As an important carrier of red culture,red music culture carries the glorious history of revolution,construction,and reform led by the Communist Party of China(CPC),and has significant functional value in cultivating ...As an important carrier of red culture,red music culture carries the glorious history of revolution,construction,and reform led by the Communist Party of China(CPC),and has significant functional value in cultivating students’ideals,values,and cultural identity,as well as patriotic consciousness and national pride.However,the integration of red music culture into the ideological and political education of primary and secondary schools faces the dilemmas of low cultivation awareness,insufficient excavation of resources,and lack of optimization of atmosphere and field.In view of these problems,the strategies of enhancing cultivation awareness,excavating the resources in-depth,and creating a positive atmosphere and field provide new ideas and methods for ideological and political education in primary and secondary schools.展开更多
Tissue culture techniques were used to produce large amounts of bioactive compounds with medicinal potential, overcoming space and time constraints for cancer prevention. Rice callus suspension cultures(RCSC) and seed...Tissue culture techniques were used to produce large amounts of bioactive compounds with medicinal potential, overcoming space and time constraints for cancer prevention. Rice callus suspension cultures(RCSC) and seed extracts prepared from aromatic rice varieties were used to evaluate the cytotoxic impact on human colon and lung cancer cell lines, as well as a normal control cell line, using Taxol as a positive control. RCSC and seed extracts from two Indian aromatic rice varieties were applied at different concentrations to treat the cancer cell lines and normal lung fibroblasts over varying time intervals. Apoptosis was assessed in 1:5 dilutions of the A549 and HT-29 cell lines treated with RCSC for 72 h, using propidium iodide staining and flow cytometry. RCSC showed a more potent cytotoxic effect than seed extracts with minimal effect on the normal cell line, in contrast to Taxol. Confocal microscopy and flow cytometry further confirmed the apoptotic effect of RCSC. Gas chromatography-mass spectrometry-based metabolic profiling identified metabolites involved in cytotoxicity and highlighted altered pathways. RCSC is proposed as an alternative source for the development of novel anticancer drugs with reduced side effects.展开更多
Plant cell culture technology is a technology that applies the research results of cell engineering to produce plant biological products at the cellular level.In recent years,the secondary metabolites of plants have a...Plant cell culture technology is a technology that applies the research results of cell engineering to produce plant biological products at the cellular level.In recent years,the secondary metabolites of plants have attracted more and more attention.The use of plant cell culture technology is a fast and efficient method of producing secondary metabolites.展开更多
Diabetes is chronic metabolic disorder characterized by states of hyperglycemia with disturbances of carbohydrates, fat and protein metabolism. Diabetes affects millions of people globally every day and the prevalence...Diabetes is chronic metabolic disorder characterized by states of hyperglycemia with disturbances of carbohydrates, fat and protein metabolism. Diabetes affects millions of people globally every day and the prevalence of the disease is on the rise due to unhealthy diet and lifestyle. The disorder usually results to chronic complications including cardiovascular diseases, diabetic nephropathy, diabetic neuropathy, foot ulcers and diabetic eye diseases that are all preventable through secondary preventive measures. Once an individual has been diagnosed with T2DM, secondary preventive approaches are essential in preventing the occurrence of chronic complications. However, lack of awareness of these measures has been cited as the common reasons for the development of complications. The study aimed to assess the effect of social cultural and economic factors on the practice of secondary diabetes prevention among patients with Type 2 Diabetes Mellitus (T2DM) at Consolata Hospital Nkubu and Meru Level Five Hospital between March and April 2019. A descriptive correlational study design was adopted to collect data from 357 purposively sampled participants with T2DM using questionnaires and Focus Group Discussion Guide. Quantitative data were analyzed using SPSS version 25 at 95% confidence interval and a significance level p ≤ 0.05. Most respondents attended Meru Teaching and Referral Hospital. Majority of the respondents were aged between 40 - 60 years. Most respondents 31.6% had secondary level of education and majority 67% was employed. Concerning secondary prevention, majority did foot examination on every visit 70.6% and BP monitoring 69.5% while 56.5% did annual eye screening. Level of income, affordability of services, health insurance cover of the patients, monthly cost of DM management and traditional beliefs in managing DM all significantly influenced DM secondary prevention at a p value ≤ 0.05. The factors need to be addressed to reduce the global burden posed by the disease.展开更多
