The increasing importance of endothelium-derived relaxing factor(EDRF),which has now been identified as nitric oxide (NO),has been underscored by the eltlcidation of its role'in a growing number of normal and path...The increasing importance of endothelium-derived relaxing factor(EDRF),which has now been identified as nitric oxide (NO),has been underscored by the eltlcidation of its role'in a growing number of normal and pathophysiological processes. Therefore techniques for detection of nitric oxide should serve as useful tools in defining the role of nitric oxide to these processes.We have improved a simple, sensitive assay methods for determination of nitric oxide in blood, tissue, and other body fluids both by fluorometric and by ultraviolet-visible spectrophotometric measurements. Data obtained by floores cence and by UV-visible assay were correlated well (r=0. 9938, P<0. 0001 ).Linearity:0.1￣ 100μmol/L,r =0.9996,P<0.0001. The minimum detection limit were < 10pmol/L. Within-and between-run CVs were 2. 48%and 4. 62% (n = 10),respectively.Reference values for healthy adults(n=40) were(9.82 ± 1. 57) pmol/L. In conclusion:the methods is sensitive, specific,and precise. It is fairly rapid and simple to perform andrequires no pretreatment of sample, i. e., plasma and urine.The value can be obtained by fluorimeter and/or UV-visible spectrophotometer.The present method is sufficiently rapid and simple to make this a practical choice for many laboratories.展开更多
文摘The increasing importance of endothelium-derived relaxing factor(EDRF),which has now been identified as nitric oxide (NO),has been underscored by the eltlcidation of its role'in a growing number of normal and pathophysiological processes. Therefore techniques for detection of nitric oxide should serve as useful tools in defining the role of nitric oxide to these processes.We have improved a simple, sensitive assay methods for determination of nitric oxide in blood, tissue, and other body fluids both by fluorometric and by ultraviolet-visible spectrophotometric measurements. Data obtained by floores cence and by UV-visible assay were correlated well (r=0. 9938, P<0. 0001 ).Linearity:0.1￣ 100μmol/L,r =0.9996,P<0.0001. The minimum detection limit were < 10pmol/L. Within-and between-run CVs were 2. 48%and 4. 62% (n = 10),respectively.Reference values for healthy adults(n=40) were(9.82 ± 1. 57) pmol/L. In conclusion:the methods is sensitive, specific,and precise. It is fairly rapid and simple to perform andrequires no pretreatment of sample, i. e., plasma and urine.The value can be obtained by fluorimeter and/or UV-visible spectrophotometer.The present method is sufficiently rapid and simple to make this a practical choice for many laboratories.
