Brine shrimp cytotoxic activities of Hippophae rhamnoides Linn leaves extracts

Objective:To evaluate brine shrimp lethality assay of solvent extracts(aqueous,methanol,ethanol,acetone,ethyl acetate,chloroform and n-hexane)of Hippophae rhamnoides(H.rhamnoides)leaves.Methods:Brine shrimp cytotoxicity assay was used to assess the cytotoxic potential of H.rhamnoides leaves extracts.Three vials for concentration of each extract were made and 10 shrimps per vial(30 shrimps per dilution)were transferred to specific concentration of each extract.Results:The mortality of aqueous extract was 46.7%,methanol extract was 46.7%,ethanolic extract was 50.0%,ethyl acetate was 26.7%,acetone extract was 33.3%,chloroform extract was 40.0%and n-hexane extract was 33.3%.The lowest LD50 was found in methanol extracts(1199.97μg/mL).Brine shrimp cytotoxicity of tested extracts of H.rhamnoides showed that mortality rate was concentration dependent.Conclusions:It is concluded that bioactive components are present in all leaves extracts of H.rhamnoides,which could be accounted for its pharmacological effects.Thus,the results support the uses of this plant species in traditional medicine.