Extraction and purification of TGFβ and its effect on the induction of apoptosis of hepatocytes

AIM To extract and purify the transforming growth factor β (TGF β), and to demonstrate its biological activity in vivo and induction of apoptosis of hepatocytes in vitro.METHODS TGF β was isolated from fresh bovine platelets by acid/ethanol extraction method and purified with ion exchange and gel chromatography. The extracted TGF β was injected subcutaneously to mice, and its biological activity in vivo was observed 72 hfs post-injection by HE staining. The morphological changes were observed by HE staining and the occurrence of apoptosis was detected by TUNEL method after the human normal hepatic cell line QZG was treated with 8 μg@L 1 TGFβ for 12 hrs in vitro.RESULTS The molecular mass 25 ku TGF β protein was successfully extracted. It was able to induce localized granulation tissue formation in vivo. TGF β-treated hepatocytes showed obvious apoptotic morphological changes, including the pyknosis and dense-stained nuclei and cytoplasm, the fragmentary, annular or crescent nuclei, and the "bubbling" cytoplasm. Moreover, its apoptotic rate was significantly higher than that of the control group (P＜0.05).CONCLUSION Biological active TGF β protein is extracted and purified successfully from bovine platelets, and it is able to induce the apoptosis of hepatocytes.

海藻硫酸多糖的分离纯化及其抗肿瘤作用

目的:探讨海藻硫酸多糖的分离纯化及其抗肿瘤作用。方法:选取鼠龄、质量大致相同的昆明种雄性小鼠60只,随机分为5组,正常对照组、阳性对照组、低剂量组、中剂量组和高剂量组,每组12只。低剂量组、中剂量组和高剂量组分别注射浓度为25 mg/kg、50 mg/kg、100 mg/kg的海藻硫酸多糖,氟脲嘧啶20 mg/kg注射阳性对照组,正常对照组采用生理盐水溶液注射,比较各组小鼠的抑瘤率。结果:低剂量组、中剂量组、高剂量组和阳性对照组小鼠的抑瘤率分别为5.0%、22.3%、27.9%和50.3%,阳性对照组的抑瘤率明显高于其他三组,高剂量组的抑瘤率明显高于低剂量组和中剂量组,中剂量组的抑瘤率明显高于低剂量组,组间比较差异具有统计学意义(P<0.05)。结论:海藻硫酸多糖具有显著的抗肿瘤作用。

海藻酸钠纯度对微囊纤维化及囊内甲状旁腺组织功能的影响

目的研究海藻酸纯度对移植微囊及囊内人胚胎甲状旁腺组织功能的影响。方法将纯化及未纯化海藻酸微囊及微囊化人胚胎甲状旁腺组织分 4组异种移植于小鼠腹腔内 ,设立空囊对照组。测定人甲状旁腺素数值 ,通过测定具有特异性的人甲状旁腺素 ,以明确微囊内甲状旁腺组织的分泌功能 ,组织学检测微囊形态。结果移植后 2个月纯化组及未纯化组人甲状旁腺组织分泌功能有显著差异 (P <0 .0 5 ) ,分别为 (38.8± 13.7) pg/ml和(1.2± 0 .7) pg/ml,纯化及未纯化海藻酸钠制备空囊移植组 ,植入 1个月时微囊冲洗回收率分别为 (72± 18) %和(2 4± 13) % ,植入 2个月时为 (6 5± 2 1) %和 0。结论纯化的海藻酸制备微囊具有良好的生物相容性 。

Studies of Coltivirus in China

Objective The purpose of this article is to review the developments of studies of Coltivirus in ChinaData sources The data used in this review was obtained mainly from the studies of Coltivirus reported from 1990 to 2003 in China.Study selection Relevant articles on studies of Coltivirus in domestic and foreign literature were selected.Data extraction Data were maily extracted from the articles which are listed in the reference section of this review.Results Many Coltiviruses have been isolated not only from blood samples of patients with unknown fever or from cerebrospinal fluid of patients with encephalitis in Xishuangbanna area in Yunnan province, but also from mosquitoes collected in many areas in China. In some patients diagnosed as Japanese encephalitis or unknown fever, an increase of Coltivirus IgG antibody of fourfold, or more, has been detected using ELISA. Similarly, Coltivirus IgM antibody was positive in some patients with Japanese encephalitis or viral encephalitis. From most Chinese patients, except the northeastern, the isolates of Coltiviruses belong to subgroup B2, according to RT-PCR amplification of the ninth and twelfth segments of the isolates and sequence analysis of their amplicons. Some biological properties of Chinese Coltiviruses isolates are different from that of North American Coltiviruses.Conclusions The isolates of Coltiviruses from Chinese patients are one of the common agents causing viral encephalitis and unknown fever in summer-autumn season. It might be an important public health problem due to its high isolation rate and wide distribution in China. Mosquito is the main transmission vector of the virus.