A new germacrane sesquiterpene was obtained from the roots of L. Dentata which was a folk herb medicine in China- Its structure was elucidated to be 1α, 4β, 6α, 10a-tetrahy-droxy-3β, 5a, 8a-triangeloyloxygermacr-1...A new germacrane sesquiterpene was obtained from the roots of L. Dentata which was a folk herb medicine in China- Its structure was elucidated to be 1α, 4β, 6α, 10a-tetrahy-droxy-3β, 5a, 8a-triangeloyloxygermacr-11-en-9-one with the spectroscopic techniques (such as IR, MS, 1H NMR, 13C NMR and 2D-NMR) and chemical transformation.展开更多
AIM To study the anticancer effects of two novel highly oxygenated bisabolance sesquiterpene compounds from Cremanthodium discoideum Maxim on the human hepatoma cell line SMMC-7721. METHODS In vitro, the cytotoxity of...AIM To study the anticancer effects of two novel highly oxygenated bisabolance sesquiterpene compounds from Cremanthodium discoideum Maxim on the human hepatoma cell line SMMC-7721. METHODS In vitro, the cytotoxity of compound 1, 2 was tested by MTT colorimetric assay, and the single-cell gel electrophoresis (SCGE) assay was used for assessing the DNA damage of SMMC-7721 cells induced by compound 1, 2. The apoptotic rate of SMMC-7721 cells was assayed by flow cytometry. RESULTS The IC 50 of SMMC-7721 cells exposed to the two compounds for 72 h were (37±7) μg·mL -1 and (22±9) μg·mL -1, respectively. The result of SCGE showed that the two compounds (2, 4 and 8 μg·mL -1, 48 h) were able to induce DNA damage in SMMC-7721 cells and showed dose-dependent effect. Flow cytometric analyses showed that after exposure to the two compounds for 48 h induced the apoptosis in SMMC-7721 cells. CONCLUSION The two novel highly oxygenated bisabolance sesquiterpene compounds showed significant inhibition effect on the growth of SMMC-7721 cells and its mechanism was probably related with the DNA damage and apoptosis induced by them.展开更多
Two furoeremophilanes (1) and (2) were isolated from Senecio kaschkarovii C.Winkl.growing in Zhang County of Gansu Province.Their structures were elucidated by UV, IR, MS, 1 H NMR and 13 C NMR spectral data as 1α ace...Two furoeremophilanes (1) and (2) were isolated from Senecio kaschkarovii C.Winkl.growing in Zhang County of Gansu Province.Their structures were elucidated by UV, IR, MS, 1 H NMR and 13 C NMR spectral data as 1α acetoxy 6β angeloyloxy 10β hydroxy 9 oxofuroeremophilane (1) and 1α methacryloyloxy 6β acetoxy 10β hydroxy 9 oxofuroeremophilane (2) respectively and were further established by 2D 1 H, 1 H NMR correlated spectrum, 1 H, 13 C NMR correlated spectrum and 1 H NOE difference spectrum.Compound 2 is a new furoeremophilane.展开更多
Plants of the Senecio Kaschkarovii are widely distributed in the southeast districts of Gansu province of China.In the past few years several sesquiterpenes had been isolated from this family compositae.On the basic o...Plants of the Senecio Kaschkarovii are widely distributed in the southeast districts of Gansu province of China.In the past few years several sesquiterpenes had been isolated from this family compositae.On the basic of the previous work,four furoeremophilanes were also isolated from Senecio kaschkarovii.Their structures, were identified as 1α-acetoxy-6β-angeloyloxy-10β-hydroxy-9-oxofuroeremophilane(1);1α-methacryloyloxy-6β-Acetoxy-10β-hydroxy-9-oxofuroeremophilane(2);6β-isobutyloxy-10(1)-en-9-oxofuroeremophilane(3);1β-acetoxy-6β-acetoxy-10β-H-9-oxofuroeremophilane(4) by means of spectroscopic techniques(UV,IR,NMR,MS,CD) and chemical properties,.Compound 3 was obtained from Senecio kaschkarovii for the first time.Compound 4 was a new compound,and its absolute configuration was determined.展开更多
文摘A new germacrane sesquiterpene was obtained from the roots of L. Dentata which was a folk herb medicine in China- Its structure was elucidated to be 1α, 4β, 6α, 10a-tetrahy-droxy-3β, 5a, 8a-triangeloyloxygermacr-11-en-9-one with the spectroscopic techniques (such as IR, MS, 1H NMR, 13C NMR and 2D-NMR) and chemical transformation.
文摘AIM To study the anticancer effects of two novel highly oxygenated bisabolance sesquiterpene compounds from Cremanthodium discoideum Maxim on the human hepatoma cell line SMMC-7721. METHODS In vitro, the cytotoxity of compound 1, 2 was tested by MTT colorimetric assay, and the single-cell gel electrophoresis (SCGE) assay was used for assessing the DNA damage of SMMC-7721 cells induced by compound 1, 2. The apoptotic rate of SMMC-7721 cells was assayed by flow cytometry. RESULTS The IC 50 of SMMC-7721 cells exposed to the two compounds for 72 h were (37±7) μg·mL -1 and (22±9) μg·mL -1, respectively. The result of SCGE showed that the two compounds (2, 4 and 8 μg·mL -1, 48 h) were able to induce DNA damage in SMMC-7721 cells and showed dose-dependent effect. Flow cytometric analyses showed that after exposure to the two compounds for 48 h induced the apoptosis in SMMC-7721 cells. CONCLUSION The two novel highly oxygenated bisabolance sesquiterpene compounds showed significant inhibition effect on the growth of SMMC-7721 cells and its mechanism was probably related with the DNA damage and apoptosis induced by them.
文摘Two furoeremophilanes (1) and (2) were isolated from Senecio kaschkarovii C.Winkl.growing in Zhang County of Gansu Province.Their structures were elucidated by UV, IR, MS, 1 H NMR and 13 C NMR spectral data as 1α acetoxy 6β angeloyloxy 10β hydroxy 9 oxofuroeremophilane (1) and 1α methacryloyloxy 6β acetoxy 10β hydroxy 9 oxofuroeremophilane (2) respectively and were further established by 2D 1 H, 1 H NMR correlated spectrum, 1 H, 13 C NMR correlated spectrum and 1 H NOE difference spectrum.Compound 2 is a new furoeremophilane.
文摘Plants of the Senecio Kaschkarovii are widely distributed in the southeast districts of Gansu province of China.In the past few years several sesquiterpenes had been isolated from this family compositae.On the basic of the previous work,four furoeremophilanes were also isolated from Senecio kaschkarovii.Their structures, were identified as 1α-acetoxy-6β-angeloyloxy-10β-hydroxy-9-oxofuroeremophilane(1);1α-methacryloyloxy-6β-Acetoxy-10β-hydroxy-9-oxofuroeremophilane(2);6β-isobutyloxy-10(1)-en-9-oxofuroeremophilane(3);1β-acetoxy-6β-acetoxy-10β-H-9-oxofuroeremophilane(4) by means of spectroscopic techniques(UV,IR,NMR,MS,CD) and chemical properties,.Compound 3 was obtained from Senecio kaschkarovii for the first time.Compound 4 was a new compound,and its absolute configuration was determined.