Callus cultures of Annona muricata and Annona purpurea were induced in Murashige and Skoog(MS)medium supplemented with different concentrations of 1-naphthylacetic acid(NAA),6-benzyladenine(BA)and 2,4-dichlorophenoxya...Callus cultures of Annona muricata and Annona purpurea were induced in Murashige and Skoog(MS)medium supplemented with different concentrations of 1-naphthylacetic acid(NAA),6-benzyladenine(BA)and 2,4-dichlorophenoxyacetic acid(2,4-D)utilized hypocotyls with explant.The highest percentage of callus formation was the treatment supplemented with 3 mg L-1 NAA for A.muricata(100%)while for A.purpurea in lower percentage(75%).BA stimulated the formation of shoots in all the evaluated concentrations,being the concentration of 2 mg L-1 the one that induced the greater formation of shoots for A.muricata(23 shoots/explant)and A.purpurea(28 shoots/explant).The content of total phenols,flavonoids and antioxidant activity was measured in the callus obtained from both species.The results showed that a higher content of total phenols was quantified in callus of A.purpurea(27.8 mg g-1 dw)compared to A.muricata(23.2 mg g-1 dw).The highest content of total flavonoids was observed in the callus of A.purpurea(8.0μg g-1 dw).Antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydracil radical assay.The concentration required for 50%inhibition(IC50)of the 2,2-diphenyl-1-picrylhydracil radicals were 4.22μg mL-1 in methanolic extracts of callus of A.muricata,while in extracts of callus of A.purpurea was 2.86μg mL-1,in both cases was greater than that found for leaves.Callus culture of the species studied in this work represents an alternative for the production of natural antioxidants.展开更多
Chinese excellent traditional culture contains the value pursuit of the Chinese nation,condenses the cultural consensus of the Chinese nation,and has rich connotation and connotation.At the moment of advocating the in...Chinese excellent traditional culture contains the value pursuit of the Chinese nation,condenses the cultural consensus of the Chinese nation,and has rich connotation and connotation.At the moment of advocating the integration of ideological and political courses in primary and secondary schools,the integration of Chinese traditional culture into the curriculum content of ideological and political courses can have a subtle cultural impact on the students of different students,and help students to grow up physically and mentally and cultivate their ideology and morality.In order to achieve ideological and political curriculum implicit development.展开更多
文摘As an important carrier of red culture,red music culture carries the glorious history of revolution,construction,and reform led by the Communist Party of China(CPC),and has significant functional value in cultivating students’ideals,values,and cultural identity,as well as patriotic consciousness and national pride.However,the integration of red music culture into the ideological and political education of primary and secondary schools faces the dilemmas of low cultivation awareness,insufficient excavation of resources,and lack of optimization of atmosphere and field.In view of these problems,the strategies of enhancing cultivation awareness,excavating the resources in-depth,and creating a positive atmosphere and field provide new ideas and methods for ideological and political education in primary and secondary schools.
基金partly funded by the Department of Science and Technology Fund for Improvement of S&T Infrastructure (Grant No. SR/FST/LS-I/2018/125)。
文摘Tissue culture techniques were used to produce large amounts of bioactive compounds with medicinal potential, overcoming space and time constraints for cancer prevention. Rice callus suspension cultures(RCSC) and seed extracts prepared from aromatic rice varieties were used to evaluate the cytotoxic impact on human colon and lung cancer cell lines, as well as a normal control cell line, using Taxol as a positive control. RCSC and seed extracts from two Indian aromatic rice varieties were applied at different concentrations to treat the cancer cell lines and normal lung fibroblasts over varying time intervals. Apoptosis was assessed in 1:5 dilutions of the A549 and HT-29 cell lines treated with RCSC for 72 h, using propidium iodide staining and flow cytometry. RCSC showed a more potent cytotoxic effect than seed extracts with minimal effect on the normal cell line, in contrast to Taxol. Confocal microscopy and flow cytometry further confirmed the apoptotic effect of RCSC. Gas chromatography-mass spectrometry-based metabolic profiling identified metabolites involved in cytotoxicity and highlighted altered pathways. RCSC is proposed as an alternative source for the development of novel anticancer drugs with reduced side effects.
