MULTIPHOTON MICROSCOPIC IMAGING OF MOUSE INTESTINAL MUCOSA BASED ON TWO-PHOTON EXCITED FLUORESCENCE AND SECOND HARMONIC GENERATION

Multiphoton microscopy(MPM),based on two-photon excited fuorescence and second harmonic generation,enables direct noninvasive visualization of tissue architecture and cell morphology in live tissues without the administration of exogenous contrast agents.In this paper,we used MPM to image the microstructures of the mucosa in fresh,unfixed,and unstained intestinal tissue of mouse.The morphology and distribution of the main components in mucosa layer such as columnar cells,goblet cells,intestinal glands,and a little collagen fibers were clearly observed in MPM images,and then compared with standard H&:E images from paired specimens.Our results indicate that MPM combined with endoscopy and miniaturization probes has the potential application in the clinical diagnosis and in vivo monitoring of early intestinal cancer.

基于非线性光学显微镜无标记探测胃粘膜肠上皮化生

胃粘膜肠上皮化生被广泛认为是胃癌的前兆。早期发现和准确诊断将具有重要的临床意义。在本工作中,我们提出采用非线性光学显微镜对胃粘膜肠上皮化生进行无标记探测。成像结果表明,该技术有能力区分正常和病变组织,并能进一步区分肠上皮化生的亚型。此外,我们分别测量正常和异常组织上皮细胞的核面积,以说明胃黏膜从正常到肠上皮化生发展过程中的细胞形态变化。本研究说明非线性光学显微镜具备诊断胃粘膜肠上皮化生的潜力,将为进一步研究这种病变提供一种新方法。