as a preventive drug to treat cerebral infarction remains unclear. In this study, Sprague-Dawley rats were pretreated with 50, 100, 200 mg/kg SSTF via intragastric ad- ministration for 1 week prior to the establishment of focal cerebral ischemia/reperfusion injury. The results showed that pretreatment with SSTF effectively improved neurological function, reduced brain water content and the permeability of blood vessels, ameliorated ischemia-induced morphology changes in hippocampal microvessels, down-regulated Fas and FasL protein expression, elevated the activity of superoxide dismutase and glutathione peroxidase, and decreased malondialdehyde content. In contrast to low-dose SSTF pretreatment, the above changes were most obvious after pretreatment with moderateand high-doses of SSTF. Experimental findings indicate that SSTF pretreatment can exert protective effects on the brain against cerebral ischemia/reperfusion injury. The underlying mechanisms may involve reducing brain water content, increasing microvascular recanalization, inhibiting the apoptosis of hippocampal neurons, and attenuating free radical damage.
文摘目的:探讨黄芩茎叶总黄酮(scutellaria baicalensis stem-leaf total flavonoid,SSTF)预处理对大鼠缺血再灌注损伤神经元的保护作用及其机制。方法:采用不同剂量SSTF预处理实验大鼠,制备局灶性脑缺血再灌注模型,参照Zea Longa 5分制评分标准进行神经功能缺损评分,氯化三苯四氮唑(Triphenytetrazoliumchloride,TTC)染色法观测大鼠脑组织梗塞体积,免疫组化法检测大脑神经元凋亡基因蛋白的表达,电镜下观察大脑神经元超微结构损伤变化。结果:不同剂量的SSTF可不同程度减轻神经功能缺损;明显上调Bcl-2基因蛋白表达,下调Bax蛋白表达;减轻神经元各种细胞器特别是线粒体损伤,且随剂量加大改善越明显。结论:SSTF预处理可减轻缺血再灌注所致的神经元损伤,具有预防性保护作用,其机制可能与抑制细胞凋亡有关。