文摘Plant cell culture technology is a technology that applies the research results of cell engineering to produce plant biological products at the cellular level.In recent years,the secondary metabolites of plants have attracted more and more attention.The use of plant cell culture technology is a fast and efficient method of producing secondary metabolites.
文摘Diabetes is chronic metabolic disorder characterized by states of hyperglycemia with disturbances of carbohydrates, fat and protein metabolism. Diabetes affects millions of people globally every day and the prevalence of the disease is on the rise due to unhealthy diet and lifestyle. The disorder usually results to chronic complications including cardiovascular diseases, diabetic nephropathy, diabetic neuropathy, foot ulcers and diabetic eye diseases that are all preventable through secondary preventive measures. Once an individual has been diagnosed with T2DM, secondary preventive approaches are essential in preventing the occurrence of chronic complications. However, lack of awareness of these measures has been cited as the common reasons for the development of complications. The study aimed to assess the effect of social cultural and economic factors on the practice of secondary diabetes prevention among patients with Type 2 Diabetes Mellitus (T2DM) at Consolata Hospital Nkubu and Meru Level Five Hospital between March and April 2019. A descriptive correlational study design was adopted to collect data from 357 purposively sampled participants with T2DM using questionnaires and Focus Group Discussion Guide. Quantitative data were analyzed using SPSS version 25 at 95% confidence interval and a significance level p ≤ 0.05. Most respondents attended Meru Teaching and Referral Hospital. Majority of the respondents were aged between 40 - 60 years. Most respondents 31.6% had secondary level of education and majority 67% was employed. Concerning secondary prevention, majority did foot examination on every visit 70.6% and BP monitoring 69.5% while 56.5% did annual eye screening. Level of income, affordability of services, health insurance cover of the patients, monthly cost of DM management and traditional beliefs in managing DM all significantly influenced DM secondary prevention at a p value ≤ 0.05. The factors need to be addressed to reduce the global burden posed by the disease.
文摘Callus cultures of Annona muricata and Annona purpurea were induced in Murashige and Skoog(MS)medium supplemented with different concentrations of 1-naphthylacetic acid(NAA),6-benzyladenine(BA)and 2,4-dichlorophenoxyacetic acid(2,4-D)utilized hypocotyls with explant.The highest percentage of callus formation was the treatment supplemented with 3 mg L-1 NAA for A.muricata(100%)while for A.purpurea in lower percentage(75%).BA stimulated the formation of shoots in all the evaluated concentrations,being the concentration of 2 mg L-1 the one that induced the greater formation of shoots for A.muricata(23 shoots/explant)and A.purpurea(28 shoots/explant).The content of total phenols,flavonoids and antioxidant activity was measured in the callus obtained from both species.The results showed that a higher content of total phenols was quantified in callus of A.purpurea(27.8 mg g-1 dw)compared to A.muricata(23.2 mg g-1 dw).The highest content of total flavonoids was observed in the callus of A.purpurea(8.0μg g-1 dw).Antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydracil radical assay.The concentration required for 50%inhibition(IC50)of the 2,2-diphenyl-1-picrylhydracil radicals were 4.22μg mL-1 in methanolic extracts of callus of A.muricata,while in extracts of callus of A.purpurea was 2.86μg mL-1,in both cases was greater than that found for leaves.Callus culture of the species studied in this work represents an alternative for the production of natural antioxidants.
文摘Chinese excellent traditional culture contains the value pursuit of the Chinese nation,condenses the cultural consensus of the Chinese nation,and has rich connotation and connotation.At the moment of advocating the integration of ideological and political courses in primary and secondary schools,the integration of Chinese traditional culture into the curriculum content of ideological and political courses can have a subtle cultural impact on the students of different students,and help students to grow up physically and mentally and cultivate their ideology and morality.In order to achieve ideological and political curriculum implicit development.